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Exonuclease in Biological Technology

Using catalysts to stamp nanopatterns without ink

... on the stamp with a molecular tether of amino acids. For this proof-of-principle demonstration, Toones team chose as a catalyst the biological enzyme exonuclease I, derived from the bacterium E. coli. In one set of experiments, the polyacrylamide stamp pattern bearing the tethered enzymes was then pressed ...

Pwo DNA Polymerase

... DNA polymerase and possesses an 3'5' exonuclease activity also known as proofreading activity. The enzyme has no detectable 5'3' exonuclease activity. Pwo DNA polymerase exhibits ... 5 min at this temperature. The inherent 3'5' exonuclease proofreading activity Pwo results in ...

HotStarTaq PCR Handbook

... 5'3' exonuclease activity: Yes ... 3'5' exonuclease activity: No ... exonuclease activity assay: Linearized ...

TripleMaster PCR System

... avoid primer/template degradation by 35 exonuclease activity and unspecific primer extension ... avoid primer/template degradation by 35 exonuclease activity and non-specific primer extension ... not recommended due to the inherent 35 exonuclease activity. 11.9 Primer ...

TripleMaster PCR System

... avoid primer/template degradation by 35 exonuclease activity and unspecific primer extension ... avoid primer/template degradation by 35 exonuclease activity and non-specific primer extension ... not recommended due to the inherent 35 exonuclease activity. 11.9 Primer ...

Restriction Endonucleases Undergo Rigorous QC Testing to Ensure Optimal Performance

... is not introduced during packaging. Detecting exonuclease Contaminants The integrity of a restriction ... ends. Stratagene specifically assays for exonuclease activity by incubating an excess of restriction ... genomic E. coli DNA. Contaminating exonuclease is indicated if over 0.6% of the 3 ...

PCR Master

... It is a highly processive 5'3' DNA polymerase which lacks 3'5' exonuclease activity. Note: For additional convenience and reliable ... of amplification, because it is highly processive and free of exonuclease and nicking activities Increases sensitivity, because it ...

Taq DNA Polymerase

... It is a highly processive 5'3' DNA polymerase which lacks 3'5' exonuclease activity. Note: For additional convenience and ... of amplification, because it is highly processive and free of exonuclease and nicking activities Increases sensitivity, because it ...

Laser Capture Microdissection (LCM) for the Analysis of Macrophage Gene Expression from Atherosclerotic Lesions

... 5 and 3 ends with 6-carboxyfluorescein (6-FAM) reporter and tetra methyl-6-carboxyrhodamine (TAMRA) quencher, respectively, is hydrolyzed by the 5 exonuclease activity of Taq DNA polymerase, causing an increase in fluorescent signal that is measured in real-time after each cycle of PCR amplification. ...

The iCycler iQ Detection System for TaqMan Assays, Rev B

... of the polymerase chain reaction (PCR*). One popular probe strategy is the TaqMan assay (PE Biosystems), which capitalizes on the 5' exonuclease activity of Taq polymerase to cleave a labeled hybridization probe during the extension phase of PCR (Holland et al. 1991). In ...

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Exonuclease in Biological Products

Klenow Fragment, Exonuclease Minus from Promega

Description:... DNA Polymerase I Large (Klenow) Fragment, exonuclease Minus, is a DNA-dependent DNA polymerase that ... and the 3´→5´ exonuclease activities present in intact E. coli DNA ... displacement amplification (5). Klenow Fragment, exonuclease Minus, will leave a single-base 3´ overhang ...
Company:Promega

Exo- Klenow Fragment (Cloned) from Ambion

Description:... engineered Klenow polymerase in which the 3 to 5 exonuclease activity has been removed, leaving only the 5 to 3 polymerase activity. The elimination of exonuclease activity makes Exo- Klenow Fragment the enzyme of ... for nonspecific contaminating endonuclease, exonuclease and protease activity. Functionality is ...
Company:Ambion

DNA Exonuclease III , E. coli from EPICENTRE Biotechnologies

Description:... exonuclease III digests duplex DNA in a 3'-> 5' direction ... defined reaction conditions, DNA degradation by exonuclease III proceeds at a uniform rate yielding ... excision of deoxyribonucleotides by exonuclease III is dependent upon reaction factors including ...
Company:EPICENTRE Biotechnologies

NovaTaq DNA Polymerase from Novagen

Description:... NovaTaq DNA Polymerase possesses an integral 5′→3′ exonuclease and lacks 3′→5′ exonuclease activity. The enzyme generates PCR products with 3′-dA overhangs, ...
Company:Novagen

NovaTaq DNA Polymerase from Novagen

Description:... NovaTaq DNA Polymerase possesses an integral 5′→3′ exonuclease and lacks 3′→5′ exonuclease activity. The enzyme generates PCR products with 3′-dA overhangs, ...
Company:Novagen

