Navigation Links
Polyadenylation in Medical Dictionary

Thalassemia

... from the nucleus to the cytoplasm, and in the control of mRNA stability. polyadenylation is a key regulatory step in the expression of many genes . Aberrant polyadenylation has been shown to cause disease s such as thalassemia ... ...
Polyadenylation in Biological News

Examination of internal 'wiring' of yeast, worm, and fly reveals conserved circuits

... conservation and overlap in yeast, worm, and fly in 71 distinct "network regions" that included such essential functions as protein degradation, RNA polyadenylation and splicing, protein phosphorylation and signal transduction, DNA synthesis, nuclear-cytoplasmic transport, and protein folding. Such networks ...
Polyadenylation in Biological Technology

Cis-Reporting System Monitors Intracellular Calcium Mobilization

... widely used NFAT binding sequence. 1,2,10,11 In addition to a polyadenylation signal downstream of the luciferase gene, the pNFAT-Luc plasmid contains another polyadenylation signal in front of the NFAT binding sites and TATA box. This ...

Cis-Reporting System Monitors Intracellular Calcium Mobilization

... widely used NFAT binding sequence. 1,2,10,11 In addition to a polyadenylation signal downstream of the luciferase gene, the pNFAT-Luc plasmid contains another polyadenylation signal in front of the NFAT binding sites and TATA box. This ...

pSilencer 4.1-CMV: Versatile Vectors for Expression of siRNA, miRNA, and mRNA

... RNA polymerase II-type CMV promoter (human cytomegalovirus immediate-early promoter) and an optimized SV40 polyadenylation signal to drive high-level expression of hairpin siRNA. For stable transfections requiring long-term ...

Mammalian Expression Vector for Efficient Cloning of PCR Fragments

... efficiency and 1-hour ligation. However, the pCMV-Script vector has many distinguishing features. The powerful CMV promoter and the SV40 polyadenylation site allow high-level constitutive expression in a wide variety of cell lines. In addition, the neomycin-resistance gene is ...

Mammalian and Bacterial Expression in One Vector

... vectors. Yet mammalian and bacterial plasmid vectors vary considerably in their choice of promoters, the presence of splice signals and polyadenylation sites, as well as their respective translation initiation sequences. In fact, the differences between bacterial and mammalian expression vectors ...

High-Titer Retroviral Vectors for Gene Delivery

... region contains the retroviral promoter/enhancer, and is flanked in the 3 direction by the R region, which contains the viral polyadenylation signal (pA). The R region is followed by the U5 region, which, along with R, contains sequences that are critical for reverse ...

A New Lambda Vector for Mammalian Expression

... 6 gene expression driven by the CMV promoter for constitutive expression in a wide variety of cell lines; ### the SV40 polyadenylation site; and the neomycin-resistance gene under control of the prokaryotic b -lactamase promoter, to provide kanamycin ...

Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System

... with a shuttle vector. Fig.2 The pShuttle-CMV vector contains a multiple cloning site between the CMV promoter and the SV40 polyadenylation signal. The pShuttle vector contains only a multiple cloning site in which an entire expression cassette can be inserted. The regions indicated ...

New Mammalian Two-Hybrid System Detects Protein-Protein Interactions

... the pCMV-BD and pCMV-AD vectors. The SV40 poly (A) signal provides the signals necessary for transcriptional termination and polyadenylation of the bait and target genes in mammalian cells. Stratagene offers this system as a vector kit containing both the bait vector, target (prey) ...

More>>

Polyadenylation in Biological Products

loxP flanked Ampicillin Selection Cassette (loxP-amp-loxP) from Gene Bridges GmbH

Description:... is a slightly modified version of the Em7 promoter. It mediates higher transcription efficiency than the generally used Tn5 promoter. A synthetic polyadenylation signal terminates the ampicillin expression. The cassette is flanked by lox sites for later excision by Cre recombinase. Unique Not I and Xho I ...
Company:Gene Bridges GmbH

FRT flanked Ampicillin Selection Cassette (FRT-amp-FRT) from Gene Bridges GmbH

Description:... is a slightly modified version of the Em7 promoter. It mediates higher transcription efficiency than the generally used Tn5 promoter. A synthetic polyadenylation signal terminates the ampicillin expression. The cassette is flanked by FRT sites for later excision by Flp recombinase. Unique Not I and Xho I ...
Company:Gene Bridges GmbH

loxP flanked Chloramphenicol Selection Cassette (loxP-cm-loxP) from Gene Bridges GmbH

Description:... is a slightly modified version of the Em7 promoter. It mediates higher transcription efficiency than the generally used Tn5 promoter. A synthetic polyadenylation signal terminates the chloramphenicol expression. The cassette is flanked by loxP sites for later excision by Cre recombinase. Unique Not I and Xho ...
Company:Gene Bridges GmbH

FRT flanked, Pro and Euk. Neomycin Selection Cassette plus loxP site(FRT-PGK-gb2-neo-FRT-loxP) from Gene Bridges GmbH

Description:... than the generally used Tn5 promoter. The promoter of the mouse Phosphoglucokinase gene (PGK) is used as the eukaryotic promoter. A synthetic polyadenylation signal terminates the kanamycin/neomycin expression. The cassette is flanked by FRT sites for later excision by Flp recombinase. An additional ...
Company:Gene Bridges GmbH

loxP flanked, Pro and Euk. Neomycin Selection Cassette (loxP-PGK-gb2-neo-loxP) from Gene Bridges GmbH

