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Cosmid in Biological Technology

Efficient Cloning and Electro-transformation of Large Eukaryotic DNA Fragments

... in conjunction with the Human Genome Project. cosmid vectors are constrained to 4045 kb by ... Six plasmids were used: pUC19 (2.6 kb), 7 cosmid tsA/Y/1d4 (44 kb), 8 and F factor ... deletions in recombinant cosmid DNA propagated in Escherichia coli, J. ...

Isolation of Cosmids using Eppendorf's Perfectprep Plasmid Mini Kit

... a rapid and effective way of purifying cosmid DNA from bacterial culture. ... ranging from 30kb and 45kb DNA [1]. cosmid structures were created from a hybrid of ... to find a method that will purify the cosmid without shearing or excessive loss of DNA. ...

Easy Cosmid DNA Purification with StrataPrep Kits

... easily purify very large plasmids, including cosmid DNA. To demonstrate cosmid purification, a SuperCos I clone containing a ... using the StrataPrep EF plasmid midiprep kit. The cosmid DNA yield from the two cultures was 45 ...

Plasmid Miniprep Kit Selection

... For high- and low-copy plasmid and cosmid DNA preparations of * Option to automate For use on robotic workstations ...

QIAGEN Plasmid Kits

... For purification of up to 10 mg ultrapure plasmid or cosmid DNA Classic QIAGEN Plasmid Kits use gravity-flow anion-exchange tips for efficient purification of plasmid DNA ...

QIAfilter Plasmid Kits

... For fast purification of up to 10 mg ultrapure plasmid or cosmid DNA Features and benefits Up to 10 mg of ultrapure plasmid DNA Cleared lysates from up to ...

QIAGEN Plasmid Kits

... For purification of up to 10 mg ultrapure plasmid or cosmid DNA Classic QIAGEN Plasmid Kits use gravity-flow anion-exchange tips for efficient purification of plasmid DNA ...

EndoFree Plasmid Kits

... For purification of endotoxin-free ultrapure plasmid or cosmid DNA Features and benefits Less than 0.1 EU/g plasmid DNA Yields of up to 10 mg plasmid DNA ...

HiSpeed Plasmid Kits

... For ultrafast purification of up to 750 g plasmid or cosmid DNA Features and benefits Less than 45 min (Midi) or 60 min (Maxi) from harvested culture to ...

Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications and Agarose Gels

... 1.5- to 3-ml liquid cultures, purified plasmid DNA can be obtained in an average of 2.5 minutes per miniprep. Plasmid and cosmid DNAs isolated with the StrataPrep plasmid miniprep kit are easily digested with restriction enzymes ( figure 2 ). ...

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Cosmid in Biological Products

pWEB::TNC Deletion Cosmid Transposition Kit from EPICENTRE Biotechnologies

Description:... The pWEB::TNC™ Deletion cosmid Cloning Kit enables you to prepare, in about two days, a complete and unbiased primary cosmid library of genomic DNA for subsequent use in making nested deletion sublibraries. High efficiency cosmid library construction is accomplished using easy ...
Company:EPICENTRE Biotechnologies

pWEB Cosmid Cloning Kit from EPICENTRE Biotechnologies

Description: ...
Company:EPICENTRE Biotechnologies
Cosmid in Biological Dictionary

Cosmid

... A cosmid , first described by Collins and Hohn in 1978, is ... can be used to build genomic libraries. cosmid n. Genetics. A hybrid vector that has been ... large ... · plasmid · lambda phage · cosmid · P1 phage · fosmid · BAC ... Information ...

Overlapping clones

... because one of its end se ... To generate contigs, overlapping cosmid clones were identified primarily by ... If two cosmid clones are overlapping , the common bands produced on complete ... ....

Genomic library

... fragments that have ... ... received amplified aliquots of two genomic libraries for Aspergillus fumigatus. ... pWE15 and pLORIST2 genomic cosmid libraries of A. nidulans ... Second, we digest the genomic DNA of an organism that can metabolize lactose ... this strategy to make a ...

Contig map

... Wheat Contig Maps by Searching WheatDB ... Contig Assembly: FPC v6.0 is used to assembly contigs. ... A physical map is the ordering of cosmid clones by their position along ... A. nidulans mapping project, a contig map of P. carinii should ... of contig maps . ... Contig ...

Contig

... 3 dice as the starter square. ... on the Contig board. Put an X on this number. ... Contig Game. tkawas@mathwire.com ... The more detailed cosmid contig map depicts the order of overlapping DNA frag ... between fragments than contig maps (see below) ... Contig construction can be ... ...

Chromosome jumping

... applied the technique of chromosome jumping to this region. ... of a general human chromosome jumping library, with application to ... cosmid walking and chromosome jumping in the region of PKD1 reveal a locus ... A third CpG island was detected and cloned by directional chromosome ...

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