single
Navigation Links
single at biology technology
Human Primary Preadipocytes and Differentiated Adipocytes
... the altered expression stateallows the effects of single gene changes to be examined, which may modelmetabolic derangement associated with particular diseases. 9 To date we have successfully transfected Human Differentiated Adipocytes with the human adipose- specific obese gene and peroxiso...New Mammalian Two-Hybrid System Detects Protein-Protein Interactions
...This further underscores the limitations ofusing a single host organism to study eukaryotic proteins. Clearly, developing asystem to study protein-protein interactions using a mammalian host organismwould obviate many of these concerns and provide an attractive method foruncovering the biological role of ma...Low Abundance cDNA Cloned Using Stratagenes Human Universal cDNA Library
... final purified fraction showed the presence of a single protein of 65 kDa. 4 To identify ...cDNA. Both nonoverlapping probes hybridized to the single cDNA pool at position F6 on membrane D (Figure 2). The coordinate position (F6) deri...A Versatile Power Supply for All Electrophoresis Applications
... This versatile power supply has been designedas a single unit that can be used for any electrophoretic application. The broadfunctionality of the SmartPower power supply makes it ideal for both small andlarge laboratories. REFERENCES Blinderman, L., LeSchofs, B., and Sorge, J. (1991) Strategies...Highest Transfection Efficiency of an Endotoxin-Sensitive Mammalian Cell Line
...ning up to 350 g of high-purity plasmid DNA from a single midi-spin cupdevice. 1 Contaminating endotoxin may be easily removed by using thekits unique endotoxin removal buffer (ERB). Endotoxin, a common contaminantof plasmid DNA isolated from gram-negative E. coli hosts, is difficult toremove 2 ; its prese...Detect Single Nucleotide Sequence Changes with Mx4000 Molecular Beacon Allelic Discrimination Kits
...conformation, molecular beacon probes canrecognize single base differences, making them ideal for mutation d...lic discrimination kits , ff for the detection of single or multiple nucleotide changes in human genes. Thefive kits described in this article allow four sin...Antibodies for Studying NMDA Receptor Protein Expression and Synapse-Specific Immunolabeling
...re 1 shows that Anti-NMDAR2A recognizes a single band of approximately 180 kDa in NR2A-immu...obtained with Anti-NMDAR2B ( figure 2 ). A single band of 180 kDa is observed for NR2B-immunoprecipitated rat brain homogenate blotted with An...Effects of Thermal Cycler Well-To-Well Temperature Uniformity on PCR Results
...hermal electric modulesattached to the bottom of a single block for heating and cooling. However, thePeltier...ry made from human brain frontal cortex. A single PCR master mix was prepared large enough to accommodate six reactions for each of the two th...Brilliant Core Reagent Kits for QPCR and QRT-PCR
...ents for cDNA synthesis and PCR amplification in a single tube. This formatuses primers specific to the RNA ...inear hydrolysis (TaqManprobes), molecular beacon, single dye primers, and Scorpions fluorescenttechnologies. With the Brilliant QPCR core reagent kit, we det...Control RT-PCR Primers for Human Gene Transcripts with Varying Abundance
...dimportance in 1991 with the advent of the shotgun single pass, a partialsequencing of entire cDNA libraries...r each of the six RT-PCR abundance primer pairs. A single lambda library derived from the liver was used as template in all reactions ( Figure...Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells
...(1,030 fold). Coexpression ofboth receptors from a single dicistronic transcript from the vector pERV3facili...eterodimer family receptors, which require single nucleotide spacing between half-sites. Finally, the GEcR receptor was further modified by re...Innovative Tissue Array Technology for High-Throughput Screening of Gene Expression
...a collection of diverse tissuesamples mounted on a single slide. Proven successful in examining both normal ...ical in most research environments. In contrast, a single Complete View tissue array can contain all the major tissues and cell types of an an...Counting Colonies or Plaques Directly from Images of Agar Plates
