Transfection in Biological Technology

University of Lodz Invests in the Unique Cellaxess(R)HT System for Research on Multidrug Resistance Genes in Cancer

... the world's first system for high throughput transfection enabling reagent-free delivery of any genetic ... very significant for a technology enabling high transfection efficiency and viability in biologically relevant ... the Cellaxess(R)HT System enables high throughput transfection in genomic screening and the Dynaflow(R)HT System ...

Clontech Laboratories, Inc. Releases the Xfect(TM) and Xfect(TM) Stem Transfection Reagents

... 2009) release of the Xfect(TM) and Xfect(TM) Stem transfection reagents. These novel transfection reagents are based on biodegradable nanoparticles that permit superior transfection efficiency of plasmid DNA into mammalian cells. ...

MaxCyte Introduces the GT(TM) Flow Transfection System for Application with Autologous and Allogeneic Stem Cell Therapies

... today the introduction of its GT(TM) Flow transfection System at the 4th Annual Stem Cell Summit to be held in New York, NY. The MaxCyte GT(TM) Flow transfection System is a validated, scalable technology for ... a US FDA Master File, enables rapid and efficient transfection of any primary cell or cell line with >90% ...

The MaxCyte(R) STX(TM) Scalable Transfection System to be Presented at the Rapid Fire Innovation Session at LabAutomation2009

... presentation of the MaxCyte(R) STX(TM) Scalable transfection System at LabAutomation2009 in the Rapid Fire ... to the uniqueness of MaxCyte's large scale transfection technology. Using the MaxCyte STX, more than ten ... has designed the MaxCyte(R) STX(TM) Scalable transfection System expressly for rapid, scalable, and ...

MaxCyte Hosts Live Discussion with Industry Experts on the Challenges in Developing Cell-Based Screening Assays for Drug Discovery

... Dr. Reardon will review the complexities of cell transfection technologies and challenge the webinar ... MaxCyte STX technology enables the large scale transfection of primary cells, cell lines, and stem cells ... and operation of the MaxCyte STX Scalable transfection System. A recording of the webinar will be ...

Altogen Biosystems Launches New Nanoparticle-based In Vivo Transfection Reagent

... ) announced launch of novel Nanoparticle-based transfection reagent for use in laboratories and research facilities for animal testing. transfection is one of the major laboratory methods used to ... to the engineering, marketing and manufacture of transfection reagents and kits for intracellular ...

The MaxCyte STX Scalable Transfection System to be Launched at the SBS Symposium on Cell-Based Assays

... it will be launching the MaxCyte STX Scalable transfection System at the Society for Biomolecular Sciences ... "We believe that the MaxCyte STX Scalable transfection System addresses many of these challenges, ... MaxCyte STX technology enables the large scale transfection of primary cells, cell lines, and stem cells ...

MaxCyte Debuts the STX Scalable Transfection System at the A/C/T Assays & Cellular Targets Meeting

... at MaxCyte, "The MaxCyte STX Scalable transfection System can be used to transfect primary cells, ... and operation of the MaxCyte STX Scalable transfection System. About MaxCyte MaxCyte specializes in ... expertise, MaxCyte has designed the MaxCyte STX transfection System expressly for rapid, scalable, and ...

Sigma-Aldrich Introduces N-TER Nanoparticle siRNA Transfection System for Delivery of siRNA Into Difficult-to-Transfect Cell Types

... release of the N-TER(TM) Nanoparticle siRNA transfection System. N-TER allows researchers to ... reagents. "Traditional lipid-based siRNA transfection reagents exhibit a number of drawbacks, including ... these historic limitations and enables siRNA transfection to be performed in a wider variety of model ...

Senesco Signs a Supply Agreement for Polyplus-transfection's Delivery System

... and is reinvesting most of its revenues in research and development. transfection consists in introducing a gene or a small interfering RNA into cells. This ... The Strasbourg-based company is recognized as a leading innovator in the transfection market, with ISO 9001:2000 certification, exclusive licenses from the CNRS ...

Altogen Biosystems Launches Custom Biotechnology Research Services: Generation of Stable Cell Lines and RNAi Cell Transfection Services

... of stably-expressing cell lines, assay development, screening and transfection services. In particular, the company will draw upon their unique ... research services ( http://www.altogen.com/support.htm ) include: -- transfection services (transient and stable) -- Assay development and library ...

Celsense, Inc Receives NIH Grant to Translate Cell Imaging Technology for Clinical Use

... About Cell Sense Cell Sense (patent-pending) is a fluorocarbon tracer agent used to safely and efficiently label cells ex vivo without the use of transfection agents. Labeled cells are transplanted into the patient enabling researchers and clinicians to non-invasively track the administration and migration ...

