Engineering chimeric polypeptides to illuminate cellular redox states

... major obstacle to a FRET-based redox sensor. The researchers designed the linker sequence such that in its reduced state the linker is an α-helix. Thiol groups, strategically placed throughout the ...

UGA researchers 1 step closer to elusive cancer vaccine

... The immune system, perhaps not surprisingly, attacked the protein and the linker molecules, but generally left the carbohydrate alone. We needed to come ... cells, a part of a protein that triggers the immune systems T cells and a linker molecule that stimulates the production of generalized immune components ...

Mersana's Second Development Candidate, XMT-1107, Shows Superior Pharmacokinetics and Anti-Tumor Activity in Studies Presented at American Association of Cancer Research Meeting

... the clinic but were discontinued due to reversible neurological toxicity. Conjugation of our novel fumagillin 'warhead' to Fleximer using a specific linker technology reduces CNS exposure in animal models to below detection limits. We look forward to advancing XMT-1107 into clinical studies by early ...

Motor proteins may be vehicles for drug delivery

... motors than in other kinesin proteins, which prevents efficient mechanical coordination between the two motor domains. "If you think of this linker domain as a taut bungee cord, any force at one end will be communicated very efficiently to the other end. So the two motors can communicate very ...

American Chemical Society's Weekly PressPac -- Feb. 6, 2008

... tests, they say. MTS ARTICLE #1 FOR IMMEDIATE RELEASE Protein Recording Material: Photorecord/Erasable Protein Array Using a UV-Eliminative linker DOWNLOAD FULL TEXT ARTICLE http://dx.doi.org/10.1021/la703354c CONTACT Tetsuro Majima, Ph.D. The Institute of Scientific and Industrial ...

Taxol bristle ball: a wrench in the works for cancer

... they needed to perform to create the ball. Using the wrapped version of the drug, they undertook a series of reactions to attach the drug to linker molecules that were, in turn, attached to the ball. In the final step of the reaction, they dissolved the wrapper, restoring the key. "We are ...

Study Findings Could Help In Developing A Better Drug Delivery System

... the folate-linked complex enters the cell," Yang said. "This 'conditional drug release' is usually realized by attaching folate to the drug through a linker that falls apart inside the cell. There were several linkers in common use, but with mixed efficiency. In this study we undertook to interrogate the ...

Novel Technique For Targeting Small-Cell Lung Cancer

... based on backbone cyclic peptides, and conjugated them with fluorescent agents. Nineteen conjugates differing in core peptide, length of alkyl linker and fluorescence moiety (rhodamine and fluorescein) were tested in vitro, using a receptor binding assay, and nine of the more promising conjugates ...

Linker in Medical Technology

Medarex Announces Preclinical Data from Multiple Research Programs Demonstrating Anti-Tumor Activity in Cancer

... #1722) The effectiveness of a cleavable linker over a non-cleavable linker when used with MGBA-based antibody-drug ... treatment with conjugates using the cleavable linker demonstrated selective and antigen-dependent ...

CuraGen Announces Expansion of CR011-vcMMAE Phase II Trial in Advanced Breast Cancer

... monoclonal antibody, CR011, linked to a potent cell-killing drug, monomethyl-auristatin E (MMAE). The ADC technology, comprised of MMAE and a stable linker system for attaching it to CR011, was licensed from Seattle Genetics, Inc. The ADC is designed to be stable in the bloodstream. Following ...

CuraGen Updates CR011-vcMMAE Data at ASCO

... monoclonal antibody, CR011, linked to a potent cell-killing drug, monomethyl-auristatin E (MMAE). The ADC technology, comprised of MMAE and a stable linker system for attaching it to CR011, was licensed from Seattle Genetics, Inc. The ADC is designed to be stable in the bloodstream. Following ...

CuraGen to Present CR011-vcMMAE Data at ASCO

... monoclonal antibody, CR011, linked to a potent cell-killing drug, monomethyl-auristatin E (MMAE). The ADC technology, comprised of MMAE and a stable linker system for attaching it to CR011, was licensed from Seattle Genetics, Inc. The ADC is designed to be stable in the bloodstream. Following ...

CuraGen Announces Preliminary Data from Ongoing Phase II Study Showing Clinical Activity of CR011-vcMMAE in Patients with Metastatic Melanoma

... monoclonal antibody, CR011, linked to a potent cell-killing drug, monomethyl-auristatin E (MMAE). The ADC technology, comprised of MMAE and a stable linker system for attaching it to CR011, was licensed from Seattle Genetics, Inc. (Nasdaq: SGEN ). The ADC is designed to be stable in the bloodstream. ...

Medarex Highlights Strength in Antibody Development and Underlying Pipeline Strategy at R&D Day Event in New York City

... (ADC) platform. Medarex believes that its ADC technology overcomes many of the key development challenges of drug conjugates, including issues of linker stability, potency and multi-drug resistance while maintaining a wide therapeutic window with minimal toxicity. Coupled with cutting-edge antibody ...

