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Denaturation in Medical Products

ThermoBrite

Description:... This programmable system automates the denaturation and hybridization steps in slide-based (F)ISH procedures, and provides walk-away convenience for clinical and research personnel. The low cost unit ...
Company:Iris Sample Processing (formerly StatSpin, Inc.)

TPO (Ab) RIA Thyroid Function 014-HD-90.1 Thyroid Peroxidase

Description:... of the TPO. The anti- gen TPO used in this competitive assay is indirectly labelled with a monoclonal iodine-125 antibody to prevent the denaturation of the sensitive antigen. In connection with the monoclonal antibody on the solid phase this method guarantees a high sensitivity and ...
Company:ALPCO Diagnostics

Vysis HYBrite

Features:... Eliminates the need for separate sample denaturation - probe and target DNA are co-denatured together Supports a variety of specimen types - assay parameters are supplied for Vysis CEP, LSI and WCP ...
Company:Abbott Diagnostics
Denaturation in Biological Technology

Noridian Medicare Now Covers Renessa(R) Treatment for Incontinence in Women

... of the female bladder neck and proximal urethra for stress urinary incontinence. This includes the Renessa transurethral collagen radiofrequency denaturation procedure." The website goes on to state that "coverage will be allowed when the procedure is performed consistent with FDA labeling and in ...

REPLI-g UltraFast Mini Kit

... methods. In addition, a unique alkaline denaturation buffer guarantees gentle denaturation of genomic DNA and avoids fragmentation of template DNA caused by heat denaturation methods. This ensures more sensitive and ...

Identification of Nonspecific Products Using Melt-Curve Analysis on the iCycler iQ Detection System, Rev A

... to a PCR amplification protocol of 95C denaturation for 3 min followed by 4045 cycles of ... under the following conditions: 1 min denaturation at 95C, 1 min annealing at 55C, 80 cycles of 0.5C ... The amplification steps were 95C denaturation for 3 min, 50 cycles of 10 sec at 95C, ...

Detection of Genetically Modified Soybean in Processed Foods Using Real-Time Quantitative PCR with SYBR Green I Dye on the DNA Engine Opticon 2 System

... 5 For both primer sets, the optimal denaturation and annealing temperatures were determined from denaturation and annealing gradient reactions (data ... the formula 10 (log%GMO) . Thermal denaturation profiles of all reactions were examined to verify ...

Separation of 16S rRNA PCR Products From a Model Community Using Temporal Temperature Gradient Gel Electrophoresis, Rev A

... followed by 35 cycles consisting of denaturation (60 seconds at 94 C), annealing (60 seconds at 55 ... for 17.5 hours with a temperature denaturation range of 23.1 C to 30 C and a ... weight and also the unique thermal denaturation characteristics of each PCR product ...

Northern Analysis: The Basics

... solution for sample denaturation. Sample denaturation in glyoxal/DMSO instead of ... incubation time for sample denaturation has been reduced, and no ... The volume ratio of glyoxal denaturation solution to sample RNA is ...

Thermal Cycling Profile for Standard PCR

... Initial denaturation It is very important to ... results. denaturation step during cycling denaturation at 9495C for 2030 seconds is usually sufficient, ...

Trouble-shooting: No product

... Incomplete template denaturation ... Incomplete template denaturation Insufficient heating during the denaturation step is a common cause of failure in a ...

PCR Optimization

... 12 different annealing, elongation, or denaturation temperatures. During the same run a number of ... also be used for optimization of the denaturation step, or the elongation temperature. DNA denaturation is a critical step in the PCR process ...

Optimizing DNA Amplification Protocols using the Eppendorf Mastercycler

... the reaction mixture after an initial denaturation step at 95C. This step is followed by ... denaturation In general, an initial denaturation process of a few minutes duration is ...

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Denaturation in Biological Products

SpotLight CISH Hybridizer (120 VAC, 50-60 Hz) from Invitrogen

Description:... x W x H): 17 3/4" x 8 15/16" x 5 5/16"18.7 lbsCapacity: 12 slidesTime: Programmable 0 - 100 hoursControl: Programmable 30 - 99C Automated denaturation and hybridization steps 40 user programmable protocols 3 operating modes (Denaturation/Hybridization, Hybridization only, and Fixed temperature) ...
Company:Invitrogen

XpressPack™ Luminescent Amplification Detection System, required for luminescent XpressPack™ from CHEMICON

Description:... a microplate luminometer; the intensity of the light is directly proportional to the amount of amplicon present in the sample. Components: denaturation Solution Hybridization Buffer AquaLite® Wash Buffer, 10X AquaLite® Streptavidin Assay Buffer, 10X AquaLite® Reconstitution Buffer ...
Company:CHEMICON

XpressPack™ Colorimetric Amplification Detection System, required for colorimetric XpressPack™ from CHEMICON

Description:... using a microplate reader; the intensity of the color is directly proportional to the amount of amplicon present in the sample. Components: denaturation Solution Hybridization Buffer Wash Buffer, 10X Strep:HRP Conjugate - Ready to Use Substrate (TMB/E) Stop Solution Applications: DNA ...
Company:CHEMICON

ThermoBrite (120 VAC) from StatSpin

Description:... This programmable system automates the denaturation and hybridization steps in slide-based (F)ISH procedures, and provides walk-away convenience for clinical and research personnel. The low cost unit ...
Company:StatSpin

EZ-96 DNA Methylation Kit - Deep Well from Zymo Research

Description:... PCR (MSP) followed by methylation analysis using restriction endonucleases, sequencing, microarrays, etc. SPECIFICATIONS *Format: Chemical denaturation of DNA followed by sodium bisulfite modification, Spin Column. *Recovery Volume: Purified, bisulfite treated DNA is recovered in less than or equal ...
Company:Zymo Research
Denaturation in Biological Definition

Denaturation

... denaturation in Biochemistry refers to a structural change ... caused by extreme conditions. denaturation can refer to the intentional adulteration of ... so that it is unsuitable as a beverage. denaturation of fissile materials is the process of ...
Denaturation in Biological Dictionary

Denature

... to (fissionable material) so as to make ... denaturation – Encyclopedia.com has denaturation articles, denaturation pictures, video and information ... Factors other ...

Denaturation

... denaturation is a process in which proteins or nucleic ... base, a concentrated inorganic salt, ... denaturation (biochemistry), a structural change in macromolecules caused by extreme conditions ... denaturation (fissile materials), transforming fissile ... ...

Ribonuclease

... cleavage of the phosphodiester bond ... Ribonuclease A has a molecular weight of 13,700 daltons. ... Ahmad, F.: Free Energy Changes in denaturation of Ribonuclease A by Mixed Denaturants. ... Function of Pancreatic Ribonuclease , Nature 221, 340, 1969 ... Online Shopping at SpotCost.com - ...

Mispairing

... Mutation process which occurs during DNA replication. It involves denaturation and displacement of the DNA strands, resulting in mispairing of the complementary bases. Slipped strand mispairing is one explanation for the ...

Lysis

... dictionary. ... Cell lysis and Lowry assay for 2D electrophoresis ... As soon as lysis occurs, proteolysis, dephosphorylation and denaturation begin to occur. ... 20 min is a traditional interval for cell lysis . ... Online Shopping at DirectTextBook.com - Save up to 80%, buy or rent. ...

Cooperativity

... depends on changes in dimer–dimer ... cooperativity , but discovered that upon ... Most importantly, however, cooperativity of DNA denaturation causes this ... We also demonstrate the extent of cooperativity , and find that the melting map ... ...

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