vivo

Gel Offers A More Targeted Approach

...y. However they say the next step is to conduct in vivo studies of the hydrogel system so it can be modified to produce oral pills....

Detecting DNA Damage With In-Vivo Alkaline Comet Assay

...which has resulted in increasing demand for the in vivo Comet assay. In response to this demand, RCC Cytot... assay experiments were run in parallel with an in vivo UDS test on the same hepatocyte suspensions. The results have shown that the in vivo Comet assay is ...

Gene-Nanoparticle Gene Transfer Is Better Than Viral Vector Transfer System

...at a non-viral vector has demonstrated efficacy in vivo at levels comparable to a viral vector," Bharali said. Using a new optical fiber in vivo imaging technique (CellviZio developed by Mauna Kea Technologies of Paris), the UB researchers were ...

New Discovery May Help Treat Infertility

...e combination of the two different methods, the in vivo and in vitro studies, complement each other nicely and provide more precise answers to the role that the estrogen receptor beta plays in ovulation say researchers. Thus by making couples understand the reasons for their infertility, researchers say...

Its Possible To Grow Bone To Replace The Damaged Bone

...mpts to stimulate bone growth within the body ? in vivo ? have had limited success but have proven to be extremely complex, expensive and unreliable. Shastri and his colleagues took a new approach that has proven to be surprisingly simple. They decided to take advantage of the body?s natural wound-heal...

Historic trial for tuberculosis launched together by leading drug companies

...B infection faster than the existing treatment. In vivo preclinical studies have already shown that the drug significantly reduces treatment time by two months. If the trials are successful, the partnership aims to register moxifloxacin for a TB indication and is committed to making it affordable and acce...

Novel Technique For Targeting Small-Cell Lung Cancer

...ne of the more promising conjugates were tested in vivo by fiber-optic spectrofluorimetry and quantitative spectral imaging, on an H69 human SCLC tumor mouse xenograft model. The lead compound showed exceptional tumor/normal tissue ratios, ranging from 9 to 90, and had potential for targeting SCLC overe...

Passive Cigarette Smoking Increases Isoprostane Formation

...ar damage. Isoprostanes, a reliable marker of in vivo oxidation injury, have been shown to increase in a...5) document a significant temporary increase in in vivo oxidation injury due to passive smoke favouring development and/or progression of vascular disease. ...

Exercise Can Strenghten Knee Cartilage

... in moderate, supervised exercise. This in vivo cartilage monitoring study in patients at risk of knee OA who begin exercising indicates that adult human articular cartilage has a potential to adapt to loading change. Moderate exercise may thus be a good treatment not only to improve joint...

Breakthrough In New Vaccine For Malaria

...ility and complexity. The scientists also did in vivo experiments in mice, infecting them with the parasite and then injecting a small amount of the vaccine-produced antibodies. The antibodies significantly cut the number of parasites in the blood of these animals and in some cases wiped them out entir...

New Procedure Reveals the Secrets of the Brain

...ng the utility of their technique in uncovering in vivo functional connectivity maps. Use of the microstimulation/MRI technique in conscious, alert primates holds great promise for determining the causal relationships between activation patterns across distributed neuronal circuits and specific behaviors...

Breast Tumor Metastasis Stimulated by Post Pregnancy Circumstances

... virgin matrix. The group thereafter conducted in vivo studies to confirm the hypothesis that post-lactation involution matrix promotes tumor cell migration, called metastasis.. The alterations in the extra cellular matrix that take place post-pregnancy involution may actually bring about metastasis o...

Discovery Of New Molecule Could Solve Cancer Spread Mystery

...ogression of lethal malignant glioma cells both in vivo as well as in vitro. This study reinforces the concept that successful small molecule kinase inhibitors must display a broad reactivity to effectively attenuate the complex signaling pathways involved in malignant transformation and to thwart to th...

Advance in Program for Development of Non-Intravenous Therapy for Hemophilia

...eatment of hemophilia. Extensive in vitro and in vivo analysis has identified several synthetic lead molecules with promising pharmacological properties. "Using our proprietary technology platform, we continue to make important progress in our collaboration with Baxter, the leader in hemophilia therap...

