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Ethidium in Medical Products

HE 33 Mini Submarine unit, Complete

Description:... 500 mland seal. Keep the base stored in the freezer. The UV transparent running tray can be placed on a transilluminator for viewing ethidium bromide stained bands. No taping required for casting. Small footprint13 cm wide x 24 cm long x 7 cm high. Electrical safety interlocks in the ...
Company:Hoefer, Inc.

HE 33 Mini Submarine unit, Basic

Description:... 500 mland seal. Keep the base stored in the freezer. The UV transparent running tray can be placed on a transilluminator for viewing ethidium bromide stained bands. No taping required for casting. Small footprint13 cm wide x 24 cm long x 7 cm high. Electrical safety interlocks in the ...
Company:Hoefer, Inc.

RF-Mini 150 Fluorometer

Description:... as 1ng/ml of DNA specific Hoechst Dye. By switching to another set of excitation and emission filters, the RF-Mini 150 can measure RNA and DNA with ethidium bromide, or measure cell viability with fluorescein based tags. When samples are not available in large quantity, a volume as small as 3L can be ...
Company:Shimadzu Scientific Instruments, Inc.

Mini ReadySub-Cell GT Cell

Features:... Tray locks into Sub-Cell GT cells Mini and wide mini formats Wide gel selection Three agarose percentages, TAE or TBE buffer, with or without ethidium bromide ...
Company:Bio-Rad Lab Equipment

Wide Mini ReadySub-Cell GT Cell

Features:... Tray locks into Sub-Cell GT cells Mini and wide mini formats Wide gel selection Three agarose percentages, TAE or TBE buffer, with or without ethidium bromide ...
Company:Bio-Rad Lab Equipment

OLERUP SSP™ HLA Kits

Features:... for HLA-A, HLA-B, and DRB. Complete line of accessory products for ease of use including a DNA size marker, negative control tubes, PCR wipe test, ethidium bromide dropper bottles and gel - electrophoresis equipment. Customized GenoVision version of HELMBERG-SCORE® Virtual Sequencing software ...
Company:QIAGEN
Ethidium in Biological News

Researchers create new class of fluorescent dyes to detect reactive oxygen species in vivo

... species, does not have. The dyes also have other advantages over DHE. "When DHE comes into contact with reactive oxygen species, it oxidizes into ethidium bromide, a common mutagen, which means it's toxic and can't be injected inside the body," explained Murthy. "DHE also auto-oxidizes in the presence ...
Ethidium in Biological Technology

Advantages of ReadyAgarose Precast Gels Over 96-Well Handcast Gels for High-Throughput Analysis, Rev A

... in TBE or TAE buffer, with or without ethidium bromide, to fit your research requirements. In ... to cool for 10 min, and 14.5 l of 10 mg/ml ethidium bromide was added. The molten agarose ... TAE 3% ReadyAgarose gels (with ethidium bromide) using a multichannel pipet. Several ...

Real-Time PCR/Melt-Curve Analysis: SNP Detection With FRET, Rev A

... but must be supplemented with ethidium bromide for use with FAM-Cy5, FAM-LC640, or ... pH 8, 50 mM KCl, 3 mM MgCl 2 ) with ethidium bromide (see below) in place of the 1x well ... For FAM-Cy5 filter combination, add 100 l of ethidium bromide (10 mg/ml stock) to 0.9 ml of ...

Detection of Mutations in the Fas Antigen of Lymphoma Tumors by RT-PCR and Denaturing Gradient Gel Electrophoresis

... hours. PCR products were detected by ethidium bromide staining. Results ... cDNA detectable (Figure 1). Using ethidium bromide staining, we were able to ... Figure 2 shows a representative ethidium stained DGGE gel including 10 patient ...

Isolate It All: siRNA miRNA Total RNA Native Protein

... acrylamide gel and stained with ethidium bromide. (B) Electropherogram of total RNA ... denaturing glyoxal agarose gel and stained with ethidium bromide. (B) 1 g of each fraction ... acrylamide gel and stained with ethidium bromide. After electrotransfer, miR-16 miRNA ...

