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Immunoprecipitation in Medical News

BioInformatics, LLC New Report - Exploring the Epigenetics Market: Opportunities for Product Placement and Innovations

... and Innovations. Designed to provide guidance for life science suppliers selling products that support epigenetic research involving chromosome immunoprecipitation (chIP), this report will help suppliers optimize product offerings and strengthen market position. For this report, BioInformatics, LLC ( ...

Novel DNA vaccine leads to kidney damage prevention in systemic lupus erythematosus models

... Serum samples were collected at day 0 and then monthly for analysis of autoantibodies and immunoglobulin levels. Serum autoantibodies were tested by immunoprecipitation and ELISA. Proteinuria (excessive protein in the urine) was assessed monthly using Multistix. Kidney pathology and immune complex deposition were ...

Farnesoid X receptor regulates cystathionase

... and her colleagues in the University of Perugia, Italy used luciferase transfection assay, Electrophoretic Mobility Shift Assay (EMSA), Chromatin immunoprecipitation Assay and Real-Time PCR to confirm the role of FXR in the regulation of CSE transcription. The molecular mechanism of the CSE activation by FXR ...

Penn researchers find a new role for a 'Foxy Old Gene'

... resides in the Department of Genetics and the Institute for Diabetes, Obesity, and Metabolism at Penn. ChIP-on-Chip assay stands for Chromatin immunoprecipitation on a gene Chip. First, in the two-step assay, chromatin (DNA and protein) was extracted from liver cells and an antibody specific for FOXA2 was ...

Cold Spring Harbor Protocols features high-throughput methods for analyzing gene activity

... looks at the binding of regulatory proteins to DNA. These proteins play a role in turning activity of individual genes on and off. The method, "DNA immunoprecipitation (DIP) for the Determination of DNA-binding Specificity," allows researchers to determine the specific DNA sequence that a regulatory protein binds. ...

New Tool for Understanding Estrogen-Fueled Breast Cancer

... ER. The binding-site map was compiled with a novel two-step procedure called ChIP-on-chip. Researchers first used a technique called chromatin immunoprecipitation (ChIP) to purify the regions of genes that take orders from the ER. They then placed these pieces of DNA on "microarray chips" containing the entire ...
Immunoprecipitation in Biological News

Researchers expand efforts to explore functional landscape of the human genome

... Elements. Utilizing the technique of chromatin immunoprecipitation followed by high-throughput DNA sequencing, this ... isolation of regulatory elements and chromatin immunoprecipitation for a few key DNA-binding factors. Chromatin is ... factor binding sites by using chromatin immunoprecipitation followed by high-throughput sequencing, and to ...

New edition of laboratory manual includes cutting-edge techniques to study gene regulation

... a newly isolated gene and the biochemical characteristics of a new transcription factor. In addition to chromatin-based techniques such as chromatin immunoprecipitation (ChIP), the new edition incorporates RNA interference (RNAi), mass spectrometry, and bioinformatic and experimental methods that exploit genome ...

Tracking the molecular pathway to mixed-lineage leukemia

... activities, Matthew Guenther, a postdoctoral researcher in Whitehead Member Richard Young's lab, used a technique called ChIP-seq (chromatin immunoprecipitation with DNA sequencing) to map where the MLL-AF4 protein interacts with a cell's genome. He found that in cancer cells the MLL-AF4 protein binds to at ...

Scientists shed light on evolution of gene regulation

... among mammals do act to control the level of expression of certain genes. To make their discovery, the team used a technique called chromatin immunoprecipitation (ChIP) to identify all the DNA segments that bind to the GATA1 protein along most of a mouse chromosome. The technique works by using an antibody ...

NIA uses Genomatix in stem cell research, suggests novel transcription factors for stemness

... increased likeliness for functional significance of the candidate transcription factors. Predictions were experimentally verified by Chromosomal immunoprecipitation assays. The study led to the novel identification of two transcription factors, B-Myb and Maz which are implicated either in the maintenance of ...

DNA repair proteins monitored at double-strand break

... they could better control when the enzyme moves into the nucleus and cleaves the DNA. The team then used a biochemical technique called chromatin immunoprecipitation to collect and identify repair proteins and show where each one bound to the DNA. ...

