A gene's first 'kiss' sets off that affair known as puberty
...ng hormone (GnRH) is secreted and sets off a chain reaction of chemical messages. Inside the hypothalamus, nerve cells release GnRH in a 'round-the-clock,' pulsatile fashion. With each secretion, the pituitary gland is stimulated to secrete its own messengers, lutenizing hormone (LH) and follicle-stimulating ...Spleen may be source of versatile stem cells
...that a treatment designed to address theautoimmune reaction underlying type 1 diabetes actually cured thedisease in diabetic mice. Late in 2003 they reported the mechanismbehind the earlier discovery: cells from the spleens of donor mice -intended to train the diabetic animals' immune systems not to attackisl...Learning to fight an adversary that won't stay down
...ovations such as highly efficient polymerase chain reaction (PCR) have made it possible to identify and study the many genes responsible for antibiotic resistance in hospitals and the environment. "What has been found is that there are more antibiotic resistance genes around than we ever realized," says Dr. ...Gene therapy converts dead bone graft to new, living tissue
...ther scientists have attempted to invoke a similar reaction in dead bone by infusing tissue-growing proteins directly into the bone. This has proven successful for the small bone transplants used in spinal fusion, but not for larger grafts, because the proteins' effectiveness wear off in just hours. By contra......jected with cat allergen, GFD blocked the allergic reaction involving the human cell receptors, an indication that it might also work in people. Scientists made another set of mice allergic to cats by injecting them with cat protein and an immune system booster. These mice became “actively allergic?to cats: ...Cleland’s Reagent from Calbiochem
...S. Cyclizes as it reduces disulfides to thiols, so reaction is "driven" to completion. A protective agent for SH groups. Does not contain waxy surfactant found in many other preparations. Blocks the lethal and hypnotic effects of pentobarbitol. Purity: ≥97% by titration. Contaminants: Heavy metals: ≤...Cleland’s Reagent from Calbiochem
...S. Cyclizes as it reduces disulfides to thiols, so reaction is "driven" to completion. A protective agent for SH groups. Does not contain waxy surfactant found in many other preparations. Blocks the lethal and hypnotic effects of pentobarbitol. Purity: ≥97% by titration. Contaminants: Heavy metals: ≤...Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Placenta from OriGene Technologies
...the positive well(s) by gel electrophoresis of the reaction products, the second PCR analysis is carried out in the corresponding 96-well "Sub-Plate(s)," which contains all 5,000 clones from the positive well of the Master Plate arrayed at only 50 clones per well. Since this plate contains glycerol stocks of ...Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Heart from OriGene Technologies
...the positive well(s) by gel electrophoresis of the reaction products, the second PCR analysis is carried out in the corresponding 96-well "Sub-Plate(s)," which contains all 5,000 clones from the positive well of the Master Plate arrayed at only 50 clones per well. Since this plate contains glycerol stocks of ...Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Testis from OriGene Technologies
...the positive well(s) by gel electrophoresis of the reaction products, the second PCR analysis is carried out in the corresponding 96-well "Sub-Plate(s)," which contains all 5,000 clones from the positive well of the Master Plate arrayed at only 50 clones per well. Since this plate contains glycerol stocks of ...New Microarray Labeling Kit for Preparing cDNA Probes
...nverted into cDNAprobes in a reverse transcription reaction that incorporates labelednucleotides. The test and... and rbcL mRNA added to the probe-labeling reaction correspond to mRNA of high, medium, and low abundances, respectively. The relative fluoresce...Assess the In Vivo Activation of Signal Transduction Pathways with PathDetect Reporting Systems
...re dishes. The total amount of DNA in each reaction was kept constant by adding quantities as needed of an unrelated plasmid (pBluescript plasmid). The pFC2-dbd plasmid, which expresses the GAL4 DNA binding domain but lacks an activation domain, was used as the negative contr...Low Abundance cDNA Cloned Using Stratagenes Human Universal cDNA Library
... apo-B mRNA involves a site-specific deamination reaction that converts cytidine 6666 to uridine and generates a premature translational stop codon. 2 Although the catalytic subunit of the editing enzyme was discovered in 1993, the identity of the RNA-binding subunit has be...Automated Fluorescent-Tag Cycle Sequencing
... ABI PRISM dye terminator cycle sequencingready reaction kit. The new Hot Top Assembly eliminates the need ...automatically determine thecorrect sequence of the reaction template with greater than 98% accuracy. 2 PE Applied Biosystems have found that, for their ABI PR...Effects of Thermal Cycler Well-To-Well Temperature Uniformity on PCR Results
...ing patterns. When performing PCR amplifications, reaction components and PCR parametersare not the only crit...lowing thermal cycling, aliquots from each reaction were run on a vertical 6% polyacrylamide/TBE minigel, stained with ethidium bromide, and vis...PCR emerging as a promising technique for Diagnosing Urinary Tuberculosis
...nosis is made by exclusion. Urine polymerase chain reaction (PCR) is emerging as a promising technique for early diagnosis of Urinary Tuberculosis. The technique involves detection of bacteria by facilitating rapid amplification of small amounts of a genome sequence, Dr Narmada P Gupta told the Millennium Int...Sperm washing helps couples – Breakthrough in focu
...ified before insemination using a polymerase chain reaction nucleic acid-based sequence amplification assay. Pregnancy rate per insemination is 14%, based on a European experience of more than 2000 inseminations; to date, there have been no seroc...Patients often assume wrongly that they are allergic to penicillin
...to penicillin, based on a recollected history of a reaction to the antibiotic, were incorrect when assessed by skin allergy testing. Salkind said that only around 2% to 5% of the general population has a true allergy to penicillin. These allergic reactions, which can be measured by the presence of antibodies......his method into the patients body do not cause the reaction or turn malignant. The next step is to look for ways to produce more clotting factor consistently.......ress disorder (PTSD), a long-lasting psychological reaction that is more commonly linked with war and natural disaster. The problem is that these patients may go undetected ,become emotionally detached. Their heart rates after the operation are low, rather than elevated. It is very essential to treat PTSD at ...When Volume Matters, Immunoassay Systems Deliver
...rigeration promotes reagent stability. The devices reaction vessel loader provides capacity for up to three hours of continuous sample processing Integrated reagent packs contain all necessary assay-specific reagents in self-resealing bar-coded packs requiring no reagent preparation. Automatic onboard waste ...Prothrombin Time Comes in Small (CLIA-Waived) Packages
...ause pre-incorporated iron particles in the strips reaction chamber to move. An endpoint is reached when the formation of a clot causes immobilization of these particles. The company says that the system includes one of the fastest coagulation meters available, with results available within one minute. The ha...Rapid Flu Tests May Prove Especially Helpful This Season
... serology, rapid antigen testing, polymerase chain reaction (PCR) and immunofluorescence testing. All are time-consuming, however, in a year when time is especially important. Fortunately, three medical firms produce quick-results kits that have been CLIA-waived by the CDC for point-of-care use. With all of ...Point of Care Cardiac Analyzers Yield Rapid Cardiac Marker Results
... from plasma via filter, the plasma moves into the reaction chamber and reacts with fluorescent-conjugated antibodies to all three markers. After incubation, the sample-reagent mixture flows through the device detection lane where complexes of analyte and conjugates are captured. The level of fluorescence i...... disposable microcuvette, which serves as pipette, reaction vessel, and measuring cuvette. Approximately 5 l blood is drawn into the cuvette cavity by capillary action and spontaneously mixed with dried reagents on the inner walls. The cuvette is then placed into the analyzer, the absorbance is measured, an...