sucrose

Human Universal cDNA Library Array I

...is and storage while the other set was soaked in a sucrose solution and frozen at -80C to be used for further organization into microwell plates. Figure 2 Organization in Microwell Plates and DNA Preparation Figure 3 ...

Bifidobacterium animalis

... MRS broth with 0.05% cysteine.HCl and 0.5 M sucrose (final concentrations) Washing solution 0.5 M sucrose Electroporation solution 0.5 M sucrose, 1 mM ammonium citrate, ...

Listeria monocytogenes

...owth medium BHI, supplemented with 0.5 M sucrose Washing solution 1 mM HEPES (pH 7.0), 0.5 M sucrose Electroporation solution 1 mM HEPES (pH 7.0), 0.5 M sucrose ...

Propionibacterium freudenreichii

... Washing solution Ice-cold 0.5 M sucrose Electroporation solution Ice-cold 0.5 M sucrose buffered with 1 mM potassium acetate (pH 5.5) Outgrowth mediu...

Pseudomonas aeruginosa

... Washing solution 300 mM sucrose Electroporation solution 300 mM sucrose Outgrowth medium LB medium Cuvette ...

Bifidobacterium animalis

... MRS broth with 0.05% cysteine.HCl and 0.5 M sucrose (final concentrations) Washing solution 0.5 M sucrose Electroporation solution 0.5 M sucrose, 1 mM ammonium citrate, ...

Listeria monocytogenes

...owth medium BHI, supplemented with 0.5 M sucrose Washing solution 1 mM HEPES (pH 7.0), 0.5 M sucrose Electroporation solution 1 mM HEPES (pH 7.0), 0.5 M sucrose ...

Neurospora crassa

... Growth medium Vogels minimal medium, 2% sucrose Washing solution 1 M sorbitol; 1 M sorbitol, 1% PEG 4000 Electroporation buffer 1 M sorbitol, 1% PEG Outgrowth medium Vogels minimal medium...

Propionibacterium freudenreichii

... Washing solution Ice-cold 0.5 M sucrose Electroporation solution Ice-cold 0.5 M sucrose buffered with 1 mM potassium acetate (pH 5.5) Outgrowth mediu...

Pseudomonas aeruginosa

... Washing solution 300 mM sucrose Electroporation solution 300 mM sucrose Outgrowth medium LB medium Cuvette ...

Your Data: Use of pSilencer 1.0-U6 siRNA Expression Vector in the Analysis of Synaptotagmin Location and Function

... confirmed by using a continuous sucrose density gradient. Interestingly, studies performed in primary pancreatic endocrine cells revealed that Syt V is present in glucagon-containing islet alpha-cells but is not expressed in β-cell...

Preparative Nondenaturing Gel Electrophoresis to Purify NADP-Specific Glutamate Dehydrogenase From Chlorella, Rev B

...0 x g for 10 min and was combined with 3 ml of 40% sucrose and 1 ml of 0.02% Bromophenol Blue. ... polymerized in 39 mM Tris (pH 6.6), 12.7% sucrose using 0.07% riboflavin-5-phosphate (Bio-Rad) and 0.365 l/ml TEMED. Stacking gel polymerizati...

Whole Gel Eluter Purification of a Functional Multiprotein DNA Replication Complex, Rev A

...harose (Pharmacia), and following sedimentation in sucrose and glycerol density gradients, indicating that the ready co-purification of the proteins with one another was independent of nonspecific interaction with other cellular macromolecular components. 24 Native polyacrylamide ...

Single-Cell Complementation of Barley Mlo Mutants Using a PDS-1000/He Hepta System

... were harvested and kept on 1% agar containing 10% sucrose 4 hr prior to bombardment. Blumeria graminis f. sp. hordei K1 was propagated on H. vulgare cv. Golden Promise as previously described (Shirasu et al. 1999). Plasmids Plasmid pUGLUM (Zhou et al. 2001) carrying ...

Sub-Micron Gold Particles Are Superior to Larger Particles for Efficient Biolistic Transformation of Organelles and Some Cells

...pretreatment by placing the tissue on high sucrose media for 4 hr. prior to bombardment. Tissues were bombarded in the PDS- 1000/He with 1 or 0.6 gold particles coated with pBC17 (Goff, et al., 1990), a plasmid which encodes genes for the C1 regulatory gene and the B-Peru al...

Electroporation of Primary Bone Marrow Cells

... enzyme. The use of the phosphate buffered sucrose (272 mM sucrose, 7 mM PO 4 , 1 mM MgCl 2 , pH 7.4)...row or L929 cells. With phosphate buffered sucrose electroporation medium, the 25 F capacitor was used to produce a pulse with a time constant ...

Electroporation of Yeast Artificial Chromosomes

...y, therefore prior size fractionation with sucrose gradients to enrich for larger molecules, or treatment with spermidine to improve transformation efficiency of larger molecules, is required to obtain reasonable efficiencies with larger YACs. Several electroporation protocols...