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AGAROSE in Medical News

Scientists Build a Better Petri Dish

... say the biomedical engineers at Brown University in Providence, R.I., who invented the new dish. The dish is made from a sugary substance called agarose -- long used in laboratories -- that allows cells to self-assemble naturally and form "microtissues," the scientists explained in an article ...
AGAROSE in Medical Products

Agarose MP

Description:... agarose MP, a multipurpose agarose, has been developed ... DNA [pulsed-field gel electrophoresis (PFGE)]. agarose MP is a high gel strength agarose ( 1800 g/cm2, 1%). The high gel strength ...
Company:Roche Applied Science

UltraClean™ Forensic Agarose

Description:... UltraClean Forensic agarose is a premium agarose used for isolating DNA fragments larger than 1000bp. This agarose produces clear sharp bands, with no smearing and ...
Company:Mo Bio Laboratories, Inc.

UltraPure™ Agarose 1000

Description:... UltraPure™ agarose 1000 is specifically formulated for high ... <1000 bp. This standard melting temperature agarose can resolve DNA fragments with as little as 10 bp difference in size. UltraPure™ agarose 1000 gels exhibit a strong gel structure for ...
Company:Invitrogen

UltraPure™ Agarose

Description:... UltraPure™ agarose resolves DNA and RNA fragments from 100 bp to >30 kb. UltraPure™ agarose is a standard melting temperature multi-purpose agarose that is ideal for routine separation analysis. ...
Company:Invitrogen

NuSieve® 3:1 Agarose

Description:... NuSieve 3:1 agarose is a molecular biology grade, standard melting temperature agarose that yields strong gels for fine resolution of ... and blotting. Performance testing of NuSieve 3:1 agarose ensures fine resolution of DNA fragments up to ...
Company:Cambrex

SeaKem® GTG® Agarose

Description:... SeaKem GTG agarose is a standard gelling temperature, high gel strength agarose that resolves DNA fragments greater than 1,000 bp. This Genetic Technology Grade™ (GTG) agarose is specifically designed for preparative DNA ...
Company:Cambrex

Uniphor electrophoresis units

Description:... Preparative Protein and Peptide Separation: Non-denaturating PAGE gels and agarose gels IEF in agarose Ampholyte Gels (Z64,071-9& Z64,075-1) Molecular Biology: DNA/RNA fragment ...
Company:Sigma-Aldrich

UltraClean™ MS-8 Agarose

Description:... UltraClean MS-8 agarose is an agarose for molecular screening that improves resolution of small DNA fragments ...
Company:Mo Bio Laboratories, Inc.

Agarose 3:1 HRB™ - High Resolution Blend

Description:... agarose 3:1 HRB™ (High Resolution Blend), is a unique mixture of agarose formulated to provide high resolution of small ... analysis of DNA fragmanets less than 1,000 bp. agarose 3:1 HRB™ displays precise banding patterns ...
Company:MIDSCI

Agarose NA

Description:... agarose with low electroendosmosis (EEO) is recommended ... endonucleases, a special highly-purified agarose such as agarose NA should be ....
Company:GE Healthcare, formerly Amersham Biosciences

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AGAROSE in Biological News

DNA fingerprinting simplified

... KNOXVILLE, TN agarose gel electrophoresis" Most teenagers wouldnt have ... used tools in biochemistry and biotechnology, agarose gel electrophoresis is a common forensic ... molecules through a gelatinous substance known as agarose by using an electric field. Andrew, in ...
AGAROSE in Biological Technology

Eniva VIBE(R) Nutraceutical Tested at Linus Pauling Micronutrient Research Institute and Found to Possess DNA Protective Properties

... is widely used to assess DNA damage in cancer research, environmental toxicology and radiation biology. After mutagen exposure, cells are embedded in agarose gel on a microscope slide, lysed, electrophoresed, and then stained with fluorescent DNA binding dye. Damaged DNA migrates during the process, ...