...satellite colonies or contaminantsfrom a count. If single colonies are being counted multiple times, the minimumcolony size may need to be increased. If small colonies are unintentionallyexcluded from the count, the minimum colony size can be decreased. Image Acquisition In order to ensure the highest acc...Genotyping Single Nucleotide Polymorphisms with Molecular Beacons
...tagenes custom molecular beacons ff, to detect a single nucleotide polymorphism (SNP) in a region of chrom... was seen between the targets containing a single nucleotide difference. Distinguishing Three Genotypes Figure 2 illustr...New Temperature Cycler Features Interchangeable Blocks
...allows 200-l or 600-l tubes to be run on a single machine. Block sets are currently available in a 40-well and a 96-well format. In addition, a 384-well block set is in development and slated for a 1998 release. The blocks and paddle assemblies are easily and quickly exchange...Epitope-Tagging Vectors for Functional Analysis in Yeast
...itope-tagging analysis of two different genes in a single yeast cell. Characterizing the structure and function of every eukaryotic gene is anecessary task in the post genome era. Results of the yeast genome sequencingproject indicate that S. cerevisiae shows homologous sequences forone-third of the human ...Fast and Easy Isolation of PCR-Ready Genomic DNA from Whole Blood
...stion, and room temperature incubations. A single microspin cup is all that is necessary to carry ou... temperature, all steps are performed in a single microspin cup, and all isolations are easily completed in 40 minutes. An aliquot of 1 ml to ...Novel PCR Enhancing Factor Improves Performance of Pfu DNA Polymerase
...nger targets (e.g., >4-kb genomictargets) with single DNA polymerases. For example, Stratagene has devel...ymerase ( figure 2 ) or with other single PCR enzymes ( Figure 1 ). PfuTurbo DNA polymerase can be used routinely in all PCR a...Optimized Imaging of Protein Gels Stained with Coomassie Brilliant Blue Dye
...sualizingethidium bromide fluorescence. However, a single filter specifically designedfor ethidium bromide may be inadequate for use with other fluorophores orstains. In this report, we describe an optical filter designed for use withprotein gels stained with Coomassie brilliant blue dye. TheCoomassie blu...Efficiently Insert Unique Restriction Sites into Plasmid Vectors
...mple, accurate, and efficient. This method permits single andmultiple bases to be easily deleted and inserted into any double-strandedplasmid vector, eliminating the need for single-stranded DNA rescue andsubcloning into specialized vectors. The kit relies on high-fidelity PfuTurbo DNA polymerase,** which ...Pfu DNA Polymerase as a Hot Start Alternative
... The results from amplifying a 2-kb target from a single copy region of a transgenic mouse genome are shown ( figure 2A ). PCR amplifications used equivalent polymerase units of Taq DNA polymerase (alone or in conjunction with a Taq DNA polymerase-specific antibody or magnes...prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification
...rly useful when amplifying multiple targets from a single cDNAsynthesis reaction. In one-step RT-PCR proced...ingle-Tube RT-PCR system. Other kits marketed as single tube/singlebuffer RT-PCR kits are actually two-step kits since they require additionalcumbersome man...Production of Full-Length, Biologically Active MEK1WT and MEK1CA
...ere purified from crude cell extracts by a single GST affinity chromatography step, yielding about 4 mgs of purified protein per liter. The enzymes expressed and purified from S. pombe contained only full-length GST-MEK1 CA , which is about 66 kDa ( figure 1 , panel B...High-Efficiency Site-Directed Mutagenesis of Large Plasmid Vectors
...cult-to-mutagenize plasmid DNAtemplates using this single day mutagenesis method. The QuikChange XL kitfeatures components specifically designed for more efficient DNA replication andbacterial transformation, which are important when mutagenizing large templates.The QuikChange XL kit retains the simplicity ...A Simple and Rapid Technique to Purify High-Quality Midiprep DNA
...w up to350 g of plasmid DNA to be recovered from a single device. The resulting DNAcan be used for applicati...NA ( Figure 2 ). The binding capacity of a single StrataPrep midispin cup was determined by loading various known quantities of purified pBlue...A New PCR-based Mycoplasma Detection Method
