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Plasmid in Medical News

Bacterial 'battle for survival' leads to new antibiotic

... Streptomyces. A logical conclusion is that the plasmid carries the gene for rhodostreptomycin, but the researchers have sequenced more than half of the plasmid and found no genes that correlate to the antibiotic. Another theory is that the plasmid itself served as the insult that provoked ...

Researchers solve first structure of a key to intact DNA inheritance

... and often lethal staph infection. "The plasmid segregation system we are working on, called ... and segregation, Schumacher says, because plasmid segregation is relatively simple: two proteins ... grow, literally pushing the two replicated plasmid segrosomes apart, resulting in two copies of ...

Sangamo BioSciences Presents Positive Phase 2 ZFP Therapeutic Data at ADA 2009

... in previous pivotal large scale trials of other non-analgesic approaches for diabetic neuropathy." About SB-509 SB-509 is an injectable plasmid encoding a DNA-binding Zinc Finger DNA-binding Protein (ZFP) Transcription Factor (ZFP TF(TM)) designed to upregulate the endogenous expression of ...

Five UCSF Scientists Elected to National Academy of Sciences

... tolerance-inducing cells in the thymus that express the beta-cell specific insulin gene. As a graduate student, Hanahan developed high efficiency plasmid transformation methods and E. coli strains that have facilitated DNA cloning procedures; his work on E. coli transformation (the production of ...

iZumi Bio and Kyoto University Form Collaboration to Advance Induced Pluripotent Stem Cell Technology

... 2008, Dr. Yamanaka's group reported for the first time a virus-free method for inducing pluripotency in differentiated cells by using a pair of plasmid vectors to introduce the four iPS genes into embryonic mouse fibroblasts as part of CiRA's efforts to establish safe and efficient methods to ...

Senesco Announces Results of Pre-Clinical Efficacy, Toxicology and Dose Ranging Studies for Multiple Myeloma Therapeutic Candidate

... About SNS-01 Senesco's SNS-01 consists of three components: an eIF5A plasmid, an siRNA against eIF5A and polyethyleneiminie (PEI). The eIF5A plasmid upregulates the apoptotic pathways within cancer cells. The siRNA downregulates proinflammatory cytokines, NF-kB and ICAM, which are proliferation ...

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Plasmid in Medical Technology

Senesco Chooses VGXI to Produce Factor 5A Plasmid

... Inc. (The Woodlands, TX) ("VGXI") to supply the plasmid portion of the Company's combination therapy ... in vivo results using its combination siRNA and plasmid against subcutaneous multiple myeloma tumors in immunodeficient mice. The Factor 5A plasmid being produced by VGXI, as Senesco's preclinical ...

Vical Advances RapidResponse(TM) DNA Vaccine Platform Under $6 Million Grant from NIH

... effective dose, head-to-head comparison against plasmid DNA vaccine, Vaxfectin(R) formulation ... includes delivery of linear DNA as well as plasmid DNA. Currently plasmid DNA vaccines, which are under development ...

Genetic Immunity to Present at 2008 BIO International Convention

... the DermaVir Patch, which includes HIV-specific plasmid DNA encoded antigens. About Genetic ... technology that includes disease-specific plasmid DNA encoded antigens. DermaPrep is the ... together make up the DermaVir Patch. The plasmid DNA within DermaVir Patch's ...

Vical Pandemic Influenza Vaccines Achieve T-Cell Responses and Cross-Clade Reactivity in Humans

... responses through Day 182. Vical's monovalent vaccine contained a plasmid (a closed loop of DNA) encoding the hemagglutinin (HA) surface protein ... responses against H5 as well. Vical's trivalent vaccine contains the H5 plasmid plus separate plasmids encoding consensus sequences of two highly ...

Vical Announces Breakthrough for Pandemic Influenza DNA Vaccines With Preliminary Human Data

... faster development and production. Vical's monovalent vaccine contains a plasmid (a closed loop of DNA) encoding the hemagglutinin (HA) surface protein ... responses against H5 as well. Vical's trivalent vaccine contains the H5 plasmid plus separate plasmids encoding consensus sequences of two highly ...

Genetic Immunity Announces Data Related to the DermaVir Nanomedicine Patch in the Journal of Immunology

... is a patented nanoformulation technology that includes disease-specific plasmid DNA encoded antigens. DermaPrep is the topical administration device that ... of diseases. These two components together make up the DermaVir Patch. The plasmid DNA within DermaVir Patch's NanoComp nanoparticles is specific to HIV and ...

