Navigation Links
Polynucleotide in Biological News

Stretching DNA to the limit

... is drastically reduced in width with increasing exposure to UV light. UV light induces the crosslinking of the constituent DNA bases within the polynucleotide chains, as well as causes the formation of lesions by linking together the adjacent strands. The small changes in structure induced by this ...
Polynucleotide in Biological Technology

Evaluation of FlashPlate in a Helicase Assay

... NaCl, 1 mM DTT, 0.5 mM MnC l 2 , 20% glycerol and 1 mM ATP) polynucleotide Kinase (T4) (NEE101) [ 33 P]-γ-ATP (NEG602H) ... 1. DNA oligo #2 was 5'-labeled with 33 P by T4 polynucleotide kinase, following the manufacturer's protocol. 2. Equal molar ...

USB OptiKinase Eliminates Base Bias in Labeling of Oligonucleotide 5'-Ends

... Wild-type T4 polynucleotide Kinase (PNK) (1,2,3) is commonly used for phosphorylation and labeling of 5'-ends of DNA and oligonucleotides. Labeling, in particular, can be ...

The Bio-Rad Silver Stain, Rev E

... vesicles, Proc Natl Acad Sci USA 82, 41374141 (1985) 33. Khan ZA and Fraenkel-Conrat H, Purification and characterization of polynucleotide phosphorylase from cucumber, Proc Natl Acad Sci USA 82, 13111315 (1985) 34. King SM et al., Characterization of monoclonal ...

Rapidly Prepare Probes and Markers for Small RNA Analysis

... Preparation of RNA markers and DNA or RNA probes starts with a phosphorylation reaction using T4 polynucleotide Kinase and Kinase Buffer supplied with the kit, and [gamma- 32 P]ATP. If required, Kinase Buffer, enzyme, ...

Highly Sensitive microRNA Array Performance

... oligonucleotides with sequences complementary to the known human, mouse, and rat miRNAs. The probes have a polynucleotide spacer that ensures maximal hybridization efficiency to labeled miRNAs when the probes are arrayed on ...

Human Universal cDNA Library Array I

... were performed in 6X SSC buffer, 5X Denhardts reagent, and 0.1% SDS. The oligonucleotide probes were prepared using T4 polynucleotide kinase and high-specific-activity [g- 32 P]ATP (3000 Ci/mmol). The specific activity for the probes were approximately 5 10 8 ...
Polynucleotide in Biological Products

T4 Polynucleotide Kinase Exchange Reaction Buffer from Invitrogen

Description:... For use with T4 polynucleotide Kinase for labeling of 5' ends (5' phosphate ends) of nucleic acids. In the exchange reaction T4 polynucleotide Kinase transfers the 5' phosphate group from DNA to ADP and subsequently ...
Company:Invitrogen

Calf Alkaline Phosphatase, Calf Intestinal, Unconjugated from Promega

Description:... both 5-termini and may also be used for the dephosphorylation of 5 phosphorylated ends of DNA or RNA for subsequent labeling with [32P]ATP and T4 polynucleotide Kinase. CIAP is active on 5 overhangs, 5 recessed and blunt ends ....
Company:Promega

T4 Polynucleotide Kinase from Promega

Description:... T4 polynucleotide Kinase catalyzes the transfer of the ?-phosphate from ATP to the 5-terminus of polynucleotides or to mononucleotides bearing a 5-hydroxyl group. The ...
Company:Promega

GeneAmp PCR Carry-over Prevention Kit from Applied Biosystems

Description:... DNA templates. Products from previous PCR amplifications are eliminated by excising uracil residues using UNG, and degrading the resulting abasic polynucleotide with heat. Although UNG is active on single- and double-stranded dU-containing DNA,2 ribouracil residues in RNA and dUTP itself are not substrates ...
Company:Applied Biosystems

RNA Polymers - Poly rA. 2 Poly rU Triple-Stranded Polymer, sodium salt from Midland Certified Reagent Company

Description:... Polyribonucleotides (RNA polymers) are prepared by the catalytic action of the enzyme, polynucleotide phosphorylase, isolated at Midland, and the appropriate, ribonucleoside diphosphate as substrate. With the exception of catalog number P-3102, RNA ...
Company:Midland Certified Reagent Company

T4 Polynucleotide Kinase from EPICENTRE Biotechnologies

Description:... T4 polynucleotide Kinase (PNK) catalyzes the transfer of the gamma-phosphate from ATP to the 5'-hydroxyl of single- and double-stranded DNA, RNA, and nucleoside ...
Company:EPICENTRE Biotechnologies
Polynucleotide in Biological Definition

DNA

... referred to by the identity of their bases. These are adenine (A), thymine (T), cytosine (C), and guanine (G). In a DNA double helix, two polynucleotide strands can associate through the hydrophobic effect . Specificity of which strands stay associated is determined by complementary pairing . Each ...
Polynucleotide in Biological Dictionary

Polynucleotide Kinase

... In enzymology, a polynucleotide 5'-hydroxyl- kinase (EC 2.7.1.78) is an ... that catalyzes the chemical reaction Use this polynucleotide kinase to: phosphorylate 5_ -ends of DNA or RNA ... T4 polynucleotide Kinase ; 3'-phosphatase-free. Alkaline ...

