spleen

Tissue Specificity for Mutation Parallels Tissue Specificity for Cancer

... Lung, leukemia, lymphomas Lung, spleen liver * De Boer et al., unpublished See text for additional details. TDBP The flame retardant tris(2,3-dibromopropyl)phosphate (TDBP) is an establishedkidney-specific carcinogen in male and female rats and male mice; in femal...

EppendorfPerfect gDNA Blood Mini Kit

...cterium is present in goat placenta (blood), mouse spleen or milk. Materials and Methods Sa...essentially of blood. Spleen (mouse): the spleen was homogenized in sterile physiological buffer. Milk (bovine) Blood: t...

EppendorfPerfect gDNA Blood Mini Kit

...cterium is present in goat placenta (blood), mouse spleen or milk. Materials and Methods Sa...essentially of blood. Spleen (mouse): the spleen was homogenized in sterile physiological buffer. Milk (bovine) Blood: t...

Amplification of Mouse cDNAs for Microarrays Using the Eppendorf MasterTaq Kit

... labeled cDNA One g of polyA+ RNA (mouse spleen and liver, respectively) were converted in...cDNA probes generated from mouse liver and spleen polyA+ RNAs The final experiment performed was to verify that the PCR products am...

Determinants of RNA Integrity and Purity

... in tissues with high cell densities, such as spleen or tissue culture cells. Figure 2 shows examples of gross contamination with genomic DNA. High molecular weight genomic DNA typically migrates as a broad, larger molecular weight peak that is well separated from rRNA peaks (Pa...

RNA, DNA, and Protein from a Single Sample

...use liver, kidney, brain, lung, heart, and spleen and from fresh cultured human cells using the standard TRI reagent protocol with either bromochloropropane (BCP) or chloroform (CHCl3). (A) Genomic DNA removal was assessed by one-step, (+) or () RT, quantitative PCR targetin...

RNAlater Around the World: The Art of Processing Difficult Samples

... temperatures for virus-positive spleen samples in RNA later or a glycerol/saline buffer. In both widely used diagnostic tests (RT-PCR and antigen-capture ELISA), viral detection was more sensitive in samples preserved with ...

The Fastest, Simplest, and Most Effective Way to Remove DNA Contamination

...th genomic DNA. An 8 g sample of spleen RNA was then digested for 15 min...ng Wild Type DNase I. RNA was isolated from mouse spleen using a procedure that results in significant contamination with genomic DNA. 8 g of...

The World's Best DNase Improved TURBO DNA-free

... enhancer. Consequently, we prepared a mouse spleen total RNA sample that contained 70% DNA an...0 fold with New TURBO DNA- free . Mouse spleen total RNA samples, highly contaminated with DNA (30% RNA and 70% DNA; 23 g), were treated wi...

Working With Laser Capture Microdissection Samples

... Real-Time RT-PCR of ICM Samples. Mouse spleen (A) and liver (B) samples (1 mg, 3 mg and 10 mg) were dissected by LCM and RNA was isolated using the RNAqueous-Micro Kit according to protocol. The RNA was then used as a template for the amplification of GAPDH by r...

Genomic DNA Preparation from RNAlater Preserved Tissues

...0 mg tissue samples. Mouse kidney, lung, and spleen genomic DNA have been successfully extracted with approximate yields of 70 g DNA per 100 mg tissue. Required Materials Razor blade or scissors ...

Cell Proliferation Assays

...ty of TopCount with increasing cell numbers, mouse spleen cells were cultured to a level of approximately 10...performed in another experiment on TopCount. Mouse spleen cells were cultured to approximately 10 6 cells/ml in a 96-well cell culture plate and stimulated w...