Potential Cure for HIV Discovered
... he wrote. "Although favorable results would represent perhaps only a baby step toward eventual use in patients, the discovery of the Tre recombinase proves that enzymatic removal of integrated HIV-1 from human chromosomes is a current-day reality," he said. The researchers who developed ...Mammalian Expression Vectors with Interchangeable Selectable Markers
... pExchange core expression vector, drug-resistance modules and Cre recombinase protein. The pExchange core vectors are based on the pCMV-Script ... This reaction is followed by heat inactivation of Cre recombinase at 65C for 15 to 30 minutes. The inactivated recombination ...* Quick & Easy E. coli Gene Deletion Kit from Gene Bridges GmbH
Description:... The use of an FRT-flanked resistance cassette for the replacement of the targeted gene allows the subsequent removal of the selection marker by a Flp recombinase step, if required. Flp expression plasmids can be purchased from Gene Bridges. Multiple knockouts can be generated either by a repetitive insertion ...* Quick & Easy Conditional Knock Out Kit - FRT from Gene Bridges GmbH
Description:... promoter (PGK) for expression of neomycin resistance in mammalian cells. The included pCI-FLPe expression plasmid codes for an improved Flp recombinase which shows a four to tenfold higher recombinational activity compared to the wildtype Flp enzyme at temperatures between 37C (optimal growth ...