Phosphodiester Bond

... polymerase get its energy to make phosphodiester bond ? ... phosphodiester bond n. The covalent chemical bond that holds together the polynucleotide chains of RNA and DNA by joining a carbon in the pentose The phosphate groups in the phosphodiester bond are negatively-charged. ... A ...

Okazaki fragments

... singles, watch music ... Okazaki Fragments's Latest Blog Entry [Subscribe to this Blog] ... Details on Okazaki Fragments , DNA Polymerases, polynucleotide Ligases, Okazaki , Vitro Synthesis, Replication of DNA ... that Okazaki fragments are initiated ... The initiator RNAs of mammalian Okazaki ...

Kinase

... and important, but it is only one family in a ... Hence, the protein kinase -like superfamily has undergone substantial structural ... T4 polynucleotide Kinase catalyzes the transfer and exchange of P<sub>i</sub> from the γ position of ATP to the 5' -hydroxyl terminus of double- and ...

Exonuclease

... Exonucleases are enzymes that work by cleaving nucleotides one at a time from the end of a polynucleotide chain. A hydrolyzing reaction occurs that breaks phosphodiester bonds at either the 3’ or 5’ ends. Its close ... exonuclease ....

Concatemer

... utilises a concatemer of the target polynucleotide, i.e. repeating ... [0050]The concatemer may be formed in any suitable way. ... ... each polynucleotide comprises a concatemer having multiple copies of a target ... distributed on the surface in a manner to promote the fixing of one concatemer ... ...

Codon

... What is codon ? Meaning of codon medical term. What does codon mean? ... codon /co·don/ (ko'don) a series of three adjacent bases in one polynucleotide ... Codon . National Human Genome Research Institute (NHGRI) by artist Darryl Leja. Look up a description of Codon in the NHGRI Talking ...

Base sequence

... of the sequenced molecule. base sequence ( ′bās ′sēkwəns ) ( genetics ) The specific order of purine and pyrimidine bases in a polynucleotide such as deoxyribonucleic acid or sequence ( ) n. A following of one thing after another; succession. ... dna having the sequence ...
Other Contents
(Date:1/22/2015)... (PRWEB) January 22, 2015 EBSCO Information ... (AMA) are expanding their relationship in an effort to further ... the world. While EBSCO has long made AMA journals available ... sales agent for The JAMA Network. , Long ...
(Date:1/22/2015)... is a famous dress online store for wedding dresses and ... collection of wedding dresses , and launches a site-wide women’s ... dress on your big day; the wedding gown is the ... to find the most suitable wedding dress. Now, we are ...
(Date:1/22/2015)... 22, 2015 Lower-Auto-Insurance.com has released a new blog ... a car for reducing the costs of an auto insurance ... be able to get lower prices for their vehicle insurance policies. ... role in determining policy costs. Because of this, drivers should ...
(Date:1/22/2015)... NORCROSS, Ga., USA (PRWEB) January 22, 2015 ... implementing a statewide data management system with support from ... Germany. , The new solution will leverage Hexagon Geospatial’s ... raster and vector data as well as point clouds ...
(Date:12/26/2014)... It is said that nothing makes a lady feel more ... dress supplier, shows its new selection of navy cocktail ... , UWDress.com’s navy cocktail gowns are the cornerstones of the ... styles. Simple dresses in bright neon colors can be dressed ...
Breaking Medicine News(10 mins):Health News:EBSCO Expands Partnership with the American Medical Association 2Health News:EBSCO Expands Partnership with the American Medical Association 3Health News:EBSCO Expands Partnership with the American Medical Association 4Health News:Find The Most Suitable Wedding Dress At JJsHouse.com 2Health News:Driving Safe And Other Factors That Make Auto Insurance Affordable! 2Health News:State Forestry Institute Mecklenburg-Vorpommern, Germany Embraces Hexagon Geospatial Technology 2Health News:State Forestry Institute Mecklenburg-Vorpommern, Germany Embraces Hexagon Geospatial Technology 3Health News:UWDress.com Shows Its New Navy Cocktail Dresses For 2015 2
(Date:12/17/2014)... , Dec. 15, 2014 The Defense Logistics Agency ... detect and prevent counterfeit microcircuits from entering into its ... an in-house microcircuit anti-counterfeit initiative dubbed DNA marking. The ... increasing their reliability throughout the supply chain. The new ...
(Date:12/17/2014)... Dec. 16, 2014 Research and Markets ( ... "Global Chemical Sensor Market 2015-2019" report to their ... One major trend upcoming in this market is ... Chemical sensors help in recording of patient data for ...
(Date:12/11/2014)... , Dec. 09, 2014 Research and Markets ... ( http://www.researchandmarkets.com/research/twhjll/biometrics_market ) has announced the addition of ...  report to their offering. One major ... With continuous advances in technology, it is important to ...
Breaking Biology News(10 mins):Defense Logistics Agency Launches DNA Marking Capability to Strengthen Microcircuit Supply Chain 2Defense Logistics Agency Launches DNA Marking Capability to Strengthen Microcircuit Supply Chain 3Global Chemical Sensor Market 2015-2019: Key Vendors are Abbott Laboratories, Bayer, Hoffmann La-Roche, Johnson & Johnson, NGK Spark Plugs and Robert Bosch 2Biometrics Market in the APAC Region 2015-2019: Key Vendors are 3M Cogent, NEC, Safran and Suprema 2
Other TagsOther Tags