Use of the DCode System to Detect the Food-Borne Bacterial Pathogen Listeria monocytogenes

... 9 Amplified products were analyzed by agarose gel electrophoresis as follows: 5 ml product ... Center, MI) and the mixture applied to a 1% agarose low melt preparative grade gel ... Hercules, CA) was added to the agarose solution at a concentration of 0.5 mg/ml before ...

Aurum Plasmid 96 Kit Tech Note, Rev A

... from the 4 OD culture were run on agarose gels to confirm purity and concentration of the ... processed. agarose Gel Electrophoresis When plasmid samples isolated from the 4 OD culture were run on an agarose gel, Aurum plasmid 96 samples were ...

Electrophoretic Karyotypes of Wine Strains of Saccharomyces cerevisiae

... volts, 1.8 liters of 0.5X TBE buffer, 65 ml of 1% agarose (Ultra Pure DNA Grade, Bio-Rad ... a cooling bath. Samples of cell-containing agarose beads, 35 l, were loaded into wells ... drops of molten 1% Low Melt Preparative Grade agarose (Bio- Rad Laboratories). Gels were ...

Advantages of ReadyAgarose Precast Gels Over 96-Well Handcast Gels for High-Throughput Analysis, Rev A

... Introduction Running PCR samples on agarose gels is a routine yet timeconsuming procedure. ... gels are available in 0.8%, 1%, and 3% agarose in TBE or TAE buffer, with or without ... adding 8.7 g of Bio-Rad Certified low range ultra agarose (the ...

PFGE Analysis of Chromosomal DNA From a Single Hair Root

... Preparation One percent low melt agarose (SeaPlaque, FMC) was prepared in 1x PBS, ... Prior to digestions, excess agarose was trimmed to within 2 mm of the ... system. The gel was 1.0% agarose (SeaPlaque) in 0.5x TBE buffer chilled ...

Quantitation of GAD67 Gene Expression in Prefrontal Cortex of Schizophrenia Patients Using the iCycler iQ Detection System and Molecular Beacons, Rev A

... and quality of total RNA was also analyzed by agarose gel electrophoresis. Reverse ... were analyzed on ethidium bromide-stained agarose gels. The primer pair that generated ... were analyzed on ethidium bromide-stained agarose gels. In contrast to their respective ...

Resolution of Linear DNA Fragments From 23 Kilobases to 6 Megabases Using Biphasic Linear Switch-Time Ramping

... using a Bio-Rad CHEF Mapper system), and/or agarose type and concentration 2 . Data is ... gel shown in Figure 1A was 0.5% Chromosomal Grade agarose made in 0.5x TBE (45 mM Tris borate, ... Lowering the concentration of agarose in gels can have the effect of ...

Detection of Genetically Modified Soybean in Processed Foods Using Real-Time Quantitative PCR with SYBR Green I Dye on the DNA Engine Opticon 2 System

... detect GMO material, using an agarose gel analysis of the reaction products.4, 5 ... by spectrophotometry and the quality verified by agarose gel electrophoresis on a 1% TBE gel. ... The reaction products were also examined on an agarose gel. The band pattern for all ...

DNA/RNA Blotting With the Trans-Blot SD Semi-Dry Electrophoretic Transfer Cell

... The Trans-Blot SD DNA/RNA agarose gel support frame, used with the Trans-Blot ... cell, allows blotting of DNA/RNA from agarose gels to nylon membrane in minutes, without any ... efficient transfers by eliminating crushing of agarose gels. DNA Transfers in 10 ...

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AGAROSE in Biological Products

UltraClean GelSpin (S) Kit Sample from Mo Bio Laboratories, Inc.

Description:... based spin filter membrane to isolate DNA from agarose gels. After electrophoresis, the desired DNA band is cut from an agarose gel and placed directly in the spin filter unit. ... membrane and washed once to remove traces of agarose and salt. The DNA is recovered from the spin ...
Company:Mo Bio Laboratories, Inc.

