kit

New Microarray Labeling Kit for Preparing cDNA Probes

...of identifying differentially expressed genes. The kit includesseveral control mRNA and two Control Micro...aliana mRNA provided in the probe-labeling kit to the probe-labeling reaction. The amounts of A. thaliana photosystem I chlorophyll a/b-bin...

A Positive Selection Assay for Mutation Analysis in Big Blue Animals

...ive and easy-to-use l Select-cII mutation assay kit A Positive Selection Assay for Mutation Analysis...coliphage lambda. The l Select-cII mutation assay kit uses an hfl E. coli strain and selective temperature conditions to provide anenvironment where on...

Purified Single-Stranded M13 DNA for Automated Sequencing

...tratagene The StrataPrep M13 DNA purification kit allowseasy, quick, and reliable purification of ba... Conclusion The StrataPrep M13 DNA purification kit contains a fast, easy, and reliable method for M13 purification. Recover single-stranded DNA from ph...

New Mammalian Two-Hybrid System Detects Protein-Protein Interactions

...e the capability of the mammalian two-hybrid assay kit for studying protein-protein interactions,...orking principle of the mammalian two-hybrid assay kit is similar to that used in Fields yeast two-hybrid system, except that the assay det...

New Kit Generates Exceptionally Pure Total RNA for RT-PCR

...g the Absolutely RNA RT-PCR miniprep kit Karen Dolter Jeff Braman St...-PCR. The Absolutely RNA RT-PCR miniprep kit eliminates contaminating DNA and produces highly pure total RNA qualified for RT-PCR ...

Highest Transfection Efficiency of an Endotoxin-Sensitive Mammalian Cell Line

...fection efficiencies with StrataPrep midiprep kit Scott Basehore Jeff Braman Stratagene Plasmi...tratagenes StrataPrep EF plasmid midiprep kit, a kit based on anion exchange chromatography, magneticbeads, and cesium chloride density gradient centrifu...

Detect Single Nucleotide Sequence Changes with Mx4000 Molecular Beacon Allelic Discrimination Kits

...se-pair deletion in the humanCCR5 (D32) gene. Each kit includes twoallele-specific molecular beacons in a... 1 ). The Factor V (Leiden) allelic discrimination kit is used to detect a common point mutation (Factor V Leiden) in the human Factor V gene. ...

Automated Fluorescent-Tag Cycle Sequencing

...RISM dye terminator cycle sequencing readyreaction kit was used to sequence the kits control plasmid, the pGEM -3Zf(+) vector, using the (-21) M13 forward primer. Both experiments wereperformed according to the manual supplied with the ABI PRISM sequencing kit.Following the completion of cycle sequencin...

Brilliant Core Reagent Kits for QPCR and QRT-PCR

... dUTP only (UNG not included in single-step kit Separate Tube 200 ...cribe RT dUTP only for single-tube kit In Buffer 200 $700 $3.50 ...

Genotyping Single Nucleotide Polymorphisms with Molecular Beacons

...carried out with the Super QUIK-GENE DNA isolation kit (Analytical Genetic Testing Center). Each 50- m l PCR reaction contained 100 ng of human genomic DNA, 500 nM molecular beacon A or G, 400 nM of each primer, 0.2 mM dATP, 0.2 mM dCTP, 0.2 mM dGTP, 0.4 mM dUTP, 3.5 mM MgCl 2 , 2.5 U of AmpliTaq Gold ...

Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins

... Protein Dipstick is an easy-to-use assay kit for detecting FLAG epitope-tagged fusionproteins. To visualize results, the kit uses a colloidal-gold (CG) labeledanti-FLAG M2 monoclonal antibody in a dipstick format. This highly...

New Yeast Cloning System for Producing Proteins with Native Amino Acid Sequences

...uture analysisof the protein. The ESP LIC cloning kit contains pretreated, ready-to-use pESP-2 vector, P...smids. The ESP LICcloning and protein purification kit includes all of these components plus S.pombe competent cells (strain SP-Q01), YES media for vegetat...

Luminol-enhanced Assay for Superoxide Anion (O2 face="Symbol" )

...uperoxide anions in cell culture. In addition, the kit providesa specific enhancement reagent to amplify ...usions LumiMax is a simple, sensitive, and unique kit for researchers performing luminol-enhanced chemiluminescent assays. It has many applications includ...

Fast and Easy Isolation of PCR-Ready Genomic DNA from Whole Blood

... Fig.1 The key to the DNA Stat blood kit is the unique design of the spin cups, whi... comparing contemporary protocols with Stratagenes kit (Table 1), the DNA Stat method demonstrates several advantages: no pelleting of WBC ...

Enhanced PCR Cloning System

... two new products: The StrataPrep PCR purification kit 6 makes it easy to prepare PCR products, and XL10-...f multiple samples. The StrataPrep PCRpurification kit removes primers by centrifugal washing; the desired product iseluted in a small volume of buffer or ...