...specifically amplifies an 874-bpPCR product from a single copy gene. In addition, the kit contains an intern...e sought to develop a PCR-based method to detect a single copy gene. In ourapproach, we identified a target gene whose evolution rate was sufficientlyrapid to...Restriction Endonucleases Undergo Rigorous QC Testing to Ensure Optimal Performance
...re 2 ). This conversion is indicative of a single nick in the RF I DNA. If greater levels of...which contains the lacZ reporter gene, is cut at a single position within the multiple cloning site using a five-fold excess of enzyme. A phen...Functional Cloning Using ViraPort Retroviral cDNA Expression Libraries
...which no selection applies other than that for the single focus-forming oncogene. Receptor Cloning ...rising a complex spectrum of mutantsderived from a single gene can be screened for dominant positive or negativemutations or for other altered or enhanced phe...Simple, Sensitive Isotyping of Mouse Monoclonal Antibodies
...identifying mouse monoclonal antibodyisotypes in a single step. Each IsoDetect test card is a self-contained assay,using colloidal gold-conjugated anti-mouse antibodies for isotype determinationof 0.1 g/ml of antibody in 5 minutes. For decades, antibodies have been used as key reagents for biologicalresear...A New Lambda Vector for Mammalian Expression
...e cloned insert DNA from lambda phage. When either single clones orthe entire lambda library is converted to a plasmid format by in vivo excision, 1-5 they combine the high-efficiency cloning of lambda vectors with the convenienceof plasmid libraries for functional studies. This elegant procedure requiresve...Fluorescence-Based Single-Tube Assays to Rapidly Detect Human Gene Mutations
...tance regulator (CFTR) gene, and the other two are single nucleotidesubstitutions in the coagulation factor ...reas of geneticresearch have revealed the value of single nucleotide polymorphisms (SNPs) inmedical research and clinical diagnostics. The demand is growing f...Versatile Reporter Vectors for Monitoring Viral Transduction
... of infection (MOI) and, thus, harbor only single copies of the hrGFP provirus, fluorescence over background was readily detectable with this vector. Table 1 Viral Titers* for pFB-hrGFP and pFB-Neo-LacZ vectors Vector Dilution ...Efficient Cleavage of Fusion Proteins to Yield Native Amino Termini
...ection of silver-stained PAGE gels after a single pass over calmodulin (CaM) affinity resin 4 (data not shown). A series of reactions containing various amounts of recombinant EK was incubated at room temperature for 21 hours, and portions of the reactions were analyzed by S...Expression and Purification of Recombinant Proteins That Have Native Amino Acid Sequence
...recombinant proteinsfrom crude cell lysates with a single pass through calmodulin affinity resin. 1,2 The CBP purification system is an excellent alternative to other affinity-tagsystems because of its gentle binding and elution characteristics. Anotheradvantage of this system is the small size of the CBP a...pFB-ERV: Retroviral Delivery of the Ecdysone Receptor Proteins
...cR.In stable cell lines infected with an estimated single copy of the receptorcassette, induction ratios of ... therefore, the colony theoretically has a single integrated copy of the receptor expression cassette. Conclusions The 200-fold induction se...Four New CastAway Precast Gels
...unique cDNA fragment, nondenaturing gels exhibit a single band whereyou would see several bands on a denaturing polyacrylamide gel. This meansthat nondenaturing gels are the ideal choice for many applications. 5.5% Long Ranger Gels Stratagene has joined forces with FMC Corporation, manufacturers of the Lo...Fractionating DNA Fragments Generated by Differential Display PCR
...l clones must be studied; in some cases, a single differentially expressed band may contain more than one DNA species. 4 Once the sequences of interest have been identified, the differential expression between the two RNA populations may be confirmed using an independent tec...Randomize Gene Sequences with New PCR Mutagenesis Kit
... moderate, or high mutation frequencies using a single set of optimal reaction conditions. The ki...and high mutation frequencies are achieved using a single set of optimal reaction conditions (MgCl 2 , balanced dNTPs). The only parameter var...Generate High-Quality, Directional Plasmid cDNA Libraries