Molecular atlas provides new tool for understanding estrogen-fueled breast cancer

... ER. The binding-site map was compiled with a novel two-step procedure called ChIP-on-chip. Researchers first used a technique called chromatin immunoprecipitation (ChIP) to purify the regions of genes that take orders from the ER. They then placed these pieces of DNA on "microarray chips" containing the entire ...
Immunoprecipitation in Biological Technology

Sigma(R) Launches Innovative ChIP Kit Technology For Epigenetic Research

... Kit. This flexible format allows for immunoprecipitation and purification of DNA from mammalian cells or ... all of the requirements for Chromatin immunoprecipitation procedures," said Tim Fleming, Director of Global ... The Imprint(TM) ChIP Kit is based on Chromatin immunoprecipitation (ChIP) technology licensed from Epigentek Group. ...

Frost & Sullivan Awards Genpathway 2008 Healthcare Innovation Award

... in Drug Discovery Technologies Healthcare Innovation Award. The award recognizes Genpathway for providing superior services for chromatin immunoprecipitation (ChIP) and DNA methylation assays that directly contribute to discoveries in gene regulation, epigenetics, and gene transcription. "Frost & ...

Quantitative Analysis of Protein-DNA Associations in Vivo Using Real-Time PCR, Rev A

... Introduction The chromatin immunoprecipitation (ChIP) assay has been used in recent ... Method Chromatin immunoprecipitation assays using NIH 3T3/Pdx1 and NIH 3T3/EV ... insulin promoter in the NIH 3T3/Pdx1 immunoprecipitation compared to NIH 3T3/EV immunoprecipitations. ...

Antibodies for Studying NMDA Receptor Protein Expression and Synapse-Specific Immunolabeling

... and Anti-NMDAR2B was established in immunoprecipitation and Western blot experiments of whole ... cross-reactivity was seen for the NR2B immunoprecipitation blotted with Anti-NMDAR2A. Anti-NMDAR2C ... for Western blot, immunohistochemistry and immunoprecipitation analyses. For added convenience, Stratagene ...

NimbleGen to be part of new cancer study

... (LICR) and Iceland Genomics Corp. on the research. In the first phase, NimbleGen and LICR researchers will collaborate on optimizing chromatin immunoprecipitation (ChIP)-chip methodologies to increase by threefold the number of samples that can be studied on a single microarray as well as reduce experimental ...

NimbleGen partners with leading researchers

... the door for organizations like Yale University , the University of California-Davis , and UC-San Diego to work with NimbleGen's chromatin immunoprecipitation (chIP) arrays, which offers increased probe density. What can be done with the 2.1 million probes contained in each microarray? Dr. Stan Rose, ...

Performance Comparison of the Experion Automated Electrophoresis System and a Competing Automated System for Protein Analysis, Rev A

... performing one-dimensional differential expression studies, degradation assessments, cleavage investigations, and analysis of immunoprecipitation experiments. This tech note presents a comparison of the performance of the Experion Pro260 analysis kit with the Agilent ...

Preparative Nondenaturing Gel Electrophoresis to Purify NADP-Specific Glutamate Dehydrogenase From Chlorella, Rev B

... of an ammonium-inducible glutamate dehydrogenase and the use of its antigen affinity column-purified antibody in specific immunoprecipitation and immunoadsorption procedures, Anal Biochem 110, 216228 (1981) Diaflo is a trademark of W R Grace & Co. ...

High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli

... be of particular interest to scientists who wish to use calmodulin affinity-purified proteins to study protein-protein interactions by pull-down immunoprecipitation experiments using eukaryotic cell extracts, or who wish to probe lambda cDNA expression libraries for interacting proteins. In both cases, the ...

Epitope-Tagging Vectors for Functional Analysis in Yeast

... under the control of a repressible promoter, two different genes can be coexpressed in yeast, protein-protein interactions can be confirmed by immunoprecipitation analysis, and protein localization can be studied. Together with the use of other vectors, the pESC vectors can be used to characterize ...

