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A New C-Terminal GST Vector for Protein Production in S. pombe
...g the posttranslationalmodifications of eukaryotic proteins that may be critical for their structureand functi...sirable since the stability and activity of fusion proteins may vary with thelocation of the tag. 6-8 In addition, by tagging a protein at theC-terminus, only ...New Mammalian Two-Hybrid System Detects Protein-Protein Interactions
...ers to study interactions in vivo betweenmammalian proteins that require posttranslational modification or ext...partner to the other domain,interaction of the two proteins results in restoration of the activation of thetranscriptional activator. Genetic selection for this...Assess the In Vivo Activation of Signal Transduction Pathways with PathDetect Reporting Systems
...protein kinases, phosphatases, and otherregulatory proteins have been cloned. Many of these gene products are ...ll allow the in vivo function or activity of these proteins tobe determined. Stratagenes PathDetect in vivo signal transductionpathway reporting systems are ...Low Abundance cDNA Cloned Using Stratagenes Human Universal cDNA Library
...mRNA. Purification of proteins and identification of their cognate cDNAs are powerful tools for studying the molecular mechanisms underlying biological systems. Highly sensitive mass spectrometric techniques provide valuable peptide sequence information fro......ive celldivision. The ability to overexpress human proteins in human cell linesallows purification of recombinant proteins with the correct posttranslationalprocessing. Additionally, high titers (amplified titers up to 10 1...An Epitope Tagging Vector for Gene Expression in Mammalian Cells
...ntify associated proteins, track movement offusion proteins within the cell, or characterize new proteins byimmunoprecipitation. 1-3 pCMV-Tag1 Vector for Epitope Tagging Stratagenes pCMV-Tag1 vec...Antibodies for Studying NMDA Receptor Protein Expression and Synapse-Specific Immunolabeling
...dying expression of the NR2A, NR2B and NR2Csubunit proteins in different cell types and brain regions. These a... is known about theexpression of the NMDAR subunit proteins in situ. NMDA Antibodies Figure 1 Stratagene introduces Anti-NMDAR2A, Ant...High-Level Expression of Peanut Allergens Affected by Rare Codon Usage
Produce large quantities of difficult-to-express proteins Tamara Kleber-Janke Wolf-Meinhard Becker Bioche...sible to attain high-level expression of difficult proteins affected bypoor codon usage: IPTG-induced expression of several recombinant peanut allergengenes, su...Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells
... interest. Therefore, the induction of FLAG fusion proteins can bemonitored by Western blotting or other immunodetection methods using the a -FLAGM2 antibody (data not shown). Conclusions The Complete Control system has several advantages over other induciblesystems. The inducer exhibits no pleiotropic eff...Innovative Tissue Array Technology for High-Throughput Screening of Gene Expression
...tegrity of tissues and the antigenicity of proteins by assaying the Complete View array slides in hema... to the Complete View slides retainantigenicity of proteins and reactivity of nucleic acids, respectively. We areexpanding this product line to include human fe...Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
...It can beused to monitor expression of FLAG-tagged proteins in either prokaryotic oreukaryotic cells, to follow FLAG-tagged proteins during purifica- tion steps,and to identify proteins following immunoprecipitation. Furthermore, it ...Epitope-Tagging Vectors for Functional Analysis in Yeast
...igned to express high levels of eukaryotic proteins in S. cerevisiae. They are derived from the pRS400 series vectors. 2 Each pESC vector contains the following sequences: a ColE1ori-amp R fragment, which allows antibiotic selection and replication of the vector in E. coli fo...Safe Production of High-Titer Retrovirus
...id, ensures that expression of the viral packaging proteins is comaintainedwith the antibiotic-resistant genes...gag-pol fusion protein and each of the envelope proteins are expressed at sufficiently high levels to allow the production of viral supernatants with tite...... for monitoring gene expression and localizing proteins in vivo New Mammalian Expression Vectors E...nd high-level fluorescence of hrGFP fusion proteins within the cell. In addition, each of the four subcellular localization vectors shown in Fig...New Yeast Cloning System for Producing Proteins with Native Amino Acid Sequences
...ures rapid and high-yieldproduction of recombinant proteins in yeast. This system usesligation-independent clo...d and high-yield production of recombinant proteins in a eukaryotic organism. Because the system uses the yeast Schizosaccharomyces pombe as ...Optimizing Transfection Conditions for Studying Signal Transduction Pathways
...rplasmids express pathway-specific trans-activator proteins that consistof the yeast GAL4 DNA binding domain and the activation domains of varioustranscription factors. When a fusion trans-activator plasmid, reporterplasmid, and uncharacterized gene are cotransfected into mammalian cells, theuncharacterized g...Production of Full-Length, Biologically Active MEK1WT and MEK1CA
