reproductive

Innovative Tissue Array Technology for High-Throughput Screening of Gene Expression

...glandular tissue vascular system reproductive system Preserved Tissue Integrity We assessed the morphological integrity of tissues and the antigenicity of proteins by assaying the Complete View array slides in hematoxylin and eosin staining and immunohistochemical ...

Intracytoplasmic Spermatocyte Injection (ICSI)

...uples worldwide. Since its introduction into reproductive medicine in 1978 [1] , in vitro fertilization has been considered a successful method of treating tubal sterility. With increasingly intensive diagnosis of both partners, certain aspects of male subfertility are coming to the fore...

Efficient Transfection of Neurospora Crassa

... INTRODUCTION: The diverse reproductive capabilities and the nutritional conventions of Neurospora crassa have made this filamentous fungus a model organism in the field of molecular biology and genetics for more than a half a century. In fact, N. crassa was used in...

Efficient Transfection of Neurospora Crassa

... INTRODUCTION: The diverse reproductive capabilities and the nutritional conventions of Neurospora crassa have made this filamentous fungus a model organism in the field of molecular biology and genetics for more than a half a century. In fact, N. crassa was used in...

Determinants of RNA Integrity and Purity

...all RNAs are most often found in RNA isolated from reproductive and intestinal tissues. Their fluorescence can sometimes dwarf that of the rRNAs. Generally, they can be distinguished from degradation products because the rRNA ratio of the sample will be greater than 1.0 and the bas...

MicroRNA Profiling by Array Analysis Reveals Critical BioMarkers

...ilar, digestive tract tissues cluster, and reproductive organ tissues are similar (Figure 4). Interestingly, the brain miRNA profile is clearly distinct from the other tissues that were analyzed. Approximately 30 miRNAs are expressed primarily in 1-3 related tissue types. Interesti...

Highly Sensitive microRNA Array Performance

... digestive tract tissues clustered, as did that of reproductive organ tissues. Interestingly, the brain miRNA profile was clearly distinct from the other tissues analyzed. The observation that adult tissues possessed unique miRNA profiles suggests that miRNAs function in tissue differentia...

New Automated Yeast Cell Counter Ends Tedium & Errors of Manual Counting

...lve or dissociate cells that are in the process of reproductive division. Vortex or pipette vigorously for a few seconds to mix. The total volume is now 500 μL, with a 10-fold dilution. If necessary, repeat the dilution process to yield a sample 1 x 10 5 cells/mL to 2 x 10 6 cells/mL. (In general, a 100 to...

ESR1 Gene Expression in Oncology and Metabolic Diseases Using the ASCENTATM System Target Validation and Identification of Novel Disease Indications

...firms published gene expression results for female reproductive system and liver cancers, but also reveals novel f...play the highest expression levels of ESR1. Female reproductive tissues (uterus, fallopian tube, breast and ovary) have the highest degree of ESR1 expression, follo...

Biologist who cloned Dolly the sheep believes therapeutic cloning will gain acceptance

...eus. While it can be used as the first step toward reproductive cloning, which is banned in the United States, it ...orts cloning for therapeutic purposes, but not for reproductive purposes, vetoed the bill. Ethical explanation Wilmut, who was in Madison in part to lecture an...

National Academies induct five UW researchers

... policy on biotechnology, stem cells, cloning, and reproductive technologies. Barry Ganetzky , a professor of genetics, who is best known for his studies of how cells generate and propagate electrical impulses in the nervous system. His work has contributed to the understanding of human neurological disorders, ...

Doyle urges Senate to loosen stem cell restrictions

...l, Doyle vetoed a bill that would have banned both reproductive cloning and therapeutic cloning. The latter, which involves the transfer of genomic DNA for cell transplantation therapies, is of interest to university researchers but raises the ire of opponents because an embryo is destroyed in the isolation of st...

Doyle, Green exchange stem cell salvos

...elopment.) Therapeutic cloning is distinct from reproductive cloning, where nuclear transfer is used to produce a new individual. The bill that made it out of the Legislature would have banned both. Relentless opponent? Citing his record in Congress, Doyle characterized Green as a relentless opponent of st...

A brief timeline of the stem cell debate

...ournal, Science . The embryos were cloned not for reproductive purposes but as a source of stem cells. The news reopens the contentious debate over somatic-cell nuclear transfer, which is sometimes referred to as therapeutic cloning. Scientists say cloning offers a unique way to produce cells that may someday be...

Federal and global guidelines on stem cell research offer a level playing field

...donors, (2) from excess embryos created solely for reproductive purposes, and (3) without any financial inducements to the donors. To a "t," that describes the stem cell lines created through the UW-Madison and its private, non-profit affiliates, the Wisconsin Alumni Research Foundation and WiCell. Wisconsin re...