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Plasmid in Medical Products

E-Gel®96 High-Throughput Agarose Electrophoresis System

Description:... into an automated, high-throughput operation with the E-Gel 96 system. This bufferless, pre-cast system is ideal for analyzing multiple PCR products, plasmid preparations, restriction digests, and more. Fully automated, robot-compatible, and ready to put into action, the E-Gel 96 system makes your ...
Company:Invitrogen

hGH ELISA

Description:... hGH ELISA is used to quantitatively measure the expression of hGH released into the cell culture supernatant of eukaryotic cells transfected with a plasmid containing a reporter gene encoding for hGH. hGH Elisa is standardized, highly sensitive, specific, fast, no leaching, comparable, easy to perform ...
Company:Roche Diagnostics

QIAvac 24 Plus

Description:... of spin columns into and out of a centrifuge. The new QIAvac 24 Plus offers: Time savings rapid vacuum-driven purification and cleanup of plasmid or genomic DNA, PCR products, and RNA Convenience minimal hands-on-time Flexibility process up to 24 columns in parallel Versatility ...
Company:QIAGEN

Threshold Total System (110V)

Description:... (e.g., BSA, IgG, insulin, transferrin), Proteins A and G, and any unique protein that can be bound by antibodies as well as sequence-specific DNA and plasmid copy number. The system reduces the time required to develop, validate, and perform ultra-sensitive assays with maximum speed, sensitivity, ...
Company:Molecular Devices Corporation

Terrific Broth, Ready-Made Powder

Description:... Monobasic: 4% Storage: Ambient To Use: Dissolve 47 gm powder per liter of purified water. Autoclave. Application: Provides greater yields of plasmid DNA and recombinant proteins than LB media by allowing for greater cell ....
Company:USB Corporation

VersaFluor Cuvette Fluorometer

Features:... Changeable filters Is the only instrument needed to detect and quantitate DNA from PCR amplification, restriction digests, and plasmid preps, to quantitate apoptosis activity, and to assay the reporter enzymes beta-glucuronidase (GUS) and beta-galactosidase Quantitates DNA at ...
Company:Bio-Rad Laboratories

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Plasmid in Biological News

New insights into a leading poultry disease and its risks to human health

... animals and causing disease. Over time, each plasmid becomes a patchwork quilt of DNA information, ... database. Among the disease causing parts of the plasmid pAPEC-1 are a series of genes that make up ... bacteria. By analyzing the DNA sequence of plasmid pAPEC-1 and testing the mechanism of transfer of ...

Bioclocks work by controlling chromosome coiling

... occur naturally in the cyanobacteria. Because a plasmid should behave in the same fashion as the larger ... the behavior of the chromosome itself. When the plasmid is relaxed, it is open and uncoiled and, when it ... researchers found a distinct day/night cycle: The plasmid is smaller and more tightly wound during periods ...

New paradigm for cell-specific gene delivery

... cell-specific promoter that was able to mediate nuclear targeting of any plasmid carrying this sequence uniquely in cultured smooth muscle cells but in no ... delivery can be made cell-specific by controlling the nuclear entry of plasmid DNA, and as such, establishes a new paradigm for cell-selective gene ...

Nucleonics initiates hepatitis B clinical trial with expressed interfering RNA therapeutic

... ) is an expressed interfering RNA (eiRNA)-based product consisting of a plasmid DNA construct designed to produce four short interfering RNA (siRNA) ... thereby decreasing the chances of developing drug resistance. Moreover, plasmid DNA is non-immunogenic and a stable drug format that benefits from ...

Researchers observe single protein dimers wavering between two symmetrically opposed structures

... examined a protein known as Rop (repressor of primer), expressed in E. coli bacteria, which binds to RNA to regulate the number of copies of plasmid genes produced within the bacteria. This small "homodimer" is made up of two identical hairpin coils of amino acids, and has often been used as a ...

Another JDRF partner moves research forward with collaboration agreement for diabetes treatment

... for a beta cell regenerative therapy with Lilly. Bayhill's BHT-3021 compound is now in Phase I/II clinical trials supported by JDRF, is a plasmid encoding proinsulin designed to target specific pathogenic immune cells involved in the immune response of type 1 diabetes. ...

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Plasmid in Biological Technology

Senesco Signs a Supply Agreement for Polyplus-transfection's Delivery System

... therapy of siRNA against Factor 5A and a plasmid of the Factor 5A gene. Senesco has previously ... in vivo results using its combination siRNA and plasmid delivered with "in vivo-jetPEI" against ... delivery vehicle for a combination of siRNA and plasmid DNA in this therapeutic approach." About ...

