Navigation Links
Polynucleotide in Biological News

Stretching DNA to the limit

... is drastically reduced in width with increasing exposure to UV light. UV light induces the crosslinking of the constituent DNA bases within the polynucleotide chains, as well as causes the formation of lesions by linking together the adjacent strands. The small changes in structure induced by this ...
Polynucleotide in Biological Technology

Evaluation of FlashPlate in a Helicase Assay

... NaCl, 1 mM DTT, 0.5 mM MnC l 2 , 20% glycerol and 1 mM ATP) polynucleotide Kinase (T4) (NEE101) [ 33 P]-γ-ATP (NEG602H) ... 1. DNA oligo #2 was 5'-labeled with 33 P by T4 polynucleotide kinase, following the manufacturer's protocol. 2. Equal molar ...

USB OptiKinase Eliminates Base Bias in Labeling of Oligonucleotide 5'-Ends

... Wild-type T4 polynucleotide Kinase (PNK) (1,2,3) is commonly used for phosphorylation and labeling of 5'-ends of DNA and oligonucleotides. Labeling, in particular, can be ...

The Bio-Rad Silver Stain, Rev E

... vesicles, Proc Natl Acad Sci USA 82, 41374141 (1985) 33. Khan ZA and Fraenkel-Conrat H, Purification and characterization of polynucleotide phosphorylase from cucumber, Proc Natl Acad Sci USA 82, 13111315 (1985) 34. King SM et al., Characterization of monoclonal ...

Rapidly Prepare Probes and Markers for Small RNA Analysis

... Preparation of RNA markers and DNA or RNA probes starts with a phosphorylation reaction using T4 polynucleotide Kinase and Kinase Buffer supplied with the kit, and [gamma- 32 P]ATP. If required, Kinase Buffer, enzyme, ...

Highly Sensitive microRNA Array Performance

... oligonucleotides with sequences complementary to the known human, mouse, and rat miRNAs. The probes have a polynucleotide spacer that ensures maximal hybridization efficiency to labeled miRNAs when the probes are arrayed on ...

Human Universal cDNA Library Array I

... were performed in 6X SSC buffer, 5X Denhardts reagent, and 0.1% SDS. The oligonucleotide probes were prepared using T4 polynucleotide kinase and high-specific-activity [g- 32 P]ATP (3000 Ci/mmol). The specific activity for the probes were approximately 5 10 8 ...
Polynucleotide in Biological Products

T4 Polynucleotide Kinase Exchange Reaction Buffer from Invitrogen

Description:... For use with T4 polynucleotide Kinase for labeling of 5' ends (5' phosphate ends) of nucleic acids. In the exchange reaction T4 polynucleotide Kinase transfers the 5' phosphate group from DNA to ADP and subsequently ...
Company:Invitrogen

Calf Alkaline Phosphatase, Calf Intestinal, Unconjugated from Promega

Description:... both 5-termini and may also be used for the dephosphorylation of 5 phosphorylated ends of DNA or RNA for subsequent labeling with [32P]ATP and T4 polynucleotide Kinase. CIAP is active on 5 overhangs, 5 recessed and blunt ends ....
Company:Promega

T4 Polynucleotide Kinase from Promega

Description:... T4 polynucleotide Kinase catalyzes the transfer of the ?-phosphate from ATP to the 5-terminus of polynucleotides or to mononucleotides bearing a 5-hydroxyl group. The ...
Company:Promega

GeneAmp PCR Carry-over Prevention Kit from Applied Biosystems

Description:... DNA templates. Products from previous PCR amplifications are eliminated by excising uracil residues using UNG, and degrading the resulting abasic polynucleotide with heat. Although UNG is active on single- and double-stranded dU-containing DNA,2 ribouracil residues in RNA and dUTP itself are not substrates ...
Company:Applied Biosystems

RNA Polymers - Poly rA. 2 Poly rU Triple-Stranded Polymer, sodium salt from Midland Certified Reagent Company

Description:... Polyribonucleotides (RNA polymers) are prepared by the catalytic action of the enzyme, polynucleotide phosphorylase, isolated at Midland, and the appropriate, ribonucleoside diphosphate as substrate. With the exception of catalog number P-3102, RNA ...
Company:Midland Certified Reagent Company

T4 Polynucleotide Kinase from EPICENTRE Biotechnologies

Description:... T4 polynucleotide Kinase (PNK) catalyzes the transfer of the gamma-phosphate from ATP to the 5'-hydroxyl of single- and double-stranded DNA, RNA, and nucleoside ...
Company:EPICENTRE Biotechnologies
Polynucleotide in Biological Definition

DNA

... referred to by the identity of their bases. These are adenine (A), thymine (T), cytosine (C), and guanine (G). In a DNA double helix, two polynucleotide strands can associate through the hydrophobic effect . Specificity of which strands stay associated is determined by complementary pairing . Each ...
Polynucleotide in Biological Dictionary

Polynucleotide Kinase

... In enzymology, a polynucleotide 5'-hydroxyl- kinase (EC 2.7.1.78) is an ... that catalyzes the chemical reaction Use this polynucleotide kinase to: phosphorylate 5_ -ends of DNA or RNA ... T4 polynucleotide Kinase ; 3'-phosphatase-free. Alkaline ...

Phosphodiester Bond

... polymerase get its energy to make phosphodiester bond ? ... phosphodiester bond n. The covalent chemical bond that holds together the polynucleotide chains of RNA and DNA by joining a carbon in the pentose The phosphate groups in the phosphodiester bond are negatively-charged. ... A ...