Standing up for stem cell research

...ates disguised the bill as an attempt to ban human reproductive cloning - something that everyone agrees is unacceptable, and is already prohibited by the federal government. The real purpose of this bill was to limit stem cell research in the name of far right-wing ideology. I think any parent would agree that...

Doyle vetoes cloning ban

...onsin. The bill would outlaw not only cloning for reproductive purposes but also what proponents call therapeutic or research cloning, in which an embryo is created with identical DNA as an original subject for the purposes of harvesting stem cells during the first several days of development. The embryo is dest...

Separating science fiction from reality in the human cloning debate

...e Legislature passed a bill that only banned human reproductive cloning, Doyle would have signed it in a nanosecond. Other than a tiny number of weird scientists, its hard to find anyone who likes the idea of implanting a cloned embryo into a womans womb, risking not only the health of the mother but almost certa...

WiCell to host new national stem cell bank

... legislature passed a bill to ban cloning for both reproductive and research purposes. While scientists agree with the ban on reproductive cloning, they disagree with legislative opposition to cloning for research purposes. Such research i...

Wisconsin Senate votes to outlaw cloning; measure still faces veto

...he bill, AB 499, would outlaw not only cloning for reproductive purposes but also what proponents call therapeutic...ion," Wiley said. "The amendment would have banned reproductive cloning but kept open an area of research that is not only scientifically promising, but holds out h...

Amendment on cloning ban rejected; vote on bill set for Wednesday

...on Wednesday. It would outlaw not only cloning for reproductive purposes but, also, what proponents call therapeut... of cloning. "To confuse therapeutic cloning with reproductive cloning, it does science a great disservice and it does the Legislature a great disservice," said Se...

Cloning ban comes before Wisconsin Senate on Tuesday

...known as AB 499, would outlaw not only cloning for reproductive purposes but also what proponents call therapeutic...as supported an amendment to differentiate between reproductive human cloning which would result in the creation of a fully formed, live human being and therapeut...

Illinois stem cell funding could aid issue's visibility

...od and embryonic stem cell research but denied for reproductive cloning efforts. Blagojevich's use of executive power was controversial, particularly among stem cell research opponents, according to reports out of Illinois. But Jim Leonhart, executive vice president of the Wisconsin Biotechnology and Medical De...

The Attack of the Clones: No, its not a summer movie

...suggest the process for cloning a human embryo for reproductive purposes is any different at the outset -- from t... some Republicans voted to separate the issue into reproductive cloning and therapeutic cloning. The vote in the state Senate if it happens at all may be much cl...

Cloning ban passes Assembly, Doyle pledges veto

... bill would outlaw the cloning of embryos both for reproductive purposes and for what practitioners have called research or therapeutic cloning, the making of a cloned embryo for the purpose of extracting stem cells. Penalties for violating the bill if it were to become law would go up to ten years in prison and ...

Cloning ban too broad, stem-cell researchers argue

...ban if approved, would outlaw not only cloning for reproductive purposes, but also what proponents call therapeuti... to the bill that would make a distinction between reproductive cloning and research cloning, with the former outlawed and the latter still permitted. It has yet to...

With ethics guidelines, political consensus emerging on stem-cell research

... doctors to create more embryos than necessary for reproductive treatments. Human embryos used for research should not be grown in culture longer than 14 days, the point at which the body axis and central nervous system begin to form. Each institution involved in stem cell research should create an advisory boa...

UN committee opposes human cloning

...or purposes of research and has no law prohibiting reproductive cloning or IGM. Charo feels that the United States... legislation that would prohibit human cloning for reproductive purposes but permit the creation of cloned embryos or development of human embryo farms for research...

Human cloning plans get blessing from British government

... create fully developed human clones. This is not reproductive cloning in any way, said Wilmut. The eggs we use will not be allowed to grow beyond 14 days. Once the stem cells are removed for cell culture the remaining cells will be destroyed. The embryonic stem cells that we derive in this way will only be used...

Infertility linked to lawn chemicals by Marshfield Clinic researcher

... amount of chemical(s) dangerous to an individuals reproductive health, a cautious approach seems best, Greenlee said. Women considering or trying to conceive should make every effort to minimize their exposure to lawn-care and agrochemical products, Greenlee said. Applying these products according to label guid...

UW Madison launches new stem cell research program

... researchers in disciplines as diverse as anatomy, reproductive physiology, biological engineering, surgery and neurology. The daylong event attracted 140 people. Lewis likened the new initiative to something akin to what goes on in a lab dish populated with stem cells: "Embryonic stem cells don't like to be alo...

Public Hearing Held for controversial anti-cloning bills

...thical concerns about cloning, whether it be human reproductive cloning or therapeutic cloning," Still said. "I think there are ethical concerns on the balance side if therapeutic cloning is barred." --------------------------------------------------------------------------- Lincoln Brunner is a Stevens Point, ...