Efficiently Insert Unique Restriction Sites into Plasmid Vectors

...rescue andsubcloning into specialized vectors. The kit relies on high-fidelity PfuTurbo DNA polymerase,*...ed using the StrataPrep plasmid miniprep kit 3 ), QuikChange 10X reaction buffer (5 l), primers (1 l of each at a concentration of 125 ng...

prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification

...-freewater] using total RNA or poly(A) + RNA. The kit also includes allreagents necessary for subsequent...ility are attainable. Useone of the two convenient kit formats, the Ultra HF RT-PCR system or the HFOne-Tube RT-PCR system, to fulfill a variety of RNA amp...

pfuturbo DNA Polymerase: A High-Performance, High-Fidelity Enzyme Ideal for PCR Cloning

...t can be easilycloned using the PCR Script cloning kit 2 we assumed PfuTurboDNA polymerase would also po...st purified using the StrataPrep PCR purification kit 8 to remove Taq DNA polymerase,primers, PCR contaminants, and buffer components. For the polishing ...

Reliable Assessment of Micronuclei for Genotoxicity Testing in Mice or Rats

...nd less cost compared to conventional methods. The kit requiresno special equipment and can be used for r... Analysis Kit to obtain genotoxicity data. The kit assembles all the quality-controlled supplies and reagents, allowing researchers worldwide t...

Performance Comparisons of Commercial RT-PCR Systems

...T-PCR system and the Titan One-Tube RT-PCR kit consistently amplified the longer 3.6-kb (Panel B)...erefore,realizing optimal performance of the Titan kit is limited to customers withtemperature cyclers that can be programmed in this fashion. Maximum perf...

Cell Proliferation Kit Outperforms the Competition in the Range of Cells Typically Counted

...accurate using the Quantos cellproliferation assay kit Leslie S. Arrington Genetic Applications LLC ...ationassay product. The data demonstrated that the kit more accurately assessed cellnumber in the range of cell number frequently found in commonly used cu...

High-Efficiency Site-Directed Mutagenesis of Large Plasmid Vectors

QuikChange XL: a highly versatile kit for site-directed mutagenesis oflarge plasmids M... efficiencies exceeding 80%. We have created a new kit formutagenesis of large plasmid templates. The QuikChange XLsite-directed mutagenesis kit has bee...

New Protocols for Isolating High- Molecular-Weight Genomic DNA

...uires very little hands-on time to perform.Use the kit to minimize physical manipulation of the DNA the p...less than 24 hours. The RecoverEase DNA isolation kit is designed to isolate veryhigh-molecular-weight genomic DNA by reducing the shearing forces that ta...

A Simple and Rapid Technique to Purify High-Quality Midiprep DNA

...ammalian cell transfection. StrataPrep midiprep kit purification of plasmid DNA 1 is based on alkaline... Use the fast, easy StrataPrep EF plasmid midiprep kit to isolate high-purityendotoxin-free plasmid DNA without resin slurries, toxic chemicals, orspeciali...

High-Throughput Isolation of Total RNA

...ays using the RiboGreen RNA quantitation kit (Molecular Probes) since absorbance measurements ...atesusing the StrataPrep 96 total RNA purification kit and the RNeasy 96 total RNA isolation kit from Qiagen. RNA isolations were performed accordingto ea...

Organize Your Data from the Big Blue Mutation Assay

...2 and the l Select-cII mutation assay kit 3 . Once the screening method is chosen, the corresponding fields specific to the particular assay are presented. On the Screening page, mutant information is entered. After DNA sequence information is available, the specific ...

A New PCR-based Mycoplasma Detection Method

... product from a single copy gene. In addition, the kit contains an internalcontrol template that serves as a positive control for PCR. The kit offers arapid and sensitive means to determine information about mycoplasma (and relatedacholeplasma...

High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli

...-FLAG and JNK insert were combinedaccording to the kit instructions and transformed into XL1-Blue superco... Stratagene has improved the Affinity LIC cloning kit by the addition of a more versatile vector, pCAL-n-FLAG. This vector has the same features as its pr...

Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications and Agarose Gels

...DNA prepared with the StrataPrep plasmid miniprep kit is easily digested with restriction enzymes, istra...pin cup in the StrataPrep plasmid miniprep kit yields up to 20 g of purified plasmid DNA from overnight bacterial cultures of 1.5 to 3 ml. ...

Simple, Sensitive Isotyping of Mouse Monoclonal Antibodies

...enes IsoDetect mouse monoclonal antibody isotyping kit is asimple, rapid and sensitive method for identif...enes IsoDetect mouse monoclonal antibody isotyping kit is a rapid method for determining the isotypes of mouse monoclonal antibodies. This ...

