Phage in Biological Technology

Tips from the Journals of the American Society for Microbiology

... phage Therapy May Control Staph Infections in Humans ... higher mortality rate. A bacteriophage, or phage for short, is a virus that infects bacteria. In ... These results suggest a potential use of the phage for the control of both local and systemic human ...

Calvert Research and Auburn University Announce Joint Venture

... pharmaceutical nanocarriers, such as liposomes, with breast tumor-specific phage proteins may significantly enhance their anti-cancer activity. "This ... Petrenko said. "Their experts first recognized that the merging of phage nanobiotechnology developed at Auburn with genomics can contribute ...

Peregrine Pharmaceuticals Licenses Anti-VEGF Antibodies to Affitech

... clinical data." Commenting on the licensing agreement, Dr. Achim Kaufhold, chief executive officer of Affitech, said, "Using advanced phage display technologies, Affitech successfully identified fully human, potent and selective anti-VEGF antibodies as part of a previous research and ...

TechConnect World Publishes Conference Agenda Featuring More than 2,700 Presentations Addressing Nano, Bio and Clean Technologies

... Medical & Biotech Track - focusing on cancer research; drug delivery; nano medicine; Bio-nano materials; biosensors & diagnostics; phage engineering, and other topics. Business & Strategy Track - addressing novel products, investments & business; education & ...

Frost & Sullivan Recognizes ESBATech's Innovation in Developing Antibody Fragments for Biologic Therapeutics

... independent screening platform, Immuna(R), for selecting and optimizing highly-stable, single-chain antibody fragments (scFv) without any phage display." ESBATech's successful screening and characterization of all 1.5 million naturally occurring antibody fragment frameworks resulted in ...

Using living cells as nanotechnology factories

... methods to copy branched DNA structures, and there has also been significant work in using long-stranded DNA sequences replicated from cells or phage viruses to scaffold short helper DNA sequences to form 2-D or 3-D objects," said Yan, who is also a professor in the Department of Chemistry and ...

Strategic Diagnostics Reports Fourth Quarter and Full-Year Financial Results

... producing plants for further testing. The Company's joint development program with POET Energies has been suspended. "We remain excited about our phage technology and confident it can bring significant value to the industrial bio-fermentation industry," Mr. Knight commented. "We are considering ...

Nastech Assigns Intellectual Property Related to the Development of RNA-Based Therapeutics to its Subsidiary, MDRNA, Inc.

... siRNA Duplex -- RNAi Therapeutic for Respiratory Virus Infection -- Compositions and Methods for Complexes of Nucleic Acids and Peptides -- phage Displayed Cell Binding Peptides -- Dry Powder Compositions for RNA Influenza Therapeutics -- Nanoparticles for Delivery of Nucleic Acids and ...

Efficient Cloning and Electro-transformation of Large Eukaryotic DNA Fragments

... constrained to 4045 kb by the packaging limit of phage lambda. Recently, three systems have ... (YAC)-based vectors (>500 kb limit), 1 phage P1-based vectors (100 kb limit), 2 ... C., Vieira, J., and Messing, J., Improved M13 phage cloning vectors and host strains: ...

Cloning Based on Efficient Three-Fragment Assembly DNA Ligation

... To construct a phage displaying integrin-binding ligands, we needed ... ligation reactions, with a molar ratio of phage DNA to insert A and insert B of 1:5:5. Standard ... from incompletely cleaved and self-ligated phage DNA backbone. A relatively small proportion of ...

M13/Phagemid DNA Extraction Protocol

... of PLG. (3) Propagate M13 phage by one of the standard procedures: ... more than 3 days) with 300 l M13 phage stock (1 x 1011 pfu/ml) and incubate 5 minutes at room temperature to allow phage to interact with the ...

M13/Phagemid DNA Extraction Protocol

... of PLG. (3) Propagate M13 phage by one of the standard procedures: ... more than 3 days) with 300 l M13 phage stock (1 x 1011 pfu/ml) and incubate 5 minutes at room temperature to allow phage to interact with the ...

A New Lambda Vector for Mammalian Expression

... 3 x 10 6 primary plaques. The phage titer of the amplified library was 2 x ... suitable helper phage VCSM13, ExAssist helper phage R408, RE704, ...

Purified Single-Stranded M13 DNA for Automated Sequencing

... The yield of single-stranded DNA recovered from phage lysate exceeds the yield obtained with the ... polyethylene glycol (PEG)-NaCl precipitation of phage particles, phenol-chloroform extraction to remove phage coat proteins, and ethanol precipitation to ...

Inactivation of Contaminating Templates in PCR Using High Intensity Ultraviolet Light

... Amplification of DNA was performed using genomic DNA from lambda phage and purified amplified human β-actin DNA. All of the tubes used in the amplification of a 500 bp fragment of lambda phage contained 50 ng of total lambda genomic DNA suspended in 5 l of ...

Electrotransformation of E. coli With M13 DNA

... abundant, easily isolated single-stranded form of the mp series M13 phage DNA is a superior starting material for sequencing and in vitro ... LacZ fusion cloning scheme allows easy detection of recombinant phage over background. During the process of evaluating and ...

HotMasterAn innovative Hot Start/Cold Stop technology for better PCR* results

... the Eppendorf Mastercycler gradient using human genomic DNA or phage Lambda DNA (New England BioLabs, USA) as indicated. The ... Experiment 2: Processivity A 5.0 kb phage Lambda target was amplified using Eppendorf HotMaster Taq DNA ...

