A Microtiter-based Assay for the Determination of ID50s of b-lactamase Inhibitors Employing Reporter Substrates Detected at UV or Visible Wavelengths (MaxLine Application Note #20)

... antibiotic imipenem (λ max = 299 nm) as reporter substrates. Imipenem was used for those carbapenemases which did not have significant hydrolytic activity against nitrocefin. The method described uses the UV capability of the SPECTRAmax 250 micro-plate spectrophotometer and can be readily ...

An Optimal DNA Microarray Substrate for the Identification of Fetal and Somatic Liver Stem Cells of the Rat

... primarily encode phase I and phase II enzymes and matrices. Oxidation, reduction, methylation and desulphurizing reactions, as well as hydrolytic cleavages occur in phase I of the liver metabolism. The biological function of these reactions is the transformation of toxic ...

Hydrolytic in Biological Products

Amylase Fluorometric (Green) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)

Description:... α-Amylase is a hydrolytic enzyme that catalyzes the conversion of starch to a mixture of glucose, maltose, maltotriose and dextrins. The level of a-amylase in various fluids ...
Company:Molecular Probes (Invitrogen)

AgarACE Enzyme from Promega

Description:... required to melt them may be deleterious to the DNA or RNA. Finally, AgarACE Enzyme does not require buffer exchange in order to exhibit high agarose hydrolytic activity; its activity does not appreciably change in TAE or TBE buffers used in normal electrophoretic ....
Company:Promega

Hydrolytic in Biological Definition

Hydrolytic enzyme

... hydrolytic enzymes break down protein, carbohydrate, and fat molecules into their simplest units. The hydrolysis of polymers by hydrolytic enzymes results in free monomers . During hydrolysis ( ...

Hydrolysis

... each product. Irreversibility of hydrolysis under physiological conditions Under physiological conditions (i.e. in dilute aqueous solution), a hydrolytic cleavage reaction, where the concentration of a metabolic precursor is low (on the order of 10 -3 to 10 -6 molar), is essentially thermodynamically ...

Hydrolytic in Biological Dictionary

Lysosome

... cytoplasm of most cells containing various hydrolytic enzymes that function in intracellular ... organelle of eukaryotic cells that contains hydrolytic enzymes ... 3D structures of proteins ... ... Lysosomes contain a wide variety of hydrolytic enzymes ... Brief and Straightforward Guide: ...

Lysosomes

... compartments containing a variety of hydrolytic enzymes. Lysosomes are the cells' garbage ... cytoplasm of most cells containing various hydrolytic enzymes that function in intracellular ... The lysosome is a membranous bag which contains hydrolytic enzymes that are used ... The lysosome ...

Hydrolytic enzyme

... the following reaction is a hydrolase: hydrolytic , enzyme ,biological ,biology dictionary,biology ... terms,biology abbreviations ... enclosed bag of hydrolytic enzymes found in the ... hydrolytic enzyme ( ¦hīdrə¦lidik ′en′zīm ) ( ...

Deoxyribonuclease

... A deoxyribonuclease is any enzyme that catalyzes the hydrolytic cleavage of phosphodiester linkages in the DNA backbone. Deoxyribonucleases are thus one type of nuclease. A wide variety of deoxyribonucleases ...

Deamination

... drive other vital processes. The first step in the mobilization of amino acids for energy production is deamination , the splitting off ... hydrolytic deamination of cytosines in DNA creates uracil and, if unrepaired, ... It decreases the rate of cytosine deamination to uracil by 7-fold. .....

Acrosome reaction

... pellucida-induced acrosome reaction of human spermatozoa is mediated by ... undergo the acrosome reaction resulting in the release of hydrolytic enzymes and ... factors in acrosome reaction were also used to determine which of them are ... Prostasome-like vesicles stimulate acrosome ...

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