Isolate and Analyze Total RNA from Cells Harvested by Laser Capture Microdissection

... StrataPrep total RNA microprep kit for very small samples Karen Do...amples. The StrataPrep total RNA microprep kit provides an efficient method to isolate high-quality total RNA from small cell samples. ...

Fluorescence-Based Single-Tube Assays to Rapidly Detect Human Gene Mutations

...factor II (prothrombin) gene and the HFE gene.Each kit includes two allele-specific molecular beacons, th... 1 ). Convenient Single-Tube Format Each assay kit includes two allele-specific molecular beacons with an exactsequence match to each of the two target...

Versatile Reporter Vectors for Monitoring Viral Transduction

...he Transfection MBS mammalian transfection kit (Stratagene), modified according to Pear et. al. 4 Figure 2 shows that HeLa cells transduced with the virus were highly fluorescent and readily detectable by fluorescence microscopy. To determine transduction efficiencies for ...

Yeast Protein Production System Features High Yields and One-Step Purification

...or either inducible or constitutiveexpression. The kit includes easytotransform E. coli and yeast competent cells,glutathione agarose beads for protein purification, yeast media and timesaving,wellcharacterized procedures. Acknowledgments We thank Paul Russell and Janet Letherwood of the Research Insti...

Simple Isolation of RNA from Tissue and Cultured Cells

...an Stratagene The StrataPrep total RNA miniprep kit efficientlyisolates high-quality total RNA from 10... or water, it is ready to use. We showed that the kit is capable of high performance using a wide range ofstarting materials, both tissues and cells. We t...

Enzyme Immunoassay for Studying Intracellular Levels of cAMP

Sensitive, nonradioactive cyclic-AMP detection kit and adenylate cyclase(AC) toxin Danny Q. Hoang ... highly sensitive and specificantibody-based assay kit for nonradioactive, quantitative measurement ofintracellular levels of cAMP. The assay is based on t...

Detect Released Oligosaccharides Using the Eagle Eye II Still Video Imaging System

...abeledwith ANTS using reductive amination (Bio-Rad kit procedure). The FACE/ANTS gelfluorophore-tagging method is previously described. 3 Oligosaccharide PAGE The gel used came preformed in the Bio-Rad N-linkedprofiling kit. The gel contained 8-aminonaphthalene-1,3,6-trisulphonate (ANTS 4 )-labeled,...

pFB-ERV: Retroviral Delivery of the Ecdysone Receptor Proteins

...icult transfections when using thecomplete control kit Peter Vaillancourt Katherine A. Felts Stratagene We describe the vector pFB-ERV, an MMLV-based replication-defectiveretroviral vector for delivery of the ecdysone receptor proteins RXR and VgEcR.In stable cell lines infected with an estimated sin...

Constructing Directional cDNA Libraries from Limited Amounts of RNA

...ciency cDNA libraries in a plasmidvector. Use this kit with either enriched poly(A) + RNAor as little a...fRNA required when using conventional methods. The kit is based on an approachthat combines linker-mediated polymerase chain reaction (PCR) for amplifyingc...

Randomize Gene Sequences with New PCR Mutagenesis Kit

GeneMorph PCR mutagenesis kit produces a unique mutational spectrum ... The GeneMorph PCR mutagenesis kit simplifies the process of constructing mutant libraries for protein evolution and...

High-Titer Retroviral Vectors for Gene Delivery

...he Transfection MBS mammalian transfection kit (Stratagene) with some modifications. 4 Two days following transfection, viral supernatants were collected, and serial dilutions of each supernatant were used to infect dividing NIH3T3 cells. Two days later, the infected NIH3...

Optimizing pfuturbo DNA Polymerase Amplification Reactions with Perfect Match PCR Enhancer

...Stratagenes Opti-Prime PCR optimization kit. This kit consists of a set of buffers and PCR-enhancingagents (including formamide, DMSO, and Perfect Match PCR enhancer) for use in aneasy, two-step protocol to optimize difficult PCR amplification reactions. 6 REFERENCES Hogrefe, H. et al. (1997)...

Unique Enhanced DNA Polymerase Delivers High Fidelity and Great PCR Performance

...erase was also compared to the Advantage -GC2 PCR kit (CLONTECH). Amplification was carried out as recom...ying the300-bp target using the Advantage-GC 2 PCR kit (data not shown). Therefore, Herculase DNA polymerase can be used with the provided DMSO toamplify ...

Tools for Detecting MSH2 Expression in Chinese Hamster Ovary Cells

...gene.First, the StrataPrep total RNA miniprep kit isused to isolate ample yields of high-quality RNA... sets for RT-PCR, the StrataPrep PCR purification kit or the StrataPrep DNA gel extraction kit,the Prime-It II random primer labeling kit,NucTrap p...