Fate Therapeutics and Stemgent Launch Catalyst: A Unique Industry Program for First Access to the Most Advanced Induced Pluripotent Stem Cell Technology

... molecules for pluripotency, self-renewal, and differentiation, viral-delivered transcription factors, matrices, cell lines, cytokines, antibodies, transfection reagents, and more. This unique product mix is designed to serve researchers who study stem cell biology and regenerative medicine, and those who use ...

Selexis Launches Biosimilar Cell Line Development Program

... The Selexis' SURE Cell Line Development(SM) platform combines Selexis' Intellectual Property rights (e.g. novel genetic elements DNA sequences and transfection procedures) with its cell culture know-how for the rapid development of biosimilars products. Dr. Igor Fisch, President and CEO of Selexis, ...

Stemgent, CellDesign Sign Co-Exclusive Marketing Agreement on Stem Cell-Based Adipocyte Research Products

... and screened against stem cells, and includes small molecules for pluripotency, self-renewal, and differentiation, as well as cytokines, antibodies, transfection reagents, and more. Stemgent's latest innovations include viral vectors encoding transcription factors necessary for the generation of induced ...

Sigma-Aldrich Introduces MISSION(R) LentiPlex(TM) Pooled shRNA Libraries to Enable Rapid Whole Genome Screening in Primary Cells, Stem Cells or Tumor Cells

... difficult cell types such as primary, non-dividing, growth-arrested or terminally differentiated cells that are typically resistant to standard siRNA transfection or other viral delivery systems. In addition, Sigma-Aldrich's shRNA libraries include a U6 promoter for shRNA expression in a wide variety of cell ...

Gene Therapy Field Advances With Important Clinical Findings Reported in the New England Journal of Medicine

... the introduction of a normal adenosine deaminase gene into the patient's hematopoietic stem cells that have been removed from the bone marrow. After transfection of the cells with the gene, the cells are re-infused back into the patients after nonmyeloablative conditioning with busulfan. After a single ...

Cyntellect Appoints Former Millipore and BD Biosciences Executives to Head Up its Product Marketing Efforts

... combines expertise in high-speed cell imaging and laser-based manipulation to develop products that enable novel cell imaging, purification, and transfection capabilities to enhance the productivity of laboratory research, recombinant protein production, high-content cellular assays, functional genomics ...

Cyntellect Appoints Former BD Biosciences Executive Andre Lubarsky as Director of Sales - North America

... combines expertise in high-speed cell imaging and laser-based manipulation to develop products that enable novel cell imaging, purification, and transfection capabilities to enhance the productivity of laboratory research, recombinant protein production, high-content cellular assays, functional genomics ...

University of Florida and Cyntellect Collaborate to Unlock Mysteries of Cancer Stem Cells

... combines expertise in high-speed cellular analysis and laser-based manipulation to provide products that enable novel cell imaging, purification, and transfection capabilities to enhance the productivity of laboratory research, recombinant protein production, high-content cellular assays, functional genomics ...

Paper in Cell Cycle Reports Alfacell's ONCONASE(R) Targets siRNA

... abundant and ubiquitously expressed housekeeping gene) in human lung adenocarcinoma A549 cells by siRNA was effectively prevented by ONCONASE. While transfection of cells with GAPDH siRNA reduced expression of this protein by nearly 70 percent, the expression was restored in the cells exposed to ONCONASE ...

MaxCyte and Medinet to Present at the 10th International Symposium on Dendritic Cells in Kobe, Japan

... providing clinical/commercial cell modification technologies and unparalleled expertise to the global leaders in cell-based therapies. MaxCyte's cell transfection technology platform enables the discovery, development, manufacturing and delivery of innovative and important therapeutic products for a wide range ...

Bayer Broadens Its Manufacturing Basis for Biopharmaceuticals in Tobacco Plants

... will adapt its existing cGMP (cGMP = current Good Manufacturing Practice) compliant facility by installing an automated system for high throughput transfection of tobacco host plants. The agreement makes KBP the "preferred production partner" for the application of magnICON(R), an innovative technology for ...

Cyntellect Launches CellXpress(TM) System to Boost Productivity of Biopharmaceutical Process Development

... combines expertise in high-speed cell imaging and laser-based manipulation to develop products that enable novel cell imaging, purification, and transfection capabilities to enhance the productivity of laboratory research, recombinant protein production, high-content cellular assays, functional genomics ...

Cyntellect to Collaborate With the Burnham Institute for Medical Research

... combines expertise in high-speed cell imaging and laser-based manipulation to develop products that enable novel cell imaging, purification, and transfection capabilities to enhance the productivity of laboratory research, recombinant protein production, high-content cellular assays, functional genomics ...

