Plasmid in Biological Technology

Senesco Signs a Supply Agreement for Polyplus-transfection's Delivery System

... therapy of siRNA against Factor 5A and a plasmid of the Factor 5A gene. Senesco has previously ... in vivo results using its combination siRNA and plasmid delivered with "in vivo-jetPEI" against ... delivery vehicle for a combination of siRNA and plasmid DNA in this therapeutic approach." About ...

Rentschler Biotechnologie Announces Cooperation with Boehringer Ingelheim

... proteins, protein scaffolds, monoclonal antibodies, antibody fragments and plasmid DNA. The Biberach site is specialized in highly efficient mammalian cell ... with exceptionally high productivities using proprietary systems. In the plasmid DNA manufacturing arena Boehringer Ingelheim in Austria has set the ...

Genetic Immunity To Present at the Rodman & Renshaw 5th Annual Global Healthcare Conference

... is a patented nanoformulation technology that includes disease-specific plasmid DNA encoded antigens. DermaPrep is the topical administration device that ... of diseases. These two components together make up the DermaVir Patch. The plasmid DNA within DermaVir Patch's NanoComp nanoparticles is specific to HIV and ...

EGEN, Inc. Announces the Promotion of Jason Fewell, Ph.D., to Vice-President of Preclinical Research and Development

... their novel compositions with nucleic acids and their therapeutic applications. EGEN's research pipeline includes products involving siRNA, shRNA and plasmid DNA for the treatment of various cancers and other diseases and has a number of collaborations with outside investigators, biotech organizations and ...

Frost & Sullivan Recognizes Cisbio Bioassays for Technology Innovation

... of one successful reaction during which it covalently links to its substrate. These substrates can be derivatized with HTRF fluorophores. A plasmid construct can be easily generated where the SNAP or CLIP tag is attached to the N-terminus of the GPCR. Thus, a fusion protein comprised of the ...

EGEN, Inc. Announces the Promotion of Khursheed Anwer, Ph.D., to President and Chief Scientific Officer

... their novel compositions with nucleic acids and their therapeutic applications. EGEN's research pipeline includes products involving siRNA, shRNA and plasmid DNA for the treatment of various cancers and other diseases and has a number of collaborations with outside investigators, biotech organizations and ...

Clontech Laboratories, Inc. Releases the Xfect(TM) and Xfect(TM) Stem Transfection Reagents

... transfection reagents. These novel transfection reagents are based on biodegradable nanoparticles that permit superior transfection efficiency of plasmid DNA into mammalian cells. For added ease of use, transfections can be carried out entirely in the presence of serum. Xfect shows high efficiency over ...

ImmunoVaccine Technologies Partners with FIT Biotech to advance a therapeutic HIV vaccine

... with FIT Biotech, a Finland-based, clinical stage company that develops DNA vaccines. This research will formulate FIT Biotech's GTU(R) MultiHIV DNA plasmid with IVT's DepoVax(TM) vaccine delivery system to advance a therapeutic HIV vaccine. "DepoVax(TM) will act as a vector to deliver FIT Biotech's ...

CBDM.T, The Market and Business Intelligence Company Reviews The Vaccine Market

... or attenuated organisms, pathogen protein toxin (toxoids), pathogen surface molecules, inactivated or attenuated virus and more recently from DNA plasmid carrying an antigen-coding gene. According to CBDM.T, vaccines represent one of the fastest growing pharmaceutical product categories at ...

GeoVax Labs, Inc. Reports Fourth Quarter and Year-End 2008 Financial Results

... MVA viral vaccine. * In December 2008, GeoVax engaged the services of VGX International, Inc. (VGXI), whereby VGXI will manufacture plasmid (DNA) for use in GeoVax's future preventative and therapeutic clinical human trials. Strengthened Management Team * In February ...

Senesco Technologies Reports Second Quarter Fiscal 2009 Financial Results

... "Preclinical Studies Using Polyethylenimine (PEI) Nanoparticles Complexed with Eukaryotic Translation Initiation Factor 5A (eIF5A) siRNA and eIF5A plasmid DNA Demonstrates Significant Anti-Myeloma Activity in Vitro and in Vivo". Senesco's preclinical multiple myeloma data was presented at the ...

Sangamo BioSciences Announces Results From Diabetic Neuropathy Clinical Trial SB-509-601

... and 719-457-0820, respectively. The conference ID number for the replay is 2585543. About SB-509 SB-509 is an injectable formulation of a plasmid encoding a zinc finger DNA-binding protein transcription factor (ZFP TF(TM)) designed to upregulate the expression of the gene encoding vascular ...

