polyadenylation

New Mammalian Two-Hybrid System Detects Protein-Protein Interactions

...essary for transcriptional termination and polyadenylation of the bait and target genes in mammalian cells. Stratagene offers this system as a vector kit containing both the baitvector, target (prey) vector, the pFR-luc reporter vector, and three controlvectors. All plasmid vectors are available separately....

Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System

... cloning site between the CMVpromoter and the SV40 polyadenylation signal. The pShuttle vector contains onlya multiple cloning site in which an entire expression cassette can be inserted.The regions indicated as arms are the stretches of sequence homology withpAdEasy-1 vector where homologous recombination occurs. T...

A New Lambda Vector for Mammalian Expression

... in a wide variety of cell lines; ### the SV40 polyadenylation site; and the neomycin-resistance gene under control of the prokaryotic b -lactamase promoter, to provide kanamycin resistance in bacteria, as well as the SV40 early promoter, to provide G418 resistance in mammalian c...

High-Titer Retroviral Vectors for Gene Delivery

... by the R region, which contains the viral polyadenylation signal (pA). The R region is followed by the U5 region, which, along with R, contains sequences that are critical for reverse transcription. Expression of the viral RNA is initiated within the U3 region of the 5 LTR ...

Mammalian and Bacterial Expression in One Vector

...ce of promoters, the presence of splicesignals and polyadenylation sites, as well as their respective translationinitiation sequences. In fact, the differences between bacterial and mammalianexpression vectors are so significant that, until now, these vectors could notbe used interchangeably. For expression i...

Mammalian Expression Vector for Efficient Cloning of PCR Fragments

...g features. The powerful CMV promoter and the SV40 polyadenylation site allow high-level constitutive expression in a wide variety of cell lines. In addition, the neomycin-resistance gene is under control of the prokaryotic b -lactamase promoter to provide kanamycin resistance in bac...

Cis-Reporting System Monitors Intracellular Calcium Mobilization

...ding sequence. 1,2,10,11 In addition to a polyadenylation signal downstream of the luciferase gene, the pNFAT-Luc plasmid contains another polyadenylation signal in front of the NFAT binding sites and TATA box. This design minimizes transcription ...

Cis-Reporting System Monitors Intracellular Calcium Mobilization

...ding sequence. 1,2,10,11 In addition to a polyadenylation signal downstream of the luciferase gene, the pNFAT-Luc plasmid contains another polyadenylation signal in front of the NFAT binding sites and TATA box. This design minimizes transcription ...

pSilencer 4.1-CMV: Versatile Vectors for Expression of siRNA, miRNA, and mRNA

... promoter) and an optimized SV40 polyadenylation signal to drive high-level expression of hairpin siRNA. For stable transfections requiring long-term antibiotic selection, an SV40 promoter expresses one of three antibiotic resistance genes (hygr...