Broad Spectrum Antibiotic Available for Antibiotic Resistant Bacteria

...by comprehensive global in vitro studies and an in vivo clinical trials programme. The most common adverse events reported in clinical trials with tigecycline were nausea and vomiting. These occurred early in the treatment and were generally mild or moderate in severity. Source : Eurekaert...

Experiments In Outer Space To Determine single Cell Survival

... latency period. TB bacteria can remain dormant in vivo for 30-50 years and then re-activate to cause disease. Presently the state of the dormant bacterium is not understood. Timmins and Brinker are discussing further experiments to validate the model. vvFinally, building the cells into a coating with a...

Muscular Dystrophy Reversed In Lab Mice

...scle function. "The results represent the first in vivo proof of principle for a therapeutic strategy for treatment of myotonic dystrophy by ablating or silencing expression of the toxic RNA molecules,' the authors said. In MD there is a faulty DNA, which is responsible for almost all of the abnormaliti...

Nanotechnology – to detect and treat ovarian cance

...nder development to follow the tissue stiffness in vivo to diagnose cancer. A second area of research involving nanotechnology uses viral particles as templates to fabricate uniform, nanometer imaging probes and drug carriers. The research team is extracting the DNA from viral particles and replacing it...

First Study to Show How Immune Cells 'Speak' to Each Other in Vivo

...l significance of mature immunological synapses in vivo during antiviral immune responses. The findings wi...ow immunological synapses 'speak' to each other in vivo has not been observed prior to this research. Although formal proof of this awaits the development o...

Molecule responsible for age associated macular degeneration identified

...f CEPs found in the eye. The researchers did in vivo animal studies with membranes from chicken eggs and rat eyes and found that CEPs attached to proteins induce angiogenesis. They also found that the protein part of CEP-protein adducts is not important for producing the growth of the blood vessels. Ra...

Hybrid Nanoparticles for Multimodal Medical Imaging

...e nanoparticles as contrast agents for improved in vivo imaging. The Oishei grant, "Developing New Advances in Medical Imaging through Nanotechnology," will be used in part to recruit and support a research professor to provide expertise in ultrasound imaging, as well as postdoctoral fellows and graduat...

Turmeric prevents rheumatoid arthritis: US study

...arthritis. This study also provides the first in vivo documentation of a mechanism of action - how curcumin-containing extracts protect against arthritis. The researchers found that the curcuminoid extract inhibits a transcription factor called NF-KB from being activated in the joint. A transcription ...

Blame Myelin for Many Neuropsychiatric Disorders

...is, his hypotheses can easily be tested through in vivo imaging of the brain to study the breakdown and growth of myelin. That will make it possible to directly test in humans the practical utility of the myelin-centered model of the human brain. Ultimately, it could foster the development of novel trea...

A New Research Demonstrates HIV-1 In Resident Immune Cells Of Human Testis

... which retained the same tissue architecture as in vivo tissue and continued to secrete testosterone, was able to support infection by HIV-1. Virus produced from the testis culture was fully active as collected virus was able to infect permissive cells in culture. The finding that HIV-1 can replicate w...

Echinacea: Cold-Season Cure or Risk?

...een no published information about their effect in vivo – in the human gastrointestinal tract. Research conducted by Jerald C. Foote, assistant professor of food and human nutrition, and graduate student Laura Hill yielded some surprising results and pointed to the need for further research. The researc...

First Potential Treatment for Dengue

...ic metalloproteinases inhibitors. A series of in vivo experiments following the same principle confirmed these hypotheses. A mouse model with blood circulatory system colored blue was injected with supernatant containing these enzymes, on their own or in the presence of their inhibitor. This procedure n...

Glucose Sensor That Controls Glucose Metabolism Identified

...dicating that LXR functions as a glucose sensor in vivo that responds to increasing liver glucose uptake. The ability of the LXRs to respond to glucose and its derivatives was very specific: no effect was seen in other nuclear receptors tested. The current study focused primarily on the role of glucose ...

Researchers Investigate Molecular Basis of Memory

... Sevilla, Spain, who performed the behavior and in vivo recordings. "To assess if the molecular mechanisms that generate LTP also underpin memory formation you need to study a living animal while it is learning." Minichiello and her team combined molecular, electrophysiological and behavioural methods ...