High-Throughput System Generates Ultra-Pure PCR Products Suitable for All Applications

... Phenogenex The StrataPrep 96 PCR purification kit is a newmember of the StrataPrep product line. This kit offers a high-throughput formatfor purifying ultra-pure PCR product DNA from large numbers of sample...

Mammalian and Bacterial Expression in One Vector

...using Stratagenes Affinity CBP fusion detection kit and Calmodulin Affinity Resin, respectively. The n...croscopy. Stratagenes Seamless cloning kit 4 was used to amplify the GFP gene from the original TU58 5 construct using high-f...

Mammalian Expression Vector for Efficient Cloning of PCR Fragments

...efficient PCR cloning with pcmv-script PCR cloning kit Tanya Hosfield Kerstien Padgett Tim Sanchez ...n in mammalian cells. The pcmv-script PCRcloning kit uses a PCR cloning method that features a one-hourligation and doesnt require adding extra bases ...

Easy Cosmid DNA Purification with StrataPrep Kits

...he amount of purified DNA required determineswhich kit to use. StrataPrep purified cosmid DNA can serve as the sourcematerial for subclone library construction used in shotgun sequencing projects. 4 REFERENCES Basehore, S. and Braman, J. (1998) Strategies 12: 5-7. Braman, J. and Basehore, S. (19...

New Competent Cells for Highest Transformation Efficiencies

...b) from Stratagenes Affinity LIC cloning kit was digested and prepared for the ligation-independent cloning (LIC) reaction using standard conditions. 2 The kanamycin-resistance gene was annealed to the pCAL-n-EK vector. An aliquot of the annealed mix was used to transfo...

Fast Isolation of Total RNA from Small Numbers of Cells

...n Stratagene The StrataPrep total RNA microprep kit provides aconvenient method for isolating total RN...es recently released StrataPrep total RNA miniprep kit isdesigned to isolate total RNA from cells ranging from 10 5 to 10 7 cells. However, when you need ...

High-Efficiency Transfections Achieved with New Low-Toxicity Reagent

...ratagenes in situ b-galactosidase staining kit to stain cells with X-gal, and the resulting cells that expressed for b-galactosidase were photographed ( Figure 2 ). Fig.2 Assessing Post-transfection Cell Viability We evaluated the cytotoxic effects of GeneJammer...

Restriction Digests of DNA Isolated with the Perfectprep Gel Cleanup Kit

...ations. Eppendorfs Perfectprep Gel Cleanup kit is used to purify DNA fragments of various ...ed DNA. The Perfectprep Gel Cleanup kit was used to purify a 3.49 kb PCR1 fragment amplified from bacteriophage Lambda. The purified...

Eppendorf Perfectprep Gel Cleanup Kit

...ning using DNA purified with the Eppendorf kit was proven to be highly efficient. Subsequent restriction digests on plasmid DNA isolated from white colonies verified that the colonies indeed contained the correctly ligated insert and vector (data not shown). This indicates...

Eppendorf Perfectprep Gel Cleanup Kit

...rf kit. In sequencing and % recovery the Eppendorf kit out-performs the competitor in downstream applications. *This product is sold under licensing arrangements with F. Hoffmann-La Roche Ltd., Roche Molecular Systems, Inc. and Applied Biosystems....

TripleMaster PCR System

... safety data sheets which are available for this kit on line at www.eppendorf.com/msds ...ature freezer. If stored as recommended, the kit is stable at least until the expiration date stated on the label. 7 Q...

Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood Mini Kit

...equently be used in PCR1. The Perfect gDNA kit has been validated for use with several types of swabs, and will most likely work with others. The resulting gDNA is suitable for use in the main downstream applications such as PCR. Materials ...

Genomic DNA Extraction from Buffy Coat Using the Perfect gDNA Blood Mini Kit

...l. In addition, it has been shown that the kit can also be used to effectively purify gen...ied out to validate the performance of the kit when using buffy coat. Volumes ranging from 20 l to 200 l of buffy coat were taken from an e...

EppendorfPerfect gDNA Blood Mini Kit

...cessfully in Trans-PCR (Fig. 2). Thus, this kit can be used for the detection of C. burnetii in di...nated with Chlamydia and treated with this kit (data not shown). References Baca, O.G. and Paretsky, D. 1983. Q fever and...

Higher gDNA Concentrations with the Perfect gDNA Blood Mini Kit by Varying the Final Elution Volume

...al effects on downstream applications. The kit was tested with blood from three different individuals using three different preservatives. Materials Perfect gDNA Blood Mini Kit Required but not supplied: Ethanol (95%100%); ae...

Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit

... 1x PBS (sufficient amounts are supplied in the kit for blood work, but additional amounts m...uals using both the Perfect gDNA kit and the leading competitors kit gDNA was extracted from the saliva of two individu...