Cytoplasm affects the number of vertebrae in carp-goldfish clones
...triguing findings in cloned offspring created when nuclei from one genus of fish were transplanted to enucle...r genus of fish. The seven offspring, cloned from nuclei of common carp and egg cytoplasm of goldfish, were virtually identical to the nuclear donor species,...Color-blind method opens new doors in DNA sequencing
... sequencing, scientists first extract DNA from the nuclei of cells and through a painstaking series of bacterial cloning and/or polymerase chain reaction (PCR) steps, reduce its length to a manageable size of thousands of nucleotides. Using natural replicating enzymes, the DNA is tagged with four fluorescen...Nano-Probes Allow an Inside Look at Cell Nuclei
...loped a way to sneak nano-sized probes inside cell nuclei where they can track life's fundamental processes,...biologist can image long-term phenomena within the nuclei of living cells," says Fanqing Chen of Berkeley Lab's Life Sciences Division, who developed the tech...Research Using Mouse Models Reveals A Novel Key Player In The Initiation Of Colon Cancer
...cumulation of a protein called beta-catenin in the nuclei of these cells. Kaestner's group had earlier published research on a transcription factor called Foxl1 that is also expressed in the colon, but in a different layer of cells, adjacent to the epithelium, called the mesenchyme. They had seen that mice...Cooperation is key—a new way of looking at MicroRNA and how it controls gene expression
...e tens of thousands of stretches of DNA within the nuclei of cells we call our genes. One thing that has become clear in the last several decades, however, is that while our genes contribute to human health and disease, it is not always the genes themselves that matter, but rather how the genes are control...Qproteome Nuclear Subfractionation Kit from QIAGEN
...leic-acid binding proteins Efficient separation of nuclei from cytosolic proteins A huge reduction in sample complexity, greatly facilitating study of low-abundance nuclear proteins Subfractionation of nucleic acid binding proteins (e.g., transcription factors and other proteins involved in gene regulation)...Artisan™ Masson's Trichrome Stain Kit from Dako
...lagen - blue, fibrin - pink, erythrocyte - red and nuclei - blue/black. Applications: Histological stains Additional Information: EU regulatory status: CE US regulatory status: IVD...Mouse Anti-CDX-2 Monoclonal Antibody, Unconjugated, Clone CDX2-88 from BioGenex
...ein localized in the nucleus. It exclusively marks nuclei of colonic epithelial cells and colorectal cancers on formalin-fixed, paraffin-embedded tissue sections. Therefore clone CDX2-88 may be used in identifying metastatic colon carcinoma....New Protocols for Isolating High- Molecular-Weight Genomic DNA
...ular debris(<3 minutes), isolation of the cell nuclei by centrifugation (<20minutes), and protease digestion of the nuclei (45 minutes). 1,2 Theresulting suspension is transferred to a free-floating RecoverEase kit cup whe...Principles and applications of electrical cell fusion
...he outer cell membrane has fused, two or more cell nuclei are still present. The cell nuclei also fuse at a later point within the cell. In most cases, this results in a drastic reducti...Genomic DNA Isolation Protocol(1,2,3)
...ons containing genomic DNA. I Isolation of nuclei Nuclei from blood Caution: Wear...on for 5 minutes on ice. Pellet nuclei by centrifugation at 1000 x g for 12 minutes at 4C. Decant supernatant ......he outer cell membrane has fused, two or more cell nuclei are still present. The cell nuclei also fuse at a later point within the cell. In most cases, this results in a drastic reducti...Compressed DNA aids gene patients
...re effective at getting their genes working in the nuclei of cells than DNA-carrying liposomes. Now basic safety trials have begun involving human patients, suffering from cystic fibrosis also. This disorder, which involves faulty production of the mucus which lines the lungs, is caused by a single fault...Enzyme help destroy cancer cell
...While it was thought that damage to the DNA in the nuclei of tumor cells// was the only way that radiation killed tumor cells, this research shows another radiation-induced pathway initiated when radiation strikes endothelial cells....Detecting DNA Damage With In-Vivo Alkaline Comet Assay
... main requirement of the assay is a single cell or nuclei suspension, which can be obtained from the tissue without adding to the DNA damage by the applied isolation technique. However, the assay only measures DNA damage and it remains unclear if mutations will result or whether the detected damage will be ...Research identifies trigger that sets of Huntingtons disease
...he research findings thus link the disturbances of nuclei and mitochondria in the disease condition. Reference: Neuron, July 2005...... Dolly. To clone Snuppy, the researchers implanted nuclei from his father’s ear cells into eggs from female dogs, having removed the eggs’ nuclei. By passing a small electric shock to start development, the embryos were implanted into the uterus of a surrogate mother, which was a labrador dog. The team used...