Inoculation in Biological Technology

Tips from the journals of the American Society for Microbiology

... the mouse intestine for 2 months following intragastric and subcutaneous inoculation without any clinical signs of disease. The previously inoculated mice ... 75% of mice, while two doses protected 88%. "We report that oral inoculation with a Y. pseudotuberculosis strain, selected for its very low ...

NOVAVAX and the University of Massachusetts Medical School Announce Preclinical Study Results for a Respiratory Syncytial Virus ('RSV') Vaccine Candidate

... of mice that had been immunized with either RSV VLPs via intraperitoneal inoculation or live RSV via intranasal inoculation received a vaccine booster dose and were challenged with live RSV ...

Tips from the journals of the American Society for Microbiology

... the mouse intestine for 2 months following intragastric and subcutaneous inoculation without any clinical signs of disease. The previously inoculated mice ... 75% of mice, while two doses protected 88%. "We report that oral inoculation with a Y. pseudotuberculosis strain, selected for its very low ...

Tips from the journals of the American Society for Microbiology

... the mouse intestine for 2 months following intragastric and subcutaneous inoculation without any clinical signs of disease. The previously inoculated mice ... 75% of mice, while two doses protected 88%. "We report that oral inoculation with a Y. pseudotuberculosis strain, selected for its very low ...

Viroblock SA Reports Positive Results of Preclinical Studies in Antiviral Nasal Spray

... and were conducted by Retroscreen Virology Ltd, a recognized leader in the research and testing of antiviral compounds and vaccines. Both the direct inoculation and the natural transmission protocols have confirmed the effectiveness and the value of the antiviral nasal spray. These in-vivo campaigns ...

New study indicates smallpox vaccination effective for decades

... of smallpox exposure. This may be a critical question because the availability of smallpox vaccines is limited and currently inadequate for a mass inoculation program. We found that vaccinated subjects maintain what appear to be protective levels of neutralizing antibodies to vaccinia indefinitely and do ...

Copan Announces the Appointment of Bob Cooper as Vice President of Business Development in the Pacific Rim

... patented Flocked Swabs, Universal Transport Medium and ESwab. The company manufactures a comprehensive line of laboratory consumables comprising of inoculation loops, needles, spreaders and transfer pipettes. Copan has recently launched WASP: Walk-Away Specimen Processor, a new revolutionary system for ...

New study indicates smallpox vaccination effective for decades

... of smallpox exposure. This may be a critical question because the availability of smallpox vaccines is limited and currently inadequate for a mass inoculation program. We found that vaccinated subjects maintain what appear to be protective levels of neutralizing antibodies to vaccinia indefinitely and do ...

Sinovac Receives Healive(R) and Bilive(R) Purchase Orders from Provincial CDCs for Earthquake Disaster Areas

... Sinovac donated an additional 8,000 doses of Healive(R) to affected areas in the Shanxi Province. Sinovac's clinical experts were dispatched to the inoculation area to assist the local CDCs with immunization. Mr. Weidong Yin, Chairman, CEO and President of Sinovac, commented, "The delivery of additional ...

Anavex 7-1037 reduces rate of cancer tumor growth 69% in pre-clinical studies, compared to currently marketed drug Dacarbazine showing no measurable activity

... drug. These noteworthy results were achieved after ANAVEX 7-1037 had been administered seven times at a statistically significant level (post inoculation day of cancer cells, or p.i.d.=33, tumor size, or p<=0.001) when compared to the tumors of the untreated control mice. The 70 mg/kg dose of ANAVEX ...

Tips from the Journals of the American Society for Microbiology

... infects bacteria. In the study researchers identified the phage, MSa, and tested its activity against S. aureus in mice. Following simultaneous inoculation with both MSa and lethal and non-lethal doses of S. aureus results showed MSa rescued ninety-seven percent of mice from death and fully cleared mice ...

Inoculation of Viral RNA and cDNA to Potato and Tobacco Plants Using the Helios Gene Gun

... PDS-1000/He system is efficient for inoculation of detached leaves, but the Helios gene gun enables inoculation of an intact plant. In this study we ... be used as a readily observed measure for optimal inoculation conditions. The bombardment parameters ...

Fundamentals of Fermentation: Techniques For Benchtop Fermentors

... The protocol for this is the same as for inoculation as described below. inoculation can be performed by aseptically pouring liquids into the vessel through the inoculation port. However we normally use the harvest port to ...

Single-Cell Complementation of Barley Mlo Mutants Using a PDS-1000/He Hepta System

... l 100% ethanol. Conditions for Particle Bombardment and Fungal inoculation Each of the 7 macrocarriers of the Hepta adaptor was loaded ... Microscopic fungal growth was visible 34 days after inoculation as the formation of aerial hyphae and conidiophores. In the ...

FERMENTATION BASICS

... and the metabolite concentration generally change constantly as a result of the metabolism of the cells. After the inoculation of a sterile nutrient solution with microorganisms and cultivation under physiological conditions, four typical phases of growth are observed as ...

E. coli Fermentation Using a BioFlo 110 Bench-Top Fermentor

... suggesting that other factors were limiting. Figures 5 and 6 show increasing OD600nm and dry cell weight (DCW) from the time of inoculation through EFT 4.5-5 hours, consistent with the DO graphs. This run entered exponential growth phase upon inoculation. The short to non-existent ...

Detection of Reporter Gene Activity in Cell Cultures and Murine Epidermis After Helios Gene Gun-Mediated Particle Bombardment

... 1.0 m gold microcarriers. Mice bombarded with the pCMV plasmid represented the negative control. One week after inoculation the skin was removed, embedded in OCT, and quick frozen using liquid nitrogen. Every fifth 10 m air-dried section was ...

Sensitive detection of tumor cells in peripheral blood of carcinoma patients by a reverse transcription PCR method

... 2100 bioanalyzer and the DNA 500 LabChip kit. inoculation system Cell spiking experiments with different ... were performed to test the potential sensitivity of the assay. After inoculation and immunomagnetic enrichment, two carcinoma cells were detected in ...

A membrane-lid tube for bacteria cultivation and freeze-drying

... flasks. To ensure an identical initial bacteria concentration in the different test tubes, LB (Luria Bertani) medium was given a 1% inoculation of overnight culture. After being mixed thoroughly, 1 ml bacteria culture each was transferred to 1.5 ml membrane-lid tubes ...

Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit

... The Eppendorf Thermomixer R (equipped with 2.0 ml Thermoblock) was used for incubation of the LidBac tubes on the bench top after inoculation with respective media. The incubation period was 8 hours on the Thermomixer R at 37C with a mixing speed of 1,400 rpm. The 15 ...