...ow high-titerlambda libraries to be generated, and single lambda clones or entire Lambda ZAPlibraries can be rapidly converted into a plasmid format. 6-10 Conclusions Stratagenes new plasmid cDNA library construction kits are designed forgenerating high-quality, directional cDNA libraries in either the p...Unique DNA Polymerase Formulation Excels in a Broad Range of PCR Applications
... was developed to provide customers with a single PCR reagent to fulfill all PCR application needs. The Pfu-based Herculase formulation exhibits several unique features, including high fidelity, broad target-length capability, and superior amplification of extra-long targets ...High-Titer Retroviral Vectors for Gene Delivery
...,7 is avoided by coexpressing both proteins from a single transcript. In light of published reports showing that a significant increase in titer isachieved by minimizing the amount of extraneous sequence between the LTRs, 3,7 we also constructed the vector pFB, which contains only the sequences requiredfor...TKB1 Cells Identify Receptor Tyrosine Kinase Interacting Proteins
...dcellular extracts. Fusion proteins that contain a single tyrosine residue can beused to map sites of interaction; and interaction sites can be identified which,when mutated in vivo, lead to a loss of protein activity or stability. Forexample, when two tyrosine residues mutate within the juxtamembrane regio...Tools for Detecting MSH2 Expression in Chinese Hamster Ovary Cells
...reactions, according to instructions. The expected single 0.9-kb bandwas generated. One of the reactions was purified using the StrataPrep PCRpurification kit, and the other was purified with the StrataPrep DNA gelextraction kit. 7 Each of these fragments was labeled with a- 32 P-dCTPin a 5-minute reaction...Keys to Successful Densitometry
...s rapid comparison between bands or spots within a single image fromthe Eagle Eye system. figure 1 Determining integrated density with Eagle Sight software begins with choosing the Integrated Density function from the toolbar or Analyze menu ( figure 1 (30k), RED...Mammalian Expression Vectors with Interchangeable Selectable Markers
...ions Stratagenes Exchanger vector system allows a single expression construct to be used for multiple applications without subcloning the gene of interest. This system features the pExchange core vectors into which, through Cre-mediated site-specific recombination, hygromycin-, puromycin-, or neomycin-resi...Tissue Specificity for Mutation Parallels Tissue Specificity for Cancer
... that from the kidney included unique deletions of single GC base pairs.Thus, in this case, TDBP-specific mutations were induced only in the tissuewhere tumors were recovered. Hence, DNA sequencing can improve the assaysensitivity in situations where the induced mutant frequency is low. DMN Tissue specifi...Easy Cosmid DNA Purification with StrataPrep Kits
... g, respectively, of high-purity plasmidDNA from a single spin cup. 1,2 These kits can also easily purify verylarge plasmids, including cosmid DNA. To demonstrate cosmid purification, a SuperCos I clone containing a 40-kb insert from human chromosome 11 was isolated from two, 100-ml overnigh...Sequence-Validated and Expression-Tested Human cDNA in a Dual Expression Vector
...nce-validated and expression-tested human cDNAin a single dual- expression vector. These clones eliminate th...ree copies of the c-myc epitope tag, and a single copy of the HIS6 purification tag ( Figure 1). The c-myc epitope is derived from the human c...Stratagenes Human cDNA Microarrays: Perform Gene Expression Profiling and Discover New Genes
...end of each of these cDNAs have beensequenced in a single pass. The sequences were compared to those in the ... insert-specific primer, the presence of a single PCR product of the correct length is confirmed by gel electrophoresis. If the cDNA template ...Fast Isolation of Total RNA from Small Numbers of Cells
...hanol precipitation. DNA is removed with a single DNase I incubation step directly on the matrix, the matrix is washed, and pure RNA is eluted with a small volume of buffer. The recovered RNA is then ready to use. The StrataPrep total RNA microprep kit provides all necessary ...New PCR Primers for Human Diseases
