Translocation in Biological Technology

Biological electron transfer captured in real time

... The role of the respiratory chain is to couple electron transfer to the translocation of positively charged protons across the membrane, so that the ... ubiquinol. This reaction, as catalysed by Complex I, is linked to proton translocation across the membrane and thus leads to "charging the battery". At a later ...

Tigris Pharmaceuticals Presents Results of Breast Cancer Research at AACR Annual Meeting

... ovarian, lung and renal lines exhibiting particular sensitivity. The mechanism of action of AF is believed to be that treatment with AF results in translocation of the arylhydrocarbon receptor (AhR; a transcriptional regulator of CYP1A1) from the cytoplasm to the nucleus, and its subsequent interaction with ...

Medivation Announces New Positive Efficacy Data From Phase 1-2 Trial of MDV3100 in Castration-Resistant Prostate Cancer Patients

... prostate cancer. MDV3100 is a novel small-molecule androgen receptor antagonist that inhibits androgen receptor function by blocking nuclear translocation of the androgen receptor and DNA binding. About Medivation Medivation, Inc. is a biopharmaceutical company focused on the rapid ...

Japanese Cancer Association and Debiopharm Honour Japanese Research

... EML4-ALK (echinoderm microtubule associated protein like 4 - anaplastic lymphoma kinase) is a fusion protein resulting from a chromosomal translocation present in a relatively large percentage of NSCLCs (non-small cell lung cancers), a disease in which cancer cells form in the tissues of the lung. ...

Medivation Announces Presentation of 24-Week Clinical Data of MDV3100 in Castration-Resistant Prostate Cancer Patients

... prostate cancer. MDV3100 is a novel small- molecule androgen receptor antagonist that inhibits androgen receptor function by blocking nuclear translocation of the androgen receptor and DNA binding. About Medivation Medivation, Inc. is a biopharmaceutical company focused on the rapid development of ...

Performance of Ad-A-Gene EGFP-MAPKAP-k2: an adenoviral vector gene delivery system

... of an EGFP reporter assay for assessing the translocation of mitogen-activated protein kinase-activated ... of MAPKAP-k2 by p38 MAPK , and subsequent translocation of phosphorylated MAPKAP-k2 from the cell nucleus to the cytoplasm. translocation is thought to occur via phosphorylation-mediated ...

Performance of Ad-A-Gene EGFP-Glucocorticoid Receptor (EGFP-GCCR): an adenoviral vector gene delivery system

... application note presents data from an EGFP translocation assay focusing on the glucocorticoid receptor ... to imaging. Cells should be fixed at the peak translocation time point. Fixing of cells reduces the handling ... time. Dexamethasone-induced EGFP-GCCR translocation Figure 3 shows images, acquired on the IN ...

Using Validated siRNAs in Functional Genomic Assays

... pathway. Upon activation, NF-kappaB undergoes a translocation from its normal localization ... quantitate such nuclear translocation events. In the experiment ... of NF-kappaB nuclear translocation was used as a functional HCS ...

Screening for potential beta 2-adrenergic receptor antagonists using CypHer5E and IN Cell Analyzer 1000

... at acidic pH. This characteristic makes it ideal for monitoring the translocation of cell surface receptors into the endosomal pathway. Such translocation is a key element of receptor trafficking and activation, particularly in ...

Is Z' factor the best assessment for the quality of cellular assays delivering higher content?

... data from a variety of such assays we have developed, such as GFP translocation assays, including simple Z factor analysis and propose multiple ... We have developed a number of live-cell translocation assays that are compatible with high-throughput micro-imaging ...

Optimizing pfuturbo DNA Polymerase Amplification Reactions with Perfect Match PCR Enhancer

... secondary structure formation in the template strand, which impedes translocation of the DNA polymerase and limits amplification of longer targets. 5 ... structure formation in the template strand, which impedes translocation of the DNA polymerase. 5 Improved Specificity ...

ESR1 Gene Expression in Oncology and Metabolic Diseases Using the ASCENTATM System Target Validation and Identification of Novel Disease Indications

... Specific Receptor 1) is a member of the nuclear hormone receptor (NHR) family. NHRs bind to steroid hormones in the cytoplasm, causing their translocation to the nucleus and increased affinity for transcriptional elements governing the expression of certain genes (1). NHRs activate genes leading to cell ...

The use of CypHer5 for receptor internalization studies in both a range of GPCRs and a non-GPCR

... receptor is then measured as an increase in fluorescence in the cell. This offers an advantage over other techniques that rely solely on measuring a translocation in fluorescent signal. Many other commonly used techniques such as b-arrestin recruitment, Ca 2+ flux, and cAMP measurement are only suitable for ...

siRNA screening of the cell cycle with two dynamic GFP sensors (from Discovery Matters Issue 1, July 2005)

... the intervening period translocates from the cytoplasm to the nucleus.

The second cell line reports on G1 phase to S phase transition via translocation of an EGFP fusion protein incorporating the phosphorylation-dependent sub-cellular location domain (PSLD) of DNA helicase B (13). In this cell line a ...

RNAi as a Tool for Mammalian Gene Analysis: Applications of siRNAs

... ultimately showed that Akt2, and Akt1 to a lesser extent, has an essential role in insulin-stimulated GLUT4 translocation and glucose uptake in two different cell lines, whereas Akt1 and Akt2 contribute equally to ...

High-content screening of GPCR activation with MetaXpress, AcuityXpress and the Transfluor assay system

... The MetaXpress Transfluor module successfully identifies cells and measures pits and vesicles formed by beta-arrestin-GFP translocation to activated GPCRs in the Transfluor assay. Independent experiments with the beta-2-adrenergic receptor, both wild-type and ...

Cis-Reporting System Monitors Intracellular Calcium Mobilization

... reside in the cytoplasm. Stimuli that increase calcium mobilization result in the activation of NFAT proteins. The rapid dephosphorylation and translocation of NFAT proteins to the nucleus characterize activation; upon translocation, the dephosphorylated proteins show higher affinity for DNA. CsA or ...

Cis-Reporting System Monitors Intracellular Calcium Mobilization

... reside in the cytoplasm. Stimuli that increase calcium mobilization result in the activation of NFAT proteins. The rapid dephosphorylation and translocation of NFAT proteins to the nucleus characterize activation; upon translocation, the dephosphorylated proteins show higher affinity for DNA. CsA or ...