vesicles

High-content screening of GPCR activation with MetaXpress, AcuityXpress and the Transfluor assay system

... Background Correction system to distinguish pits, vesicles and nuclei from their local surroundings s...cell analysis of Transfluor data, pits and vesicles can be detected and distinguished simultaneously. The optional nuclear detection provides ac...

Visualizing siRNA in Mammalian Cells: Fluorescence Analysis of the RNAi Effect

... siRNA was being concentrated in lipid vesicles derived from the transfection carrier. To address this question, we repeated our transfections using an NBD-labeled carrier lipid (green) to transfect a Cy3 labeled siRNA (red). We examined the distribution of red ...

The use of CypHer5 for receptor internalization studies in both a range of GPCRs and a non-GPCR

...ion of cell surface receptors via acidic endosomal vesicles upon agonist stimulation (1, 2). <P >...y receptors that internalize into acidic endosomal vesicles upon ligand binding and activation. Dose response data were obtained for a range of different GPCR s...

The use of CypHer5E and the IN Cell Analyzer 1000 for live-cell receptor internalization studies

...face receptors internalizing into acidic endosomal vesicles upon agonist stimulation (4,5).<P >Using N...f receptors that internalize into acidic endosomal vesicles upon ligand binding and activation. Using the IN Cell Analyzer 1000, a high-throughput lamp based su...

Screening for β2-adrenergic receptor agonists using the pH-sensitive dye,CypHer5, and the IN Cell Analyzer 3000

...gic receptor internalization into acidic endosomal vesicles uses a red-excited pH-sensitive cyanine dye, CypHe...eta; 2-adrenergic receptors into acidic endosomal vesicles and would appear to be false positives. <P > Conclusion Agonist ac...

Gq-coupled receptor assays using FLEXstation: M1 muscarinic receptor

...mobilization of calcium from intracellular storage vesicles into the cytoplasm of the cell. Improper G q -coupled receptor function is implicated in cancer, neurodegenerative and cardiovascular diseases. Therefore, it is important to compare receptor function in normal and disease states. The cell line used ...