Lack of enzyme turns fat cells into fat burners
... the effectACC2 has on mice. Mice bred to lack the enzyme can eat a high fat, highcarbohydrate diet without ...intenance," said Wakil. "ACC2 is potentially a key enzyme inthe regulation of weight, obesity, and related problems."Wakil and his colleagues studied the oxid...Researchers Uncover Key Step In Manufacture of Memory Protein
A cellular enzyme appears to play a crucial role in the manufacture ... Specifically, the researchers discovered that the enzyme carboxypeptidase E, (CPE) is needed to deliver the early, or inactive, form of BDNF ?proBDNF ?to a s...Combination therapy boosts effectiveness of telomere-directed cancer cell death
...erase inhibition is combined with inhibition of an enzyme calledtankyrase 1 that is involved in making telomeres accessible totelomerase. The researchers found that both tankyrase 1 activity andtelomere shortening decrease the effects of telomerase inhibitors.Importantly, tankyrase inhibition enhanced telom...GATA: a graphic alignment tool for comparative sequence analysis
...erase inhibition is combined with inhibition of an enzyme calledtankyrase 1 that is involved in making telomeres accessible totelomerase. The researchers found that both tankyrase 1 activity andtelomere shortening decrease the effects of telomerase inhibitors.Importantly, tankyrase inhibition enhanced telom...Scientists find missing enzyme for tuberculosis iron scavenging pathway
... Baker and his colleagues have discovered that the enzyme most likely plays an entirely different role in M. tuberculosis. "What the structure showed, when combined with careful analysis of the sequence, its neighbors in the genome, and the fact that its gene was also regulated by iron, was that Rv1347c wa...Thermo Sequenase Dye Primer Manual Cycle Sequencing Kit from USB Corp.
...Thermo Sequenase DNA Polymerase/TAP Enzyme Mix The enzyme is a mixture of Thermo Sequenase and thermostable inorganic pyrophosphatase (TAP) cloned from the thermophile Thermoplasma acidophilum. TAP prevents pyrophosphorolysis from occurring. Pyrophosphorolysis can result in faint bands, which can affect the...Modern Protein Chemistry: Practical Aspects from Pierce Biotechnology, Inc.
...Cross-linking of membrane proteins Preparation of enzyme conjugates Preparation of immunotoxins and drug conjugates Coupling of proteins to solid supports Protein:DNA/RNA cross-linking...ALDEFLUOR, Stem Cell Identification, Kit from StemCell Technologies, Inc.
...oprietary technology comprises a substrate for the enzyme aldehyde dehydrogenase (ALDH) that is highly expressed in all viable stem cells, making ALDH an extremely useful marker for counting and isolating viable stem cells....Complete 2 channel polarographic oxygen monitoring system from Instech Solomon
...lic studies of mitochondria, cell suspensions, and enzyme assays....Hydrogen Peroxide / Peroxidase Fluorometric (resorufin) Assay Kit, Fluoro H2O2 from BACHEM
...n be utilized to detect H2O2 release from cells or enzyme coupled reactions, and it allows monitoring of additional parameters. The assay is adaptable for HTS format. Application: Fluorescence via microwell plate reader....New Mammalian Two-Hybrid System Detects Protein-Protein Interactions
...teraction is provided byan amino acid biosynthetic enzyme that is absent in the yeast host and whoseexpression becomes induced only when the transcription activator isreconstituted. This two-hybrid system has been widely used. Despite these successes, the Fields yeast two-hybrid system does notrepresent a ...Low Abundance cDNA Cloned Using Stratagenes Human Universal cDNA Library
... Although the catalytic subunit of the editing enzyme was discovered in 1993, the identity of th...our results support a model of the editing enzyme in which ACF is the RNA binding subunit that docks the catalytic subunit to edit the cytidin...Detect Single Nucleotide Sequence Changes with Mx4000 Molecular Beacon Allelic Discrimination Kits
... of activated protein C (APC), an anticoagulant enzyme G to A substitution that resul...rofolate reductase) A key enzyme in homocysteine metabolism C to T substitution at nucleotide position 677 ...Highest Possible Transformation Efficiencies for High-Throughput Applications
...e the superior choice for PCR cloning, restriction enzyme cloning, and ligase-independent cloning. In comparison transformations of either supercoiled or ligated DNA, 96Pack Gold chemically competent cells yielded higher efficiencies than another 96-well-format product. We confirmed ......l activity, and the tags do not interfere with the enzyme activity. We consistently observed that the luciferase activities derived from the pESC-URA vector are approximately two- to three-fold less, compared to those derived from other pESC vectors ( Figure 3 ). Lower levels...African HIV strains more resistant to anti HIV drugs
