Scientists ID molecular 'switch' in liver that triggers harmful effects of saturated and trans fats
...at was isolated in 2002. Experiments including the measurement of gene activity by microarrays showed that saturated and trans fats caused greater activity of the gene that makes PGC-1beta co-activator than did unsaturated fats. The research also showed that when the fats triggered PGC-1beta, the co-activator i...Recent breakthroughs in common adult leukemia highlighted in New England Journal of Medicine
...for clinical use, however according to the review, measurement of one protein marker called ZAP-70 is becoming more widely available. The authors note that ZAP-70 may actually be the most reliable indicator of prognosis known to date. After discussing B lymphocytes -- the type of white blood cell that gives ris...Devising Nano Vision for an Optical Microscope
...0-year-long record as an indispensable imaging and measurement tool well into the expanding realm of nanotechnology. Called phase-sensitive, scatter-field optical imaging, the computer-intensive technique under development at NIST uses a set of dynamically engineered light waves optimized for particular propert...Affymetrix and the Karolinska Institutet Announce Translational Medicine Strategic Alliance
...ve years the projects include genetic analyses and measurement of gene expression in patients with atherosclerosis, breast cancer, rheumatoid arthritis, asthma and dyslexia. These diseases affect a large number of patients worldwide. Systematic use of the genomic information obtained will enable development of n......ardiologists a new view of subtle changes in a key measurement of heart health -- heart rate variability. Patients with little variability -- whose hearts are unable to appropriately react to external stimuli by regulating their beating action -- are known to be at higher risk of suffering a heart attack. The ne...Glutathione-S-Transferase (GST) Colorimetric Assay Kit from Sigma-Aldrich
...: The GST Assay is intended for total GST activity measurement in cell and bacterial lysates, tissue homogenates, and in plasma and erythrocytes lysates. Kit utilizes 1-Chloro-2,4-Dinitrobenzene (CDNB) which is suitable for the broadest range of GST isozymes. Upon conjugation of the thiol group of glutathione to...Standard Protein Kinase Substrate (PKS) Peptide Microarray from LC Sciences
...for proteomic scale kinase profiling, quantitative measurement of kinase kinetic activities, and drug discovery research built on the flexible and powerful Paraflo microfluidic on-chip synthesis platform. These microarrays are available as part of our comprehensive Protein Kinase Substrate - Peptide Microarray S...Protein Kinase Substrate (PKS) Peptide Microarray - Comprehensive Service from LC Sciences
...for proteomic scale kinase profiling, quantitative measurement of kinase kinetic activities, and drug discovery research. This comprehensive service includes preparation of your kinase sample, single or dual color enzymatic reactions, array scanning and data analysis. Two-three weeks after receiving your kinase ...Agilent 7500ce from Agilent Technologies
... in estuarine and open ocean seawater ultratrace measurement of Se and Se isotope ratio analysis in biological sample matrices ultratrace measurement of As, Se and Cr in clinical sample matrices The 7500ce incorporates a new digital-drive RF generat......tomated patch clamp systems. The 48-channel QPlate measurement plate builds on the same technology as the original 16-channel QPlate. The individual measurement sites include glass-coated microfluidic channels for less compound absorption and hence more accurat...Enzyme Immunoassay for Studying Intracellular Levels of cAMP
...y-based assay kit for nonradioactive, quantitative measurement ofintracellular levels of cAMP. The assay is based... figure 1 ) assay for simple and sensitive measurement of intracellualar cAMP levels. The assay is based on the competition between cAMP present in...Imaging Chemiluminescent Western Blots
... ULTRA substrate had an integrated density measurement equivalent to a 216-ng band using ECL substrate, based on the equation of a standard curve generated when all four of the visible ECL bands were used as standards. This equates to an 11.5-fold increase in sensitivity observed ...A Comparison Study of Lipid Tranfection Reagents in A549, NIH 3T3 and COS-7 Cell Lines
... transfection reagent as determined by the measurement of the total protein for the various volumes used. Even for 20 l of LipoTAXI transfection reagent, the concentration of cell protein was constant. For the other transfection agents, toxicity to cells increased as their amounts...Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
...ction conditions, preparation of cell lysates, and measurement of b -galactosidase activity were performed as previously described. 3 In these experiments, increased b -galactosidase activity resulted from using higher DNA concentrations. However, as the concentration of DNA in......e BioPhotometer. Absorption measurement at 280 nm (A 280 ) A 280 method may be used with in concentrations of up to approximately 4 mg/ml (3.0 A). This method is simple and rapid, but may be disturbed by the parallel absorption of non-proteins (e.g. DNA). Unlike the col...Low Cholesterol Linked to Severe Depression and Violent Behavior in Men
... Durham, N.C. Said, "I think that at some point, measurement of cholesterol and the cholesterol profile may be used as a screening tool for depression," Vivian Mitropoulou, PhD, who is studying the link between cholesterol levels and personality disorders at Mount Sinai School of Medicine in New York told that...Watch your weight when pregnant
...d calculated each woman's body mass index (BMI), a measurement of body fat calculated using a formula that is based on height and weight, and related it to her chances of having a Cesarean section. Women requiring the surgery were an average weight of 191 pounds and had an average BMI of 53, while women who del...Avoid stress to have strong babies
...normal blood flow patterns. A "resistance index" measurement was taken of the extent to which the blood flow was impaired.The researchers found that of the most anxious group, 28% had a resistance index high enough to be of "clinical concern". Only 5% in the less anxious group had similarly impaired uterine ar......s the top number in a blood pressure reading. This measurement represents the highest level of the blood's pressure within the artery walls and corresponds to the contraction of the heart's main pumping chambers (ventricles). Higher insulin resistance was related to a higher systolic blood pressure in the the15-......s the top number in a blood pressure reading. This measurement represents the highest level of the blood's pressure within the artery walls and corresponds to the contraction of the heart's main pumping chambers (ventricles). Higher insulin resistance was related to a higher systolic blood pressure in the the15-...PSA TestingHow Much is Too Much?
...0 years or older. The assay provides equimolar PSA measurement based on a monoclonal antibody pair. The company lists analytical sensitivity at <0.008 ng/mL ; measurement range to 150 ng/mL and precision at < 5% CV across the assay range. Time to first result is 20 ...Hemoglobin Testing at the Point of Care
...can be an essential element of patient assessment, measurement of hemoglobin levels in the clinical setting is of...d prothrombin time, prompt, easily used hemoglobin measurement systems now provide a virtual hematology laboratory at the point of care....Point of Care Cardiac Analyzers Yield Rapid Cardiac Marker Results
...ng or ruling out acute myocardial infarction. The measurement of cardiac troponins is especially useful in the l...The indications for use are for rapid quantitative measurement of markers to aid in the diagnosis of myocardial injuries. Biosite Inc. offers the Triage Cardiac Pa...Tight Glycemic Control in the ICU
...handheld devices that provide quantitative glucose measurement of capillary, venous, arterial and neonatal whole ...ation. Accurate results are claimed for the whole measurement range of 0-444 mg/dl. Quality control (QC) must be performed with the HemoCue control cuvette and c...Near-Patient Testing: The Advantages of C-Reactive Protein in Hematological Testing
...cognized as a marker of systemic inflammation. CRP measurement can complement a range of diagnostic markers to mo... recovery time. Complete Blood Count (CBC) and CRP measurement are two possible hematological tests that could be relocated from the clinical laboratory to the pat...