Repressor in Biological Technology

JCI table of contents: April 22, 2008

... by a reliance on either cyclin D1 or cyclin D2, such as many individuals with multiple myeloma. TITLE: Identification of kinetin riboside as a repressor of CCND1 and CCND2 with preclinical antimyeloma activity AUTHOR CONTACT: A. Keith Stewart Mayo Clinic, Scottsdale, Arizona, USA. Phone: (480) ...

Functional Cloning Using ViraPort Retroviral cDNA Expression Libraries

... 2.2 Transcriptional repressor of known cell-cycle regulatory protein (to be discussed in a ... 13. Onco-1 contained a gene encoding a transcriptional repressor of a known cell cycle regulatory protein. It is not surprising, ...

Sequence-Validated and Expression-Tested Human cDNA in a Dual Expression Vector

... features designed for inducible high-level protein expression in bacterial cells. The vector contains the hybrid T7/lac O promoter and lac repressor gene (lac I) to regulate protein expression. Therefore, expression is inducible using isopropyl-1-thio-b-D-galatopyranoside (IPTG) in BL21(DE3) ...

Mammalian and Bacterial Expression in One Vector

... cells. Expression of heterologous genes in bacteria is regulated by a hybrid T7/ lacO promoter. The pDual vector was engineered to carry the lac repressor gene (lacI). Expression is therefore inducible using IPTG in bacteria that contain the T7 RNA polymerase. ## In both bacterial and mammalian ...

pFB-ERV: Retroviral Delivery of the Ecdysone Receptor Proteins

... repressed until ligand binding, at which time high-level transcriptional activation occurs (i.e., the heterodimer is converted from a tight repressor to a trans-activator). Using the Complete Control plasmid-based system, induction ratios of greater than 1,000-fold have been achieved in both ...

Expression and Purification of Recombinant Proteins That Have Native Amino Acid Sequence

... released by Stratagene. 1 It is derived from the pET-11 vector series and contains the lacI q gene for expression of the Lac repressor protein and the hybrid T7 promoter for controlled expression of the inserted protein coding sequence. 7,8 The vector is ...

High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli

... released by Stratagene. It is derived from the pET-11 vector series and contains the lacI q gene for expression of the Lac repressor protein and the hybrid T7 promoter for controlled expression of the inserted protein coding sequence. 8 The vector is ...