...difficult to latch on to the protease, which is an enzyme useful in the replication of the virus inside the body. If the drug attaches itself to the protease, then it is easier for the drug to deactivate the virus. This is difficult in two African strains called A and C subtypes of HIV, the researchers say...A ribozyme, an enzyme that can be used to kill the deadly Hepatitis E virus has been designed by Indian scientists.The ribozyme had been designed using the viral clones and would kill the virus by cutting RNA. The efficacy of the ribozyme was being tested on animal...Iron supplementation for a common side effect
... pressure medication. The angiotensin-converting enzyme (ACE) inhibitors are often used to treat high blood pressure, heart failure and other cardiac conditions. But up to 40% of people on ACE inhibitors are troubled by a cough. The side effect is not considered serious by doctors, but it is the most comm...... found a previously unknown chemical site on a key enzyme that regulates production of the genetic messenge...NA.In a series of complicated experiments with the enzyme from the bacteria E.Coli. Erie and her students found that when the precursor molecule does not occu......for patients suffering from angiotensin-converting enzyme (ACE) inhibitor-induced dry cough. Kyung Pyo Hong, MD, of the Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea, in his trial which included 19 subjects who had developed persistent dry cough while receiving ACE inhibi...Sensitive Eyes Enzymatic Cleaner
...utes. Sensitive eyes enzymatic cleaner contains an enzyme that breaks up cloudy, irritating film on soft contact lenses caused by protein build-up and tear deposits.......tomated analyzer designed to perform microparticle enzyme immunoassay (MEIA), fluorescence polarization immunoassay (FPIA) and ion capture technologies....CIC (C1-q) EIA Auto-Immune Markers 001-EK-CIC Circulating Immune Complexes
...ion of human IgG. After another washing step, the enzyme substrate is pipetted followed by a stopping step. The absorption is measured at 405 nm....Anti-MPO ANCA EIA Auto-Immune Markers 013-MPO-48 Myeloperoxidase
The Immunoscan MPO-ANCA Kit is an enzyme immunoassay (EIA) kit for the in vitro detection in human serum of the subclass of Anti-Neutrophil Cytoplasmic Antibodies (ANCA) directed against myeloperoxidase to be used as an aid in the diagnosis of crescentic glomerulonephritis.ANCA are a family...Retinol Binding Protein EIA Vitamin Assays 030-6110 RBP Bone and Minearl Metabolism
...tification of bound RBP is carriedout by adding an enzyme labeled antibody, which also binds to the RBP.The amount of converted substrate is directly proportional to the amountof bound RBP and can be determined photometrically at 450 nm (ifextinction is out of range, measure at 410 nm)....West Nile Virus Screening and Diagnostics Technology Emerges In Step with North American Outbreak
... conjugated with horseradish peroxidase (HRP), and enzyme substrate plus chromogen. Absorbance is measured ...y means of HRP-conjugated goat anti-human IgG, and enzyme substrate plus chromogen. Absorbance is measured at 450 nm. There are 3 wash steps and the total i...New Assays to Detect Disseminated Fungal Infections
...endent) pathway. The pathway activates a clotting enzyme that cleaves p -nitroaniline from a chromogenic peptide substrate. This in turn allows photometric readout. The entire test can be carried out with standard laboratory equipment. A related assay, Glucatell, is available for non-diagnostic purpose...New Point-of-Care Methods to Measure Coronary and Cerebrovascular Risk
...binds to skin cholesterol. The second contains an enzyme substrate that allows readout with a spectrophotometer. The test can be performed in minutes. PREVU(x) is marketed by McNeil Consumer Healthcare and approved for use in North America and Europe. The manufacturer has also announced plans for a qua......e wassimilar to enoxaparin. The incidence of liver enzyme elevations andacute coronary events during the tre...cifically and reversibly inhibits thrombin, thekey enzyme for blood clot formation, and is currently in phase 3development. It is administered as a once daily......reatic cancer to stably express firefly luciferace enzyme - thebioluminescent agent that lights up fireflies. This allowed liveimaging of the mice to monitor cancer progression. The researchers already had developed several components of thegene expression system. Led by first author Xiaoming Xie, Ph.D., a...