thiamine

A New C-Terminal GST Vector for Protein Production in S. pombe

...be is tightly repressed in the presence of thiamine (vitamin B 1 ) in the growth medium and is... thenresuspended into 10 ml of EMM media with 25 M thiamine (repressed) or withoutthiamine (induced) and grown at 30C for 18 to 20 hours. The cells werecollecte...

New Yeast Cloning System for Producing Proteins with Native Amino Acid Sequences

... such as tight transcriptional regulation by thiamine and high expression levels. The distinct a...esuspended in 10 ml of EMM media with 25 M thiamine (repressed) or without thiamine (induced) and incubated at 30C for 18 to 20 hours. Crude lys...

Production of Full-Length, Biologically Active MEK1WT and MEK1CA

...ase (when OD 600 reached0.5). In order to deplete thiamine in the media and induce protein expression,the cells were collected by centrifugation, washed twice with water, resuspendedin EMM media and grown at 30C for 18 to 20 hours. The cells were thencollected and broken by vortexing with glass beads in sorb...

Yeast Protein Production System Features High Yields and One-Step Purification

...be is tightly repressed in the presence of thiamine (vitamin B1) in the growth medium and is highly ac...owth medium(YES medium), which provides sufficient thiamine to repress the nmt1 promoter.Expression is induced by harvesting the cells and growing them in EMM b...

Kinetic determination of vitamin B1

...d the technique is applied to the determination of thiamine (vitamin B 1 ) in solution. Fluorescence intensity is monitored as thiamine converts to fluorescent thiochrome, and a relationship is established between the rate of increase o...