...n figure 1 . Each amplification yielded a single product of the appropriate size in reactions with DNA from infected serum samples ( figure 1 and figure 2 ), whereas amplification products were not detected in any of the negative controls, uninfected serum samples ...Mx4000 Multiplex Quantitative PCR System*,PPP
.... PCR amplification anddetection are combined in a single step, speeding the process of gatheringexperimental data. The QPCR process uses a single closed tube, which eliminatesthe need for numerous post-PCR manual steps, and reduces the possibilit......und to the matrix, was then submitted to a single wash for the removal of contaminants and eluted with 70 l of Molecular Biology Grade Water 8 as described in the protocol. Results and Discussion Comparison of Bacterial Cultures in 15 ml Conical Tubes and LidBac ...Intracytoplasmic Spermatocyte Injection (ICSI)
...gnancies after intracytoplasmic injection of single spermatozoon into oocyte. Lancet 340:17-18 [4] Van Steirteghem AC, Liu J, Joris H, Nagy Z, Janssenswillen C, Tournaye H, Derde M-P, Van Assche E, Devroey P (1993) Higher success rate by intracytoplasmic sperm injection...Sample Preparation for Microinjection
...jections which can be performed with a single capillary, the injection substance should be centrifuged for 1015 minutes at a minimum of 10,000 x g before back loading the capillary. As the cross section of the capillary tip is small and very little liquid woul......al suture We performed 2030 single experiments per day. Each of these experiments included the mounting of a pipette into the pipette holder, subsequent lowering into the experimenting chamber with the HOME function and the positioning of the pipette (diameter of ope......lls can then be cultivated further or analyzed via single cell PCR*. ... microorganisms from mixed cultures by means of single cell PCR is greatly reduced. Microorganisms present in low numbers can also be ex...Principles and applications of electrical cell fusion
...s fusion products are isolated and selected. These single cells are replicated as clones, which is why the term monoclonal is used. The fusion products, described as hybridoma, stand out for two reasons: they secrete a specific antibody and they can be replicated in culture ......, milk and fecal samples by the use of a single touchdown polymerase chain reaction. Vet Microbiol 72 (3-4): 285-93. Hoover, T.A., Vodkin, M.H. and Williams, J.C. 1992. A Coxiella burnetii repeated DNA element resembling a bacterial insertion sequence. J Bacteriol ......s fusion products are isolated and selected. These single cells are replicated as clones, which is why the term monoclonal is used. The fusion products, described as hybridoma, stand out for two reasons: they secrete a specific antibody and they can be replicated in culture ...Other ContentschemiosmoticcentrosomecentrosomecentrosomesquaresquaresquaresquaresquaresquaresquarechichlorophyllchlorophyllchlorophyllchloroplastchloroplastchloroplastchromatinchromatinchromatinchromatinchromatinchromatinchromatidchromatidOther TagsAnimalsAnimalsFasterFasterFasterBehavioralFormationFormationFormationFormationFormationButterfliesAggressionAggressionAspirinLacticFertilityFertilityTicksConcern
(Date:7/24/2008)...08. A fire is currently burning through a study ar... about 2 years ago. Managers used data and analysi... study in the planning, analysis, and implementati...is now active., The Gotchen LSR, lies on the eas...vers about 15,000 acres of the Mount Adams Ranger ...
(Date:7/24/2008)...ave reduced carbon storage in western U.S. forests...tieth century has caused a widespread increase in ...ity of stems growing on trees within western U.S. ...s and are thought to account for much of North Ame...aboveground biomass, Fellows and Goulden compare C...
(Date:7/23/2008)...8 One of the reasons people on low-carbohydrate d...ake of fructose, a type of sugar that can be made ...t UT Southwestern Medical Center. , Dr. Elizab...on and lead author of a study appearing in a curre...eam,s findings suggest that the right type of carb...
(Date:7/23/2008)...s living in three estuaries on the Pacific coast o... determined that biomass of these parasites exceed...n 20 times. , Their findings, which could have s... appear in this week,s issue of the journal Natur...t exists in a given habitat. It is expressed eithe...
