Endonuclease in Biological News
Scientists spy enzyme that makes us unique
... a process at the heart of human individuality.
The discovery of the T7 endonuclease
1 enzymes structure was made by using x-ray crystallography techniques. ... join together at random points in their sequence(1). Enzymes such as T7 endonuclease
1 are then responsible for severing the strands at this junction, thus ...
Just a little squeeze lets proteins assess DNA
Seven years ago, she figured out the structure of a protein called HincII that snips up DNA. The protein is a type of enzyme called a restriction endonuclease
and comes from Haemophilus influenzae bacteria.
Since that time, Horton has been trying to learn how HincII interrogates the DNA to find the ...
Removing DNA repair gene causes metabolic syndrome
... by their efforts to clone and crystallize homologs to the Escherichia coli endonuclease
VIII gene. E. coli endonuclease
VIII is part of a pathway of enzymes involved in repairing DNA damaged by ...
Scientists identify specific enzymes that make meningitis hard to fight
... therefore unaffected by the body's defences.
The research team found that the two enzymes essential to the bacteria's repair mechanism are AP endonuclease
and 3'-phosphodiesterase. Contrary to the scientists' expectations, these two enzymes carry out separate functions in the DNA repair process.
Endonuclease in Biological Technology
Frost & Sullivan Awards Precision Biosciences for Technology Innovation and Molecular Design Process in the Genomics Market
... in order to identify best practices in the industry.
About Precision Biosciences
Precision's mission is to utilize its engineered endonuclease
technology to become the world leader in the field of genomic molecular biology. Precision's proprietary Directed Nuclease Editor(TM) (DNE) ...
Polycystic Kidney Disease Assay at Cornell University Uses Transgenomic's Surveyor Nuclease & WAVE Technology
... that we have invested in these technologies."
Transgenomic's SURVEYOR Nuclease is a proprietary mismatch-specific endonuclease
that efficiently detects any mismatches in double-stranded DNA and cleaves at the site of DNA mutations. It identifies all base substitutions, ...
Alfacell's ONCONASE(R) and R-Amphinase Show Anti-Tumor Activity in Chronic Lymphocytic Leukemia and Acute Myeloblastic Leukemia Cells
... effect of ONCONASE and R-Amph was evident after 48 and 72 hours of treatment, respectively. Synergistic activity was seen in the combination of the endonuclease
plus either purine analogue. The combination of doxorubicin with ONCONASE or R-Amph demonstrated an increase in pro-apoptotic activity when compared ...
Transgenomic Awarded SBIR Grant to Support Development of SURVEYOR(R) Endonuclease Adaptor-ligated Libraries (SEAL) for Determining Whole Genome Sequence Variation
... genome analysis for pharmacogenomic studies in personalized medicine and
bacterial drug resistance research."
Adaptor-ligated Libraries (SEAL) is a cost-effective
and high throughput enabling technology for whole genome analysis under
development by ...
Transgenomic Introduces SURVEYOR(R) Endonuclease Adaptor-Ligated Libraries (SEAL) for Determining Whole Genome Sequence Variation
... identify point mutations and
small insertion/deletions at 100 to 1000 times lower sequencing intensity
than current methods."
Adaptor-ligated Libraries (SEAL) is a
cost-effective and high throughput enabling technology for whole genome
analysis under development by ...
Restriction Endonucleases Undergo Rigorous QC Testing to Ensure Optimal
... sample from each packaging of a
is confirmed prior to shipment to demonstrate ... preparation is free from contaminating endonuclease
activities, the overdigested ... activity by incubating an excess
of restriction endonuclease
for 4 hours with a mixture of single- ...
HotStarTaq PCR Handbook
... indicated under Exo.
Plasmid DNA is incubated with HotStarTaq DNA Polymerase
in PCR Buffer. endonuclease
activity per unit
of enzyme is indicated under ...
GenomiPhi amplification of FFPE tissue DNA for array CGH
... PicoGreen™ dsDNA Assay Kit (Invitrogen)
HyperLadder I DNA ladder (BioLine)
Dpn II restriction endonuclease
(New England BioLabs)
DNA extraction from FFPE tissue slides
Genomic DNA was isolated ...
Detection of Apolipoprotein Gene Variants by DGGE Using the DCode System
... using a temperature
cycler (Ericomp Inc., San Diego, CA). Both oligonucleotide primers had
GC clamps. 2 Following restriction endonuclease
digestion, the samples
were subjected to DGGE.
The amplification reactions were performed in 50 mM Tris-HCl, pH 9 (at
Sub-Micron Gold Particles Are Superior to Larger Particles for Efficient Biolistic Transformation of Organelles and Some Cells
... Zimmerly, S., Guo, H., Eskes, R., Yang, J., Perlman, P. S. and Lambowitz,
A. M., A group II intron RNA is a catalytic component of a DNA endonuclease
involved in intron mobility, Cell, 83 , 529-538 (1995).
Biolistic is a registered trademark of E.I.DuPont deNemours ...
Endonuclease in Biological Products
RNase Inhibitor from Applied Biosystems
Description:... enzyme used to inhibit RNase activity. It does not contain DNase or endonuclease
Inhibits RNase activity, preventing degradation of RNA template
Lacks DNA endonuclease
activity for better product yield ...
Gelzyme enzyme from Invitrogen
Description:... remaining carbohydrate molecules will not interfere with subsequent DNA manipulation. DNA isolated with Gelzyme enzyme can be used for restriction endonuclease
digestions ligations and transformations. Gelzyme enzyme is ideal for isolating DNA from low melting temperature agarose gels. It is provided with ...
