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Phosphodiester in Biological News

Nobel Laureate finds 'elegant' explanation for DNA transcribing enzyme's high fidelity

... on the ß phosphate, the chemical constituent that must have its bond broken in order for the NTP to join the RNA chain through the formation of a phosphodiester bond, Kornberg said. The finding suggested the side chain acts as a trigger for bond formation. The whole decision-making process occurs ...
Phosphodiester in Biological Technology

Decontamination and inhibition of ribonucleases

... as EDTA), ribonucleases (RNases) have virtually no cofactor requirements and advantage of the 2 hydroxyl groups adjacent to the phosphodiester linkages in RNA as a reactive species. Sources for RNase contamination in the Laboratory It is possible to contaminate samples ...
Phosphodiester in Biological Products

T4 DNA Ligase from Invitrogen

Description:... T4 DNA Ligase catalyzes the formation of phosphodiester bonds in the presence of ATP between double-stranded DNAs with 3' hydroxyl and 5' phosphate termini. The unique T4 DNA Ligase buffer optimizes ...
Company:Invitrogen

Calf Topoisomerase I from USB Corp.

Description:... Source: Calf thymus Description: Topoisomerase I relaxes both positively and negatively supercoiled DNA by a transient breakage and rejoining of phosphodiester bonds (1,2) . Relaxing supercoiled DNA with Topoisomerase I allows molecules to be differentiated by a single nucleotide pair by gel electrophoresis ...
Company:USB Corp.

Ligase, T4 DNA from USB Corp.

Description:... Source: E. coli strain containing an overproducing clone of T4 DNA Ligase. Description: T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphoryl and 3'-hydroxyl termini in duplex DNA or RNA. It repairs single-stranded nicks in duplex DNA, RNA or DNA/RNA ...
Company:USB Corp.

Tfi DNA Ligase, 2,000U, thermostable from Bioneer, Inc.

Description:... Tfi DNA Ligase catalyzes the formation of a phosphodiester bonds between adjacent 3-hydroxyl and 5-phosphate termini in nicked duplex DNA molecules or between oligonucleotides which are in duplex with a ...
Company:Bioneer, Inc.

DNase I (RNase-free) (2 U/l) from Ambion

Description:... I (RNase-free) (E.C. 3.1.21.1) is a nonspecific endonuclease that degrades double-and single-stranded DNA and chromatin. It functions by hydrolyzing phosphodiester linkages, producing mono and oligonucleotides with a 5'-phosphate and a 3'-hydroxyl group. Ambion's RNase-free DNase I is of the highest purity ...
Company:Ambion

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Phosphodiester in Biological Definition

Double helix

... DNA is made up of two complementary, antiparallel strands of the bases Guanine , Adenine , Thymine , and Cytosine , covalently linked through phosphodiester bonds . Each strand forms a helix, and the two helices are held together through hydrogen bonds , ionic forces, hydrophobic interactions, and van ...

Okazaki fragment

... DNA replication ). They are later removed by RNAse H , and the last ribonucleotide is removed by and synthesized by Pol I . The nick, or a broken phosphodiester bond remaining between the fragments is linked together by DNA ligase . ...
Phosphodiester in Biological Dictionary

Phosphodiester Bond

... A phosphodiester bond is a group of strong covalent bonds between ... and two other molecules over two ester bonds. phosphodiester bonds are central to all life on Earth, as they ... backbone of the strands of DNA. In DNA ... phosphodiester bond This article does not cite any references ...

Ligase

... DNA Ligase catalyzes the formation of a phosphodiester bond between ... Is Taq DNA Ligase used for a ... technique? ... Catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate ... What ... The T4 DNA Ligase catalyzes the formation of a phosphodiester bond between ... The T4 DNA Ligase binding to ...

DNA ligase

... ... Catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' ... end and ... Catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and ... T4 DNA Ligase catalyzes the formation of a phosphodiester ... T4 DNA Ligase is strongly inhibited by ...

Deoxyribonuclease

... is any enzyme that catalyzes the hydrolytic cleavage of phosphodiester linkages in the DNA backbone. Deoxyribonucleases are thus one type of ... gene DNASE1. DNase I is a nuclease that cleaves DNA preferentially at phosphodiester linkages adjacent to a pyrimidine nucleotide, yielding 5'-phosphate ...

S1 nuclease

... it can also occasionally introduce single-stranded breaks in ... A nuclease is an enzyme capable of cleaving the phosphodiester bonds between the nucleotide subunits of nucleic acids. Older publications may use terms such as "polynucleotidase" or ...

Ribozyme

... A ribozyme is an RNA molecule that catalyzes a chemical reaction. Many natural ribozymes catalyze either the hydrolysis of one of their own phosphodiester bonds, or the hydrolysis of bonds in other RNAs, but they have ... 6 The L1 Ligase Ribozyme Crystal Structure. 6.1 A Three-Dimensional ...

Ribonuclease

... code 2BNH) ... authors methodology in ribonuclease extraction? What is a ... Pancreatic ribonuclease (RNase I) catalyzes cleavage of the phosphodiester bond ... Ribonuclease A has a molecular weight of 13,700 daltons. ... Ahmad, F.: Free Energy Changes in Denaturation of Ribonuclease A by ...

Restriction

... on a ... Restriction enzymes are enzymes that cut DNA at specific recognition sequences ... Restriction enzymes hydrolyze the phosphodiester backbone once on each strand ... Taq I, by the way, is a restriction enzyme from the same type of organism that ... ...

Nuclease

... A nuclease is an enzyme capable of cleaving the phosphodiester bonds between the nucleotide subunits of nucleic acids. Older publications may use terms such as "polynucleotidase" or ...

Exonuclease

... are enzymes that work by cleaving nucleotides one at a time from the end of a polynucleotide chain. A hydrolyzing reaction occurs that breaks phosphodiester bonds at either the 3’ or 5’ ends. Its close ... exonuclease activity. • protein binding. • hydrolase activity ... Human ...

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