CuraGen Announces Expansion of CR011-vcMMAE Phase II Trial in Melanoma

... is a novel, selective target that plays a role in the ability of cancer cells to invade and metastasize. CR011-vcMMAE contains an enzyme-cleavable linker designed to be stable in the bloodstream and to release MMAE after internalization into tumor cells that express GPNMB, resulting in cancer cell ...

CuraGen Announces Data From Ongoing Phase I/II Trial Showing Clinical Activity of CR011-vcMMAE in Metastatic Melanoma

... monoclonal antibody, CR011, linked to a potent cell-killing drug, monomethyl-auristatin E (MMAE). The ADC technology, comprised of MMAE and a stable linker system for attaching it to CR011, was licensed from Seattle Genetics, Inc. (Nasdaq: SGEN ). The ADC is designed to be stable in the bloodstream. ...

CuraGen Advances CR011-vcMMAE into Phase II

... a novel, selective target that plays a role in the ability of cancer cells to invade and metastasize. CR011-vcMMAE contains an enzyme-cleavable linker designed to be stable in the bloodstream and to release MMAE after internalization into tumor cells that express GPNMB, resulting in cancer cell ...

CuraGen Announces Presentation of Phase I Dose-Escalation Results on CR011- vcMMAE for Metastatic Melanoma

... monoclonal antibody, CR011, linked to a potent cell-killing drug, monomethyl-auristatin E (MMAE). The ADC technology, comprised of MMAE and a stable linker system for attaching it to CR011, was licensed from Seattle Genetics, Inc. (Nasdaq: SGEN ). The ADC is designed to be stable in the bloodstream. ...

Linker in Biological News

UC San Diego engineer provides insights to decades-old DNA squabble

... along the chain lie side by side on the chromatin fiber also and thus the linker DNA connecting the two needs to maintain a highly bent conformation. Other ... on the chain actually lie on opposite sides of the fiber such that the linker DNA is straight. (The nucleosome is the basic unit of chromatin that ...

Flexible neck in cell-receptor DC-SIGN targets more pathogens

... to adapt to the target surface and maximize the bond. This 5 nanometer conformational change is binding-induced, and made possible by a flexible linker in the neck, the researchers report. "The protein neck region acts as a stiff, but flexible, rod that projects the molecule's binding sites away ...

'Disordered' amino acids may really be there to provide wiggle room for signaling protein

... work. That is, it allows the PDZ binding module to "bite" its own ezrin-binding tail, effectively shutting the protein down. "The flexibility of the linker and its tendency to be more or less disordered are critical for regulating the balance between internal ("autoinhibitory") forces and external ...

Nanoneedle is small in size, but huge in applications

... gold. Molecular cargo is then attached to the gold surface via "linker" molecules. When placed in a cell's cytoplasm or nucleus, the bonds with the linker molecules break, freeing the cargo. With a diameter of approximately 50 nanometers, the nanoneedle introduces minimal intrusiveness in penetrating ...

NYU chemists use computer simulation to enhance understanding of DNA transcription

... and her colleagues, these results revealed that in a low-salt environment, linker DNAs in the array were repelled, preventing array folding and resulting in a bead-like structure. However, under high-salt conditions, screening of linker DNA repulsion allows close contacts and attraction between nucleosomes, ...

Scientists identify molecular cause for one form of deafness

... Filamentous actin (F-actin) is a rod-like protein that provides structural framework in living cells. F-actin is organized into bundles by espin, a linker protein found in sensory cells, including cochlear hair cells. Genetic mutations in espin's F-actin binding sites are linked to deafness in mice and ...

Nanoparticle assembly enters the fast lane

... can greatly slow the assembly process. In the Brookhaven study, researchers introduced partially rigid, double-stranded DNA, which forces interacting linker segments of DNA to extend away from the gold surface, allowing for more efficient assembly. "By using properties of DNA, we can increase ...

ASU researchers 'wire' DNA to identify mutations

... simple setup. You just bring two electrodes together, separate them apart, make the measurement and repeat." In the technique, chemical linker groups that form a tight bond with gold electrodes are attached to the ends of DNA. A drop of a DNA solution is then placed between the two ...

'Smart' nanoprobes light up disease

... steadily returned. Ultimately, the researchers hope to pair a series of quantum dots, each with a unique NIR optical signature, to an index of linker proteases. "There is currently a critical need for methods to simultaneously image the activity of multiple proteases in vivo," said Drezek. "This ...

Quantum dots provide a faster, more sensitive method for detecting respiratory viral infections

... dots are available in a dozen different colors, and antibodies specific to the other four respiratory viruses have been identified and can be used as linker molecules. Such a test would be able to diagnose more than 90 percent of all the cases of viral respiratory infection, he says. The existence of ...

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Linker in Biological Technology

DNA is blueprint, contractor and construction worker for new structures

... single-stranded portion of this DNA serves as the linker DNA, which seeks out a complementary single ... The binding of the two single strands of linker DNA to each other completes the double helix, ... structure -- a crystal. One sequence of linker DNA, programmed by the researchers, results in ...