Lambda DNA Extraction Protocol

... MgSO4, pH 7.5) overnight at 37C. Dilute lambda phage stock to l05 pfu per 0.1 ml in SM buffer (0.1 M NaCI, 8 mM ... bacteria in a sterile 13 x 100 mm culture tube and allow phage to adsorb to bacteria for 20 minutes at 37C. ...

Lambda DNA Extraction Protocol

... MgSO4, pH 7.5) overnight at 37C. Dilute lambda phage stock to l05 pfu per 0.1 ml in SM buffer (0.1 M NaCI, 8 mM ... bacteria in a sterile 13 x 100 mm culture tube and allow phage to adsorb to bacteria for 20 minutes at 37C. ...

PCR Performance Comparisons Between pfuturbo and Taq DNA Polymerases

... target from rodent genomic DNA (lane 5) and a 14-kb target from lambda phage DNA (lane 6). In contrast, Taq DNA polymerase had difficulty amplifying ... using 200 M each dNTP, 100 to 300 ng of genomic DNA or 15 ng of lambda phage DNA, and 100 ng of each oligonucleotide primer per 50 l reaction. PCR ...

Novel PCR Enhancing Factor Improves Performance of Pfu DNA Polymerase

... of DNA targets, ranging in complexity from plasmid and lambda phage DNA templates to complex, single-copy amplicons from genomic ... For example, Pfu DNA polymerase amplified an 8-kb target from lambda phage DNA (lane 16), but not a 14-kb lambda DNA target (lane 20) nor a 10-kb, ...

A Positive Selection Assay for Mutation Analysis in Big Blue Animals

... functions as a mutational test sequence. Rescued shuttle vector phage containing lacI mutations are scored as blue plaques ... (37C overnight) to determine the total number of rescued phage screened ( figure 2 , path B). Because the l LIZ shuttle ...

Resolution of Linear DNA Fragments From 23 Kilobases to 6 Megabases Using Biphasic Linear Switch-Time Ramping

... of Schizosaccharomyces pombe (closed circles, lanes 4 and 6), Saccharomyces cerevisiae (open squares, lanes 3 and 5), lambda phage concatomers (open circles, lane 2), and the larger fragments of Hind III-digested lambda DNA (closed squares, lane 1) migrate as an ...

Quantitation of DNA for Automated Sequencing Using the VersaFluor Fluorometer

... by a homology screen of a Xenopus muscle genomic library using a 32 P-dCTP labeled mouse probe (a gift of Steven McKnight). phage DNA was isolated using a standard protocol, digested with EcoRI and HindIII and run on a 1.2% agarose gel (Figure 1). Bands ...

Efficient and Reliable PCR Setup Using Eppendorf MasterMix

... the coding sequence of the pyrophosphatase gene of Thermo thermophilus (70% GC content) and a 10-kb fragment from the genome of the phage lambda was amplified. The target DNA used was human genomic DNA, T.th genomic DNA or lambda DNA. The PCR reaction volumes were ...

Isolation of Cosmids using Eppendorf's Perfectprep Plasmid Mini Kit

... a capacity to hold fragments of DNA ranging from 30kb and 45kb DNA [1]. Cosmid structures were created from a hybrid of both phage and plasmid traits [2]. The intrinsic characteristics of cosmids allow them to undergo efficient ligation, transfection, replication and ...

High-efficiency Derivatives of the BL21 Series for Protein Expression

... ## These strains have been engineered either to contain an inducible T7 RNA polymerase gene or to readily allow one to be introduced by phage infection. 1 BL21 competent cells contain a mutation in the ompT protease gene, which is implicated in the degradation of some recombinant ...

Tissue Specificity for Mutation Parallels Tissue Specificity for Cancer

... rodent tissue, which can be facilitated with the RecoverEase DNA isolation kit, and the l genomes are packaged into phage particles by Transpack lambda packaging extract.** The l particles are then plated on a specially constructed E. coli host ...

Mammalian and Bacterial Expression in One Vector

... Figures 2 Inducible Expression of GFP-CBP Fusion Protein in BL21(DE3) Cells The hybrid T7/lacO phage promoter was tested by carrying out induction studies using IPTG. BL21(DE3) cells harboring the KPdual-1 construct were grown ...

Optimizing pfuturbo DNA Polymerase Amplification Reactions with Perfect Match PCR Enhancer

... genomic DNA and the 6.2- and 6.6-kb fragments were amplified from Big Blue transgenic mouse genomic DNA that contained 40 copies of lambda phage DNA per genome. PCR reactions were conducted in a Stratagene RoboCycler Gradient 96 temperature cycler fitted with a Hot Top Assembly, ...

Easily Amplify Genomic DNA with Long-Distance PCR

... MHC17 (20 pg per reaction) was used in parallel as a template for control amplifications ( Figure 2 , Panel A). This phage carries 19 kb of genomic DNA containing a portion of the mouse MHC gene ( Figure 1 ). In Figure ...

New Protocols for Isolating High- Molecular-Weight Genomic DNA

... subjected to in vitro packaging extracts, yielded large numbers of rescued shuttle vector. Table 1 Average Lambda phage Recovery with RecoverEase DNA Isolation Kit and Organic Extraction Methods Tissue ...