recombination

Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System

...ransferredinto the adenoviral genome by homologous recombination in E. coli. Thismethod allows recombinant adenovir...e into the adenovirus genome bymeans of homologous recombination in vivo. 2 To isolate and identifyrecombinant adenovirus by this method, multiple plaque isolations...

Safe Production of High-Titer Retrovirus

...ation-competent retrovirus (RCR) due to homologous recombination betweenthe vectors. 1 Although the relative spee...plication-competent retrovirus (RCR) by homologous recombination is greatly reduced. Compatibility: Compatible selectable markers allow generation of stable ...

Easily Amplify Genomic DNA with Long-Distance PCR

...sive modifications, eitherintroduced by homologous recombination or naturally occurring (i.e.,deletions and insertions). REFERENCES Abbott, C., et al. (1988) Trends Genet. 4: 325. Hanley, T. and Merlie, J.P. (1991) Biotechniques 10: 56. Winberg, G. (1991) PCR Methods Appl. 1: 72-74. ...

Mammalian Expression Vectors with Interchangeable Selectable Markers

...nsertion is carried out in vitro by a Cre-mediated recombination of LoxP sites, and recombination products are selected via drug resistance. The components of the Exchanger system include Cre recomb...

Transgenic Animals

...target of this process is homologous recombination with the chromosome of the cell, i.e. introduction or exchange of DNA at one location with homologous DNA sequences. Following transfection, the descendants of one individual cell (clone) are analyzed using m...

Embryonic stem cells, mouse

...ected clones originating from homologous recombination by PCR or Southern Blot analysis. Detection methods for transfection: Selection with G418 to identify G418 resistant, stably transfected colonies carrying the neo R marker of the linearized targeting vector...

The use of TaqMaster PCR Enhancer

... ES cells that are positive for a homologous recombination event are then transplanted into blastocyst stage embryos in order to generate chimeric mice. In most cases, gene targeting is used to introduce mutations into specific genes in the mouse genome. The transgen...

QIAGEN Multiplex PCR Handbook

... Kit and a set of 3 primers specific for the recombination activating gene 2 locus. The primers were designed to generate PCR products of different sizes so that wild-type mice (wt) could be easily distinguished from heterozygote mutant mice (ht), or homozygote mutant mice (hm). ...

QIAGEN Multiplex PCR Kit

...) mice. A Using a primer set for the recombination activating gene 2 locus. B Using a primer set for the interferon- V gene locus. M : markers. (Data kindly provided by S. zur Lage and S. Weiss, National Research Center for Biotechnology, Braunschweig, Germany.)...

Custom and library siRNA for efficient gene silencing

...nt (hm) mice. A Using a primer set for the recombination activating gene 2 locus. B Using a primer set for the interferon- Ygene locus. M : markers. (Data kindly provided by S. zur Lage and S. Weiss, National Research Center for Biotechnology, Braunschweig, Germany.)...

Expand Long Template PCR System

...by amplifying cDNAs Analyzing homologous recombination events Characterizing cloned sequences carried in lambda-phage Amplifying full-length cDNAs or long fragments of cDNA (RACE) Expand Reverse Transcriptase is a genetically engineered version of M-MuLV Reverse Tr...

Efficient Cloning and Electro-transformation of Large Eukaryotic DNA Fragments

...siderable body of knowledge concerning its recombination pathways. In several instances the instability of foreign DNA in E. coli has been overcome by the judicious choice of appropriate, multiply marked, recombination-deficient strains. 911 References 1. Burke, ...

Homogeneous, chemiluminescent kinase assay kits for assessing the functional activity of a broad range of serine-threonine and tyrosine kinases

...ay principle EFC technology is based on the recombination of two inactive β-galactosidase (β-gal) fragments; complementation of the enzyme donor (ED) fragment and the enzyme acceptor (EA) fragment forms the active tetrameric β-gal (Fig 1). Subsequent hydrolysis of luminescent substrate genera...

SNPs Powerful Tools for Association Studies

... distance between two markers is a function of the recombination frequency the higher the recombination frequency, the farther apart two markers are on a genetic map. The unit of measure for genetic dista...

Measurement of Green Fluorescent Protein in the SpectraMax Gemini XS Spectrofluorometer (MaxLine Application Note #44)

...survival rates 7 or measurement of homologous DNA recombination repair which plays a role in cell viability/genomi... host cell reactivation assay to assess homologous recombination capacity in human cancer cell lines. Biochem. Biophys. Res. Commun. , 281(1) : 212219. bac...