Nuclei in Biological Technology

Berkeley scientists bring MRI/NMR to microreactors

... made possible by a property found in the atomic nuclei of almost all molecules called spin, which gives rise to a magnetic moment, meaning the nuclei act as if they were bar magnets with a north and ... an MRI/NMR signal depends upon an excess of nuclei in a sample with spins pointing in one direction ...

Scientists determine the structure of highly efficient light-harvesting molecules in green bacteria

... exposed to a magnetic field -- to look deep inside the chlorosome. This technique enables researchers to understand the relationships between atomic nuclei in a sample and, ultimately, to acquire structural information about the molecules of interest. "The NMR data revealed to us that the individual ...

Animal Eggs Not Suitable Substitutes to Produce Stem Cells

... that focuses on understanding developmental plasticity and defining the molecular mechanisms that regulate differentiation or dedifferentiation of nuclei and cells. Tables of contents and a free sample issue may be viewed online at www.liebertpub.com/clo Mary Ann Liebert, Inc. , is a privately ...

Spallation Neutron Source sends first neutrons to 'Big Bang' beam line

... the universe," Greene said. "Another set of extremely precise studies at the FNPB will address the interaction between neutrons and simple nuclei and may help to explain universal 'parity' violation," Greene said. "Roughly speaking, parity is the symmetry that implies that the laws of physics ...

NIST/NIH micromagnets show promise as colorful 'smart tags' for magnetic resonance imaging

... properties than conventional injectable MRI contrast agents. MRI contrast agents enhance images by altering the magnetic field seen by hydrogen nuclei in water. Conventional contrast agents are chemically synthesized whereas the new micromagnets are microfabricated. This allows for greater control ...

Young's experiment in a hydrogen molecule

... properties. The authors of this current research, which appears in the latest issue of the journal Science, uses electrons instead of light and the nuclei of the hydrogen molecule as emitting slits. CSIC researcher Ricardo Dez, Vicedirector of the Centre for Materials Physics (a mixed body of the ...

Improving the accuracy and speed of mammalian cell counting

... (PI), which intercalates to DNA in the cell nuclei (figure 2). Excitation of PI (figure 3) occurs at ... ratio. The system detects PI-stained nuclei rather than individual cells. Because nuclei are virtually uniform in size regardless of cell ...

Neurite outgrowth cell-by-cell analysis using the IN Cell Developer Toolbox

... One fluorophore is used to mark the cell nuclei and the other is used to identify the entire ... counter-stained with Hoechst 33342 to identify nuclei and imaged using the IN Cell Analyzer 3000. ... Select the channel with the image of the nuclei marker (Fig 2). ...

Effectene Transfection Reagent provides efficient gene delivery to primary neuronal cell cultures

... with 200 ng DNA and 5 l of Effectene Reagent. nuclei (blue) of cells not reacting with MAP2 ... are glial-cell nuclei. Note that the nuclei of transfected neurons are not fragmented, which ... compartments of neurons (red). B Cell nuclei were stained using DAPI (blue). C ...

Genomic DNA Isolation Protocol(1,2,3)

... containing genomic DNA. I Isolation of nuclei nuclei from blood Caution: Wear suitable ... 5 minutes on ice. Pellet nuclei by centrifugation at 1000 x g for 12 minutes at ...

Principles and applications of electrical cell fusion

... outer cell membrane has fused, two or more cell nuclei are still present. The cell nuclei also fuse at a later point within the cell. In ... in the bovine embryo: assessment of donor nuclei and recipient oocyte, Biol. Reprod., 1987, ...

Genomic DNA Isolation Protocol(1,2,3)

... containing genomic DNA. I Isolation of nuclei nuclei from blood Caution: Wear suitable ... 5 minutes on ice. Pellet nuclei by centrifugation at 1000 x g for 12 minutes at ...

Principles and applications of electrical cell fusion

... outer cell membrane has fused, two or more cell nuclei are still present. The cell nuclei also fuse at a later point within the cell. In ... in the bovine embryo: assessment of donor nuclei and recipient oocyte, Biol. Reprod., 1987, ...

Radiology advance points way to non-invasive brain-cancer treatment

... while gadolinium is opaque. Then, where gadolinium is localized-in the nuclei of the cancer cells only-what's known as "the photoelectric effect" takes ... and consequently cell death, gadolinium atoms must be localized in the nuclei of cancer cells." Because of the deadly nature of GBM, De Stasio says ...

A new wide field-of-view confocal imaging system and its applications in drug discovery and pathology

... 21, 22 The commonly used hematoxylin and eosin staining colors the nuclei of cells blue-purple and other tissue components pink. 23, 24 Generally, ... 3 is a magnified view of part of Fig. 2, in which the round dark spots are nuclei of cancerous cells immunostained with the MIB-1 marker. Figure 4 is a ...

siRNA screening of the cell cycle with two dynamic GFP sensors (from Discovery Matters Issue 1, July 2005)

... sequence (NLS) within the PSLD retains the fusion protein within the nuclei of G1 cells. Phosphorylation of serine 967 within the PSLD by CDK2/cyclin ... for 24–48 h post transfection. Cells were fixed in 4% formalin and nuclei stained with DRAQ5 ™ (BioStaus).

Cellular ...

Visualizing siRNA in Mammalian Cells: Fluorescence Analysis of the RNAi Effect

... cell. Figure 7. siRNA Associated with nuclei of Dividing Cells. HeLa-S3 cells grown on cover slips ... filters. The siRNA (green) can be seen associated with each nuclei (blue) of a dividing cell. The localization of transfected ...

New Protocols for Isolating High- Molecular-Weight Genomic DNA

... the kit to remove cellular debris (<3 minutes), isolation of the cell nuclei by centrifugation (<20 minutes), and protease digestion of the nuclei (45 minutes). 1,2 The resulting suspension is transferred to a ...

