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Phagemid in Biological Technology

Using living cells as nanotechnology factories

... particle that infects a bacteria cell. Once inside the cell, the phagemid used the cell just like a photocopier machine to reproduce millions of copies of the DNA. By theoretically starting with just a single phagemid infection, and a single milliliter of cultured cells, Yan found that the ...

Pharmexa and Affitech to Combine Operations to Create a New Antibody Therapeutics Company

... "Affitech has developed a fully integrated antibody discovery platform consisting of human antibody libraries with high functionality, based on phagemid display technology plus several screening technologies with high degree of versatility. Through partnerships with both pharma and biotech businesses, ...

A New Lambda Vector for Mammalian Expression

... Lambda ZAP II vector and the pCMV-Script -EX phagemid vector. High-efficiency cDNA libraries ... expression in the pCMV-Script-EX phagemid vector. The Lambda ZAP-CMV vector ( ... cloning site, and the pCMV-Script-EX phagemid vector. Figure ...

M13/Phagemid DNA Extraction Protocol

... agitation for good aeration of the culture. For producing phagemid ssDNA, suspend a colony of phagemid transformed bacteria in a 15 ml culture tube containing 3 ...

M13/Phagemid DNA Extraction Protocol

... agitation for good aeration of the culture. For producing phagemid ssDNA, suspend a colony of phagemid transformed bacteria in a 15 ml culture tube containing 3 ...

TripleMaster PCR System

... high fidelity amplifications of smaller targets ranging from 100 bp to 10 kb with genomic templates and up to 15 kb with plasmid or phagemid DNA. Control reagents are supplied with the TripleMaster PCR System Plus for the amplification of a 40 kb fragment. ...

TripleMaster PCR System

... high fidelity amplifications of smaller targets ranging from 100 bp to 10 kb with genomic templates and up to 15 kb with plasmid or phagemid DNA. Control reagents are supplied with the TripleMaster PCR System Plus for the amplification of a 40 kb fragment. ...

Restriction Endonucleases Undergo Rigorous QC Testing to Ensure Optimal Performance

... models the use of restriction enzymes is the blue-white color selection assay. In this assay, Stratagenes pBluescript II SK phagemid vector ( Figure 3 ), which contains the lacZ reporter gene, is cut at a single position within the multiple cloning site using ...

Purified Single-Stranded M13 DNA for Automated Sequencing

... realize high-quality automated fluorescent sequencing results as well. Additionally, the kit can be used to purify single-stranded DNA from rescued phagemid clones. REFERENCES Richards, S., et al. (1994) In Automated DNA Sequencing and Analysis (Eds. Adams, M.D., et al.) pp. 191-198. ...

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Phagemid in Biological Products

Kilo Sequencing Deletion Kit from Takara Mirus Bio

Description:... to aid sequence analysis of long DNA fragments cloned into the multiple cloning sites (MCS) of M13 phage vectors (mp18/19) or pUC-related plasmid or phagemid vectors (pUC18/19). The kit is used to create nested unidirectional deletions in the target DNA, thereby progressively moving the primer binding site ...
Company:Takara Mirus Bio
Phagemid in Biological Definition

Phage

... are extensively studied: λ phage - Lysogen T4 phage (169 to 170 kbp , 200 nm long) T7 phage R17 phage M13 phage - phagemid See also Horizontal gene transfer One-step growth curve ...
Phagemid in Biological Dictionary

Phagemid

... A phagemid or phasmid is a type of cloning vector ... as an f1 ori to enable single ... phagemid A phagemid or phasmid is a type of cloning vector ...

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(Date:4/17/2014)... signs of chronic inflammation in non-cancerous prostate tissue may ... cancer than those with no inflammation, according to results ... Johns Hopkins Kimmel Cancer Center. , The link between ... with so-called high-grade prostate cancer those with a ... presence of the most aggressive and rapidly growing prostate ...
(Date:4/17/2014)... study published today in PLOS Pathogens , children ... is common can mount an immune response to infection ... repeated bouts of high fever and illness and partially ... The findings may help researchers develop future interventions that ... parasite. , Each year, approximately 200 million cases of ...
(Date:4/17/2014)... the April issue of Immunity , Kevin J. ... Research, says it,s time to take a fresh look ... sepsis, which kills millions worldwide every year, including more ... into the bloodstream to fight an injury or infection ... maintaining good health without inflammation, wounds and infections ...
(Date:4/17/2014)... (April 17, 2014) Doctors who treat patients ... lymphangioleiomyomatosis (LAM) can face an agonizing treatment decision. ... the disease and help relieve shortness of breath. ... and sirolimus can cause potentially fatal complications following ... Dr. Daniel Dilling, medical director of Loyola University ...
(Date:4/17/2014)... By discovering a new mechanism that allows blood to ... UC Irvine and the Salk Institute have opened the ... stroke-induced brain damage. , A complex and devastating neurological ... primary reason for disability in the U.S. The blood-brain ... blood-borne material into the brain, causing the permanent deficits ...
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(Date:4/20/2014)... Mothers give a newborn baby a gift of ... system. But antibiotics, used to fend off infection, ... leaving already-vulnerable premature babies more susceptible to dangerous ... neonatology researchers at The Children,s Hospital of Philadelphia ... revealing how gut microbes play a crucial role ...
(Date:4/18/2014)... decades, scientists have used techniques like X-ray crystallography and ... into the atomic structure of molecules, but such efforts ... demand large quantities of a specific molecule and often ... making it all but impossible to peer into the ... those problems may soon be a thing of the ...
(Date:4/18/2014)... Durham, NC Seeds that sprout as soon as they,re ... plants need to be more careful. In the wild, a ... rainy day would risk disaster. More than just an insurance ... out that seed dormancy has long-term advantages too: Plants ... give rise to more species, finds in a team of ...
Breaking Biology News(10 mins):Study of gut microbes, antibiotics: Clues to improving immunity in premature infants 2MRI, on a molecular scale 2MRI, on a molecular scale 3Plants with dormant seeds give rise to more species 2
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