Breaking Biology News(10 mins):Landscape study may offer solutions for fire managers 2AGU journal highlights -- July 23, 2008 2AGU journal highlights -- July 23, 2008 3AGU journal highlights -- July 23, 2008 4AGU journal highlights -- July 23, 2008 5AGU journal highlights -- July 23, 2008 6AGU journal highlights -- July 23, 2008 7AGU journal highlights -- July 23, 2008 8Limiting fructose may boost weight loss, UT Southwestern researcher reports 2Limiting fructose may boost weight loss, UT Southwestern researcher reports 3Parasites outweigh predators in Pacific Coast estuaries 2Parasites outweigh predators in Pacific Coast estuaries 3MU researchers enhancing motion capture technology to benefit older adults 21529 1MU researchers enhancing motion capture technology to benefit older adults 21529 2Live a Longer and More Peaceful Life With Chant Music For The Soul 21524 1Live a Longer and More Peaceful Life With Chant Music For The Soul 21524 2SAIC to Webcast Presentation at William Blair Growth Stock Conference 21519 1SAIC to Webcast Presentation at William Blair Growth Stock Conference 21519 2Herbalife Sued for Negligence and Fraud by Victim 21516 1Herbalife Sued for Negligence and Fraud by Victim 21516 2(Date:7/25/2008)... - 180-Days of Marketing Exclusivity Begins.../ -- Watson,Pharmaceuticals, Inc. (NYSE: WPI ), a...ed today that its subsidiary has commenced commerc...sules USP product in the 40mg,strength from its Da...n,with the launch, Watson has 180 days of marketin...
(Date:7/25/2008)...k to benefit ALS Therapy Alliance to be presented ...ght, WOONSOCKET, R.I., July 25 /PRNewswire/ -- CV...aised a record-breaking $4.4 million in just 21 da...n for ALS research. This is,the seventh year of th...ised more than $15 million for ALS research. This ...
(Date:7/25/2008)... The Retina Group of New York wil...ated macular degeneration especially designed for ...le Public Library at 116 Merritts Road, Farmingdal...5. Attendance is free and refreshments will be pro...16) 939-6100. , Hicksvill...
(Date:7/24/2008)...STON-SALEM, N.C. Researchers at Wake Forest Univ...g-standing clinical belief that the hepatitis C vi... restore itself after HIV patients are treated wit... , Hepatitis C (HCV) infection is more serious i...ge, according to the Centers for Disease Control. ...
Breaking Medicine News(10 mins):Health News:Watson Launches Omeprazole Delayed-Release Capsules, 40MG 2Health News:CVS/pharmacy Raises Record-breaking $4.4 Million for ALS Research Through Annual ALS Fundraising Campaign 2Health News:Retina Group of New York To Present Seminar on Age-Related Macular Degeneration; The Leading Cause of Visual Loss in Seniors 2Health News:Retina Group of New York To Present Seminar on Age-Related Macular Degeneration; The Leading Cause of Visual Loss in Seniors 3Health News:Researchers disprove long-standing belief about HIV treatment 2Health News:Researchers disprove long-standing belief about HIV treatment 3[0] Watson Launches Omeprazole Delayed-Release Capsules, 40MG[0] CVS/pharmacy Raises Record-breaking $4.4 Million for ALS Research Through Annual ALS Fundraising Campaign[0] Retina Group of New York To Present Seminar on Age-Related Macular Degeneration; The Leading Cause of Visual Loss in Seniors [0] Researchers disprove long-standing belief about HIV treatment
AnimalsFasterBehavioralFormationExpeditionHoneybeeButterfliesSpousalAggressionAspirinLacticFertilityTicksConcern
Other Tagsinsisted 2insisted 3insisted 4insisted 5pricing 2enables 2enables 3enables 4enables 5enables 6enables 7enables 8enables 9enables 10enters 2enters 3happens 2happens 3happens 4happens 5happens 6happens 7happens 8happens 9happens 10activity 2activity 3activity 4activity 5activity 6activity 7activity 8activity 9activity 10ordinary 2training 2training 3training 4training 5facing 2facing 3| Copyright © 2003-2008 Bio-Medicine. All rights reserved. ABOUT | CONTACT US | DISCLAIMER | PRIVACY POLICY | TERMS AND CONDITIONS |  |