...sferase moiety. In E.coli, the improper folding of proteins is one of the most common causes ofinclusion body ...ing the problem of degradation is to express these proteins in eukaryoticcells, such as insect cells or yeast. In this article, we describe theexpression of MEK...New Protocols for Isolating High- Molecular-Weight Genomic DNA
...ree-floating RecoverEase kit cup wherethe digested proteins and low molecular weight contaminants are removed bydialysis (16 to 48 hours), leaving behind fully hydrated DNA, which is ready forimmediate use. From previous experiments using field inversion analysis, DNAisolated in this way, produced products th...Functional Cloning Using ViraPort Retroviral cDNA Expression Libraries
...tion/Screen Cell-surface proteins FACS sorting ...tact-inhibition Cell-cycle proteins Loss of contact-inhibition Phenotypic complementatio...RT-PCR Primer Sets for Human and Mouse Mismatch Repair Genes
...Cells cope with mismatches by enlistingspecialized proteins that can recognize, excise and correct mismatched ... binds to theDNA-bound, hMSH2-hGTBP complex. Other proteins are then recruited to completethe processes of incision and repair. The human hPMS2 protein performs...High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli
...tists who wish to use calmodulin affinity-purified proteins to studyprotein-protein interactions by pull-down ... amino acid sequence. Purification of recombinant proteins has been greatly simplified in recentyears due to the availability of expression vectors that allow ...Stable HeLa Luciferase Reporter Cell Lines Expressing GAL4 Fusion Transactivators
...id. When activatedby phosphorylation, these fusion proteins bind to the promoter and induceluciferase expression. Therefore, luciferase activity in stable cell linesreflects the activation status of the fusion transactivator and, hence, theactivation status of corresponding signaling pathways. The effects ofe...Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells
... in the transient transfection of greenfluorescent proteins into mammalian cells. We have been using the pEGFP...ous subcellular localization of some of our fusion proteins inthe same set of transfected cells. Presumably, this is due to varying levels ofexpression of exoge...Yeast Protein Production System Features High Yields and One-Step Purification
... many eukaryoticposttranslational modifications of proteins that can be critical for thebiological activity of...d identified, the demand forhomogeneously purified proteins for structural and functional analyses hasincreased. Although this demand can be partially fulfilled...Enzyme Immunoassay for Studying Intracellular Levels of cAMP
...ges in cAMP levels can result in the activation of proteins known to play key roles in signal transduction cascades, quantitative measurment of cAMP can serve to identify signaling events. Popular methods for determining cAMP levels include radioimmunoassays (RIA). Although the...Efficient Cleavage of Fusion Proteins to Yield Native Amino Termini
...r highly specific digestion of purifiedproteins or proteins in crude extracts Ed Marsh * Johanna Claycomb * ...minus of its recognition target.Cleavage of fusion proteins that contain the enterokinase (EK) recognitionsequence positioned between an Nterminal purification ...Expression and Purification of Recombinant Proteins That Have Native Amino Acid Sequence
...e inserted polypeptidesequence, cleavage of fusion proteins produced in the pCAL-n-EK vector yieldsthe desired...erminus of the polypeptide ofinterest and produces proteins with native amino acid sequence. 3 Protein coding sequences are cloned into the pCAL-n-EK vector by ...Detect Released Oligosaccharides Using the Eagle Eye II Still Video Imaging System
...ed against human P-glycoprotein in our lab. Sample proteins weretreated with PNGase F (Bio-Rad kit) to release all N-linkedoligosaccharides, following the kits instructions. All glycans were labeledwith ANTS using reductive amination (Bio-Rad kit procedure). The FACE/ANTS gelfluorophore-tagging method is prev...pFB-ERV: Retroviral Delivery of the Ecdysone Receptor Proteins
...viral vector for delivery of the ecdysone receptor proteins RXR and VgEcR.In stable cell lines infected with a...viral vector for delivery of the ecdysone receptor proteins RXR and VgEcR. Vector Description Fig.1 The vector pFB-ERV contains a tricistroni...Codon Bias-Adjusted BL21 Derivatives for Protein Expression
High-level expression of difficult heterologous proteins Carsten-Peter Carstens Anna Waesche Stratagene ... modification allows for a high-levelexpression of proteins that are difficult to express in conventional E. colihosts due to the codon bias of the gene of inte...Highly Active and Removable AffinityTagged Lambda Phosphatase
...phosphatase 4 that isuseful for dephosphorylating proteins phosphorylated on serine, threonine ortyrosine res...ed Lambda Phosphatase. Because many of the proteins that are phosphorylated in cells are kinases, the effect of phosphorylation on their own kin...New BL21-CodonPlus Cells Correct Codon Bias in GC-Rich Genomes