MasterAmp Taq DNA Polymerase Kit from EPICENTRE Biotechnologies

Description:... for that purpose. The enzyme has an intrinsic 5'?3' structure-dependent exonuclease activity, but lacks 3'--5' proofreading exonuclease activity. MasterAmp Taq DNA Polymerase is highly purified using a ...
Company:EPICENTRE Biotechnologies

Red Taq DNA Polymerase from BullsEye from MIDSCI

Description:... Bullseye Red Taq DNA Polymerase has both a 5'3' DNA polymerase and a 5'3' exonuclease activity. The enzyme lacks a 3'5' exonuclease activity. Red Taq DNA Polymerase leaves an A overhang, which makes the ...
Company:MIDSCI

BioPrime Plus Array CGH Indirect Genomic Labeling Module from Invitrogen

Description:... large fragment of the DNA polymerase I holoenzyme that has both 5 and 35 exonuclease activity removed. The lack of exonuclease activity makes this enzyme ideal for use in random priming protocols, ...
Company:Invitrogen

DNA Polymerase I, E. coli from EPICENTRE Biotechnologies

Description:... DNA polymerase. The enzyme also contains both 5'--3'and 3'--5' exonuclease activities.1 The 5'?3' exonuclease activity enables the enzyme to use nicks and gaps in the DNA as starting ...
Company:EPICENTRE Biotechnologies

DNA Polymerase I from Promega

Description:... into duplex DNA in a 5?3 direction. DNA Polymerase I possesses a 3?5 exonuclease activity or "proofreading" function, which lowers the error rate during DNA replication, and also contains a 5?3 exonuclease activity, which enables the enzyme to replace nucleotides in the growing ...
Company:Promega

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Exonuclease in Biological Definition

Polymerase chain reaction

... Taq is that it sometimes makes mistakes when copying DNA, leading to mutations (errors) in the DNA sequence, since it lacks 3'->5' proofreading exonuclease activity. Polymerases such as Pwo or Pfu, obtained from Archaea , have proofreading mechanisms (mechanisms that check for errors) and can ...
Exonuclease in Biological Dictionary

Klenow Fragment

... I which retains polymerization and 3'-> 5' exonuclease activity, ... Klenow Fragment (3'-> 5' ... activity, but has lost the 5'-> 3' exonuclease activity and ... Klenow fragment ... " Klenow Fragment " Klenow Fragment , exonuclease Minus ... Enzymes " DNA Polymerases " Klenow ...

Exonuclease

... the 3’ or 5’ ends. Its close ... exonuclease activity. • protein binding. • hydrolase activity ... Human exonuclease 1 functionally complements its yeast homologues ... ... The European Bioinformatics Institute ... exonuclease , RNase T and DNA polymerase III. Example ...

Taq polymerase

... polymerase . ... 5-6 - Taq DNA Polymerase (temperature-dependent inhibitor), Vendor B ... Taq DNA Polymerase has no detectable 3'=>5' exonuclease (proofreading) activity ... The error rate of Taq DNA Polymerase in PCR is 2.2x10-5 errors per nt per cycle, ... Taq Polymerase . GENTAUR ...

Polymerase

... DNA Polymerase is a thermostable DNA polymerase that possesses a 5'->3' polymerase activity and a double-strand specific 5'->3' exonuclease activity. ... DyNAzyme EXT DNA Polymerase is a versatile and easy-to-use enzyme especially suited for handling demanding reaction conditions ...

PolyA tail

... digestion with nuclease and greatly ... Molecular dissection of mRNA poly(A ) tail length control in yeast. ... Poly(A ) Tail -dependent exonuclease AtRrp41p from Arabidopsis thaliana Rescues ... The Poly(A ) Tailing Kit uses E. coli poly(A ) polymerase I (E-PAP) to polyadenylate ... Effect ...

Okazaki fragments

... Reiji Okazaki . ... DNA polymerase and the fragments are joined by DNA ligase. ... Okazaki fragment , DNA polymerase I uses. its exonuclease ... discontinuous units called Okazaki fragments . The polymerase moves in the 5'Ë3' direction. ... Energetic and conformational ...

Nuclease

... relatively nonspecific ... nuclease -free ... The nuclease as a model for protein folding, Molec. Cell. Biochem., 23, ... Nuclease BAL-31 exonuclease degrades both 3' and 5' termini of duplex DNA ... Can Nuclease BAL-31 be used to remove 10 base pairs from the end of a DNA fragment? ... ...

Genomic clone

... . ... Is an international scientific electronic journal which publishes paper from all ... One of the genomic clone (pLK8) was subjected to exonuclease III deletion using ... Curso de Doctorado de Cartografía y análisis de genomas ... Session 1. Genomes and Genomic Cloning ... Screening of ...

AP endonuclease

... characterized AP endonuclease is, interestingly, the E. coli enzyme ... in vivo, but it is now clear that the AP endonuclease activity of exonuclease ... ...

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