Paraffin in Biological Technology

Interim Report for the Period 1 January - 30 September 2008 (Unaudited)

... molecular diagnostic tests can be performed on tissue in formalin fixed paraffin embedded blocks (FFPE). Portfolio of cellular based Oncotech EDR ... procedures at hospitals as most tissues are available as formalin fixed paraffin embedded blocks (FFPE). Providing molecular diagnostic tests based on ...

HER1 and HER3 VeraTag(TM) Assays are Now Available for Use in Development and Clinical Evaluation of Cancer Therapeutics

... measurements of protein expression and activity in formalin fixed paraffin embedded (FFPE) tumor samples. These assays supplement HERmark(TM), ... the molecular level. VeraTag is designed to run on standard formalin-fixed paraffin embedded (FFPE) patient samples. About Monogram Monogram is advancing ...

Femtogram-level chemical measurements now possible, U. of I. team reports

... As a demonstration of the technique, the researchers scanned a piece of paraffin with their probe, and removed a sample for analysis. They then used Raman ... spectroscopy to chemically analyze the sample. After analysis, the paraffin was removed by thermal decomposition, allowing reuse of the probe. We ...

World BioBank(TM) Launch Benefits Advanced Medical Research

... the highest quality samples and data. Sample types routinely collected and housed include: solid tissues (snap frozen and matched formalin-fixed paraffin embedded diseased and normal internal controls) from surgical resections, image-guided biopsies, bone marrow biopsies and endoscopic biopsies. ...

Monogram Announces HERmark(TM) Oral Presentation at the 44th ASCO Annual Meeting

... or signaling pathway activity allowing for disease characterization at the molecular level. VeraTag is designed to run on standard formalin-fixed paraffin embedded (FFPE) patient samples. About Monogram Monogram is advancing individualized medicine by discovering, developing and marketing innovative ...

Biomatrica's PCRboost(TM) Enables 5-Fold Improvement in Amplification of Difficult Samples

... resulting in significant time and cost savings. "With PCRboost, I obtained at least a 5-fold increase in the amplification of formalin-fixed paraffin embedded (FFPE) cervical cancer tissue samples compared to using water in a standard master mix," said Mina Kalantari, Ph.D., University of ...

Henry Ford Hospital and Rosetta Genomics to Develop MicroRNA-based Diagnostics for Brain Cancer

... from this analysis, Rosetta Genomics will use its proprietary microRNA extraction technologies to perform a microRNA analysis from Formalin Fixed paraffin Embedded (FFPE) samples received from Henry Ford Hospital. "The goal of our collaborations with the leading cancer research centers is to seek new ...

Response Genetics' CEO to Discuss Advances in Optimizing Therapy through Pharmacogenomics as Part of a Series of Presentations at AACR-NCI-EORTC International Conference

... RGI's technologies enable extraction and analysis of genetic information from genes derived from tumor samples stored as formalin-fixed and paraffin embedded specimens. RGI currently generates revenue primarily from the sales of its proprietary analytical pharmacogenomic testing services of ...

Response Genetics Announces Presentations/Webcasts at Upcoming Investor Conferences

... RGI's technologies enable extraction and analysis of genetic information from genes derived from tumor samples stored as formalin-fixed and paraffin embedded specimens. RGI currently generates revenue primarily from the sales of its proprietary analytical pharmacogenomic testing services of ...

Response Genetics Reports Second Quarter 2007 Financial Results

... RGI's technologies enable extraction and analysis of genetic information from genes derived from tumor samples stored as formalin-fixed and paraffin embedded specimens. RGI currently generates revenue primarily from the sales of its proprietary analytical pharmacogenomic testing services of ...

A Novel Process for Gene Expression Profiling of Rat and Mouse Tissues from Formalin-Fixed Paraffin-Embedded Sections Using Microarrays

... half fixed in 10% neutral buffered formalin for paraffin sections (FFPE) and the other half embedded in ... were stored at 70C until use. 2.2 paraffin Sections: a. Tissues ... the tissues were immediately processed into paraffin blocks by taking them through a series of ...

Optimum FFPE RNA Isolation Kit

... Total RNA Isolation from Formalin Fixed paraffin Embedded (FFPE) Tissues ... or paraformaldehyde fixed paraffin embedded (FFPE) tissues that ... 3) Excessive Amount of paraffin Used for Embedding Tissues Two times ...

Microfluidic analysis of multiplex PCR products for the genotyping of Helicobacter pylori

... allelic variants from DNA extracted from paraffin wax embedded tissues. The use of template DNA derived from formalin fixed and paraffin wax embedded tissue for the mPCR restricts ... Tissues The paraffin embedded and formalin fixed gastric tissues ...

Detecting Gastrin-Releasing Peptide Receptor by in situ PCR on Archived Tissue

... (RTISPCR) is a recently described method for detecting mRNAs in archival paraffin tissue blocks. This method is readily performed using the RoboCycler 40 ... tract tumor. By detecting mRNAs in tissues that have been maintained in paraffin blocks and archived for over 50 years, this technology promises to ...

Innovative Tissue Array Technology for High-Throughput Screening of Gene Expression

... fetal validation and survey arrays. Methods In Situ Hybridization: paraffin was removed from the array tissue by treating the slides as described in ... with an Olympus BX60 fluorescent microscope. Immunohistochemistry: paraffin was removed from the array tissue by treating the slide as described in ...

Orbitec will use $25 million Air Force contract to make small launch vehicle

... liquid propane-oxygen vortex engines, with tests slated for two years or more from now. A separate project focuses on hybrid rocket engines that burn paraffin wax. Testing for "Forward One," an Air Force-funded project to develop a 7,000 to 10,000-pound thrust-vortex engine fueled by hydrocarbons and ...

Genomic Analysis of Formalin-Fixed Paraffin Embedded (FFPE) Tissues through the use of Whole Genome Amplification (WGA)

... and protocols. Org. Divers. Evol. 3 : 195-205 5. Legrand, B., et al., (2002) DNA genotyping of unbuffered formalin fixed paraffin embedded tissues . Forensic Sci. Int., 125 : 205-11. 6. Siwoski, A., et al., (2002) An Efficient Method for the Assessment of ...

A new wide field-of-view confocal imaging system and its applications in drug discovery and pathology

... technique enables researchers to extract small cylinders of tissue from histological blocks and arrange them in a matrix configuration in a recipient paraffin block, such that hundreds or even thousands can be analyzed simultaneously for morphological or molecular changes. 37, 38 The advantages of TMA ...

GenomiPhi amplification of FFPE tissue DNA for array CGH

...

5. Jones, K. E. et al . DNA Isolation and amplification from formalin fixed, paraffin embedded tissues. Presented at American Society for Human Genetics Annual Meeting, Toronto, Canada, 2004.

6. Cardoso, J. et al . ...

Working With Laser Capture Microdissection Samples

... discrete morphological structures from thin tissue sections. For LCM, frozen and OCT embedded, or formalin-fixed and paraffin embedded tissue sections are processed, stained, and dehydrated, then scanned under an inverted microscope. Cells are ...

Gene-Expression Analysis of TP, DPD, and TS Using the LightCycler mRNA Quantification KitsPLUS

... Total RNA was isolated from PET in 4 x 5-m slides and from matched fresh-frozen tissues in two 20-mg slices according to the High Pure RNA paraffin Kit protocol. As depicted in Figure 5, the relative expression rates of TP, DPD, and TS are comparable in both tissue types. ...