wax

Automated Fluorescent-Tag Cycle Sequencing

... models, eliminates the need for an oil or wax overlay on reactions. The Hot Top Assembly ... temperature cyclers eliminates theneed for oil or wax overlays on reactions during PCR cycle sequencing, whichmakes loading samples onto automated DNA seq...

Oil-Free PCR Using the Hot Top Assembly

...d gel loading was quicker andeasier without oil or wax overlays on reactions. Stratagenes line of RoboCy...s during cycling and eliminates the need foroil or wax overlays on PCR reactions. Because the Hot Top Assembly features anautomatic shut-off function and o...

Pfu DNA Polymerase as a Hot Start Alternative

...ally withheld by separation from the reaction by a wax barrier, 8 or the polymerase is reversibly inactiv... polymerase-specific antibody or magnesium wax beads), AmpliTaq Gold DNA polymerase or Pfu DNA polymerase. Similar comparisons were perform...

prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification

...nipulations (e.g., buffer additions and the use of wax barriers). RT-PCR Accuracy Is Influenced by DNA Polymerase Fidelity The frequency of mutations in RT-PCR products is thought to be influenced bythe replication fidelity of both the RT and the DNA polymerase, under theparticular reaction conditions ...

Greater Amplification Specificity with New Hot Start PCR Enzyme

... step 4,5 or withheld by a physical wax barrier until temperatures sufficient to melt wax are achieved. 6 More recently, reversibly inactivated DNA polymerases ...

FastStart Taq DNA Polymerase

...e of magnesium concentrations Eliminate wax barriers, beads, hot start antibodies, manual hot start, and the need to set up PCR reactions on ic...

Microfluidic analysis of multiplex PCR products for the genotyping of Helicobacter pylori

...emplateDNA derived from formalinfixed and paraffin wax embeddedtissue for the mPCR restricts thesize of the PCR fragments to amaximum of about 300 bp due tofragmentation and poor DNA qualityfrom such tissue. The primersets used in the mPCR for thedetection of the different H. pylorivirulence genes genera...

GenomiPhi amplification of FFPE tissue DNA for array CGH

...#60;P >6. Place on ice and remove any hardened wax with pipette tip. <P >7. Heat sample to 45 °C briefly. <P >8. Add 100 µl chloroform. <P >9. Agitate gently and centrifuge at 10,500 x g for 15 min. <P >10. Remove top phase (~180 µ...

Brazilian bio-industry should impress American investors

...ans. Brazilian biotechnology: Biofuels I could wax on even further about this trip, but interestingly during the trip Pope Benedict had just come to Brazil, as had Al Gore. President Bush had been there only a month or earlier, and struck an important U.S.-Brazil agreement on bio-fuels (bio-ethanol),...

Take off: Canadian biotech industry thrives

... provincial government. While I could continue to wax more eloquently on Canadian biotech and particularly the virtues of Toronto biotech, I would never get this column out to you. Needless to say, you need to visit this area, and yes, your Canadian hosts are very friendly. The Canadian government has ...