Sangamo BioSciences Reports Third Quarter 2008 Financial Results

... velocity (NCV) in 75% of SB-509 treated subjects compared to 25% of placebo treated subjects. SB-509 is an injectable formulation of plasmid DNA that encodes a ZFP transcription factor (ZFP TF(TM)), designed to upregulate the vascular endothelial growth factor-A (VEGF-A) gene. * ...

Expression Genetics' EGEN-001 Named One of 10 Most Promising Oncology Products by Windhover

... solid tumors. The product is based on proprietary TheraPlas(R) delivery technology. It is composed of an interleukin-12 (IL-12) gene expression plasmid and a biocompatible delivery polymer. IL-12 is a potent cytokine which works by multiple mechanisms of action to enhance the body's immune system ...

Sangamo BioSciences Reports Second Quarter 2008 Financial Results

... with mild to moderate diabetic neuropathy over a six month period after a single administration of SB-509. SB-509 is an injectable formulation of plasmid DNA that encodes a ZFP transcription factor (ZFP TF(TM)), designed to upregulate the vascular endothelial growth factor-A (VEGF-A) gene. -- Phase 2 ...

Sigma-Aldrich Joins Phase 2 of The RNAi Consortium to Validate shRNA Libraries

... mouse genomes. Sigma-Aldrich's MISSION(R) shRNA portfolio is the exclusive source for researchers to access TRC and TRCII shRNA libraries in the plasmid DNA and lentiviral formats. All of the shRNA reagents will be fully functionally validated during phase 2 of The RNAi Consortium. This validation ...

On the trail of rogue genetically modified pathogens

... the artificial vector sequences into different subgroups based on shared sequence; these shared sequences were then compared with the natural plasmid and chromosomal sequence information so as to find regions that are unique to the artificial vectors. Nearly all the artificial vector sequences had ...

Altogen Biosystems Launches Custom Biotechnology Research Services: Generation of Stable Cell Lines and RNAi Cell Transfection Services

... ) include: -- Transfection services (transient and stable) -- Assay development and library screening -- Cloning and plasmid vector construction -- Generation of stable cell lines -- RNAi experiments (siRNA design, synthesis, validation) -- Gene targeting (mRNA ...

Genetic Immunity Announces the Appointment of Richard B. Pollard, M.D. as Chief Medical Officer

... trials, is comprised of two patented components: NanoComp and DermaPrep. NanoComp is an antigen composition platform that combines a disease-specific plasmid DNA with the Company's formulation technology. The unique DNA for the DermaVir Patch product encodes the majority of HIV antigens, allowing a broad ...

Sangamo BioSciences Announces ZFP Therapeutic Data From Nerve Regeneration Program at Society for Neuroscience Meeting

... who is world-renown for his work on SCI and has significant clinical trial experience in this area." Sangamo is currently developing SB-509, a plasmid formulation of this same ZFP TF, for the treatment of diabetic neuropathy and has two ongoing Phase 2 trials in this area. In addition, the Company ...

QuickLyse Miniprep Kit

... For ultra-fast, sequencing grade plasmid DNA minipreps The QIAGEN ... ultra-fast preparation of up to 15 g plasmid DNA. The quality of the DNA ensures ... Reproducible yields sufficient plasmid DNA for any downstream application (Figure ...

A simple method to sequence from bacterial colonies using [a-33P] radiolabeled ddNTPs and Thermo Sequenase

... Such high sensitivity allows sequencing of plasmid DNA directly from bacterial colonies with minimal ... kit, offers an excellent method to sequence plasmid DNA directly from colonies. Sequence information ... the size of the colony is smaller than 2mm or if plasmid is present in low copies, increasing the number ...

Aurum Plasmid 96 Kit Tech Note, Rev A

... E. coli bacteria have long been used as hosts for plasmid propagation. Several methods have been ... sodium iodide and sodium perchlorate, plasmid purification using glass powders and diatomaceous ... Yang et al. 1979, Marko et al. 1982). Typically, plasmid purification kits use alkaline lysis followed ...

Transient Gene Expression Following Single and Double Exponential Decay Electroporation Pulses

... principal finding was that cotransfection of a plasmid encoding the SV40 large T antigen with a reporter plasmid bearing the SV40 origin of replication ... are an accurate representation of the plasmid uptake and gene expression in these cell samples. ...

Transgenic Zebrafish by Electroporation

... To establish our technique we used the plasmid construct RSVCAT (Gorman et al., Proc. Natl. ... Sci. USA, 79 , 6777-6781, 1982). This plasmid uses the Rous sarcoma virus (RSV) promoter ... fresh water. plasmid Preparation ...

Transformation of Filamentous Fungi by High-Voltage Electroporation

... of Trichoderma harzianum with plasmid DNA. Materials and Methods ... used as the transformation host. The plasmid used was pAN7-1, a derivative of pUC19 containing ... nidulans (Punt et al., 1987). plasmid DNA was purified from E. coli MC1061 ...