Two-step Gradient PCR*

... Figure 1A. 1% Agarose gel stained with ethidium bromide. Lane 1, 1 kb DNA ... Figure 2A. 1% Agarose gel stained with ethidium bromide. Lane 1, 1 kb DNA ... Figure 3. 1% Agarose gel stained with ethidium bromide. Lane 1, 1 kb DNA ...

Eppendorf Perfectprep Gel Cleanup Kit

... 1x TBE (Eppendorf) ethidium Bromide (Sigma) Mastercycler gradient ... agarose gel containing 2 l of 10 mg/ml ethidium bromide. The gel was run in 1x TBE at 120 V for ... SeaKem LE agarose gel containing 2 ml of 10 mg/ml ethidium bromide. The gel was run in 1x TBE at ...

Eagle Eye Optical Filter Optimized for Imaging Silver-Stained Gels

... for imaging nucleic acid gels stained with ethidium bromide. Because the system is also frequently ... Stratagenes optical filter line now comprises ethidium bromide, SYBR Green, 2 Coomassie Blue, ... compare the performances of the silver-stain and ethidium bromide filters. A stained gel was first ...

Improved Sensitivity for Staining RNA with SYBR Gold Stain

... with a nucleic acid stain. Although ethidium bromide is commonly used to stain RNA gels, we found that staining with ethidium bromide often requires as much as 1 g of RNA to ... up to 48 hours. To compare SYBR Gold stain to ethidium bromide staining, two identical gels were run ...

Optimized Imaging of Protein Gels Stained with Coomassie Brilliant Blue Dye

... a single filter specifically designed for ethidium bromide may be inadequate for use with other ... for stained protein gels when compared to the ethidium bromide-optimized filter. Sensitivity was ... upon excitation with ultraviolet light. This ethidium bromide-optimized filter preferentially allows ...

Laser Capture Microdissection of muscle fiber populations and expression analysis by RT-PCR

... UltraPure TM 10x TAE (Invitrogen, Cat. # 15558-026) ◊ ethidium Bromide (Sigma, Cat. # E-1510) ◊ Tissue-Tek OCT ... agarose gel and separated by 120 V for 25 minutes. Gels are stained with ethidium bromide and visualized using the Gel Doc 2000 TM Gel Documentation ....

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Ethidium in Biological Products

GenElute Minus EtBr Spin Column from Sigma-Aldrich

Description:... provide a rapid and simple method for recovering ethidium bromide (EtBr)-free DNA (100 bp to 10 kb) from ... of membranes and filters that hold agarose and ethidium bromide back while allowing DNA to selectively ... of the agarose gel. More than 95% of the ethidium bromide is typically removed from the ....
Company:Sigma-Aldrich

UltraPure Agarose and SYBR Safe DNA Gel Stain combo from Invitrogen

Description:... Offers a safer and better alternative to ethidium bromide Eliminates risks to yourself, the ... mutagenicity, making it much safer to use than ethidium bromide. It is provided as a convenient ... SYBR Safe is comparable to that obtained with ethidium bromide. DNA bands stained with SYBR Safe DNA ...
Company:Invitrogen

UltraPure Agarose and SYBR Safe DNA Gel Stain Combo from Invitrogen

Description:... Offers a safer and better alternative to ethidium bromide Eliminates risks to yourself, the ... mutagenicity, making it much safer to use than ethidium bromide. It is provided as a convenient ... SYBR Safe is comparable to that obtained with ethidium bromide. DNA bands stained with SYBR Safe DNA ...
Company:Invitrogen

SYBR Safe DNA Gel Stain Starter Kit - with 1 L of SYBR Safe DNA gel stain in 0.5X TBE (S33100) and one photographic filter (S37100) from Molecular Probes (Invitrogen)