Description:... efficiency than the normally used Tn5 promoter. The promoter of the mouse Phosphoglucokinase gene (PGK) is used as eukaryotic promoter. A synthetic polyadenylation signal terminates the kanamycin/neomycin expression. The cassette is flanked by loxP sites for later excision by Cre recombinase. Unique Not I and ...
Company:Gene Bridges GmbH

FRT flanked, Pro and Euk. Neomycin Selection Cassette (FRT-PGK-gb2-neo-FRT) from Gene Bridges GmbH

Description:... efficiency than the normally used Tn5 promoter. The promoter of the mouse Phosphoglucokinase gene (PGK) is used as eukaryotic promoter. A synthetic polyadenylation signal terminates the kanamycin/neomycin expression. The cassette is flanked by FRT sites for later excision by Flp recombinase. Unique Not I and ...
Company:Gene Bridges GmbH

More>>

Polyadenylation in Biological Definition

Alternative splicing

... sites : this is the only method of splicing which can produce an alternative C-terminus domain in proteins. In this case, different sets of polyadenylation sites can be spliced with the other exons. Intron retaining mode : in this case, instead of splicing out an intron, the intron is retained in ...
Polyadenylation in Biological Dictionary

Polyadenylation

... polyadenylation is the addition of a poly(A) tail, a stretch of ... adenines, onto an RNA molecule. In eukaryotes, polyadenylation is part of the process that produces mature messenger RNA (mRNA) ... Cleavage and polyadenylation specificity factor (CPSF) is involved in the ...

Untranslated RNA

... 3 RNA ... uracil is appropriate for RNA , where quantity is important but ... RNA is created in the transcription; after splicing and polyadenylation it is ... Untranslated regions ... DNA strand that the RNA was transcribed from. ... ...

Pre-mRNA

... at an early stage of spliceosome assembly. ... ... the structure is a good mimic of the phosphate group of the pre - mRNA substrate. ... polyadenylation factor CPSF-73 is the pre - mRNA 3'-end-processing endonuclease. ... Pre mRNA information including symptoms, causes, diseases, symptoms, ...

PolyA tail

... polyadenylation is the addition of a poly(A ) tail , a stretch of RNA where all ... The poly(A ) tail is important for the nuclear export, translation and ...

MRNA processing

... post-transcriptional processing is far from ... ... processing events include cleavage at a specific site in the pre- mRNA followed ... polyadenylation factor CPSF-73 is the pre- mRNA 3'-end- processing endonuclease. ... mRNA ... PRP18 pre- mRNA processing factor 18 homolog (S. cerevisiae) ...

More>>

Other Contents
(Date:8/30/2014)... 30, 2014 Ticket Down reports that ... exhibition match next Saturday at Levi's Stadium. The summer ... fans in the United States due to the fact that so ... every major city. Many of these games were practice games ... past summer. , Levi’s Stadium is a brand new facility that ...
(Date:8/30/2014)... Dayton, OH (PRWEB) August 30, 2014 United ... school’s first fully online degree, the Master of Theological Studies ... completed in 2-3 years, is designed for students who wish ... deepen their understanding and capacity to serve the Church and ... this degree, but now they can attend classes from any ...
(Date:8/30/2014)... Patients suffering from vitiligo can now ... new review published by Daily Gossip. , Vitiligo ... there is no wonder that numerous sufferers search for ... Treatment is one of the most appreciated such methods ... to stop the spread of this disorder immediately, while ...
(Date:8/30/2014)... Daily Gossip magazine reveals in its "Panic ... attacks under control with a simple and efficient method. ... this new method as an anxiety recovery program which ... general anxiety related disorders. , What makes this ... it can be accessed by users from the comfort ...
(Date:8/30/2014)... The Plastic Surgery Group (PSG) is ... even in the U.S. to provide clients with Vanquish, ... patients’ waists with deep-tissue heating. , Unlike Cryotherapy which ... is more inclusive to target large sections of desired ... panels about one inch away from their body while ...
Breaking Medicine News(10 mins):Health News:Mexico vs. Chile Tickets at Levi's Stadium in Santa Clara, CA: TicketDown.com Offers Promo Code for Mexico vs. Chile Exhibition Game on September 6th 2Health News:United Theological Seminary Announces First Fully Online Degree 2Health News:Natural Vitiligo Treatment Review Exposes Fast Method for Skin Repigmentation 2Health News:Panic Away Review Reveals New Natural Remedy for Anxiety Patients 2Health News:NJ Top Docs Presents, The Plastic Surgery Group 2
(Date:8/28/2014)... of America (ESA) is pleased to announce the selection ... the Society. Honorary Membership acknowledges those who have served ... in the affairs of the Society that has reached ... by the ESA Governing Board and then voted on ... at the Awards Ceremony at Entomology 2014, ESA,s Annual ...
(Date:8/28/2014)... . The zebrafish, ... striking pattern of blue stripes alternating with golden stripes. Three ... yellow cells emerge during growth in the skin of the ... compose the characteristic colour pattern. ,While it was ... form proper stripes, the embryonic origin of the pigment cells ...
(Date:8/28/2014)... ROCHESTER, Minn. A Mayo Clinic researcher and his ... speed up and enhance the process of re-engineering cells ... that uses network biology methods to aid stem cell ... cell engineering are described in two back-to-back papers in ... has a broad range of uses for all types ...
Breaking Biology News(10 mins):Fred Baxendale selected as Honorary Member of the Entomological Society of America 2How the zebrafish gets its stripes 2How the zebrafish gets its stripes 3New tool aids stem cell engineering for medical research 2
Other TagsOther Tags