Navigation Links
Polyadenylation in Medical Dictionary

Thalassemia

... from the nucleus to the cytoplasm, and in the control of mRNA stability. polyadenylation is a key regulatory step in the expression of many genes . Aberrant polyadenylation has been shown to cause disease s such as thalassemia ... ...
Polyadenylation in Biological News

Examination of internal 'wiring' of yeast, worm, and fly reveals conserved circuits

... conservation and overlap in yeast, worm, and fly in 71 distinct "network regions" that included such essential functions as protein degradation, RNA polyadenylation and splicing, protein phosphorylation and signal transduction, DNA synthesis, nuclear-cytoplasmic transport, and protein folding. Such networks ...
Polyadenylation in Biological Technology

Cis-Reporting System Monitors Intracellular Calcium Mobilization

... widely used NFAT binding sequence. 1,2,10,11 In addition to a polyadenylation signal downstream of the luciferase gene, the pNFAT-Luc plasmid contains another polyadenylation signal in front of the NFAT binding sites and TATA box. This ...

Cis-Reporting System Monitors Intracellular Calcium Mobilization

... widely used NFAT binding sequence. 1,2,10,11 In addition to a polyadenylation signal downstream of the luciferase gene, the pNFAT-Luc plasmid contains another polyadenylation signal in front of the NFAT binding sites and TATA box. This ...

pSilencer 4.1-CMV: Versatile Vectors for Expression of siRNA, miRNA, and mRNA

... RNA polymerase II-type CMV promoter (human cytomegalovirus immediate-early promoter) and an optimized SV40 polyadenylation signal to drive high-level expression of hairpin siRNA. For stable transfections requiring long-term ...

Mammalian Expression Vector for Efficient Cloning of PCR Fragments

... efficiency and 1-hour ligation. However, the pCMV-Script vector has many distinguishing features. The powerful CMV promoter and the SV40 polyadenylation site allow high-level constitutive expression in a wide variety of cell lines. In addition, the neomycin-resistance gene is ...

Mammalian and Bacterial Expression in One Vector

... vectors. Yet mammalian and bacterial plasmid vectors vary considerably in their choice of promoters, the presence of splice signals and polyadenylation sites, as well as their respective translation initiation sequences. In fact, the differences between bacterial and mammalian expression vectors ...

High-Titer Retroviral Vectors for Gene Delivery

... region contains the retroviral promoter/enhancer, and is flanked in the 3 direction by the R region, which contains the viral polyadenylation signal (pA). The R region is followed by the U5 region, which, along with R, contains sequences that are critical for reverse ...

A New Lambda Vector for Mammalian Expression

... 6 gene expression driven by the CMV promoter for constitutive expression in a wide variety of cell lines; ### the SV40 polyadenylation site; and the neomycin-resistance gene under control of the prokaryotic b -lactamase promoter, to provide kanamycin ...

Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System

... with a shuttle vector. Fig.2 The pShuttle-CMV vector contains a multiple cloning site between the CMV promoter and the SV40 polyadenylation signal. The pShuttle vector contains only a multiple cloning site in which an entire expression cassette can be inserted. The regions indicated ...

New Mammalian Two-Hybrid System Detects Protein-Protein Interactions

... the pCMV-BD and pCMV-AD vectors. The SV40 poly (A) signal provides the signals necessary for transcriptional termination and polyadenylation of the bait and target genes in mammalian cells. Stratagene offers this system as a vector kit containing both the bait vector, target (prey) ...

More>>

Polyadenylation in Biological Products

loxP flanked Ampicillin Selection Cassette (loxP-amp-loxP) from Gene Bridges GmbH

Description:... is a slightly modified version of the Em7 promoter. It mediates higher transcription efficiency than the generally used Tn5 promoter. A synthetic polyadenylation signal terminates the ampicillin expression. The cassette is flanked by lox sites for later excision by Cre recombinase. Unique Not I and Xho I ...
Company:Gene Bridges GmbH

FRT flanked Ampicillin Selection Cassette (FRT-amp-FRT) from Gene Bridges GmbH

Description:... is a slightly modified version of the Em7 promoter. It mediates higher transcription efficiency than the generally used Tn5 promoter. A synthetic polyadenylation signal terminates the ampicillin expression. The cassette is flanked by FRT sites for later excision by Flp recombinase. Unique Not I and Xho I ...
Company:Gene Bridges GmbH

loxP flanked Chloramphenicol Selection Cassette (loxP-cm-loxP) from Gene Bridges GmbH

Description:... is a slightly modified version of the Em7 promoter. It mediates higher transcription efficiency than the generally used Tn5 promoter. A synthetic polyadenylation signal terminates the chloramphenicol expression. The cassette is flanked by loxP sites for later excision by Cre recombinase. Unique Not I and Xho ...
Company:Gene Bridges GmbH

FRT flanked, Pro and Euk. Neomycin Selection Cassette plus loxP site(FRT-PGK-gb2-neo-FRT-loxP) from Gene Bridges GmbH

Description:... than the generally used Tn5 promoter. The promoter of the mouse Phosphoglucokinase gene (PGK) is used as the eukaryotic promoter. A synthetic polyadenylation signal terminates the kanamycin/neomycin expression. The cassette is flanked by FRT sites for later excision by Flp recombinase. An additional ...
Company:Gene Bridges GmbH

loxP flanked, Pro and Euk. Neomycin Selection Cassette (loxP-PGK-gb2-neo-loxP) from Gene Bridges GmbH

Description:... efficiency than the normally used Tn5 promoter. The promoter of the mouse Phosphoglucokinase gene (PGK) is used as eukaryotic promoter. A synthetic polyadenylation signal terminates the kanamycin/neomycin expression. The cassette is flanked by loxP sites for later excision by Cre recombinase. Unique Not I and ...
Company:Gene Bridges GmbH

