Linker in Biological Technology

DNA is blueprint, contractor and construction worker for new structures

... single-stranded portion of this DNA serves as the linker DNA, which seeks out a complementary single ... The binding of the two single strands of linker DNA to each other completes the double helix, ... structure -- a crystal. One sequence of linker DNA, programmed by the researchers, results in ...

Medarex Announces Allowance of Investigational New Drug Application for Company's First Antibody-Drug Conjugate, MDX-1203

... linker. Medarex believes that its ADC technology overcomes many of the key development challenges of drug conjugates, including issues of linker stability, potency and multi-drug resistance while maintaining a wide therapeutic window with minimal toxicity. Medarex has conducted multiple ...

Transgenomic Awarded SBIR Grant to Support Development of SURVEYOR(R) Endonuclease Adaptor-ligated Libraries (SEAL) for Determining Whole Genome Sequence Variation

... for whole genome analysis under development by Transgenomic. It identifies DNA variations between a reference genome and a test genome. Using a linker that attaches an identification tag of known sequence to the DNA ends processed by the SURVEYOR Nuclease, SEAL generates a library of genetic ...

Transgenomic Introduces SURVEYOR(R) Endonuclease Adaptor-Ligated Libraries (SEAL) for Determining Whole Genome Sequence Variation

... for whole genome analysis under development by Transgenomic. It identifies DNA variations between a reference genome and a test genome. Using a linker that attaches an identification tag of known sequence to the ends cleaved by the SURVEYOR Nuclease, SEAL generates a library of genetic variations ...

Taxol bristle ball: a wrench in the works for cancer

... they needed to perform to create the ball. Using the wrapped version of the drug, they undertook a series of reactions to attach the drug to linker molecules that were, in turn, attached to the ball. In the final step of the reaction, they dissolved the wrapper, restoring the key. "We are ...

Monitoring fluorescence resonance energy transfer (FRET) between GFP fusions in lysates of the yeast Saccharomyces cerevisiae using the Varian Cary Eclipse

... GFP linked by a 27 amino acid peptide linker that contains a recognition site for ... 25C to promote cleavage of the peptide linker by trypsin (Figure 2). Using the Scan ... addition of trypsin, which cleaves the peptide linker that tethers the GFPs. A small ...

Inactivation of Contaminating Templates in PCR Using High Intensity Ultraviolet Light

... sterilization method using the GS Gene linker UV chamber to destroy contaminating amplicon or ... placed in the middle of the GS Gene linker oven and were either capped and placed on their ... UV light was delivered by the GS Gene linker chamber for 30 minutes. After UV irradiation was ...

UV Nicking of DNA in Agarose Gels for Enhanced Transfer and Detection of Megabase Size DNAs

... to HCl nicking. The GS Gene linker UV chamber contains preprogrammed routines for ... with 60 mJoules of energy in the GS Gene linker UV chamber after staining with ... on a glass tray for transportation to the GS Gene linker chamber, place the tray inside the GS ...

Synthesize High Yields of Biotinylated aRNA

... single biotin UTP label. While various linker arm sizes (e.g., Biotin-11-UTP, Biotin-16-UTP) did ... UTP incorporation into aRNA during IVT, the longer linker arms slightly impeded subsequent purification of the ...

Genomic Analysis of Formalin-Fixed Paraffin Embedded (FFPE) Tissues through the use of Whole Genome Amplification (WGA)

... relatively short lengths of nucleic acid stretches, ~1kb or less. Four WGA methods have been described and are in common use they include linker adapter PCR (LAP) 8-10 , primer extension preamplification PCR (PEP) 11 , degenerate oligonucleotide PCR (DOP) 12,13 and multiple strand ...

Double-Affinity Purification of GST-His Tagged Proteins Using Vivapure 8-to-96 Well Cobalt-Chelate Kit

... steps. Conclusions Our results show that a protein construct expressed as a GST-HIS fusion with a protease recognition site as a linker is suitable for being quickly purified using two affinity columns. This protocol provides the possibility to eliminate contaminant native proteins ...

Efficient and Reliable Linear Amplification of cRNA

... T7 RNA polymerase. For first-strand cDNA synthesis an oligo(dT) primer linked to the T7 promoter sequence is used. In the next step, this T7 linker allows the linear amplification of the template and the simultaneous incorporation of labeled nucleotides with T7 RNA polymerase, generating ...

Quantification of Nucleosomes in Serum by the Cell Death Detection ELISAplus

... are elementary units of chromatin formed by an octamer of histones and 146 bp DNA wrapped around it. The nucleosomes are connected by linker DNA of about 15 -100bp which are the preferential binding sites of the endonucleases [9-11]. Under physiological conditions, nucleosomes are ...

The DIG System Nonradioactive and Highly Sensitive Detection of Nucleic Acids

... year. Well-established protocols (many years of experience) Easy stripping and reprobing of Southern blots (alkali-labile DIG linker for DNA probes) Special protocol for easy stripping of Northern blots The DIG System allows the safe and ...

An Optimal DNA Microarray Substrate for the Identification of Fetal and Somatic Liver Stem Cells of the Rat

... range of measurable signals is very high. Binding the probes (in this case: 50mer oligonucleotides) terminally, modified with a linker molecule (5'aminoalkane molecule), to the slide surface results in the advantage that the entire length of spotted oligonucleotides ...