'Enrichment of Genomic Loci for Targeted Sequencing Application' Is Focus of 2009 AGBT Conference Workshop

... Inc., will present " Enrichment of Genomic loci for Targeted Sequencing Applications , " to ... for isolating biologically relevant genomic loci on the megabase scale will be required," said ... Olson will present " Enrichment of Genomic loci for Targeted Sequencing Applications " at the ...

RainDance and Scripps to Collaborate on Targeted Sequencing for 'Wellderly' Study

... for amplifying a large number of genomic loci across a diverse set of samples. "Looking at ... of isolating biologically relevant genomic loci on the megabase scale. RainDance's Sequence ... a uniform representation across all targeted loci -- greatly increasing the capacity of STSI ...

Automated Sequence Capture for Next-Generation Sequencing Paves the Way for Personalized Medicine

... amongst geneticists: targeted re-sequencing of medically-relevant genome loci will greatly enhance the understanding of health and disease in crucial ... HybSelect will support the selection and enrichment of specific gene loci for Next Generation Sequencers. The new technology is available as a ...

RainDance Technologies Delivers RDT 1000, Sequence Enrichment Solution to The Genome Center at Washington University School of Medicine in St. Louis

... the GC, along with customized PCR primer libraries to selectively amplify loci of interest for their research initiatives. Elaine Mardis, Ph.D., ... in droplets enabling the amplification of hundreds to thousands of genomic loci in a single tube. The RainStorm format avoids the limitations of ...

RainDance Technologies to Present 'Sequence Enrichment Using Droplet-Based Microfluidics' Workshop at ASHG 2008

... techniques, a robust method for isolating biologically relevant genomic loci on the megabase scale will be required. Analogous to what the massively ... We will present the use of this novel method for enrichment of genomic loci with minimal selection bias prior to sequencing, demonstrating the utility ...

RainDance Technologies Delivers RDT 1000, Sequence Enrichment Solution to JCVI

... provide customized PCR primer libraries designed to selectively amplify loci of interest for research initiatives at JCVI. "The program provides an ... in droplets enabling the amplification of hundreds to thousands of genomic loci in a single tube. The RainStorm format avoids limitations of traditional ...

RainDance Technologies to Present Workshop on Sequence Enrichment Using Innovative Microdroplet-Based Solutions at ASHG 2008

... economically unrealistic. To exploit the full potential of new sequencing techniques, a robust method for isolating biologically relevant genomic loci on the megabase scale will be required. Analogous to what the massively parallel sequencing technologies have done to improve the efficiency of DNA ...

Use it or lose it? Researchers investigate the dispensability of our DNA

... the observation that the elements are rarely lost in any species," said McLean. "In fact, they are over 300-fold less likely to be lost than genomic loci which evolve neutrally in our genome." Bejerano explained that while loss of some elements may have a significant impact on the fitness of a ...

CombiMatrix and Clarient Collaborate to Market, Sell Novel Genomics-Based Test for Chronic Lymphotic Leukemia (CLL)

... diagnosis" in regard to CLL. The HemeScan test is an innovative approach to the management of CLL. In a single test, it assays all of the genomic loci for copy number aberrations currently measured by commercial multi-probe fluorescent in situ hybridization (FISH) kits. Moreover, by ...

SNPs Powerful Tools for Association Studies

... length at a locus is called an allele, and some loci can have more than ten alleles. The ... sibpair studies. Technically, genotyping STR loci requires sophisticated efforts because the ... the non-random association between alleles at two loci (Goldstein, 2001), and is primarily the result of ...

Fully automated DNA purification and efficient multiplex PCR for analysis of microsatellite loci

... simultaneous analysis of multiple microsatellite loci by multiplex PCR is highly desirable. ... the amplification of microsatellite loci that was used for this study. In this ... in multiplex PCR of microsatellite loci using the QIAGEN Multiplex PCR Kit. ...

QIAGEN Multiplex PCR Handbook

... Amplification of Microsatellite loci Multiplex PCR Protocol Using ... for Amplification of Microsatellite loci Important notes before starting ... template DNA, due to the fact that not all target loci are present in the reaction. This is ...

CastAway Precast Gels for Rapid Automated DNA Sequence Analysis

... the automated infrared DNA analyzer. In Figure 4 , four STRP loci are detected in each lane (four multiplexed reactions per channel). Expect 1,440 genotypes (60 lanes x 4 loci x 2 channels x 3 loads) for a typical throughput per precast ...

Genomic Analysis of Formalin-Fixed Paraffin Embedded (FFPE) Tissues through the use of Whole Genome Amplification (WGA)

... best predictor of a samples potential for successful amplification. 7 The assay employs multiplex PCR to amplify a number of differently sized loci within the human genome; loss of the higher molecular weight bands indicates that the DNA has degraded past the point where whole ...

Oragene and Whole Genome Amplification with GenomiPhi

... template for subsequent WGA reactions. Up to 4 re-amplifications have been performed with no apparent effect on PCR of various loci across the human genome (ref. 7). In summary, DNA collected with Oragene works well with the GenomiPhi WGA kit and has the ...

Using the DCode System to Identify DNA Sequence Variation for Studies of Population Structure in Marine Organisms

... sequence information available to the investigator. When sequence data are unavailable, as was the case for the two tautog loci described above, perpendicular DGGE of the PCR product is necessary to define the appropriate gradient for parallel ...

Rapid SNP Genotyping of the Pharmacogenomically Important Allele CYP2D6*4 Using Real-Time qPCR on the DNA Engine Opticon 2 System

... of the probe substrate versus the actual drug. 1 For the genotyping method, the SNPs are characterized at one or more loci within the CYP2D6. The SNP genotype provides an accurate and reliable prediction of CYP2D6 activity. 5 The genotyping method ...

Detection of Variation in Highly Polymorphic Mhc Genes by Denaturing Gradient Gel Electrophoresis Using the DCode System

... to maximize the throughput of the DCode system, we conduct the A1 analysis on daytime runs (at 120 V max) and analyze other loci on overnight runs. We use a single brand of acrylamide for analysis of each gene locus, and run a time series on new batches of ...

SEESNP DISCOVERY

... our service facility apart include: SPRItechnology for increased sequencing performance and more accurate SNP loci identification Proprietary assay design software for fully automated amplicon modeling Extensive assay validation and ...

Custom and library siRNA for efficient gene silencing

... Microsatellite Analysis with Optimized QIAGEN Protocol Figure 2 Analysis of microsatellite loci D3S1358, TH01, D21S11, D18S51, and Penta E was carried out using 1 ng of K562 human genomic DNA and fluorescein-labeled primers. ...

QIAGEN Multiplex PCR Kit

... Analysis with Optimized QIAGEN Protocol Analysis of microsatellite loci D3S1358, TH01, D21S11, D18S51, and Penta E was carried out using 1 ng of K562 human genomic DNA and fluoresceinlabeled ...

Easily Amplify Genomic DNA with Long-Distance PCR

... can be realized. This procedure will have wide application in transgenic mice technology and may be useful for detailed analysis of chromosomal loci with extensive modifications, either introduced by homologous recombination or naturally occurring (i.e., deletions and insertions). ...