Europeans Developing First-Ever Dengue Fever Vaccine

... metalloproteinase were again supported through in vivo testing in mice. The results uncovered by the European-led international team shed new light on Dengue’s pathological strategy. Researchers now suspect that they have not only come across a defence strategy for Dengue, but for a whole range of haem...

New Hope for Regenerative Medicine

... had some capacity to form tissues in vitro and in vivo but it was questionable if these embryonic stem cells could generate transplantable material that would proliferate and replace tissues in an adult." The researchers took a two-step approach: First they injected uniparental ES cells into wild-type...

New Imaging Technique Tracks Traffic Patterns of White Blood Cells

...r intravital observations of leukocyte behavior in vivo by allowing the near-simultaneous observation of c...es will greatly aid investigations to elucidate in vivo imflammatory cell behavior, and provide a powerful tool for the development of therapeutics for thes...

Common Ingredient in Big Macs and Sodas Can Stabilize Gold Nanoparticles for Medical Use

... biocompatible properties, they can be used for in vivo (inside the bod)) molecular imaging and therapeuti...r therapy. Gold nanoparticles could function as in vivo sensors, photoactive agents for optical imaging, drug carriers, contrast enhancers in computer tomog...

Philadelphia Researchers Found A Novel Way To Fight Several Kind of Cancers

... The technique is the first in the new field of in vivo molecular-genetic imaging that doesn't require transfecting tumors with a "reporter" gene, the scientists say. "The beauty of this is that you don't have to introduce any reporter genes into the tumor because they are already there," says radiolog...

Science Builds Behind Pomegranate Extracts for Health

...kinetic parameters, absorption, metabolism, and ex vivo biological activities of a whole-fruit extract of pomegranate standardized to punicalagins in healthy human volunteers." Writing in the Journal of Agricultural and Food Chemistry, the researchers report that blood levels of the metabolites of punic...

No Carrier Necessary: This Drug Delivers Itself

...rystals that they continue to pursue. Further in vivo studies with HPPH nanocrystals are being conducted by scientists at UB and RPCI, including Pandey and Allan R. Oseroff, M.D., Ph.D., chair of the department of dermatology at RPCI and in UB's School of Medicine and Biomedical Sciences. The UB/RPCI ...

United States Department of Defense Selects Ichor Medical Systems to Develop Biodefense Vaccine

...ch company, is developing products based on the in vivo application of electroporation to enhance intracellular delivery of DNA drugs encoding therapeutic proteins or antigens for vaccines. Ichor's proprietary TriGrid(TM) Delivery System (TDS) is the first and only integrated and fully automated system fo...

A Novel Treatment for KSHV-infected Lymphomas

...that Nutlin-3a has striking anti-tumor activity in vivo in a mouse xenograft model for the PEL. Nutlin-3a treatment resulted in a marked regression of all tumors in the treated animals in two weeks. These results demonstrate that p53 reactivation via Nutlin-3a is an efficient treatment for KSHV-lymphomas ...

Micro-jets for Painless Needle-free Injections

...gher doses. Using insulin as a model drug for in vivo experiments with rats, the research team was able to demonstrate systemic delivery of macromolecules using their novel micro-jet injector. Although it takes a little longer for the drug treatment to reach the bloodstream using this delivery system ...

Deficiency in Vitamin D Increases the Risk of Cancer

... high-dose calcitriol treatment in in vitro and in vivo models. In the laboratory, there appears to be no tumor type that is uniquely sensitive to vitamin D treatment – leukemia, lymphoma, myeloma and solid tumor models are all sensitive, which speaks to the value of vitamin D as a potential therapeutic a...

Myelin Associated With Early Evolution of Huntington's Disease

...led in the very early stages using non-invasive in vivo neuroimaging markers of both myelin breakdown and levels of iron....

Scientist Observes real Time Generation of Neurones in Mammals

... model to study development of neural dendrites in vivo by using special microscopic imaging techniques, combined with virus gene technology. The dendrites are the string-like extensions of the neurone that spread out to reach other neurones, and serve as the points of communication between them. Th...