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Immunoprecipitation in Biological Products

FLAG Immunoprecipitation Kit from Sigma-Aldrich

Description:... Sigmas FLAG immunoprecipitation kit allows a rapid and efficient immunoprecipitation and elution of an active FLAG-tagged protein. The immunoprecipitation is performed with ANTI-FLAG-M2 affinity gel, ...
Company:Sigma-Aldrich

Rabbit Anti-SKAP55 Polyclonal Antibody, Unconjugated from ABR-Affinity BioReagents

Description:... has been successfully used in Western blot, immunocytochemistry and immunoprecipitation procedures. By Western blot, this antibody detects an ~ 60 kDa protein from human Jurkat cells.This antibody has also been used in immunoprecipitation and immunocytochemical procedures. PA1-525 antigen corresponds to ...
Company:ABR-Affinity BioReagents

ChIP-PCR for NFkb from Aviva Systems Biology

Description:... the most important parameter. Two major problems concerning the subsequent immunoprecipitation step should be taken into account: first, an excess of crosslinking can ... known promoter regions can be performed to check whether the chromatin immunoprecipitation is ....
Company:Aviva Systems Biology

Catch and Release® v2.0
Reversible Immunoprecipitation System from Upstate

Description:... Catch and Release® v2.0 Reversible immunoprecipitation ...
Company:Upstate

Human / Mouse / Rat Chk1 Kinase, active ELISA development Kit, DuoSet, IC (Intracellular) from R&D Systems

Description:... For the development of an immunoprecipitation assay to measure Chk1 kinase activity in cell ....
Company:R&D Systems

Anti-Bunyavirus (G1) Monoclonal Antibody, Unconjugated, Clone 8C2.1 from CHEMICON

Description:... Monoclonal 8C2.1 is specific for Bunyavirus G1 as tested by neutralization, immunoprecipitation and immunobinding ....
Company:CHEMICON

Rabbit Anti-mTOR Monoclonal Antibody,, Clone ZMD.414 from Invitrogen

Description:... kDa. Reactivity: Human (positive control: HEK293 cell lysates). Based on sequence homology reactivity with mouse and rat is expected. Applications: immunoprecipitation Western ...
Company:Invitrogen

Mouse Anti-JAB1 (Mid) Monoclonal Antibody,, Clone 3F10B10 from Invitrogen

Description:... CSN5). Specificity: Specific for the JAB1 protein. Reactivity: Human (positive controls: 293T Jurkat cell lysates). Applications: Western blotting immunoprecipitation ...
Company:Invitrogen

Rabbit Anti-Human XAB2 (N-terminus) Polyclonal Antibody, Unconjugated from GeneTex

Description:... Rabbit polyclonal antibody to human XAB2 (amino terminus) XAB2 (XPA-binding protein 2) was first identified through its interaction with XPA. immunoprecipitation experiments have also demonstrated that a fraction of XAB2 interacts with the transcription-coupled repair-specific proteins CSA and CSB as well as ...
Company:GeneTex

EZ-Chip from Upstate

Description:... From the company that first brought you the chromatin immunoprecipitation (ChIP) kit, a new and improved kit, EZ-ChIP, is now available to make your experiments easier, quicker, and most importantly, more ....
Company:Upstate

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Immunoprecipitation in Biological Definition

Monoclonal antibody

... immunofluorescence test (to detect a substance in a whole cell). Monoclonal antibodies can also be used to purify a substance with techniques called immunoprecipitation and affinity chromatography . In medicinal treatments, the small variation (if any) in recognizing the antigen helps to reduce side ...
Immunoprecipitation in Biological Dictionary

Immunoprecipitation

... immunoprecipitation (IP) is the technique of precipitating a ... protein from a sample containing ... immunoprecipitation Precipitation resulting from interaction of antibody and soluble antigen. ... immunoprecipitation (IP) is the technique of precipitating a ... ...

Chromatin

... cell nucleus. ... MeSH Chromatin +immunoprecipitation ... Chromatin immunoprecipitation (ChIP) of Protein Complexes: Mapping of Genomic ... Chromatin immunoprecipitation ... Information about chromatin in the free online English ...

Genome

... genes and non-coding ... Genome -Wide Analysis of KAP1 Binding Suggests Autoregulation of KRAB-ZNFs. We performed a genome -scale chromatin immunoprecipitation (ChIP)-chip comparison ... ...

DNA methylation

... DNA that often causes changes in the ... The identification of DNA methylation patterns is a common procedure in the ... Methylated DNA immunoprecipitation (MeDIP) is an efficient technique for the ... DNA Methylation Assays and tools from Active Motif to study Epigenetics and Chromatin ...

Antibodies

... in flow cytometry, ... Biotinylation of antibodies ... Conjugation of monoclonal antibodies to fluorescent dyes - an in-depth guide to ... immunoprecipitation with soluble antibodies ... A researcher's guide to companies that sell antibodies and antibody related products ... Academy Biomed - ...
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