polyclonal

New Mammalian Two-Hybrid System Detects Protein-Protein Interactions

...body (Santa Cruz Biotech) and anti-NF- k B polyclonal antibody (Santa Cruz Biotech), respectively (data not shown). These results demonstrated that the mammalian two-hybrid assay kit detects known interaction between p53 and SV40 large T-antigen. In addition to the reporter plas...

Antibodies for Studying NMDA Receptor Protein Expression and Synapse-Specific Immunolabeling

...ene introduces an affinity-purified rabbit polyclonal synapsin antibody, Anti-Synapsin I, that can be used for studying synapsin expression in different cell types and brain regions. This antibody is suitable for studies of synapsin expression in a variety of different species, i...

Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells

...E, blotted, and probed with a -luciferase polyclonal antisera ( Figure 5C ). No significant detectable luciferase is expressed in the uninduced extract, whereas a linear increase in signal is observed from 300 nM to 5 M ponA. In a time-course experiment, a five-fold ind...

Detect Released Oligosaccharides Using the Eagle Eye II Still Video Imaging System

...nnheims DIG Glycan Differentiation kit. The rabbit polyclonal antibody(IgG) was raised against human P-glycoprotein in our lab. Sample proteins weretreated with PNGase F (Bio-Rad kit) to release all N-linkedoligosaccharides, following the kits instructions. All glycans were labeledwith ANTS using reductive amin...

Preparative Nondenaturing Gel Electrophoresis to Purify NADP-Specific Glutamate Dehydrogenase From Chlorella, Rev B

...column production, and the production of hightiter polyclonal and monoclonal antibodies. These results suggest that the Model 491 prep cell system is amenable to large-scale purification of partially purified proteins in their native, active form. The high resolution of this techn...

Purification and Characterization of beta-Lactoglobulin Genetic Variants A and B Using Preparative Electrophoresis and Isoelectric Focusing

...lectrophoresis, were also characterized by polyclonal antiserum to βLG (Nordic Immunologic, Tilburg...itrocellulose sheet was incubated with the polyclonal antiserum to βLG (5 l/50 ml PBS/0.05% Tween 20) for 2 h at RT, washed three times as ab...

Bio-Plex Cytokine Immunoassays and ELISA: Comparison of Two Methodologies in Testing Samples From Asthmatic and Healthy Children, Rev A

...ms (Abingdon, UK): capture antibody 2571.811 and a polyclonal detection antibody (goat anti-human). The detection antibodies were biotinylated and streptavidin-horseradish peroxidase was the enzyme label reagent. Results and Discussion Correlation between the Bio-Plex ...

Multiplex protein detection with the ECL Plex fluorescent Western blotting system using the Ettan DIGE Imager

... A3653 Rabbit polyclonal anti–human transferrin A0061 (Dako Cytomation) Monoclonal Anti–Actin, mouse-α-bovine A4700 (Sigma-Aldrich) Monoclonal Anti-&...

Mix-and-Read, Bead- and Cell-Based Immunoassays Using the FMAT 8100 HTS System

...nti-IL-8 antibody Dye A-labeled detection polyclonal anti-IL-8 antibody Dye B-labeled anti-ICAM-1, antibody (Figures 1 and 2) or CentriRed Stain (Figures 3 and 4) 5.Incubate overnight at room temperature and scan. Conclusion The increasing demands for hi...

Caspase-3 Activation - An Indicator of Apoptosis in Image-Based Assays

... incubated with cleaved caspase-3 (Asp 175) rabbit polyclonal antibody (Cell Signaling Technology, 9661, 1:100 dilution) in 5% goat serum and 0.3% Triton X-100 over night at 4C. Further wash steps removed unbound antibody prior to the addition of the secondary Alexa 488 goat anti-rabbit antibody (Invitrogen, A1...

DNA Damage and Repair - Quantification of Sub-Cellular Events Using Automated Confocal Imaging

...ed. 53BP1 was detected using the anti-53BP1 rabbit polyclonal antibody (Novus Biologicals, NB 100-304) diluted as described for H2AX. After 1h at room temperature, the primary antibody was removed and cells were incubated with Tween 20 (0.05% in PBS, 10 minutes). Two PBS washes prepared the cells for the...

High Sensitivity, Wide Dynamic Range, Horseradish Peroxidase (HRP) ELISA Using the LMax Microplate Luminometer (MaxLine Application Note #41)

...nd substances, horseradish peroxidase (HRP)-linked polyclonal antibody specific for TNF-α is added to the wells. Unbound antibody-enzyme reagent is removed by washing. An enhanced luminol/peroxide substrate solution is added to the wells to produce light. The reaction involves the oxidation of luminol to a...