High-level expression of heterologous proteins Carsten-Peter Carstens Julie Bonnardel Anna Wa...e many innovations, the expression of heterologous proteins in E.coli can be difficult. Most frequently, failed or insufficient expression ofthe heterologous ge...hNT BlueNeurons Express b-Galactosidase at High Levels
...change the metabolism of amyloid precursor proteins and become permissive for infection by the human immunodeficiency virus (HIV). An increasing number of reports describe transplantation of hNT neurons intorodent brains.15 hNT neurons successfully integrate into surrounding tissue andestabli...High-Titer Retroviral Vectors for Gene Delivery
...tween the LTRs 6,7 is avoided by coexpressing both proteins from a single transcript. In light of published reports showing that a significant increase in titer isachieved by minimizing the amount of extraneous sequence between the LTRs, 3,7 we also constructed the vector pFB, which contains only the sequenc...TKB1 Cells Identify Receptor Tyrosine Kinase Interacting Proteins
...y, McMaster University,Ontario, Canada GST-fusion proteins expressed in TKB1 bacteria are used to affinity pu...lated, with the predominatetyrosine-phosphorylated proteins being the RTKs themselves. RTK tyrosinephosphorylation primarily functions to generate binding sites...In Vivo Signal Transduction Pathway Reporting Systems
...protein kinases, phosphatases and other regulatory proteins have been cloned,and many of these gene products a...el B), and plasmids that contain genes that encode proteins known to activate the trans-activator protein as a positive control ( figure 1 , pan...Improved Vectors for PathDetect Trans-Reporting Systems
...egalovirus (CMV) promoter for expression of fusion proteins ina wide variety of cell lines and the neomycin-re...r sequence and subsequentexpression of GAL4 fusion proteins in a variety of mammalian cells. The pFA-CMVvector features a multiple cloning site with 10 unique, ...RT-PCR Primers for the Study of Apoptosis
...nse. These primers are specific for genes encoding proteins fromthe Fas and Fas ligand, Bcl-2 and ICE protein ...edaccording to their functions in apoptosis. These proteins are Fas and Fasligands, Bcl-2 (and analogs), and the ICE families. The activation of death receptor...Keys to Successful Densitometry
... information from stained nucleic acids or proteins in gels. These functions provide a tool for quickly evaluating experimental data moments after an image has been acquired. To achieve more precise integrated density and concentration determinations, researchers using Eagle Si...A Comparison Study of Lipid Tranfection Reagents in A549, NIH 3T3 and COS-7 Cell Lines
... of lipid, we compared the concentration of cell proteins after transfections (data not shown). The A549 cell line seemed extremely tolerant to LipoTAXI transfection reagent as determined by the measurement of the total protein for the various volumes used. Even for 20 l of LipoTAXI ...Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
...ell line, we measured theconcentration of cellular proteins (data not shown). LipoTAXI reagent gave thehighest transfection efficiency and lowest toxicity in all three cell lines. Transfection Efficiencies: Effect of Mycoplasma Contamination When standardization studies are performed to determine optimized ...Mammalian and Bacterial Expression in One Vector
... was developed to allow simultaneous expression of proteins in prokaryotic and mammalian systems. The vector contains a hybrid bacterial promoter for inducible bacterial expression, the CMV promoter and enhancer region for constitutive mammalian expression and tandem consensus sequences for optimal translatio...Low-Toxicity, Lipid-Mediated Transfection of Mammalian Cells
...ignal intensity of a reportergene and the cellular proteins across a matrix of conditions, researchers caneasily optimize the amounts of DNA and LipoTAXI transfection reagent for anycell type. Optimizing Transfection Conditions The control plasmid provided with LipoTAXI transfection reagent encodes thebeta-...New Reporter Plasmids for Studying Interferon-Stimulated Signal Transduction Pathways
...on of interferons. Interferons (IFNs) are soluble proteins that act as local hormones, inhibitviral replicati...e certain host defense responses. Theseinteresting proteins modulate the expression of many genes and elicit a widerange of biological functions, including tiss...Eagle Eye Optical Filter Optimized for Imaging Silver-Stained Gels
...nt bands were more easily visualized. For example, proteins migrating at about 40 kDa are clearly visible in lanes 4 and 5 (earlier-eluting column fractions) when the silver-stain filter was used but do not become visible until lanes 7 and 8 (later-eluting fractions) when the ethidium ...Sequence-Validated and Expression-Tested Human cDNA in a Dual Expression Vector