FRT flanked, Pro and Euk. Neomycin Selection Cassette (FRT-PGK-gb2-neo-FRT) from Gene Bridges GmbH

Description:... efficiency than the normally used Tn5 promoter. The promoter of the mouse Phosphoglucokinase gene (PGK) is used as eukaryotic promoter. A synthetic polyadenylation signal terminates the kanamycin/neomycin expression. The cassette is flanked by FRT sites for later excision by Flp recombinase. Unique Not I and ...
Company:Gene Bridges GmbH

More>>

Polyadenylation in Biological Definition

Alternative splicing

... sites : this is the only method of splicing which can produce an alternative C-terminus domain in proteins. In this case, different sets of polyadenylation sites can be spliced with the other exons. Intron retaining mode : in this case, instead of splicing out an intron, the intron is retained in ...
Polyadenylation in Biological Dictionary

Polyadenylation

... polyadenylation is the addition of a poly(A) tail, a stretch of ... adenines, onto an RNA molecule. In eukaryotes, polyadenylation is part of the process that produces mature messenger RNA (mRNA) ... Cleavage and polyadenylation specificity factor (CPSF) is involved in the ...

Untranslated RNA

... 3 RNA ... uracil is appropriate for RNA , where quantity is important but ... RNA is created in the transcription; after splicing and polyadenylation it is ... Untranslated regions ... DNA strand that the RNA was transcribed from. ... ...

Pre-mRNA

... at an early stage of spliceosome assembly. ... ... the structure is a good mimic of the phosphate group of the pre - mRNA substrate. ... polyadenylation factor CPSF-73 is the pre - mRNA 3'-end-processing endonuclease. ... Pre mRNA information including symptoms, causes, diseases, symptoms, ...

PolyA tail

... polyadenylation is the addition of a poly(A ) tail , a stretch of RNA where all ... The poly(A ) tail is important for the nuclear export, translation and ...

MRNA processing

... post-transcriptional processing is far from ... ... processing events include cleavage at a specific site in the pre- mRNA followed ... polyadenylation factor CPSF-73 is the pre- mRNA 3'-end- processing endonuclease. ... mRNA ... PRP18 pre- mRNA processing factor 18 homolog (S. cerevisiae) ...

More>>

Other Contents
(Date:10/20/2014)... National Teen Driver Safety Week is October 19-25, ... Pediatrics (Ohio AAP, http://www.ohioaap.org ) is joining the National ... drivers to talk to their teens and always set the ... are the leading cause of death for U.S. teens. In ... and 859 (42%) of those teen drivers were killed in ...
(Date:10/20/2014)... It’s hard to imagine what it’s like to live without ... world, it’s a reality. And while new shoes are considered ... walk barefoot on rocky terrain or sewage-lined streets just to ... , Buckner International's Shoes for Orphan Souls ... to encourage support for Air1 and while also providing new ...
(Date:10/20/2014)... Silver Spring, Maryland, USA (PRWEB) October 20, 2014 ... readers or to track interventions? Here comes a wonderful ... leading mobile app development company helped its ... track Intervention more efficiently. The app is designed for ... iPad, and iPod touch. This app is optimized for ...
(Date:10/19/2014)... October 20, 2014 Recently, BambooIndustry.com, one ... unveiled its new range of bamboo deckings . ... these natural items; they are now available at deeply ... people, it is hard to overstate the significance of ... attention to online services. The company’s workers are striving ...
(Date:10/19/2014)... (PRWEB) October 20, 2014 Having been working ... a good knowledge of customer needs. According to James, one ... covered with dazzling sequins, or with high-low skirts that add ... a huge demand. Recently, LunaDress has unveiled its new selection ... of fact, the company’s brand new items are available in ...
Breaking Medicine News(10 mins):Health News:Ohio AAP Promotes National Teen Driver Safety Week 2Health News:Buckner Shoes for Orphan Souls® Garners Support from Air1 Radio 2Health News:Buckner Shoes for Orphan Souls® Garners Support from Air1 Radio 3Health News:Metassessor App Launched: an RTI tool for Teachers & Parents 2Health News:Metassessor App Launched: an RTI tool for Teachers & Parents 3Health News:High Quality Bamboo Deckings, New Items Unveiled By BambooIndustry.com 2Health News:2014 Long Prom Dresses From LunaDress Now Available In Over 20 Popular Designs 2
(Date:10/16/2014)... researchers have challenged conventional thinking on how the bowel ... mechanism for how bowel cancer starts. , The researchers ... and regenerating the ,crypts, that are a feature of ... involved in bowel cancer development, a controversial finding as ... , Using 3D imaging technologies, Dr Chin Wee Tan ...
(Date:10/16/2014)... Syncytial Virus causes severe respiratory tract infections and ... year. Scientists at VIB and Ghent University have ... counteract this common virus infection. , Xavier ... that paves the way for the development of ... virus that causes suffering in numerous small children ...
(Date:10/16/2014)... 16, 2014 NXT-ID, Inc. (NASDAQ: ... biometric authentication company focused on the growing mobile commerce ... including Wocket™, the Smart Wallet, at the 13 th ... a leading global conference on the intersection of technology ... Mark Anderson , founder and publisher of the Strategic ...
Breaking Biology News(10 mins):Cryptic clues drive new theory of bowel cancer development 2New perspectives for development of an RSV vaccine 2NXT-ID Inc. to Present at 13th Annual FiRe Conference 2NXT-ID Inc. to Present at 13th Annual FiRe Conference 3
Other TagsOther Tags