High Molecular Weight DNA Markers from Invitrogen
Description:... double-stranded DNA from 9 to 48 kb on low-percentage agarose gels. The 13 bands consist of 8.3- to 48.5-kb fragments generated from restriction endonuclease
digests of Lambda DNA (cIind1ts857 Sam7). Ethidium bromide staining causes the four bands identified by an asterisk (8271 bp 8612 bp 12220 bp and ...
Plant DNAzol Reagent from Invitrogen
Description:... is specifically formulated for the isolation of genomic DNA from plants. Genomic DNA isolated with DNAzol Reagents can be used for restriction endonuclease
digestion Southern blot analysis molecular cloning and PCR ....
Topoisomerase I (Vaccinia) from Molecular Cloning Laboratories (MCLAB)
Description:... degree of supercoiling of naturally occurring DNA
Detecting mutant plasmids that differ in length by only one base-pair
Increasing restriction endonuclease
digestion of resistant DNA substrates by "unwinding" the DNA coils to expose restriction sites ...
Company:Molecular Cloning Laboratories (MCLAB)
GenElute Agarose Spin Columns from Sigma-Aldrich
Description:... The maximum gel slice weight is approximately 200 mg. The purified DNA can be used in most downstream applications such as ligation, PCR, restriction endonuclease
digestion, labeling and ....
DNA Exonuclease III , E. coli from EPICENTRE Biotechnologies
Description:... more in length, single-stranded DNA, or on thioester-linked nucleotides. The enzyme also has intrinsic RNase H, 3'-DNA phosphatase, and apurinic DNA endonuclease
Taq DNA Polymerase, 500 U (2 x 250 U) without dNTP from Bioneer, Inc.
Description:... in the amplification and sequence testing of DNA through PCR. The quality of the Taq DNA polymerase has been tested by the Activity Test, SDS-Page, endonuclease
Test, and ....
DNase I (RNase-free) (2 U/l) from Ambion
Description:... DNase I (RNase-free) (E.C. 126.96.36.199) is a nonspecific endonuclease
that degrades double-and single-stranded DNA and chromatin. It functions by hydrolyzing phosphodiester linkages, producing mono and oligonucleotides ...
EZ-96 DNA Methylation Kit - Deep Well from Zymo Research
Description:... l provided elution buffer.
*Input DNA: 500 pg - 2 g DNA per treatment with 200 - 500 ng being optimal.
*Sample Sources: Plasmid, genomic, and endonuclease
*Equipment: Microcentrifuge (EZ DNA Methylation Kit) centrifuge w/ microplate carriers (EZ-96 DNA Methylation ....
Endonuclease in Biological Definition
... A restriction enzyme (or restriction endonuclease
) is an enzyme that cuts double-stranded DNA . The enzyme makes two incisions, one through each of the phosphate backbones of the double helix ...
Endonuclease in Biological Dictionary
... Thus, this intein is likely to have lost its endonuclease
domain at ... CADs have a pair of motifs ... as well as detailed data for ... The homing endonuclease
domain of the HaV intein alleles is mostly ... positive genetic selection system ... Homing endonuclease
activity can mobilize an intein ... for loss ...
... 1988 Oct 7;55(1):79–89. ... protein is an endonuclease
that resolves the Holliday structure . ... Escherichia coli RuvC protein is an endonuclease
that resolves the Holliday structure . ... ... structure -specific recognition, ... T4 endonuclease
VII cleaves Holliday structures in vitro ...
... of simian virus 40 DNA by restriction endonuclease
of Hemophilus influenzae" ... new site-specific endonuclease
StuI from Streptomyces ... What is endonuclease
? Meaning of ...
... Apurinic/apyrimidinic ( AP ) endonuclease
is an enzyme that is involved in the DNA base ... the genome. The related ... AP , endonuclease
,biological ,biology dictionary,biology terminology,biology terms,biology abbreviations ... An endonuclease
which recognizes an AP site and cuts ... ...
... X06403. ... ... a textual map showing the positions of restriction endonuclease
cut sites. ... Restriction Map supports the entire IUPAC alphabet and several genetic codes. ... Restriction mapping . The endonuclease
Ceu-I recognizes a 19 bp sequence. ... Draw a restriction map of the ...
... S1 nuclease is an endonuclease
that is active against single-stranded DNA and RNA molecules. It is five times more active on DNA than RNA. Its reaction products are ...
... cerevisiae are single-celled fungi which that ... Saccharomyces cerevisiae cdc9, a structural gene for yeast DNA ligase which ... endonuclease
alpha from Saccharomyces cerevisiae shows increased activity on ... synteny blocks of current Saccharomyces cerevisiae genes. We ... ...
... be divided into endoribonucleases and exoribonucleases, and comprise several sub-classes within the EC ... Ribonuclease A (RNase A) is an endonuclease
that cleaves single-stranded RNA. Bovine pancreatic RNase A is one of the classic model systems of protein science. Ribonuclease summary with ...
... LINE-1 retrotransposon induces DNA damage and apoptosis in cancer cells ... Jr, Boeke JD: Human L1 retrotransposon encodes a conserved endonuclease
required ... inferred from 33 non-LTR retrotransposon clones. ... retrotransposon elements should be further investigated ... Morrison HG ...
... cleave foreign DNA, thus eliminating ... Restriction enzymes can be isolated from bacterial cells and used in the ... Finds restriction endonuclease
cleavage sites in DNA sequences. ... Maps sites for restriction enzymes , a.k.a. restriction endonucleases, in DNA sequences. ... With ...