Synthetic gene circuit allows precise dosing of gene expression
... with a promoter that launches the tetracycline repressor
gene, which then blocks
yEGFP via a promoter for ... in measured doses stifles the tetracycline repressor
and permits expression of yEGFP.
When ATc was ...
The team then made the promoter for the repressor
identical to that used for yEGFP. This, in ...
A new relationship between brain derived neurotrophic factor and inflammatory signaling
... 1 gene regulation is dependent upon the induction of a transcriptional repressor
called inducible cAMP early repressor
(ICER) that binds to the alpha 1 gene in coordination with the cAMP ...
NYU Langone Medical Center's tip sheet to the 44th annual meeting of ASCO
... # 5065
Androgen receptor (AR) overexpression and sensitivity to hormones reversed by epigenetic therapy that restores Pura to a transcriptional repressor
complex (RC) of AR deregulated in hormone refractory prostate cancer (HRPC).
NYU Cancer Institutes Anna Ferrari, M.D., associate professor of ...
Novel mechanism found that may boost impaired function of leukemia protein
... researching the AML1-ETO protein at MSKCC since 1993. He and his colleagues first demonstrated in 1995 that AML1-ETO functions as a transcriptional repressor
and dominantly inhibits AML1 function.
JCI online early table of contents: Jan. 2, 2008
... authors therefore concluded from these data that AHRR is a tumor suppressor gene present in various tissues.
TITLE: The aryl hydrocarbon receptor repressor
is a putative tumor suppressor gene in multiple human cancers
National Cancer Institute, Bethesda, Maryland, ...
JCI table of contents: Nov. 15, 2007
... these data led the authors to conclude that FOXP3 is a suppressor of tumor development induced by SKP2.
TITLE: FOXP3 is a novel transcriptional repressor
for the breast cancer oncogene SKP2
University of Michigan, Ann Arbor, Michigan, USA.
Phone: (734) 615-3158; Fax: ...
Insights into Acute Promyelocytic Leukemia
... protein called a fusion protein.
In APL, the fusion protein, always involving the transcription factor RARA, acts as a potent transcriptional repressor
that interferes with gene expression and prevents normal differentiation of white blood cells. Previous work of these two groups suggested that ...
New Target for Muscular Dystrophy Drug Therapy Discovered
... dystrophin as a possible therapy for DMD.
Experiments on mice have now shown that utrophin silencing is applied by a protein called Ets-2 repressor
factor (ERF) sitting on a small piece of the utrophin gene called the N-box.
"We demonstrated that ERF significantly reduces or represses the ...
Molecular ‘On/Off Switch’ Controls Immune Defenses Against Viruss
... is normally regulated, however, hadn’t been known.
In the current study, UT Southwestern researchers found that RIG-I and LGP2 each contain a repressor
domain, a sort of docking site that controls the actions of each protein. The domain is the key site that regulates the ability of RIG-I to bind to ...
Repressor in Biological News
The evolution of gene regulation
... manner, on the other hand, are kept inactive by a repressor
that is removed only when the gene is needed. ... key fits the lock to this protein, to detach the repressor
from the lactase gene: a lactose molecule, as a ... genetic codes that are only released from their repressor
when needed." The "use-it-or-lose-it" principle ...
Evolution of fruit size in tomato
... sequencing of this locus reveals that the wild type protein codes for a repressor
of cell division. When the control sequence is mutated, the repressor
protein is not expressed or only very little, leading to higher cell ...
Xie Lab uncovers molecular machinery related to stem cell fate
... participate in stem cell misregulation and the development of tumors."
"Our studies have established the role of BAM as a protein translational repressor
using biochemical and genetic tests," said Ting Xie, Ph.D., Investigator and senior author on the paper. "Translational control is very important in ...
6 young ASU faculty earn NSF Career awards
... is involved in embryonic development, angiogenesis and cancer), which partially unfolds upon binding and bends the DNA by 26 degrees; and the lac repressor
headpiece (part of a much larger bacterial protein), which folds upon binding and bends the DNA by 36 degrees.
