Mutagenesis in Biological Technology

Frost & Sullivan Recognizes Cisbio Bioassays for Technology Innovation

... technologies. "The determination of ligand binding affinity has been used to delineate the ligand binding site of GPCRs through a site-directed mutagenesis strategy," says Frost & Sullivan's Dr. Sudeep Basu. "These studies also provide clues to the activation mechanism of the receptors." It is ...

Frost & Sullivan Awards Precision Biosciences for Technology Innovation and Molecular Design Process in the Genomics Market

... an amalgamation of core enzyme catalysis, high-specificity DNA targeting, computational molecular simulations for structural modeling, and rational mutagenesis to design a set of extremely specific 'molecular scissors' for the precise engineering of large genomes." The DNE technology is so specific that ...

Detection of Mutations in the CYP21 Gene Using the DCode System

... the sequence of interest. Site-Directed mutagenesis In order to obtain mutant control ... mutant controls were generated by site-directed mutagenesis at the following nucleotide positions: ... de Vos, W. M., Improved site-directed mutagenesis method using PCR, Nucleic Acids Res., 19 ...

A Multiple Mutation Model System as a Test Development and Training Tool for Denaturing Gradient Gel Electrophoresis

... mutations was created by site directed mutagenesis in an exon 4 containing clone. The ... Materials and Methods SITE-DIRECTED mutagenesis OF hMSH2 EXON 4 A series of ... primer resulted in the site-directed mutagenesis of the amplified product. As the ...

Randomize Gene Sequences with New PCR Mutagenesis Kit

... GeneMorph PCR mutagenesis kit produces a unique mutational ... The GeneMorph PCR mutagenesis kit simplifies the process of ... GeneMorph method is robust and can be applied to mutagenesis of long targets (1-10 kb). ...

High-Efficiency Site-Directed Mutagenesis of Large Plasmid Vectors

... XL: a highly versatile kit for site-directed mutagenesis of large plasmids Michael Borns Peter ... Stratagene The QuikChange site-directed mutagenesis kit* characteristically generates mutagenesis efficiencies exceeding 80%. We have created a new ...

Efficiently Insert Unique Restriction Sites into Plasmid Vectors

... Perform insertion mutagenesis in less than one day Lisa Breister Jeff ... vector using the QuikChange site-directed mutagenesis kit.* The insertion created three unique ... procedure the method of choice for most mutagenesis projects. Here we describe ...

Big Blue Rodent Cell Cultures for Mutagenesis Studies

... embryonic cell line are useful for in vitro mutagenesis studies. These cell lines are part of the Big Blue transgenic rodent mutagenesis assay system,* a system for detecting mutations ... analysis. In addition to short-term in vitro mutagenesis studies, these cell lines are useful for ...

Tissue Specificity for Mutation Parallels Tissue Specificity for Cancer

... available data to illustrate how the Big Blue transgenic rodent mutagenesis assay system is used in tissue-specific studies ( Table 1 ). ... Mutagen. 28: 418-423. Mirsalis, J.C., et al.(1993) mutagenesis 8: 265-271. Suzuki, T., et al. (1996) Environ. Mol. ...

Reliable Assessment of Micronuclei for Genotoxicity Testing in Mice or Rats

... FCM micronucleus assay with Stratagenes Big Blue Transgenic Rodent mutagenesis Assay*, researchers can concurrently evaluate the clastogenic and ... When used in conjunction with Stratagenes Big Blue Transgenic Rodent mutagenesis Assay, a test compound or treatment can be assessed for both mutagenic ...

A Positive Selection Assay for Mutation Analysis in Big Blue Animals

... way to perform mutational analysis in the Big Blue transgenic rodent mutagenesis assay system that selects for mutations in the cII gene of coliphage ... figure 1 Stratagenes Big Blue transgenic rodent mutagenesis assay system has been used extensively for studying mutations ...

The New Green Revolution offers hope to poor farmers and poor consumers

... to develop insect-resistant potatoes and sweet potatoes. An Austrian lab is working with researchers in Kenya to transfer technology on cassava mutagenesis and breeding. Cassava is a low-protein, starchy staple in regions where cereal grains cannot be easily grown. Monsanto, Pioneer, Astra-Zeneca and ...

Detection of Genetically Modified Soybean in Processed Foods Using Real-Time Quantitative PCR with SYBR Green I Dye on the DNA Engine Opticon 2 System

... C.S., Eichholtz, D.A., Frazier, R.B., Hironaka, C.M., Levine, E.B., Shah, D.M., Fraley, R.T. and Kishore, G.M. Site-directed mutagenesis of a conserved region of the 5-enolpyruvylshikimate-3-phosphate synthase active site. Journal of Biological Chemistry 266(33):22364-22369 ...

Enjoy a Greater Sense of Security with PCR-Grade Water

... to be addressed by the molecular biology field. In order to assign functions to the genes encoded in the enormous amounts of sequence data, mutagenesis methods are of paramount importance. By combining a powerful polymerase chain reaction (PCR) technique to generate linear expression fragments ...

PCR Grade Deoxynucleotides

... cost! Use PCR grade dNTPs in daily applications, including PCR, RT-PCR, cDNA synthesis, sequencing, DNA labeling, and mutagenesis reactions. Our new manufacturing processes ensure higher quality without increasing production costs. Therefore, you obtain ...

TKB1 Cells Identify Receptor Tyrosine Kinase Interacting Proteins

... in a phosphotyrosine-dependent and sequence-specific manner and, therefore, bind to short linear peptide sequences. 1,2,3 Extensive mutagenesis of known autophosphorylation sites in the RTK Neu/ErbB-2 has revealed that four (Y1144, Y1201, Y1227, Y1253) of the five autophosphorylation ...

New BL21-CodonPlus Cells Correct Codon Bias in GC-Rich Genomes

... products. Previously, the typical remedies for overcoming codon bias were to alter the codon specifications of a target gene by site-directed mutagenesis or to circumvent the problem by switching to a eukaryotic expression system. To make it easier to resolve the codon bias problem, Stratagene ...

Codon Bias-Adjusted BL21 Derivatives for Protein Expression

... recombinant genes can be salvaged either by synonymous replacement of the rare codons with more frequently used codons using site-directed mutagenesis or by supplementing the host with extra copies of the cognate tRNA genes. Now, Stratagene has generated BL21-CodonPlus-RIL chemically ...

Using XL1-Red Mutator Strain to Generate Esterase Variants

... which was expressed well in E. coli using a rhamnose-inducible promoter. Since the structure of PFE is not known, positions for site-directed mutagenesis are difficult to predict. Alternatively, a random mutagenesis of the gene combined with an assay system also allows detection of the desired ...

A New Lambda Vector for Mammalian Expression

... ExAssist helper phage R408, VCSM13, RE704, ExAssist helper phage ssDNA mutagenesis yes yes yes yes ...