...o be expressed and detected in mammalian cells and proteins to beexpressed in and purified from bacterial cell...pression vector. Potential uses for theseexpressed proteins include analyzing protein function, defining bothprotein-protein and protein-DNA interactions, eluci...New Fusion Trans-Activator Plasmids for Studying Signal Transduction Pathways
...id that expresses pathway-specific trans-activator proteins fused tothe yeast GAL4 DNA binding domain. To faci...s express pathway-specific trans-activator proteins that consist of the yeast GAL4 DNA binding domain and the activation domains of various ...Cis-Reporting System Monitors Intracellular Calcium Mobilization
...b), NFAT3, and NFAT4 (4a, 4b, 4c, and NFATx). NFAT proteins vary in sizefrom NFAT4a (708 amino acid residues) ...gh the dbd sequences of different isoforms of NFAT proteins have distinctaffinities, they bind to a consensus sequence of T(A)GGAAAAT(A/C). 1,2 This site is pre...Cis-Reporting System Monitors Intracellular Calcium Mobilization
...b), NFAT3, and NFAT4 (4a, 4b, 4c, and NFATx). NFAT proteins vary in sizefrom NFAT4a (708 amino acid residues) ...gh the dbd sequences of different isoforms of NFAT proteins have distinctaffinities, they bind to a consensus sequence of T(A)GGAAAAT(A/C). 1,2 This site is pre......e quantitative analysis of nucleic acids and proteins has established itself as a routine method in many...used to estimate the purity of nucleic acid, since proteins absorb at 280 nm. Pure DNA should have a ratio of approximately 1.8, whereas pure RNA sh...Sample Preparation for Microinjection
... labeled cells are sufficient to analyze labeled proteins by gel electrophoresis. ... result in the highest activity of the particular proteins or antibodies should be used. For peptide antibodies raised in rabbits, affinity ...... are ideal for the injection of DNA, RNA, proteins and dyes into living cells. The shapes of the capillary tip ensures high cell viability and minimizes blockages by the injection material. The capillary opening is constant, allowing reproducible injection volumes ...A Unique Dimension in Biological Purity
...n process. Contamination with nucleic acids, proteins or microorganisms by human contact or through other sources is excluded. Eppendorf certifies the purity of all Biopur products! This certificate* lists the limits for sterility, pyrogens (endotoxins), DNA, RNase and ATP. ......l, or the location of two different fusion proteins in one cell. Other variations of GFP are particularly designed for expression in mammalian cells or have up to a 35-fold higher fluorescence. With the aid of a GFP system with a drastically reduced half-life, dynamic processes......l, or the location of two different fusion proteins in one cell. Other variations of GFP are particularly designed for expression in mammalian cells or have up to a 35-fold higher fluorescence. With the aid of a GFP system with a drastically reduced half-life, dynamic processes...Decontamination and inhibition of ribonucleases
...nreacted DEPC which can otherwise react with other proteins and the adenine residues of RNA). As DEPC will attack polycarbonate (e.g. centrifuge tubes) or polystyrene (e.g. standard microtiter plates) decontamination of these materials can be achieved by soaking in 3% hydrogen peroxide for ...Isolation of Cosmids using Eppendorf's Perfectprep Plasmid Mini Kit
...NA is contaminated with impurities such as proteins or phenols. No inhibition or partial of digestion can be seen with the cosmids isolated with the Perfectprep Mini Kit. Additionally, DNA isolated with the Perfectprep Mini Kit can be used for other downstream applications with...Easy preparation of total RNA from cultured cells with TRIzol and Eppendorf Phase Lock Gel
...he RNA is of high purity and not contaminated with proteins or organic solvents measured by UV-absorption. Total RNA prepared with Eppendorf Phase Lock Gel can be directly used for downstream applications sensitive to any kind of impurities in the preparation. Reverse transcription of ...Efficient Recovery of Ultrapure Plasmid DNA
...n-column matrix, whereas polysaccharides and proteins show little or no affinity to the matrix at these salt concentrations. After only two washing steps, the pure plasmid DNA can thus be eluted from the silica matrix under low-salt conditions.2 With the Miniprep kit, the need for addi...HotMasterAn innovative Hot Start/Cold Stop technology for better PCR* results
...ot contaminate PCR reactions with unwanted proteins as do the Hot Start DNA polymerases that employ antibodies to achieve inhibition. Testing has also shown that HotMaster Taq can be used in real time PCR applications (data not shown). *This product is sold under licen...Other ContentsmicrosporangiummicrotubulesmicrotubulesmicrotubulesmidbrainmidbrainmigrationmigrationmigrationmigrationmigrationmigrationmigrationmineralsmineralsmineralsmineralsmineralsminisatelliteOther TagsDiesDiesDiesDiesDiesDiesAbroad
(Date:10/15/2008)...(1) published in the November issue of a scientifi...ished by Oxford Journals on behalf of the Society ...lamine and cyanuric acid based on research that wa...ds that contaminated pet food in North America in ...ween the pet food contamination that occurred in N...
