Untranslated in Biological Technology

Untranslated in Biological Technology

... for translation is obvious, but without knowledge of the 5' untranslated sequence, it is difficult to be certain that 'an' AUG in the cDNA sequence is 'the' AUG start site. The upstream, untranslated region will typically contain stop codons in-frame with the ...

Getting Started with MicroRNA Research

... represses protein translation by binding to sequences in the 3' untranslated region of specific mRNAs. The exact mechanism of translation ... miRNA activity levels. The cloning site in the 3' untranslated region (3' UTR) of the luciferase gene allows ...

The GeneZoo or the GeneJungle?

... splice variants, which occur less frequently in the 3 untranslated regions of transcripts (Table 1). Utilizing the 3 sequences as ... cluster sequences from different gene family members, since the untranslated regions tend not to align. In contrast, sequencing artifacts are ...

FlexiPlate siRNA

... shown that off-target effects are frequently caused by matches of the seed region of the siRNA antisense strand with the 3' untranslated region of mRNA (2). siRNA designs are analyzed for 3' UTR/seed region complementarity and siRNAs with the least potential to cause ...

The let-7 microRNA Family Regulates RAS: Implications for Development and Oncogenesis

... identified genes, let-60/RAS stood out because it has eight LCSs and ten nonconforming sites in the 3' untranslated region (3'UTR), as well as three LCSs in the coding sequence. The role of let-60/RAS in C. elegans ...

MicroRNA Profiling by Array Analysis Reveals Critical BioMarkers

... least two genes, lin-14 and lin-28 [13]. The activity of lin-4 depends on the partial homology of the miRNA to specific regions of the 3' untranslated regions (UTRs) of lin-14 and lin-28 mRNAs [5, 14]. Additional miRNAs have been functionally characterized (Figure 2). The range ...

miRNA Expression in White Blood Cells

... are endogenous, 21-24 nt RNAs that mediate post-transcriptional gene regulation by pairing with the 3' untranslated region of messenger RNAs and acting as translational repressors. The study of miRNA is an emerging and ...

Detecting miRNA & siRNA

... regulation. Some of these miRNAs (e.g. lin-4 and let-7 ) inhibit protein synthesis by binding to the 3' untranslated region of target mRNAs. Others bind to perfectly complementary mRNA sequences to destroy target transcripts ...

... specific miRNAs might be participating in cancer through the mis-regulation of oncogenes, we scanned the 3' untranslated regions (UTRs) of 150 well-known oncogenes for sequences with significant homology to the miRNAs identified ...

Human Universal cDNA Library Array I

... to hybridize to the beginning of the 5 translated region (ATG included) while the other corresponded to the end of the 3 untranslated region ( Figure 5 and Figure 6 ). The percentage of full-length cDNAs was determined by comparing the two results. ...

Detect Single Nucleotide Sequence Changes with Mx4000 Molecular Beacon Allelic Discrimination Kits

... ligand of CXCR4, the major co-receptor for T-tropic HIV-1 G to A substitution in the 3 untranslated region (SDF-1-3 A) 9 Caucasians (~20%) 9 Hispanics (~16%) 9 African ...