Computer simulations will give ...
A study reveals how cells communicate to activate the cell division machinery
... elucidated the relationship between Notch and Wnt/Wingless in the control of proliferation and the development of the fly wing. In fact, Notch has a repressor
function, that is to say, when it is activated the cell division machinery is arrested. Only when Wnt/Wingless starts to work is Notch silenced, ...
New technique yields more detailed picture of chromatin structure
... the chromosomes. A bacterial protein, the Lac repressor, recognizes and binds to the Lac operator in living cells.
The researchers combined a Lac repressor
protein with another protein that fluoresces green under blue light. This engineered protein adhered to the chromosomes in regions containing the Lac ...
Root or shoot? EAR calls the shots
... plant to have its genome unlocked and is loved by plant biologists for its short generation time.
She discovered that BODENLOS, a transcriptional repressor
that silences auxin-responsive genes, relies on its EAR domain to recruit the co-repressor TOPLESS to help with the job. While auxins are found ...
3 Columbia University Medical Center faculty elected to Institute of Medicine
... work focuses primarily on transcriptional regulation of lymphocyte development. A major focus of her current work is an unusual transcriptional repressor
called Blimp-1 (B lymphocyte induced maturation protein). Her studies on Blimp-1 are revealing important aspects of regulation in both B and T cells. ...
Bioinformatics reveals new gene regulation system
... transcription factor that bound it, the repressor
nature of the signal, and other genes also ... sequence, which lies just outside of the gene. repressor
signals commonly work by allowing other proteins ... duo predicted that the NrdR-box functioned as a repressor
Next, the researchers identified the ...
A simple feedback resistor switch keeps latent HIV from awakening
Certain bacterial viruses manage this problem by encoding intricate repressor
circuits that efficiently block transcription. But animal viruses, specifically HIV, appear to lack similar repressor
circuits. In a new study, published online in the open access journal PLoS ...
Repressor in Biological Technology
JCI table of contents: April 22, 2008
... by a reliance on either cyclin D1 or cyclin D2, such as many individuals with multiple myeloma.
TITLE: Identification of kinetin riboside as a repressor
of CCND1 and CCND2 with preclinical antimyeloma activity
A. Keith Stewart
Mayo Clinic, Scottsdale, Arizona, USA.
Phone: (480) ...
Functional Cloning Using
ViraPort Retroviral cDNA Expression Libraries
of known cell-cycle regulatory protein
(to be discussed in a ... 13. Onco-1 contained a gene encoding a
of a known cell cycle regulatory protein. It
is not surprising, ...
Sequence-Validated and Expression-Tested Human cDNA in a Dual Expression
... features designed for
inducible high-level protein expression in bacterial cells. The vector contains
the hybrid T7/lac O promoter and lac repressor
gene (lac I)
to regulate protein expression. Therefore, expression is inducible using
isopropyl-1-thio-b-D-galatopyranoside (IPTG) in
Mammalian and Bacterial Expression in One Vector
... cells. Expression of heterologous genes in bacteria is
regulated by a hybrid T7/ lacO promoter. The pDual vector was engineered
to carry the lac repressor
gene (lacI). Expression is therefore inducible
using IPTG in bacteria that contain the T7 RNA polymerase. ##
In both bacterial and mammalian ...
pFB-ERV: Retroviral Delivery of the Ecdysone Receptor Proteins
... repressed until ligand binding, at which time
high-level transcriptional activation occurs (i.e., the heterodimer is converted
from a tight repressor
to a trans-activator). Using the Complete Control
plasmid-based system, induction ratios of greater than 1,000-fold have been
achieved in both ...
Expression and Purification of Recombinant Proteins That Have Native Amino
by Stratagene. 1 It is derived from the pET-11 vector series
and contains the lacI q gene for expression of the Lac repressor
protein and the hybrid T7 promoter for controlled expression of the inserted
protein coding sequence. 7,8 The vector is ...