Rentschler Biotechnologie Announces Cooperation with Boehringer Ingelheim

... proteins, protein scaffolds, monoclonal antibodies, antibody fragments and plasmid DNA. The Biberach site is specialized in highly efficient mammalian cell ... with exceptionally high productivities using proprietary systems. In the plasmid DNA manufacturing arena Boehringer Ingelheim in Austria has set the ...

Genetic Immunity To Present at the Rodman & Renshaw 5th Annual Global Healthcare Conference

... is a patented nanoformulation technology that includes disease-specific plasmid DNA encoded antigens. DermaPrep is the topical administration device that ... of diseases. These two components together make up the DermaVir Patch. The plasmid DNA within DermaVir Patch's NanoComp nanoparticles is specific to HIV and ...

EGEN, Inc. Announces the Promotion of Jason Fewell, Ph.D., to Vice-President of Preclinical Research and Development

... their novel compositions with nucleic acids and their therapeutic applications. EGEN's research pipeline includes products involving siRNA, shRNA and plasmid DNA for the treatment of various cancers and other diseases and has a number of collaborations with outside investigators, biotech organizations and ...

Frost & Sullivan Recognizes Cisbio Bioassays for Technology Innovation

... of one successful reaction during which it covalently links to its substrate. These substrates can be derivatized with HTRF fluorophores. A plasmid construct can be easily generated where the SNAP or CLIP tag is attached to the N-terminus of the GPCR. Thus, a fusion protein comprised of the ...

EGEN, Inc. Announces the Promotion of Khursheed Anwer, Ph.D., to President and Chief Scientific Officer

... their novel compositions with nucleic acids and their therapeutic applications. EGEN's research pipeline includes products involving siRNA, shRNA and plasmid DNA for the treatment of various cancers and other diseases and has a number of collaborations with outside investigators, biotech organizations and ...

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Plasmid in Biological Products

General DNA Sequencing Services from Polymorphic DNA Technologies, Inc.

Description:... Form and send it to info@polymorphicdna.com. plasmid DNA or PCR products should be purified by a ... but please do not provide samples in TE. plasmid DNA should be submitted at a concentration of 0.2 μg per μL. Please provide 2 μg of plasmid DNA per sequencing reaction requested. For PCR ...
Company:Polymorphic DNA Technologies, Inc.

Gene Construction Kit™ 2.5 from Textco, Inc.

Description:... KitTM allows graphic manipulation of DNA sequences and sophisticated plasmid drawing options. It has an award winning interface design that allows ... drawing capabilities for Mac and Windows users. GCK was named the "Best plasmid Mapping Software" by the Biotechnology Software and Internet Journal for ...
Company:Textco, Inc.

Express Cloning Checker Kit I from BioChain

Description:... recombinant colonies after transformation without time-consuming plasmid preparation. By using the large-scale screen method in Kit I, all you need ... spending two days using time and labor-intensive analysis methods such as plasmid DNA miniprep or colony hybridization. ... ...
Company:BioChain

Express Cloning Checker Kit II from BioChain

Description:... recombinant colonies after transformation without time-consuming plasmid preparation. By using the large-scale screen method in Kit I, all you need ... spending two days using time and labor-intensive analysis methods such as plasmid DNA miniprep or colony hybridization. ... ...
Company:BioChain

Express Cloning Checker Kit II: Green Solution from BioChain

Description:... recombinant colonies after transformation without time-consuming plasmid preparation. By using the large-scale screen method in Kit I, all you need ... spending two days using time and labor-intensive analysis methods such as plasmid DNA miniprep or colony hybridization. ... ...
Company:BioChain

loxP flanked Ampicillin Selection Cassette (loxP-amp-loxP) from Gene Bridges GmbH

Description:... by Red/ET Recombination. The loxP-amp-loxP cassette is not linear but plasmid based (3502 bp in size). Due to its R6K origin the plasmid cannot replicate in most E.coli strains. The PCR product can therefore ...
Company:Gene Bridges GmbH

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Plasmid in Biological Definition

Bacterial conjugation

... one of the bacteria has to carry an F- plasmid , the other one must not. The F-plasmid (also called F-factor) is an episome (a plasmid that can integrate itself into the bacterial ... bacterium (5'-end first). The now single-stranded plasmid is filled up with a complementary strand by a ...

Molecular biology

... PCR and/or restriction enzymes ) into a plasmid (known as an expression vector). This plasmid may have special promoter elements to drive ... markers to help follow the plasmid. This plasmid can be inserted into either bacterial or animal ...

Polymerase chain reaction

... which 'carries' the gene into the GEO) such as a plasmid (a circular DNA molecule) (Fig. 5). The DNA can ... A. (4) Insertion of the gene into a plasmid. (5) plasmid with gene from organism A. (6) Insertion of the plasmid in organism B. (7) Multiplication or expression ...