Medarex Announces Allowance of Investigational New Drug Application for Company's First Antibody-Drug Conjugate, MDX-1203

... linker. Medarex believes that its ADC technology overcomes many of the key development challenges of drug conjugates, including issues of linker stability, potency and multi-drug resistance while maintaining a wide therapeutic window with minimal toxicity. Medarex has conducted multiple ...

Transgenomic Awarded SBIR Grant to Support Development of SURVEYOR(R) Endonuclease Adaptor-ligated Libraries (SEAL) for Determining Whole Genome Sequence Variation

... for whole genome analysis under development by Transgenomic. It identifies DNA variations between a reference genome and a test genome. Using a linker that attaches an identification tag of known sequence to the DNA ends processed by the SURVEYOR Nuclease, SEAL generates a library of genetic ...

Transgenomic Introduces SURVEYOR(R) Endonuclease Adaptor-Ligated Libraries (SEAL) for Determining Whole Genome Sequence Variation

... for whole genome analysis under development by Transgenomic. It identifies DNA variations between a reference genome and a test genome. Using a linker that attaches an identification tag of known sequence to the ends cleaved by the SURVEYOR Nuclease, SEAL generates a library of genetic variations ...

Taxol bristle ball: a wrench in the works for cancer

... they needed to perform to create the ball. Using the wrapped version of the drug, they undertook a series of reactions to attach the drug to linker molecules that were, in turn, attached to the ball. In the final step of the reaction, they dissolved the wrapper, restoring the key. "We are ...

Monitoring fluorescence resonance energy transfer (FRET) between GFP fusions in lysates of the yeast Saccharomyces cerevisiae using the Varian Cary Eclipse

... GFP linked by a 27 amino acid peptide linker that contains a recognition site for ... 25C to promote cleavage of the peptide linker by trypsin (Figure 2). Using the Scan ... addition of trypsin, which cleaves the peptide linker that tethers the GFPs. A small ...

Inactivation of Contaminating Templates in PCR Using High Intensity Ultraviolet Light

... sterilization method using the GS Gene linker UV chamber to destroy contaminating amplicon or ... placed in the middle of the GS Gene linker oven and were either capped and placed on their ... UV light was delivered by the GS Gene linker chamber for 30 minutes. After UV irradiation was ...

UV Nicking of DNA in Agarose Gels for Enhanced Transfer and Detection of Megabase Size DNAs

... to HCl nicking. The GS Gene linker UV chamber contains preprogrammed routines for ... with 60 mJoules of energy in the GS Gene linker UV chamber after staining with ... on a glass tray for transportation to the GS Gene linker chamber, place the tray inside the GS ...

Synthesize High Yields of Biotinylated aRNA

... single biotin UTP label. While various linker arm sizes (e.g., Biotin-11-UTP, Biotin-16-UTP) did ... UTP incorporation into aRNA during IVT, the longer linker arms slightly impeded subsequent purification of the ...

Genomic Analysis of Formalin-Fixed Paraffin Embedded (FFPE) Tissues through the use of Whole Genome Amplification (WGA)

... relatively short lengths of nucleic acid stretches, ~1kb or less. Four WGA methods have been described and are in common use they include linker adapter PCR (LAP) 8-10 , primer extension preamplification PCR (PEP) 11 , degenerate oligonucleotide PCR (DOP) 12,13 and multiple strand ...

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Linker in Biological Products

Weinreb Linker from ChemPep, Inc.

Description: Weinreb ...
Company:ChemPep, Inc.

Linker in Biological Definition

Restriction enzyme

... potential " restriction sites " appear in almost any gene or chromosome . Meanwhile, the sequences of some artificial plasmids include a " linker " that contains dozens of restriction enzyme recognition sequences within a very short segment of DNA. So no matter the context in which a gene ...

Linker in Biological Dictionary

Linker

... In computer science, a linker or link editor is a program that takes one or ... them into a single executable program. The linker resolves all of the unresolved addresses into ... to Linkers by David Drysdale ... What The linker Does: Part 1. Duplicate Symbols. What The ...

Histone

... " Go to Millipore's home page ... The linker histone , H1, interacts with linker DNA between nucleosomes and ... This gene is ... H4 - form ... Histone H1 also associates with linker DNA between nucleosomes to stabilize ... ...

Nucleosome

... |Hardcover|Science|Elsevier Science Ltd|A. Wolffe The Nucleosome Books Chromatin. ... II- The nucleosome ... This core particle and the linker DNA together form the nucleosome . ... The fundamental packing unit is known as a nucleosome . ... code for nucleosome positioning. ...

Dynein

... complex responsible for the transport of a large ... Dynein , along with kinesin (see previous ALS highlight, "Kinesin Action ... AAA+ Ring and linker Swing Mechanism in the Dynein Motor ... Single-Molecule Analysis of Dynein Processivity and Stepping Behavior ... Single-Molecule Analysis of ...

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