(Date:10/15/2008)...) at the University of California, San Diego today...Triton Resource, a high-impact, massive data analy...tion, collaboration, and discovery through the use...DSC. , SDSC is leading the effort to build the T... Diego researchers. Specific details of Triton ar...
(Date:10/15/2008)...emain separate are crucial questions in evolutiona...rent animal species are often infertile or die whe...eproduce. A sheep-goat hybrid, the result of a cro...n. Such hybrids can also be dysfunctional, for exa...f sounds from both parent species. , Remarkably,...
(Date:10/14/2008)...n other planets and in extreme environments on Ear...ogy initiative under a five-year grant from NASA,s...om Earth and Beyond." , Christopher H. House, as...erdisciplinary team to develop methods to detect a...es in relevant ecosystems in ancient rocks and oth...
Breaking Biology News(10 mins):San Diego Supercomputer Center and UCSD announce 'Triton Resource' 2San Diego Supercomputer Center and UCSD announce 'Triton Resource' 3San Diego Supercomputer Center and UCSD announce 'Triton Resource' 4Turtle doves commit adultery 2Turtle doves commit adultery 3Penn State receives new NASA astrobiology grant 2Penn State receives new NASA astrobiology grant 3Diet and medications may assist prevention of prostate cancer 1789 1Diet and medications may assist prevention of prostate cancer 1789 2Study shows vitamin C is essential for plant growth 523 1Study shows vitamin C is essential for plant growth 523 2NEWSWEEK 3A Media Lead Sheet 3A October 1 2007 Issue 28on newsstands Monday Sept 24 29 1783 1NEWSWEEK 3A Media Lead Sheet 3A October 1 2007 Issue 28on newsstands Monday Sept 24 29 1783 2NEWSWEEK 3A Media Lead Sheet 3A October 1 2007 Issue 28on newsstands Monday Sept 24 29 1783 3NEWSWEEK 3A Media Lead Sheet 3A October 1 2007 Issue 28on newsstands Monday Sept 24 29 1783 4NEWSWEEK 3A Media Lead Sheet 3A October 1 2007 Issue 28on newsstands Monday Sept 24 29 1783 5Cataract Surgery 3A A Bargain Despite the Price 1780 1Cataract Surgery 3A A Bargain Despite the Price 1780 2Cataract Surgery 3A A Bargain Despite the Price 1780 3(Date:10/15/2008)... Will Educate Parents about Prescription,Drug Abus...mily Partnership (NFP),has designated Monday, Octo...g off the group,s 23rd annual Red Ribbon Week from...ents with action steps to prevent children,s,presc...million teens in the United States abuse,prescript...
(Date:10/14/2008)...4 /PRNewswire-USNewswire/ -- Kerby T.,Alvy, Ph.D.,...r for the,Improvement in North Hollywood (CICC), h... and Prevention (CDC) to serve as an expert to inf...or the prevention of child,maltreatment., CDC,s n...lationships,(SSNR) as the foundation for a unified...
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Breaking Medicine News(10 mins):Health News:National Family Partnership Designates October 27th 'Lock Your Meds' Day 2Health News:National Family Partnership Designates October 27th 'Lock Your Meds' Day 3Health News:Los Angeles Psychologist to Help CDC Fight Child Abuse 2Health News:HealthTalker Founder and CEO Andrew Levitt to be Featured at 'Health 2.0: User-Generated Healthcare 2008' 2[0] National Family Partnership Designates October 27th 'Lock Your Meds' Day[0] Los Angeles Psychologist to Help CDC Fight Child Abuse[0] HealthTalker Founder and CEO Andrew Levitt to be Featured at 'Health 2.0: User-Generated Healthcare 2008' [0] Warner Chilcott Announces Earnings Release Date and Conference Call for Third Quarter 2008 Financial Results
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