Plasmid

... large plasmids, to hundreds of copies of the same plasmid present in a single cell . Contents ... resistance 2 Episomes 3 Types of plasmid 4 Applications of plasmids 5 See also Antibiotic resistance plasmid often contain genes or gene-cassettes that ...

Antibiotic resistance

... resistance is the ability of a microorganism to withstand the effects of an antibiotic . Antibiotic resistance develops through mutation or plasmid exchange between bacteria of the same species . If a bacterium carries several resistance genes, it is called multiresistant or, informally, a ...

Cytoskeleton

... plasmids encode a partitioning system that involves an actin-like protein ParM . Filaments of ParM exhibit dynamic instability , and may partition plasmid DNA into the dividing daughter cells by a mechanism analogous to that used by microtubules during eukaryotic mitosis . Crescentin The ...

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Plasmid in Biological Dictionary

Plasmid

... A plasmid is an extra-chromosomal DNA molecule separate ...cur... The sequence starting with which the plasmid -DNA will be replicated in the recipient cell. ... F factor as a plasmid independent of the bacterial genome. ... ...

Episome

... encyclopedia (Redirected from Episome ) ... A plasmid is an extra-chromosomal DNA molecule separate ... in the cytoplasm or as part of a plasmid n. A circular, double-stranded unit of DNA that ... is an episome and can exist as an independent plasmid or integrate ... Britannica online ...

DNA clone

... the processes ... DNA Cloning with plasmid Vectors The ... Human Molecular Genetics 2 ... fig. ... Insertion of gene of interest into plasmid to form recombinant DNA ... Brief and ... Abstract for Grants ... DNA cloning projects. plasmid purification. Ordering and ... DNA Cloning ...

Direct Repeats

... orientation ... ... coli recA strains with a plasmid that contains direct repeats of these ... of these direct repeats was inserted into plasmid pACYC184, ... Overlapping direct repeats ... deletions in spedally designed derivatives of plasmid pBR325 ... 17-18 bp and perfect 11 bp direct ...

Cosmid

... Collins and Hohn in 1978, is a type of hybrid plasmid that contains cos sequences, DNA sequences ... A hybrid vector that has been spliced with plasmid DNA for cloning large ... · plasmid · lambda phage · cosmid · P1 phage · ...

Copy number control

... as a mode of negative control of P1 plasmid copy number ... control provides a ... HP Motor ... copy number The number of plasmid or other DNA molecules in a cell. ... What is ... see an increase in the copy number of the plasmid when grown at ... 010: Library of Congress ...

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Other Contents
(Date:10/25/2014)... New York, New York (PRWEB) October 25, 2014 ... of transvaginal mesh lawsuits ( http://www.vaginalmeshlawsuit2015.com/ ) ... multidistrict litigation underway in U.S. District Court, Southern ... Motion for Summary Judgment on Punitive Damages in ... Florida plaintiffs. In a ruling issued on October ...
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(Date:10/25/2014)... Steven Reinberg HealthDay ... The virus that causes common cold sores -- herpes simplex ... by Swedish researchers suggest. In fact, being a carrier ... of Alzheimer,s disease, the researchers found. "The identification ... dementia disorder is a breakthrough," said lead researcher Dr. Hugo ...
(Date:10/25/2014)... FRIDAY, Oct. 24, 2014 (HealthDay News) -- Researchers ... say their findings are cause for concern. ... that are raised for human consumption contribute to ... health, according to background information from the study. ... germs sicken about 2 million people and kill ...
(Date:10/25/2014)... News) -- Current osteoporosis screening guidelines and tools ... risk for osteoporosis-related fractures, a new study says. ... need tools that help us accurately predict who ... can target these at-risk people for preventive measures," ... in the division of general internal medicine and ...
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(Date:10/15/2014)... a non-traditional biophysics textbook and it describes how life ... of discovery into biological systems and provides an understanding ... is about how our genes make proteins that control ... cells in an organism. It quantifies the principles of ... be found on both large and small scales. The ...
(Date:10/15/2014)... , Oct. 15, 2014 NXT-ID, Inc. (NASDAQ: ... authentication company focused on the growing mobile commerce market releases photos ... in Times Square on Monday October 13 th . ... David Tunnel and angel investor Mr. Chad A. ... , CEO of NXT-ID thanked his investors and employees "for their ...
(Date:10/14/2014)... new study published in Cancer Research shows ... and colon cancers—can promote the development of skin cancers ... and survival of sun-damaged skin cells. , Previously considered ... proteins called sirtuins that help regulate genomic stability and ... SIRT6 helps repair DNA damage, which can lead to ...
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