Chromosomal Grade Agarose from Bio-Rad

Description:... Chromosomal Grade agarose was developed specifically for pulsed field ... megabase pairs. This agarose has the highest gel strength of any electrophoresis agarose available, allowing ...
Company:Bio-Rad

GenElute Gel Extraction Kit from Sigma-Aldrich

Description:... of interest are extracted from slices of an agarose gel by solubilizing the gel. The Gel Solubilization Solution can dissolve an agarose gel slice from gels run in either TBE or TAE ... DNA fragments from standard or low-melting agarose gels. This kit can also be used to purify DNA ...
Company:Sigma-Aldrich

GenElute Agarose Spin Columns from Sigma-Aldrich

Description:... GenElute™ agarose Spin Columns are designed for the rapid purification of linear 100 bp to 10 kb DNA from agarose gels. The DNA band is excised from an agarose gel ... are a series of membranes and filters that hold agarose and impurities back while allowing DNA to ...
Company:Sigma-Aldrich

Ultra Agarose Spin Kit from ABgene

Description:... The Ultra agarose Spin Kit is an advanced system for isolating DNA from agarose gels using spin filters. The purified DNA can be ... is ready to use in 56 minutes Low melting point agarose not required Can be used for TAE and TBE gels ...
Company:ABgene

GenElute Minus EtBr Spin Column from Sigma-Aldrich

Description:... (EtBr)-free DNA (100 bp to 10 kb) from stained agarose gels or EtBr containing solutions. The DNA band is excised from an agarose gel and loaded into the spin column. Embedded ... are a series of membranes and filters that hold agarose and ethidium bromide back while allowing DNA to ...
Company:Sigma-Aldrich

beta-Agarase from USB Corp.

Description:... agarose. Beta-Agarase digests the agarose into oligo- and disaccharides. Tested User ... to digest 200 l of melted low-melting-point agarose to oligo- and disaccharides in 1 hr at 40C. ... recovery of DNA from low-melting point agarose gels. PROTOCOL FOR RECOVERING FRAGMENTS FROM ...
Company:USB Corp.

AgarACE Enzyme from Promega

Description:... quantitative recovery of intact DNA or RNA from agarose gels. The enzyme is sufficiently thermostable that low melting point (LMP) agarose melted at 6575C does not have to be equilibrated ... Simply place the tube containing the molten agarose at 4247C and add AgarACE Enzyme. With slight ...
Company:Promega

UltraPure Agarose and SYBR Safe DNA Gel Stain combo from Invitrogen

Description:... UltraPure agarose and SYBR Safe DNA Gel Stain combo offers these ... to yourself, the environment and your institution agarose resolves DNA and RNA fragments from 100 bp to >30 kb. UltraPure agarose is a standard melting temperature, ...
Company:Invitrogen

UltraPure Agarose and SYBR Safe DNA Gel Stain Combo from Invitrogen

Description:... UltraPure agarose and SYBR Safe DNA Gel Stain combo offers these ... to yourself, the environment and your institution agarose resolves DNA and RNA fragments from 100 bp to >30 kb. UltraPure agarose is a standard melting temperature, ...
Company:Invitrogen

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AGAROSE in Biological Definition

Genetic fingerprinting

... fragments which are separated into bands during agarose gel electrophoresis . Next, the bands of DNA are ... a technique called Southern blotting from the agarose gel to a nylon membrane. This is treated with a ... genetic samples with some imperfection in the agarose gel. However, in this case, the laboratory ...

Polymerase chain reaction

... 3 : PCR product compared with DNA ladder in agarose gel. DNA ladder (lane 1), the PCR product in low ... PCR product can be identified by its size using agarose gel electrophoresis . agarose gel electrophoresis is a procedure that consists ...

Gel electrophoresis

... (greater than a few hundred bases ), the preferred matrix is purified agarose . In both cases, the gel forms a solid but porous matrix that looks and ... : Gel electrophoresis of large DNA or RNA is usually done by agarose gel electrophoresis . See the " Chain termination method " page for an ...