Okazaki fragments

... singles, watch music ... Okazaki Fragments's Latest Blog Entry [Subscribe to this Blog] ... Details on Okazaki Fragments , DNA Polymerases, polynucleotide Ligases, Okazaki , Vitro Synthesis, Replication of DNA ... that Okazaki fragments are initiated ... The initiator RNAs of mammalian Okazaki ...

Kinase

... and important, but it is only one family in a ... Hence, the protein kinase -like superfamily has undergone substantial structural ... T4 polynucleotide Kinase catalyzes the transfer and exchange of P<sub>i</sub> from the γ position of ATP to the 5' -hydroxyl terminus of double- and ...

Exonuclease

... Exonucleases are enzymes that work by cleaving nucleotides one at a time from the end of a polynucleotide chain. A hydrolyzing reaction occurs that breaks phosphodiester bonds at either the 3’ or 5’ ends. Its close ... exonuclease ....

Concatemer

... utilises a concatemer of the target polynucleotide, i.e. repeating ... [0050]The concatemer may be formed in any suitable way. ... ... each polynucleotide comprises a concatemer having multiple copies of a target ... distributed on the surface in a manner to promote the fixing of one concatemer ... ...

Codon

... What is codon ? Meaning of codon medical term. What does codon mean? ... codon /co·don/ (ko'don) a series of three adjacent bases in one polynucleotide ... Codon . National Human Genome Research Institute (NHGRI) by artist Darryl Leja. Look up a description of Codon in the NHGRI Talking ...

Base sequence

... of the sequenced molecule. base sequence ( ′bās ′sēkwəns ) ( genetics ) The specific order of purine and pyrimidine bases in a polynucleotide such as deoxyribonucleic acid or sequence ( ) n. A following of one thing after another; succession. ... dna having the sequence ...
Other Contents
(Date:5/29/2015)... 29, 2015 The Global Allopurinol ... study on the current state of the Allopurinol ... key statistics on the state of the industry ... direction for companies and individuals interested in the ... the industry including definitions, classifications, applications and industry ...
(Date:5/29/2015)... (PRWEB) May 29, 2015 Designed with ... teamed up to create an all new, exclusive line ... a formulated with the latest and greatest, high impact, ... proven to be effective. The 24/7 Series can be ... cross-fitters and even the casual fitness enthusiasts. There are ...
(Date:5/29/2015)... May 29, 2015 Javlin continues to ... U.S. headquarters and a larger team, with the intention ... CloverETL's market share in the U.S. and Canada. , ... Group, achieved significant growth in 2014 with its strongest ... premier data integration platform, CloverETL. To accelerate further progress ...
(Date:5/29/2015)... 2015 This is a professional ... the Naproxen industry.The report provides a basic overview ... industry chain structure. The Naproxen market ... development trends, competitive landscape analysis, and key regions ... well as manufacturing processes and cost structures are ...
(Date:5/29/2015)... Michigan (PRWEB) May 29, 2015 Vulvodynia ... and can cause significant physical, sexual, and psychological distress. ... altering disorder of Vulvodynia. The actual diagnosis is often ... in Endometriosis, they realize that this problem can be ... experiencing any of these symptoms, they need to schedule ...
Breaking Medicine News(10 mins):Health News:Allopurinol Industry (Global, China) 2015 Research Analysis and 2020 Forecasts 2Health News:BPI Sports and Bodybuilding.com Partner Up to Bring an Innovative New Line of Supplements 2Health News:BPI Sports and Bodybuilding.com Partner Up to Bring an Innovative New Line of Supplements 3Health News:Growth of Data Integration Platform CloverETL Continues in North America 2Health News:Naproxen Market Analysis For Global & China Regions 2020 Research Forecasts 2
(Date:5/11/2015)... Curemark LLC, a privately held drug research ... Phase III double blind, randomized, placebo-controlled clinical trial to ... all children ages 3-8 with Autism. Previously, Curemark announced ... blinded clinical trial for CM-AT in children ages 3-8 ... enzyme chymotrypsin. This new trial will help determine whether ...
(Date:5/5/2015)... , May 5, 2015, NXT-ID, Inc. (NASDAQ: ... company focused on the growing mobile commerce market, reminds investors ... Chief Technology Officer, is presenting  at CARTES SECURE CONNEXIONS AMERICA ... 5-7, 2015. The three-day conference is organized ... is speaking in is themed Global Fraud: Where is ...
(Date:4/20/2015)... Calif. , April 20, 2015 Huntington ... Gabriel Valley to implant a new miniaturized, wireless monitoring ... System is the first and only FDA-approved heart failure ... hospital admissions when used by physicians to manage heart ... sensor that is implanted in the pulmonary artery (PA) ...
Breaking Biology News(10 mins):Curemark, LLC, Launches New Phase III Trial in Expanded Population of Children with Autism 2NXT-ID, Inc.'s CTO, David Tunnell, Presents at CARTES SECURE CONNEXIONS AMERICA 2015 Today in Washington DC. 2NXT-ID, Inc.'s CTO, David Tunnell, Presents at CARTES SECURE CONNEXIONS AMERICA 2015 Today in Washington DC. 3Huntington Memorial Hospital Becomes First in the San Gabriel Valley to Offer Patients a New Heart Failure Monitoring Solution 2Huntington Memorial Hospital Becomes First in the San Gabriel Valley to Offer Patients a New Heart Failure Monitoring Solution 3
Other TagsOther Tags