The Role of Engineering in Health Science and Technology

... are creating next-generation medical diagnostics. The application of nuclear magnetic resonance, in which distributions of a given species of atomic nuclei are measured, is the basis of the powerful magnetic resonance imaging (MRI) technique in wide use today. The continuing exponential growth in the ...

Human stem cells stand on their own

... A colony of human embryonic stem cells grown over a period of 10 months in 2004 in the absence of mouse feeder cells. The cell nuclei are stained green; the cell surface appears in red. Photo courtesy of Ren-He Xu/UW-Madison. Madison, Wis. Tackling a pressing and controversial ...

Father of life patents downplays historic role

... for bacteria. Bacteria, he noted, have undergone 2.7 billion years of evolution, and as a result they know how eukaryotic cells - those with a nuclei and organs (or organelles) - function. They therefore appear not to be content with slowing the growth of cancer cells, but interfering with all of ...

Glossary for The BioPharm Guide to Fermentation and Cell Culture

... these pathogens are composed only of protein, with no detectable nucleic acid. prokaryotes Simple organisms with no cell nuclei and very few cell organelles. protein Macromolecules whose structures are coded in an organisms DNA. Each is a chain ...

DNA Damage and Repair - Quantification of Sub-Cellular Events Using Automated Confocal Imaging

... in the number and intensity of foci in H202 treated cells compared to control cells. The two foci shared a high degree of co-localization within nuclei as observed in the merged images. Preliminary results had shown that the nuclear foci were not evenly distributed in the same focal plane ( ...

Quantitative Analysis of the Cell Cycle in Imaging Applications

... cycle while it is still possible to drop in an additional specificity of interest to the user. For this assay, Hoechst 33342 was used to identify all nuclei and an anti-phospho-Histone 3 antibody was used to identify cells in M phase.1 Similarly immunofluorescent staining of incorporated bromodeoxyuridine ...

Laser Capture Microdissection (LCM) and Expression Profiling of Long-Range Projection Neurons

... circuits. References 1. Nottebohm, F., Kelley, D.B. & Paton, J.A. (1982) Connections of vocal control nuclei in the canary telencephalon. J. Comp. Neurol. 207(4):344-357 2. Kobbert, C., Apps, R., Bechmann, I., Lanciego, J.L., Mey, J., ...

Identification of cells in S phase using the Cell Proliferation Fluorescence Kit and IN Cell Analyzer 1000

... BrdU incorporation was determined as described in the Method section. Plates were imaged using the IN Cell Analyzer 1000. Figure 1 depicts nuclei of U2OS cells stained with Hoechst nuclear dye (blue). BrdU incorporation is shown in pink (Cy5). Cells in S phase are identified by their pink ...

Analysis of in vitro micronucleus assays using the IN Cell Analyzer 3000 Micronuclei Formation Analysis Module

...

2. Select a mitomycin C positive control well. Apply intensity threshold , erosion , and size filtration settings to identify nuclei in the blue (Hoechst) channel. Take care to exclude micronuclei from identified objects.

3. Adjust nuclear power (DNA content) and ...

The measurement of DNA condensation and mitochondrial changes during apoptosis and necrosis in cell based assays

... is applied sequentially to analyze the nuclear and cytoplasmic staining. This module automatically defines measurement areas of interest such as nuclei and cell bodies (Figs 1C and 1D), and then quantitates qualifying ‘granules’ of a specified dimension detected within the measurement ...

Transformation of Filamentous Fungi by Microprojectile Bombardment

... while conidia of A. nidulans are uninucleate (Bennett & Klich, 1992). Consequently, in a protoplast, only one of several nuclei might be transformed, thus resulting in a lower proportion of stable transformants compared to Biolistic transformation of ...

RNA Interference and Gene Silencing: History and Overview

... This was first demonstrated in Neurospora , when Cogoni and colleagues showed that gene silencing could be transferred between nuclei in heterokaryotic strains (1 , 7 ). It was later confirmed in plants when ...

Using Validated siRNAs in Functional Genomic Assays

... cells treated with TNFalpha, NF-kappaB translocates from its predominately cytoplasmic localization to become concentrated in nuclei where it interacts with DNA as a transcription factor (Figure 1). In cells transfected with TNFR siRNA, the translocation of NF-kappaB ...

Protect RNA and Improve Target Cell Identification

... Intercalating Stain) Acridine Orange stain intercalates into nucleic acids to enhance visability of nuclei in clusters of cells (Figure 3). Nuclear-rich epithelial tissues, such as clusters of malignant cells, are easily identified ...

Drive Long-Term Silencing with CMV Vectors

... the resulting reduction in protein expression was examined by fluorescence microscopy. The cells were mounted with DAPI to stain nuclei (Blue). Images were digitally captured and quantified using Metamorph software. The p Silencer 4.1-CMV ...

Efficient Cellular Fractionation for RNA and Protein Isolation

... by nonionic detergents. After selective lysis of the plasma membrane in Cell Fractionation Buffer, intact nuclei are collected by a quick centrifugation step and resuspended in Cell Disruption Buffer. The supernatant ...

High-content screening of GPCR activation with MetaXpress, AcuityXpress and the Transfluor assay system

... The MetaXpress Transfluor Module uses the proprietary Adaptive Background Correction system to distinguish pits, vesicles and nuclei from their local surroundings so they can be selectively measured and counted, even in the presence of uneven staining or protein ...

New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter

... available as stand alone products. These vectors allow real-time monitoring of the fate of peroxisomes, mitochondria, golgi and nuclei in both transient and stable applications. Fig.3 Useful hrGFP-Based Controls for Retroviral Expression ...