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Denature in Medical Products

GenCatch™ Viral RNA Mini-Prep Kit

Description:... nucleic acids from cell-free samples. This system utilizes guanidine thiocyanate and 2-mercaptoethanol for their capabilities to inactivate RNase and denature proteins. Purified viral RNA is suitable fro applications such as RT-PCR, Northern and RNase protection assays. This system is ideal for isolation of ...
Company:Epoch Biolabs, Inc
Denature in Medical Definition

Artificial induction of immunity

... less than is required by the immune system to recognize the toxin and produce antibodies against it. However, heating the tetanus toxin enough to denature it causes it to lose its ability to produce disease, but still leaves it able to induce immunity to tetanus when injected into subjects. The heated, ...
Denature in Biological News

Ben-Gurion University engineers develop technique to help combat nuclear proliferation

... explains Ronen. "They wouldn't be able to get new plutonium for weapons if it is denatured, but countries that make nuclear fuel could decide not to denature it for themselves." Nuclear fuel used in nuclear reactors has two isotopes of uranium. One is fissionable, while the other is not. The ...

The Clorox Company's new indoor allergen product reduces common indoor allergens up to 90 percent

... the population is allergic to cats and dogs and 10 percent is allergic to dust mites. Clorox® Anywhere® Anti-Allergen Fabric Spray works to denature allergens produced from these sources as they make themselves at home in fabrics throughout the house. Keeps Fabrics Safe and Sound Allergens ...

A salty tale: New bacterial genome sequenced from ancient salterns

... do what it takes to survive, including exchanging genes with other microbial species that we might not expect." Normally, high-salt environments denature bacterial proteins. To survive, S. ruber must maintain a high concentration of potassium inside its cytoplasm, keeping an osmotic balance against the ...
Denature in Biological Technology

Probiotic Delivery Technology Awarded Third International Patent

... organisms through the manufacturing process -- Survival on the store shelf -- Safe passage through the harsh stomach acids that may denature the organisms BIO-tract products incorporate proven processes and technologies to overcome these barriers. How BIO-tract ...

Scalable Automation of the TempliPhi DNA Sequencing Template Amplification and DYEnamic ET Terminator Sequencing Kits on the Sciclone ALH 3000

... A1 is a tipbox, A2 is tip disposal, B2 is the denature buffer reservoir, C2 is the TempliPhi reaction ... of PCR wells, when dispensing the 10 μL of denature buffer, we dispensed 5 μL of the volume very ... Reservoir: The type of reservoir for holding denature buffer simply needs to be sufficient to hold the ...

Thermal Cycling Profile for Standard PCR

... Initial denaturation It is very important to denature the template DNA completely. Initial heating of the PCR mixture for 2 minutes at 9495C is enough to completely denature complex genomic DNA so that the primers can anneal to the template ...

Trouble-shooting: No product

... denaturation temperature to 95-97C denature DNA minus enzyme & buffer for 4-6 minutes ... denaturation temperature to 95-97C denature DNA minus enzyme & buffer for 4-6 minutes ...

Trouble-shooting: Misincorporation or low fidelity

... denaturation temperature to 95-97C denature DNA minus enzyme & buffer for 4-6 minutes ... Check integrity of template DNA Reduce cycling denature time to 15 - 30 seconds

GenomiPhi: protocol for BAC amplification and fabrication of slides for array CGH

... mixture was briefly centrifuged and then incubated at 30 ºC for 16-18 h. The reaction was stopped by heating at 65 ºC for 10 min to denature the Phi29 enzyme. At this point, the reactions can be kept on ice or stored at -20 ºC until needed. Note that reaction volumes can be increased ...

Oragene and Whole Genome Amplification with GenomiPhi

... provided with the GenomiPhi kit, 1 L of DNA was added to 9 L of sample buffer containing random hexamers and heated to 95C to denature the DNA. The sample was cooled and mixed with 9 L of reaction buffer containing salts and deoxynucleotides and 1 L of enzyme ...

Detection of Apolipoprotein Gene Variants by DGGE Using the DCode System

... and 72 C for 60 s. A final elongation step of 8 minutes at 72 C was then carried out. The temperature cycler was programmed to denature the amplification products at 96 C for 8 minutes and to then perform slow cooling to 40 C. Without this last step, heteroduplex ...

Detection of Mutations in the CYP21 Gene Using the DCode System

... The mutant PCR product was mixed with the corresponding wild type PCR product; the mixture was placed at 96 C for 10 minutes to denature the PCR products and subsequently left at room temperature for gradual renaturation, thereby generating heteroduplex molecules. ...

Temporal Temperature Gradient Electrophoresis: A Powerful Technique to Screen Mutations

... is decreased as DNA denaturation begins. Mutant and wild-type molecules have different melting behavior and thus begin to denature at different points in time resulting in a separation on the gel. The DCode system performs TTGE by controlling the ...

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Denature in Biological Definition

Macromolecule

... molecules is the need for assistance in dissolving into solution. Many require salts or particular ions to dissolve in water. Proteins will denature if the solute concentration of their solution is too high or too low. According to IUPAC recommendations the term macromolecule is reserved for ...
Denature in Biological Dictionary

Denaturing Gel

... instance, gels used in SDS-PAGE will unfold and denature the native structure of a protein. In contrast ... electrophoresisis desirable since it helps to denature the ... ... denaturing acrylamide gel of ... is desirable since it helps to denature the sample, ... DGGE Denaturing gradient ...

Denature

... the free encyclopedia (Redirected from denature ) ... Denatured alcohol, also known as methylated spirit ... denature tr.v. , -tured , -turing , -tures . To change ... of. ... At what temperature does amylase denature ? ... ...

SDS-PAGE

... using various types of gel. ... In SDS -polyacrylamide gel electrophoresis ( SDS - PAGE ), proteins are separated ... SDS does however denature the protein, so activity stains cannot be used to ... SDS - PAGE . Two-dimensional gel electrophoresis. Temperature gradient gel ...

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