Electroporation of Primary Bone Marrow Cells

... either a murine MHC class II gene, A B b , or a plasmid encoding a mutant dihydrofolate ... 10 days in culture. Cells receiving the dhfr plasmid were cultured with increasing amounts ... hybridization of the dhfr-containing plasmid to sequences within the test cells, yet no ...

Transformation of Filamentous Fungi by Microprojectile Bombardment

... Materials and Methods Strain and plasmid Aspergillus nidulans strain A773 ... microprojectile bombardment. plasmid pRG-1, a 5 kb plasmid containing the Neurospora crassa pyr4 gene ...

Sub-Micron Gold Particles Are Superior to Larger Particles for Efficient Biolistic Transformation of Organelles and Some Cells

... with the PDS-1000/He instrument using plasmid DNA coated onto gold or tungsten ... with pBC17 (Goff, et al., 1990), a plasmid which encodes genes for the C1 ... and all particles were coated with 0.33 g of plasmid pBC17; 16 target tissue pieces were ...

Single-Cell Complementation of Barley Mlo Mutants Using a PDS-1000/He Hepta System

... efficiencies were obtained upon delivery of a plasmid carrying both Green Fluorescent ... et al. 1999). Plasmids plasmid pUGLUM (Zhou et al. 2001) carrying GFP and Mlo ... unpublished) was used. In this plasmid the β-glucuronidase reporter gene is driven ...

Optimization of Biolistic Transformation Using the Helium-Driven PDS-1000/He System

... 1, footnote c). Plasmids plasmid YEp352 (Hill, et al., 1986), which contains a ... tissue was bombarded with the plasmid pBI221, which contains the GUS gene expressed ... prepared, and coated with supercoiled plasmid essentially as described by Sanford, et al. ...

Detection of Reporter Gene Activity in Cell Cultures and Murine Epidermis After Helios Gene Gun-Mediated Particle Bombardment

... and conditions used for plasmid inoculations. Expression from these plasmids ... this study. PLASMIDS The plasmid pCMV--gal (Clontech, Palo Alto, CA) was used in ... under in vitro and in vivo conditions. The plasmid pCMV without the -galactosidase gene ...

Quantitation of Lymphangiogenesis Using the iCycler iQ Real-Time PCR Detection System and Scorpions Detection System, Rev A

... for cloning. The agarose gel extraction kit and plasmid extraction kit were purchased from ... of the product. LYVE-1 Cloning and plasmid Preparation The LYVE-1 PCR product ... vector and One Shot E. coli, and the plasmid extracted from the E. coli using ...

Identification of Nonspecific Products Using Melt-Curve Analysis on the iCycler iQ Detection System, Rev A

... standard curves with the IL-1β plasmid template (IMAGE Consortium clone 324655) using ... of consecutive 5-fold dilutions of the IL-1β plasmid in ddH 2 O was made, creating five ... tubes. Then a 6 l aliquot from each plasmid dilution was added to one of the tubes ...

Real-Time Multiplex PCR from Genomic DNA Using the iCycler iQ Detection System

... DABCYL-3', Operon) 1.0 l plasmid DNA dilution 20.2 l ... we demonstrate here on genomic and plasmid targets. Furthermore, we demonstrate the need ... conditions. This was true for plasmid or genomic DNA targets. Figure 1 (left-hand ...

Quantitation of DNA for Automated Sequencing Using the VersaFluor Fluorometer

... SK+ (Stratagene) and transformed into DH5a cells. plasmid DNA was isolated using anion exchange ... screen of a tadpole library. Rescued plasmid DNA was obtained using the manufacturers ... subsequently transformed into DH5α cells. plasmid DNA was isolated using anion exchange ...

Qualitative and Quantitative Assessment of Plasmid DNA Isolation, Rev A

... Small-scale isolation and purification of plasmid DNA from E. coli bacterial strains is ... research, it is vitally important that plasmid DNA isolation and purification protocols and ... new Aurum miniprep and midiprep plasmid DNA isolation kits. We conclude that ...

High Electrotransformation Efficiencies Obtained With DNA From Ligation Mixtures

... cDNA libraries from a small amount of plasmid DNA necessitates the high transformation ... E. coli JS5 cells and supercoiled pUC18 plasmid DNA (10 pg/l) were obtained from Bio-Rad Laboratories. Linear pUC18 plasmid was prepared by digestion of the ...

Electrotransformation of E. coli With M13 DNA

... This inhibition is not observed with plasmid or conventional transformations. The problem was ... the mixture was plated as above. For plasmid DNA transformation, SOC medium (SOB ... a significantly reduced efficiency from that of plasmid DNA (Table 2, samples 1 and 2) which ...