Knock in Biological Technology

A supercharged metal-ion generator

... energetic ions atoms with a positive charge to knock the metal atoms off a target. Scientists at the ... themselves ionized and return to the target to knock off still more atoms. The great advantage of ... themselves been ionized return to the target to knock out yet more target atoms. Some of the sputtered ...

Exelixis Sells 80% Stake in Artemis to Taconic

... by the ArteMice(TM) platform include knock out and "humanization" platforms and platforms for generating inducible/reversible RNAi knock down models and ASKA models for the inducible and reversible knock down of kinases. These platforms are being ...

Sigma-Aldrich Introduces MISSION(R) esiRNA for Low Cost, High Specificity Pooled RNAi Screening

... marketing manager for Functional Genomics at Sigma-Aldrich. "The pooled format, unlike anything else on the market, should enable researchers to knock down genes rapidly and cost-effectively. This is an exciting addition to our current MISSION lineup and provides an effective technology that will ...

Sangamo BioSciences Reports Fourth Quarter and Full Year 2008 Financial Results

... December, Sangamo and Pfizer Inc entered into an agreement to provide Pfizer with a worldwide, non-exclusive license for the use of ZFN reagents to knock out a single gene in CHO cells and for the use of such cell lines for clinical and commercial production of therapeutic proteins. Under the terms of ...

Combating infection of crops by nematodes is soon to improve

... through manipulation, are not able to produce PIN1, PIN2, PIN3, PIN4 or PIN7. In this way, the researchers have been able to show that nematodes knock out certain of the plant's PIN proteins, while other PIN proteins are activated just to transport auxin to the neighboring cells. With this ...

Open Biosystems' Open Access RNAi Program Adopted by University of Copenhagen and University of Queensland

... research groups access to this valuable genomic screening resource. This will allow our researchers to conduct whole genome or customized gene set knock down experiments that will greatly facilitate discovery in our cell, developmental biology and cancer research programs." "The lentiviral shRNAmir ...

Thermo Fisher Scientific Unveils New Laboratory Technologies at Analytica 2008

... RNAi Technology Thermo Scientific Dharmacon Accell(TM) siRNA -- RNA-interference (RNAi) is a powerful technology used to silence, or knock down, target genes and the proteins they produce, revealing their role in biological pathways and disease processes. Accell is a breakthrough ...

Taming the anthrax threat

... effects during the 60 days people need to take them. Many people do not complete the full course of treatment. An improved drug would knock out the anthrax microbe but leave the normal good bacteria in the gut alone. As a first step, U-M scientists plan to screen compounds to find ones ...

Taming the anthrax threat

... effects during the 60 days people need to take them. Many people do not complete the full course of treatment. An improved drug would knock out the anthrax microbe but leave the normal good bacteria in the gut alone. As a first step, U-M scientists plan to screen compounds to find ones ...

WTN Interview: Carl Gulbrandsen
WARFs Investment and Portfolio Strategy

... we have invested in Venture Investors and Baird Venture Partners in Wisconsin, and when other VC companies doing their fundraising rounds, they knock on our doors. Well do the diligence like we do with the others. Like any institutional endowment, we have a target allocation model of how much is ...

Pity the Midwest Technology Sales Guy

... are excellent that you'll eventually be dissatisfied with the company. If the opportunity passes all these tests, you're in great shape. Go knock 'em dead! ...

UW discovery could lead to new TB treatments

... cell walls. Enzymes are proteins that initiate chemical reactions within plant and animal cells. "We've figured out how this enzyme works. If you knock it out, the bacteria aren't viable," Kiessling explains. "It's an essential enzyme." The TB microbe's success and resistance to traditional drugs ...

One year after the 2003 blackout: Is Wisconsin prepared?

... network of America: there are thousands of transmission lines that have no real protection, power nodes that are critical to operation and easy to knock out, and generators that are located far from the users and repair methods. Heins suggests the best way to prevent a reoccurrence of last years ...

Security concerns grow with mobile tech devices

... challenge. It's not that all of the data stored on a mobile device needs to be encrypted - only the clichd mission-critical data need apply. The knock on encryption is that entering the required codes can impact the speed and usability of the mobile devise, and discourage users from using it. The ...

A Screen of shRNAs Targeting Tumor Suppressor Genes to Identify Factors Involved in Paclitaxel Sensitivity

... cell sensitivity to the widely used cancer therapy drug, Paclitaxel. Lentiviral particles were used to transduce cells in 96-well plates and knock down the corresponding tumor suppressor gene expression. Cells were split into two samples. One sample set was treated with Paclitaxel and the ...

RNA Interference and Gene Silencing: History and Overview

... PTGS and, in particular, RNA interference (RNAi) PTGS initiated by the introduction of double-stranded RNA (dsRNA) as a tool to knock out expression of specific genes in a variety of organisms (reviewed in 1-3 ). How was RNAi ...

YOUR DATA: Direct Real-time RT-PCR on Sympathetic Neuron Lysates Silencing of Focal Adhesion Kinases with Silencer Validated siRNAs

... each of these kinases, Dr. Joseph Loftus employed Silencer Validated siRNAs targeting PTK2 and PTK2B to knock down the expression of each of these kinases. As seen in Figure 2, the expression of PTK2B was ...

YOUR DATA: Direct Real-time RT-PCR on Sympathetic Neuron Lysates Silencing of Focal Adhesion Kinases with Silencer Validated siRNAs

... each of these kinases, Dr. Joseph Loftus employed Silencer Validated siRNAs targeting PTK2 and PTK2B to knock down the expression of each of these kinases. As seen in Figure 2, the expression of PTK2B was ...

More siRNA Vectors for RNA Interference

... This vector was developed by Sui and colleagues at Harvard Medical School and has been successfully used to knock down expression of cdk-2 and lamin A/C in HeLa, H1299, U-2 OS and C-33A (cdk-2 only) cells (Sui et al., 2002). Ambion's latest ...

Optimize Transfection of siRNAs for RNAi

... and target. Too much siRNA may result in cytotoxicity. Conversely, if too little siRNA is transfected, gene target knock down may be undetectable. Experiments Addressing Transfection Variables Several ...

Matched siRNAs and Assays: Ambion + Applied Biosystems = RNAi Success

... than Northern analysis. siRNA Validation Gene silencing experiments require siRNAs that efficiently knock down expression of the target gene. To prove that a particular siRNA sequence is effective, the siRNA needs ...

Optimizing Chemical Transfection and Electroporation of siRNAs

... or poor complexing with the transfection agent. Conversely, if too little is transfected, gene target knock down may be undetectable. Concentrations of siRNA typically used for transfection range from 1 nM to 100 nM ...

Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*

... siRNA directed against microtubule-associated protein 2 (MAP2). RNAi has become a powerful tool used to knock down the expression of genes in cultured mammalian cells. However, neuronal cells, which are difficult to manipulate, have seemed ...

Custom and library siRNA for efficient gene silencing

... RNA transfection reagent. TransMessenger Transfection Reagent from QIAGEN efficiently delivers siRNA to cells for successful gene knock down (Figures 1 and 2). Once inside the cell, current models suggest that siRNA interacts with endogenous cellular proteins to form ...

BioRobot MDx standardize your automated protocols in clinical laboratories

... reagent. TransMessenger Transfection Reagent from QIAGEN efficiently delivers siRNA to cells for successful gene knock down (Figures 1 and 2). Once inside the cell, current models suggest that siRNA interacts with ...