Gel electrophoresis

... (greater than a few hundred bases ), the preferred matrix is purified agarose . In both cases, the gel forms a solid but porous matrix that looks and ... : Gel electrophoresis of large DNA or RNA is usually done by agarose gel electrophoresis . See the " Chain termination method " page for an ...

Molecular biology

... DNA, RNA, and proteins can all be separated using an electric field. In agarose gel electrophoresis , DNA and RNA can be separated based on size by running the DNA through an agarose gel. Proteins can be separated based on size using an SDS-PAGE gel. ...

Affinity chromatography

... and possibly antibodies against the GST tag as well. The protein can then be covalently coupled to a solid medium such as sepharose or agarose (these are commercially available). For thoroughness the GST protein and the GST-fusion protein can each be coupled separately. The serum is ...

Restriction fragment length polymorphism

... only cut where there are specific DNA sequences recognized by the enzymes. The restriction fragments are then separated according to length by agarose gel electrophoresis . The resulting gel may be enhanced by Southern blotting . Result The distance between the locations cut by restriction ...

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AGAROSE in Biological Dictionary

Gel electrophoresis

... DNA Gel electrophoresis is ... agarose gel electrophoresis is a method used in ... charged nucleic acid molecules through an agarose matrix ... gel electrophoresis n. ... is a widely used technique for ... agarose gel electrophoresis Digital image of 3 ...

Electrophoresis

... 4.3 Schematic diagram of electrophoresis ... agarose gel electrophoresis is an easy way to ... Learn the basic information you ... Topics for agarose Gel Electrophoresis . Get Help on your ... An ... buffer, usually Tris ... Virtual Lab: agarose Gel Electrophoresis of Restriction Fragments ......

Denaturing Gel

... apparatus from 2cm to 12cm. ... Denaturing agarose gel is prepared by melting ... How to run denaturing agarose gel faster? Length variable electrophoresis apparatus from 2cm to 12cm. ... Denaturing agarose gel is prepared by melting the ... ...

Agarose gels

... agarose gel electrophoresis is a method used in ... charged nucleic acid molecules through an agarose matrix ... Embitec manufactures & ... ... Follow these simple steps for running an agarose gel to separate and visualize different size ...

Agarose Gel

... agarose gel electrophoresis is a method used in ... charged nucleic acid molecules through an agarose matrix ... ... necessary for conducting agarose gel electrophoresis are relatively simple ......

Ethidium Bromide

... tag in molecular biology laboratories for techniques such as agarose gel electrophoresis. When exposed to ultraviolet light, it will fluoresce ... ... 3 Visualisation: Ethidium Bromide (EtBr) and dyes. 4 Percent agarose and resolution limits ... Latex gloves do not protect well from ...

Streptavidin

... for biotin and is used ... structure of a streptavidin monomer is composed of ... Streptavidin biotin-binding protein immobilized to agarose beads, UltraLink ... Streptavidin generally has less nonspecific binding than avidin (from chicken ... Streptavidin products that produce ...

Southern blotting

... blot is a method routinely used in molecular biology for detection of a specific DNA sequence in DNA samples. Southern blotting combines agarose gel electrophoresis for size separation of DNA with methods to transfer the size-separated DNA to a ... The Southern blot is used ...

Southern blot

... blot is a method routinely used in molecular biology for detection of a specific DNA sequence in DNA samples. Southern blotting combines agarose gel electrophoresis for size separation of DNA with methods to transfer the size-separated DNA to a ... Professor Sir Edwin Mellor ...

RFLP

... with sickle cell anemia. ... By performing RFLP analysis we obtain a diversity estimate for a particular ... Prepare a 3% NuSieve (Cambrex) agarose gel for RFLP . ... Perform PCR with Beckman D4-labeled (5'-end) forward 18S rDNA primer and ... Custom D4-labeled primers are ordered from ...

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