Astronauts can Now Feed on 'radiation-eating' Fungi

...the Einstein researchers performed a variety of in vivo tests using three genetically diverse fungi and four measures of cell growth. The studies consistently showed that ionizing radiation significantly enhances the growth of fungi that contain melanin. Both species grew significantly faster (as measu...

Nanomedicine Gives the Way for Nerve Cell Regeneration

...y. Using optic nerve and spinal cord tissues as in vivo models and dissociated retinal ganglion neurons as...y of using magnetic nanoparticles for enhancing in vivo axon regeneration, this work may have significant implications for the treatment of spinal cord inju...

Nanocomposite Labeled Cancer Cells Targeted and Destroyed by Using Lasers

...unctionality and payload than traditional small in vivo devices where the agent is conjugated directly to the surface. A laser can be used to kill cells indiscriminately, but it is really a blunt instrument. High powered lasers do so much damage that the tissue becomes opaque to further light. Yet, lowe...

DNA-damage Test Could Aid Drug Development

...esses, in vitro human models could be closer to in vivo human than in vivo mouse,' notes Shuga. 'That premise will be tested in coming years as new systems become available.'...

Antibody-based Therapies Effective at Controlling Malaria

...r P. falciparum antigens in mice and humans. An in vivo mouse model has significant advantage over the use of new world primates, the only other model for human malaria, which are labor-intensive and difficult to reproduce. These novel human reagents cured mice of an otherwise lethal malaria infection, ...

Insulin Therapy Shown to Treat Early Experimental Diabetic Retinopathy

...acodynamic properties were studied in vitro and in vivo using R28 cells in culture and SD rats respectively. Lead researcher Ravi S.J. Singh, MD, of the Pennsylvania State College of Medicine in Hershey, Penn., reports that low dose periocular delivery of insulin to the retina of diabetic rats does not ...

Study Reveals Little-known Cell Networks Vital to Circadian Rhythm

...aptive feature in most circumstances. SCN cells in vivo must synchronize not only to light-dark cycles but also to one another to coordinate circadian behavior. Lack of coupling may allow peripheral oscillators to anticipate and respond rapidly not only to the synchronizing cues emanating from the SCN but...

Antidepressents may Lower Bone Density in Older People

...theory is supported by findings of in vitro and in vivo laboratory investigations. Some data suggest that SSRIs may interfere with the function of osteoclasts and osteoblasts, cells responsible for the regular breaking down and rebuilding of bone in the body. Our findings suggest that, in this cohort, u...

Adherence of Nanoparticles to RBC- Very Effective Drug Delivery Method

...e of red blood cells dramatically increases the in vivo lifetime of the nanoparticles. Polymeric nanoparticles are excellent carriers for delivering drugs. They protect drugs from degradation until they reach their target and provide sustained release of drugs. Polymeric nanoparticles, however, suffer ...

Common Antidepressants Linked to Increased Bone Loss in Older Adults

...theory is supported by findings of in vitro and in vivo laboratory investigations," they write. "Our findings suggest that, in this cohort, use of SSRIs is associated with increased rates of hip bone loss. Further investigation of SSRI use and rates of change in bone mineral density in other populations...

Researchers Have Found the Role of Protein in Accelerating Breast Cancer Progression

...he major finding of our study demonstrates both in vivo and in vitro that inappropriate expression of PTN not only promotes breast cancer progression but that by itself PTN secretion from human breast cancer cells may be sufficient to shift that progression to a more aggressive form of breast cancer," he ...

New Understanding on Growth of Human Stem Cells

...ells (hdFs) in vitro despite removal from their in vivo microenvironment. These hdFs then provide a continuous source of supportive proteins, including insulin-like growth factor 2 (IGF-II), which they now show could be the protein to sustain hESCs. Researchers are interested in the relationship betwee...

EpiCept Accelerates Development of Phase I Clinical Study for Cancer

...nti-tumor activity in pre-clinical in vitro and in vivo studies. The molecule has been shown to induce tumor cell apoptosis and selectively inhibit growth of proliferating cell lines, including multi-drug resistant cell lines. Murine models of human tumor xenografts demonstrated EPC2407 inhibits growth of...