High-Level Protein Expression, One-Column Purification, and FLAG
Epitope Tagging in E. coli
... released by
Stratagene. It is derived from the pET-11 vector series and contains the
lacI q gene for expression of the Lac repressor
and the hybrid T7 promoter for controlled expression
of the inserted protein coding sequence. 8 The vector is ...
Repressor in Biological Products
NovaBlue Glycerol Stock from Novagen
Description:... high yields of excellent quality plasmid DNA. The DE3 lysogen of NovaBlue is potentially useful as a stringent host due to the presence of the lacIq repressor
encoded by the F ....
Repressor in Biological Definition
... The lac repressor
is an enzyme which stops the metabolism of ... is scarce and lactose is plentiful the lac repressor
becomes inactive to permit the conversion of ... crank up production of a particular substance. A repressor
tells the cell to reduce production.
The first ...
... phage DNA is now part of the host's genome.
In the following, the convention that genes are ... protein encoded by that gene. The lambda repressor
gene system consists of (from left to right on ... OR3
The lambda repressor
is a dimer also known as the cI protein. It ...
... can also eventually lead to cancer. A mutation in one oncogen or one tumor repressor
gen is usually not enough for a tumor to occur. A combination of a number ...
DNA microarrays can be used to determine if oncogens or tumor repressor
gens have been mutated. Possibly in the future tumors can be treated ...
... a repressor.
The "operator" is a DNA binding site for a repressor;
transcription of the structural genes takes place unless a repressor
is bound to the operator.
gene regulatory network
Repressor in Biological Dictionary
... A repressor
is a DNA-binding protein that regulates the ... of expression is called repression. The lac repressor
is a DNA-binding protein which inhibits the ... bacterium ... The lac operon and its repressor
illustrate the effects of negative regulation ......
... The lac repressor
is a DNA-binding protein which inhibits the ... a terminator, and an ... Lac repressor
Binding to DNA. for the Atlas of Macromolecules in Protein Explorer ... 1OSL: Lac repressor
bound to nonspecific DNA 18-mer. ... Lac ...
... including an ... The operon may also contain regulatory genes such as a repressor
gene which ... s, are not necessarily coded for by that operon . ... The operon may also contain regulatory genes such as a repressor. repressor
... Operon gene regulation ... Molecular Cell Biology concentrates ...
... is a virus particle consisting of a head, containing double ... The repressor
found in the phage lambda is a notable example of the level of ...... is a virus particle consisting of a head, containing double ... The repressor
found in the phage lambda is a notable example of the level of ... ...
... Bioinformatics Institute ... Helix - turn - helix biotin operon repressor
region. Example proteins. Open in usermanual ... Notably there is a ... motif consists of two a helices and a short extended amino ... Each l repressor
contains a helix - turn - helix motif. ... What does HTH stand for? ...
... Finding Zinc Fingers that Bind ... Zinc fingers are a common protein motif ... A particular six zinc finger combination fused with a repressor
should silence ... ...
... perinuclear, and pachytene B, when SCs are uniformly distributed ... We find that the pachytene checkpoint requires Sum1, a transcriptional repressor
... NDT80 is required for exit from pachytene (Xu et al., 1995) ... Staging of pachytene nuclei based on the morphology of the XY pair with ...
... Library :: G1 Phase :: A fine-structure map of spontaneous mitotic ... Exit from G1 and S phase of the cell cycle is regulated by repressor
complexes ... G1 phase • In the cell cycle, the gap between the end of mitosis and the onset of the S phase . ... the multicellular ...
... orthologous genes that undergo exon skipping. ... For example, mutations in exon 18 of the breast cancer BRCA1 gene cause ... PTB: A repressor
OF EXON DEFINITION. MECHANISMS OF PTB REPRESSION ... The recognition of intron- exon boundaries, the splice sites, however, requires ... ...
... AP endonuclease (Ape1 or ref-1) DNA base excision repair (BER) enzyme ... Human AP - endonuclease 1 and hnRNP-L interact with a nCaRE-like repressor
element ... environmental health issues ... AP endonucleases from other species can replace Apn1 ... Robust class II AP endonuclease ...