Description:... for reduced mutagenicity to be safer than ethidium bromide for staining DNA in agarose or acrylamide ... SYBR Safe stain is not only less mutagenic than ethidium bromide but SYBR Safe stain's detection sensitivity is better than that of ethidium bromide. SYBR Safe stain comes as a premixed ...
Company:Molecular Probes (Invitrogen)

DNA Visualizer Extraction Kit from Abnova Corporation

Description:... used for DNA-cloning work. The conventional method of visualizing DNA with ethidium bromide and exposure of UV light damages DNA and significantly decreases ... no UV damage, obtained DNA shows higher cloning efficiency than one using ethidium bromide and UV light. The Purified DNA with DNA Visualizer Extraction Kit ...
Company:Abnova Corporation

SYBR Safe photographic filter from Molecular Probes (Invitrogen)

Description:... for Fluorescent DyeStained Gels and Blots) is ideal for black-and-white photography of gels stained with the SYBR Safe DNA gel stain, the safer ethidium bromide alternative. Note that the SYBR Safe photographic filter is identical to the SYPRO photographic filter, which is described ....
Company:Molecular Probes (Invitrogen)

High Molecular Weight DNA Markers from Invitrogen

Description:... agarose gels. The 13 bands consist of 8.3- to 48.5-kb fragments generated from restriction endonuclease digests of Lambda DNA (cIind1ts857 Sam7). ethidium bromide staining causes the four bands identified by an asterisk (8271 bp 8612 bp 12220 bp and 19399 bp) to show greater intensity allowing them to ...
Company:Invitrogen

Supercoiled DNA Ladder from Invitrogen

Description:... Supercoiled DNA Ladder is suitable for sizing supercoiled DNA from 2 to 16 kb by agarose gel electrophoresis (1). The bands can be visualized by ethidium bromide staining and may also be detected on Southern blots by probing with the pBR322 Beta-lactamase (ampicillin-resistance) gene ....
Company:Invitrogen

EasyCount ViaSure Kit from Immunicon Corp.

Description:... dead and live nucleated cells automatically. The ViaSure Staining Reagent uses a mixture of ready-to-use nucleic acid dyes - Acridine Orange and ethidium Bromide to identify live and dead cells, respectively, in a single test, while the EasyCount Slide 6 Multi-well Slide provides the ability to ...
Company:Immunicon Corp.

dsRNA Marker from Abnova Corporation

Description:... The dsRNA Marker is manufactured for non-denaturing polyacrylamide gel electrophoresis. The dsRNA Marker can be visualized by UV light after ethidium bromide ....
Company:Abnova Corporation

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Ethidium in Biological Definition

Gel electrophoresis

... electrophoresis run, when the smallest molecules have almost reached the anode, the molecules in the gel can be stained to make them visible. ethidium bromide , Silver, or Coomassie blue dye can be used. Other methods can also be used to visualize the separation of the mixture's components on the ...

Gel electrophoresis

... electrophoresis run, when the smallest molecules have almost reached the anode, the molecules in the gel can be stained to make them visible. ethidium bromide , Silver, or Coomassie blue dye can be used. Other methods can also be used to visualize the separation of the mixture's components on the ...
Ethidium in Biological Dictionary

Ethidium Bromide

... ethidium bromide is an intercalating agent commonly used ... light, it will fluoresce with ... ethidium bromide ( e′thidēəm ′brō′mīd ) ( organic ... melting point of 238-240°C; used in treating ethidium bromide is a large, flat basic molecule that ...

Gel electrophoresis

... for ... Agarose gel electrophoresis Digital image of 3 plasmid restriction digests run on a 1% w/v agarose gel , 3 Volts/cm, stained with ethidium bromide Experiment 2: Gel Electrophoresis of DNA. What is Electrophoresis ? Electrophoresis is a technique used in the laboratory ... Let's ...

Agarose Gel

... ... Agarose gel electrophoresis Digital image of 3 plasmid restriction digests run on a 1% w/v agarose gel , 3 Volts/cm, stained with ethidium bromide The more agarose is dissolved in the boiling water, the firmer the gel will be. ... If you were inside an agarose gel , your ...

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