rabbit

Antibodies for Studying NMDA Receptor Protein Expression and Synapse-Specific Immunolabeling

...Stratagene introduces an affinity-purified rabbit polyclonal synapsin antibody, Anti-Synapsin I, that can be used for studying synapsin expression in different cell types and brain regions. This antibody is suitable for studies of synapsin expression in a variety of different...

Simple, Sensitive Isotyping of Mouse Monoclonal Antibodies

...es a colloidal-gold conjugation technique to label rabbit anti-mouse l and k light chain antibodies as indicating reagents, and rabbit anti-mouse isotyping immunoglobulin coated on a membrane. When a specific mouse immunoglobul...

Enzyme Immunoassay for Studying Intracellular Levels of cAMP

... a cAMP-peroxidase conjugate that binds to rabbit anti-cAMP-specific antibodies. ...te with a fixed quantity of tracer for the rabbit anti-cAMP-specific antibodies. All unbound reagents are removed by a wash step. The reaction...

Detect Released Oligosaccharides Using the Eagle Eye II Still Video Imaging System

...ingerMannheims DIG Glycan Differentiation kit. The rabbit polyclonal antibody(IgG) was raised against human P-glycoprotein in our lab. Sample proteins weretreated with PNGase F (Bio-Rad kit) to release all N-linkedoligosaccharides, following the kits instructions. All glycans were labeledwith ANTS using red...

Effectene Transfection Reagent

...sion of green fluorescent protein (GFP) in primary rabbit aortic smooth muscle cells transfected using Effectene Reagent. 1 x 105 cells were seeded one day prior to transfection, and transfections were performed in 6-well plates using 0.4 g of a GFP-reporter plasmid and 10 l ...

EndoFree Plasmid Kits

...cells Primary rabbit gastric parietal cells were transfected with pEGFP-N2 (CLONTECH) prepared by the methods indicated. Transfections were performed using Effectene Transfection Reagent. The data represent the percentage of cells expr...

In Situ Cell Death Detection Kit

... This figure shows apoptotic cells in rabbit endometrium. The In Situ Cell Death Detection Kit, Fluorescein was used for staining a paraffin-embedded tissue section, which was then analyzed under a fluorescence microscope....

Delivery of pCMV-S DNA Using the Helios Gene Gun System Is Superior to Intramuscular Injection in Balb/c Mice

...1 hr. Following five more washes, 100 l of 1:1,000 rabbit anti-mouse-HRP (Serotec Ltd, Oxford, UK) i...med as described above with the exception that the rabbit anti-mouse HRP antibody was replaced with 100 l of 1:1,000 goat anti-mouse IgG 1 -HR...

Quantitative Analysis of Protein-DNA Associations in Vivo Using Real-Time PCR, Rev A

...trol sample immunoprecipitated with normal rabbit serum. Fold differences (Pdx1 ChIP relativ...1 chromatin immunoprecipitated with normal rabbit serum and NIH 3T3/EV chromatin immunoprecipitated with Pdx1 antiserum or normal rabbit serum...

Multiplex protein detection with the ECL Plex fluorescent Western blotting system using the Ettan DIGE Imager

... 9211S (Thr180/Tyr182) Antibody, rabbit (Cell Signaling) 10x PBS (Medicag...<P >The blots were then incubated with the rabbit anti–human transferrin or the mouse anti–actin primary antibody (1:750 dilution in PBST)...

Utilization of Donkey Anti-Rabbit PS SPA Imaging Beads in the quantitative measurement of cAMP in cells

... anti-rabbit antibody enable the immobilization of rabbit secondary antibodies during the assay. The Donkey Anti-Rabbit PS SPA Imaging Beads bind to the Fc region of the secondary antibody, leaving the antigen binding Fab region unhindered. Application data is demonstrated here for the utilization of Donkey...

Caspase-3 Activation - An Indicator of Apoptosis in Image-Based Assays

...re then incubated with cleaved caspase-3 (Asp 175) rabbit polyclonal antibody (Cell Signaling Technology, 9661, 1:100 dilution) in 5% goat serum and 0.3% Triton X-100 over night at 4C. Further wash steps removed unbound antibody prior to the addition of the secondary Alexa 488 goat anti-rabbit antibody (Inv...

DNA Damage and Repair - Quantification of Sub-Cellular Events Using Automated Confocal Imaging

... was used. 53BP1 was detected using the anti-53BP1 rabbit polyclonal antibody (Novus Biologicals, NB 100-304) diluted as described for H2AX. After 1h at room temperature, the primary antibody was removed and cells were incubated with Tween 20 (0.05% in PBS, 10 minutes). Two PBS washes prepared the ce...

Quantitation of Oxybutynin in Rabbit Plasma Using the Varian 1200L LC/MS/MS System

... by absorption through the urethra. Using a rabbit model, urethral suppositories, formulated with oxy... Sample Preparation A 0.5 mL aliquot of rabbit EDTA plasma containing oxybutynin chloride is spiked with 10 μL of diphenhydramine (0.5 ng/mL) ...

Endotoxins and their Relevance in R&D ApplicationsLPS Detection & Removal Methods

... in the seventies and replaces more and more the rabbit pyrogenic test which was used since 1940. ...ee" has to be checked. The advantage of the rabbit test (Ph. Eur. 2002, 2.6.8) is that all known and unknown pyrogenics can be detected. Theref...

Why are new drugs failing?

...ardio tox studies The isolated heart model uses a rabbit heart model to determine the effects of new drugs on coronary vasculature and cardiovascular pump function. CorDynamics looks at the left ventricular function, vascular resistance and myocardial contractility. The company's initial labs are small j...

The IT drug Just say no?

...ion of most modern IT projects. The business-like rabbit rushing by, very late for a very important date, a...ways just out of reach around the next turn of the rabbit hole or opportunity, and unavailable for any meaningful dialog. The falling, tumbling, almost halluc...

An entrepreneurial success story Frank Langley champions biocapitalism

... during the World Wars because unlike other meats, rabbit meat was not rationed. As time went on, Pel-Freez ...gers realized that the by-products from processing rabbit meat things like rabbit tissues, organs, and blood could be used for testing and experimental purp...

The Year Ahead in IT

...und's term. There's just not enough time to pull a rabbit out of the hat, however, and venture capitalists in all but the top tier are in for a world of hurt. 3. Expect the re-emergence of the Consumer Market. LAST YEAR: The consumer market will get a polished image in 2003, driven largely by initiat...

OpGen Database Helps to Reduce Bioterrorist Threat

...agent of Tularemia, a disease often referred to as rabbit or deer fever. The bacterium is naturally found in rodents, rabbits and hares in the United States. If F. tularensis was used as an airborne biological weapon, without treatment people could experience severe respiratory illness, including life-thr...