Sigma-Aldrich Joins Phase 2 of The RNAi Consortium to Validate shRNA Libraries

... types such as primary, non-dividing, growth-arrested or terminally differentiated cells. Such cell lines are typically not amenable to standard transfection or genomic integration by other viral delivery systems, but they may be transduced efficiently with lentiviral particles. "Over the coming years, ...

Mirus Bio Awarded Grants & Contracts of Over $5 Million

... is being developed collaboratively with Transgene S.A. of Strasbourg, France. Finally, the company currently markets state-of-the-art DNA and siRNA transfection and labeling products to researchers worldwide. For more information go to: http://www.mirusbio.com ...

Americas Third Coast nurtures biotechnology

... in diagnostic laboratories or in field work. transfection Technology Mirus Technologies ( www.genetransfer.com ) offers nonviral gene transfection delivery products, which can be directly injected ... The companys product line includes 10 transfection reagents for specific cell lines, three ...

A Screen of shRNAs Targeting Tumor Suppressor Genes to Identify Factors Involved in Paclitaxel Sensitivity

... siRNA approach, we do not need to optimize transfection or reaction conditions for each new cell line ... pLKO.1-Puro vector allow for transient or stable transfection of the shRNA as well as production of lentiviral ... and non-dividing cells, overcoming low transfection and integration difficulties when using these ...

FLIPR Calcium 3 Assay Kit - An Optimal No-Wash Fluorescence Calcium Indicator Reagent for Non-Optimal Calcium Responses

... Accurate seeding density for cells prior to transfection is a major factor influencing the subsequent ... Lipofectamine Plus reagent and 24 hours following transfection were re-plated into poly-D-lysine coated 96-well ... That the results obtained for the 5-HT 2C transfection are sub-optimal is evidenced by data from other ...

siRNA screening of the cell cycle with two dynamic GFP sensors (from Discovery Matters Issue 1, July 2005)

... siRNAs were used as controls and to determine transfection efficiency. siRNAs were transfected into G2/M ... Results Cellular transfection efficiency was determined using a Cy5 labeled ... of Cy5 distribution and intensity indicated 90% transfection ....

Biolistic Transfection of Organotypic Brain Slices

... a device that could potentially allow transfection of such resistant cells (Klein et al., ... on optimizing the Biolistic parameters for transfection of dissociated cells and cultured ... us to apply Biolistic bombardment to transfection of both neuronal cell lines and brain slice ...

Commercial Plasmid Preparation Methods Yield DNA With Different Transfection Capabilities

... NY, USA Introduction transfection efficiency is dependent upon a number of factors, ... and purity. Liposome-mediated transfection of mammalian cells has proven to yield higher transfection efficiencies and is more reproducible ...

RNA Interference and Gene Silencing: History and Overview

... cells took longer to establish. transfection of long dsRNA molecules (>30 nt) into most ... Indeed, siRNAs introduced by transient transfection were found to effectively induce RNAi in ... can be induced in mammalian cells by the transfection of siRNAs, many more researchers are ...

YOUR DATA: Direct Real-time RT-PCR on Sympathetic Neuron Lysates Silencing of Focal Adhesion Kinases with Silencer Validated siRNAs

... was significantly reduced following transfection of cells with the PTK2B validated ... was significantly reduced following transfection of cells with the PTK2 validated siRNA but unchanged following transfection with the PTK2B validated siRNA. ...

YOUR DATA: Direct Real-time RT-PCR on Sympathetic Neuron Lysates Silencing of Focal Adhesion Kinases with Silencer Validated siRNAs

... was significantly reduced following transfection of cells with the PTK2B validated ... was significantly reduced following transfection of cells with the PTK2 validated siRNA but unchanged following transfection with the PTK2B validated siRNA. ...

Visualizing siRNA in Mammalian Cells: Fluorescence Analysis of the RNAi Effect

... correlate well with the release of siRNA from the transfection agent in the cytoplasm of transfected ... Lipid transfection Agent (Ambion, Inc.). 48 hr after transfection HeLa S3 cells were analyzed for c-myc ...

Streamline Your siRNA Transfections

... NEW siPORT NeoFX transfection Agent Promotes High ... Efficient--performs with high siRNA transfection efficiency, allowing use of ... of serum Streamlined transfection Protocol ...

siRNA Design: It's All in the Algorithm

... samples harvested 48 hr after siRNA transfection into HeLa cells. ... 2). Using Cenix-designed siRNAs in optimized transfection systems, Ambion researchers routinely ... samples harvested 48hr after siRNA transfection into HeLa cells. Percent of genes exhibiting ...