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Cosmid in Biological Technology

Efficient Cloning and Electro-transformation of Large Eukaryotic DNA Fragments

... in conjunction with the Human Genome Project. cosmid vectors are constrained to 4045 kb by ... Six plasmids were used: pUC19 (2.6 kb), 7 cosmid tsA/Y/1d4 (44 kb), 8 and F factor ... deletions in recombinant cosmid DNA propagated in Escherichia coli, J. ...

Isolation of Cosmids using Eppendorf's Perfectprep Plasmid Mini Kit

... a rapid and effective way of purifying cosmid DNA from bacterial culture. ... ranging from 30kb and 45kb DNA [1]. cosmid structures were created from a hybrid of ... to find a method that will purify the cosmid without shearing or excessive loss of DNA. ...

Easy Cosmid DNA Purification with StrataPrep Kits

... easily purify very large plasmids, including cosmid DNA. To demonstrate cosmid purification, a SuperCos I clone containing a ... using the StrataPrep EF plasmid midiprep kit. The cosmid DNA yield from the two cultures was 45 ...

Plasmid Miniprep Kit Selection

... For high- and low-copy plasmid and cosmid DNA preparations of * Option to automate For use on robotic workstations ...

QIAGEN Plasmid Kits

... For purification of up to 10 mg ultrapure plasmid or cosmid DNA Classic QIAGEN Plasmid Kits use gravity-flow anion-exchange tips for efficient purification of plasmid DNA ...

QIAfilter Plasmid Kits

... For fast purification of up to 10 mg ultrapure plasmid or cosmid DNA Features and benefits Up to 10 mg of ultrapure plasmid DNA Cleared lysates from up to ...

QIAGEN Plasmid Kits

... For purification of up to 10 mg ultrapure plasmid or cosmid DNA Classic QIAGEN Plasmid Kits use gravity-flow anion-exchange tips for efficient purification of plasmid DNA ...

EndoFree Plasmid Kits

... For purification of endotoxin-free ultrapure plasmid or cosmid DNA Features and benefits Less than 0.1 EU/g plasmid DNA Yields of up to 10 mg plasmid DNA ...

HiSpeed Plasmid Kits

... For ultrafast purification of up to 750 g plasmid or cosmid DNA Features and benefits Less than 45 min (Midi) or 60 min (Maxi) from harvested culture to ...

Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications and Agarose Gels

... 1.5- to 3-ml liquid cultures, purified plasmid DNA can be obtained in an average of 2.5 minutes per miniprep. Plasmid and cosmid DNAs isolated with the StrataPrep plasmid miniprep kit are easily digested with restriction enzymes ( figure 2 ). ...

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Cosmid in Biological Products

pWEB::TNC Deletion Cosmid Transposition Kit from EPICENTRE Biotechnologies

Description:... The pWEB::TNC™ Deletion cosmid Cloning Kit enables you to prepare, in about two days, a complete and unbiased primary cosmid library of genomic DNA for subsequent use in making nested deletion sublibraries. High efficiency cosmid library construction is accomplished using easy ...
Company:EPICENTRE Biotechnologies

pWEB Cosmid Cloning Kit from EPICENTRE Biotechnologies

Description: ...
Company:EPICENTRE Biotechnologies
Cosmid in Biological Dictionary

Cosmid

... A cosmid , first described by Collins and Hohn in 1978, is ... can be used to build genomic libraries. cosmid n. Genetics. A hybrid vector that has been ... large ... · plasmid · lambda phage · cosmid · P1 phage · fosmid · BAC ... Information ...

Overlapping clones

... because one of its end se ... To generate contigs, overlapping cosmid clones were identified primarily by ... If two cosmid clones are overlapping , the common bands produced on complete ... ....

Genomic library

... fragments that have ... ... received amplified aliquots of two genomic libraries for Aspergillus fumigatus. ... pWE15 and pLORIST2 genomic cosmid libraries of A. nidulans ... Second, we digest the genomic DNA of an organism that can metabolize lactose ... this strategy to make a ...

Contig map

... Wheat Contig Maps by Searching WheatDB ... Contig Assembly: FPC v6.0 is used to assembly contigs. ... A physical map is the ordering of cosmid clones by their position along ... A. nidulans mapping project, a contig map of P. carinii should ... of contig maps . ... Contig ...

Contig

... 3 dice as the starter square. ... on the Contig board. Put an X on this number. ... Contig Game. tkawas@mathwire.com ... The more detailed cosmid contig map depicts the order of overlapping DNA frag ... between fragments than contig maps (see below) ... Contig construction can be ... ...

Chromosome jumping

... applied the technique of chromosome jumping to this region. ... of a general human chromosome jumping library, with application to ... cosmid walking and chromosome jumping in the region of PKD1 reveal a locus ... A third CpG island was detected and cloned by directional chromosome ...

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Other Contents
(Date:7/10/2014)... Up to 70% of Parkinson,s disease (PD) patients ... of life. Some patients have disturbed sleep/wake patterns ... while other patients may be subject to sudden ... PD patients may exhibit REM-sleep behavior disorder (RBD), ... even before motor symptoms appear. A review in ...
(Date:7/10/2014)... the human brain operates much the same whether active ... a better understanding of schizophrenia, bipolar disorder and other ... million Americans. , In newly published research in the ... at the Center for Molecular and Behavioral Neuroscience, determined ... rest is basically the same as that of a ...
(Date:7/10/2014)... Manchester scientists have shown that a new drug could prove ... aggressive form of lung cancer. , Scientists from the Cancer ... and part of the Manchester Cancer Research Centre, teamed up ... in 2010, to test a drug known as AZD3965 ... published in the journal Clinical Cancer Research , also ...
(Date:7/9/2014)... July 10, 2014 For the first time, researchers ... Aboriginal population in Canada uses health care. A new ... to clearly demonstrate the unique challenges faced by urban ... St. Michael,s Hospital. , The findings, published today ... urban First Nations individuals and the general population. , ...
(Date:7/9/2014)... hidden ways that cells are regulated, scientists at Rockefeller ... cells more likely to metastasize. , What,s more, the ... blocking two other proteins that are normally linked to ... could have unexpected ties. , The study, which ... , points to the possibility of new cancer therapies ...
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(Date:7/10/2014)... Cancer Research Centre (CNIO) Macromolecular Crystallography Group has ... called BuD to DNA in order to redirect ... researcher who led the study, says the discovery: ... instructions contained in the genome to treat genetic ... is published in the journal Acta Crystallographica, ...
(Date:7/10/2014)... natural-color satellite image was collected by the Moderate Resolution ... 10, 2014. Each hot spot, which appears as a ... on the MODIS instrument recognized temperatures higher than background. ... image, such hot spots are diagnostic for fire. ... little danger coming directly from the fires, although the ...
(Date:7/10/2014)... (July 10, 2014) Consuming a whole fresh avocado ... significantly enhanced provitamin A carotenoid (alpha- and beta-carotene) absorption ... of vitamin A, according to new research (1) ... , Vitamin A is involved in reproductive ... function, and vision; and has antioxidant properties. Provitamin A ...
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