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Anneal in Biological News

'Virus chip' detects new virus in prostate tumors

... Researchers then wash fluorescently tagged DNA from a sample of interest over the slide, and wherever the two sets of nucleic acid match up, they anneal to each other. The slide is then rinsed and visualized with a scanning laser microscope. The dots that have found a match glow with fluorescent ....
Anneal in Biological Technology

Use of the DCode System to Detect the Food-Borne Bacterial Pathogen Listeria monocytogenes

... gene of L. monocytogenes, Listeria innocua, and Listeria seeligeri by the polymerase chain reaction (PCR)* using primers that anneal to conserved sequences, yielded multiple copies of gene fragments that, when evaluated by density gradient gel electrophoresis ...

Rapid, Reproducible Real-Time Quantitative RT-PCR Using the iCycler iQ Real-Time PCR Detection System and iQ Supermix, Rev A

... II reverse transcriptase (Invitrogen) according to the manufacturers instructions. Gene-specific primers (GSPs) were designed to anneal near the 3' end of two mRNA transcripts and to generate PCR products 75300 base pairs long. Three GSP pairs amplify different ...

RNA Amplification for Array Analysis

... the typical probe orientation for microarrays? Oligonucleotides are single-stranded and therefore only anneal to their complement. Probes for microarrays are either generated from an RT reaction (cDNA) or from linear ...

Thermal Cycling Profile for Standard PCR

... heating of the PCR mixture for 2 minutes at 9495C is enough to completely denature complex genomic DNA so that the primers can anneal to the template as the reaction mix is cooled. If the template DNA is only partially denatured, it will tend to snap-back very ...

Primer design

... the required primers (see figure below). Make primers that anneal to sequences in exons on both sides of an intron (Panel 1). With these primers, any product amplified from genomic DNA will be ...

QIAGEN Multiplex PCR Handbook

... of the primer mix. 9. Amplification of pseudogene sequences Primers may anneal to pseudogene sequences and additional PCR products may be amplified. Review primer design to avoid detection of ...

Using the TripleMaster PCR System for Robust Amplification of GC-Rich DNA Templates

... renaturation of the template and the formation of secondary structures within each strand before the primers have a chance to anneal to their specific template sites. These properties of GC-rich DNA sequences lead to inefficient primer-template annealing and ...

Expression and Purification of Recombinant Proteins That Have Native Amino Acid Sequence

... that are complementary to the single-stranded tails of the pCAL-n-EK vector. The vector and insert DNA are combined, allowed to anneal at room temperature and transformed into highly competent bacterial host cells. The resultant colonies can then be screened for ...

High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli

... is purified, then treated with Pfu DNA polymerase in the presence of dATP. The vector and insert DNA are combined, allowed to anneal at room temperature, and transformed into highly competent bacterial host cells. The resultant colonies can then be screened ...

Map and Link Human Genetic Disorders with SSLP Analysis

... location. 1 Often these markers are (CA)n repeat polymorphisms. 1 These polymorphisms can easily be genotyped by PCR with primers that anneal to single-copy DNA, which flanks the repetitive element. Genotyping, using microsatellite markers, at specific locations throughout the ...

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Anneal in Biological Products

CpG WIZ® DAP-KINASE AMPLIFICATION KIT from CHEMICON

Description:... DNA. The primer sets in the kit are engineered to anneal to the DNA, based upon the sequence differences. U Primer Set will anneal to unmethylated DNA that has undergone a chemical modification. M Primer Set will anneal to methylated DNA that has undergone a chemical ...
Company:CHEMICON

BioPrime Plus Array CGH Indirect Genomic Labeling Module from Invitrogen

Description:... and efficient incorporation of Alexa Fluor AHA fluorescent nucleotides. In the BioPrime Plus Array CGH Genomic Labeling Systems, random octamers anneal to template DNA, providing priming sites for the exo- Klenow enzyme. Alexa Fluor AHA modified nucleotides (direct labeling) or amino-allyl modified ...
Company:Invitrogen

BioPrime Array CGH Genomic Labeling System from Invitrogen

Description:... and efficient incorporation of Alexa Fluor AHA fluorescent nucleotides. In the BioPrime Plus Array CGH Genomic Labeling Systems, random octamers anneal to template DNA, providing priming sites for the exo- Klenow enzyme. Alexa Fluor AHA modified nucleotides (direct labeling) or amino-allyl modified ...
Company:Invitrogen

Two-Color RNA Spike-in Kit from Agilent Technologies

Description:... The Agilent Two-Color RNA Spike-In Kit contains in vitro synthesized, polyadenylated transcripts that specifically anneal only to complementary control probes on Agilents microarrays, with minimal self- or cross-hybridization. The spike-ins serve as a positive microarray ...
Company:Agilent Technologies

One-Color RNA Spike-in Kit from Agilent Technologies

Description:... The Agilent One-Color RNA Spike-In Kit contains in vitro synthesized, polyadenylated transcripts that specifically anneal only to complementary control probes on Agilents microarrays, with minimal self- or cross-hybridization. The spike-ins serve as a positive microarray ...
Company:Agilent Technologies

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Anneal in Biological Definition

Polymerase chain reaction

... end of the DNA fragment to be amplified. They anneal (adhere) to the DNA template at these starting ... as the temperature below which the primer will anneal to the DNA template and above which the primer ... of the primer. Primers that are too short would anneal at several positions on a long DNA template, ...

Primer

... end of the DNA fragment to be amplified. They anneal (adhere) to the DNA template at these starting ... as the temperature below which the primer will anneal to the DNA template and above which the primer ... of the primer. Primers that are too short would anneal at several positions on a long DNA template, ...

Genetic recombination

... the nicked strand to pull apart from its complementary strand and anneal to one strand of the double helix on the opposite chromatid. A second ... allows the unannealed strand in the second double helix to pull apart and anneal to the remaining strand in the first, forming a structure known as a ...
Anneal in Biological Dictionary

Anneal

... is a heat treatment wherein a ... A full anneal typically results in the second most ductile ... · Glass batch calculation · Glass forming ... anneal - Definition of anneal at Dictionary.com a free online dictionary with ....

Sticky ends

... ... You'll come to a sticky end . Cohesive. Benight. complimentary. molecule. DNA ... Name the bonds that are formed when sticky ends anneal and those that are formed when blunt ends anneal. This results in both ends having a single stranded area called sticky ends . ... this ...

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