Hydrolysis in Biological Technology

Solazyme Testing BlueFire Ethanol Cellulosic Sugars in Its Microalgae Renewable Oil Production Process

... Arnold Klann, CEO of BlueFire Ethanol Fuels, Inc. "Our patented acid hydrolysis process allows BlueFire Ethanol to utilize a variety of non-food ... to deploy a commercially ready, patented and proven Concentrated Acid hydrolysis Technology Process for the profitable conversion of cellulosic ("Green ...

BlueFire Ethanol To Present at BIO 2009 International Convention

... About BlueFire Ethanol Fuels BlueFire Ethanol Fuels, Inc. was established to deploy a commercially ready, patented and proven Concentrated Acid hydrolysis Technology Process for the profitable conversion of cellulosic ("Green Waste") waste materials to ethanol, a viable alternative to gasoline. BlueFire ...

Genencor Launches Accellerase(R) 1500, Next Step Toward Scale-Up of Cellulosic Ethanol

... on Genencor's patented gene expression technology and the division's 25-year history of biofuels research. Accellerase(R) is critical to the hydrolysis step of converting biomass to ethanol or other biochemicals. In this step, plant material is broken down into sugars for fermentation to ethanol. ...

Verenium Announces First Commercial Cellulosic Ethanol Project

... production utilizes non-food, plant biomass as its feedstock source. The biomass is first broken down into fermentable sugars using acid or enzymatic hydrolysis and industrial enzymes, after which the sugars are fermented into ethanol using various fermentation organisms. Ethanol produced from cellulosic ...

Increased Capacity For Porcine Liver Esterase

... urine infection. Porcine liver esterase is also used on a large commercial scale as a biocatalyst in the resolution of mixtures of chiral esters by hydrolysis to produce optically active carboxylic acids. Lee Biosolutions growth for porcine liver esterase is attributed to meeting stringent customer ...

Genencor Launches First Ever Commercial Enzyme Product for Cellulosic Ethanol

... on a variety of feedstocks. -- Ability to operate in simultaneous saccharification and fermentation (SSF) processes, two step sequential hydrolysis and fermentation (SHF) processes or hybrids of the two. -- High beta-glucosidase activity to minimize residual cellobiose, which may lead to ...

Homogeneous, chemiluminescent kinase assay kits for assessing the functional activity of a broad range of serine-threonine and tyrosine kinases

... fragment complementation and the subsequent hydrolysis of chemiluminescent substrate, HitHunter™ ... active tetrameric β-gal (Fig 1). Subsequent hydrolysis of luminescent substrate generates a signal that ... complement EA and form active enzyme. Subsequent hydrolysis of the chemiluminescent substrate by β-gal ...

Rapid SNP Genotyping of the Pharmacogenomically Important Allele CYP2D6*4 Using Real-Time qPCR on the DNA Engine Opticon 2 System

... the CYP2D6*4 allele in 211 patient samples. Two hydrolysis probes, one labeled with VIC and the other ... In this assay, two differentially labeled hydrolysis probes were used to discriminate ... 2 system. The protocol uses two hydrolysis probes labeled with two different ...

Translate More Protein with Higher Biological Activity

... was assayed for -gal by colorimetric ONGP hydrolysis or for luciferase by luminescence. ... assayed for -gal by colorimetric ONGP hydrolysis assay and rhodanese by the colorimetric assay as ... was assayed for by colorimetric ONGP hydrolysis assay. Rhodanese was assayed by the ...

Brilliant Core Reagent Kits for QPCR and QRT-PCR

... identical primers and linear hydrolysis probe, and a passive reference dye. ... analysis. The kits are compatible with linear hydrolysis (TaqMan probes), molecular beacon, single dye ... with either molecular beacons or a linear hydrolysis probe contained the following components: 1X ...

Gq-coupled Receptor Assays Using FLEXstation

... (1). Characterization of cP2Y 11 by phosphoinositide hydrolysis assay. The receptor was previously characterized, and its agonist ... value for ADP was approximately 190 M as determined by phosphoinositide hydrolysis assay (1, Fig. 2). ...

Fluorometric Protease Assays in the SpectraMax Gemini Microplate Spectrofluorometer: Example Using Caspase3 (MaxLine Application Note #35)

... peptide substrates can interfere with the measurement of their own hydrolysis products. If the emission spectra overlap, the assay may have a high ... 1989. Synthesis of 7amino4methylcoumarin (AMC) derivatives and their hydrolysis by plant cysteine proteinases. J. Chem. Tech. Biotechnol. 46: 1126. ...

Rapidly Prepare Probes and Markers for Small RNA Analysis

... 5' end labeled RNA probe in a ~10 minute alkaline hydrolysis reaction. Complete Kit for Small RNA ... only). Gel Loading Buffer, RNase-free Water, Alkaline hydrolysis Buffer and a manual with detailed protocol and ...

Using XL1-Red Mutator Strain to Generate Esterase Variants

... 1 or 2, and indicators (crystal violet and neutral red). Upon hydrolysis of substrates 1 or 2, the pH in the microenvironment of the ... (wild type and selected mutants) were subjected to the preparative hydrolysis of substrate 1 in phosphate buffer (50 mM, pH 7.5) at 40C. As expected, ...

ANALYSIS OF AMINO ACIDS BY REVERSED PHASE CHROMATOGRAPHY WITH PRECOLUMN DERIVATISATION AND UV/VISIBLE DETECTION

... a UV detector set at λ=338nm. Sample treatment: Samples are digested in acid medium to the complete hydrolysis of the protein fraction. After extraction and washing, the hydrolised amino acids are filtered and vialed, ready for analysis. Derivatisation ...

A Microtiter-based Assay for the Determination of ID50s of b-lactamase Inhibitors Employing Reporter Substrates Detected at UV or Visible Wavelengths (MaxLine Application Note #20)

... be added last. RESULTS Figure 3 shows an assay microtiter plate set up to record the ID 50 s of 8 compounds. The rate of hydrolysis of reporter substrate is shown for each well (column 1 = blank, column 2 = control, columns 3 - 12 = range of inhibitor concentrations). This ...

Screening of Corticosteroids in Urine by Negative Atmospheric Pressure Chemical Ionization LC/MS/MS

... and 25 μL beta-glucuronidase are added, the mixture is vigorously vortexed and kept for 1 hour at 50 C for enzymatic hydrolysis. (The enzymatic hydrolysis step is needed since the corticosteroids are mainly excreted in a conjugated form with the glucuronic acid). Adding 750 μL of 20% buffer K 2 CO ...

High sensitivity quantitation of metabolites of nitrofuran antibiotics in animal tissue using LC/MS/MS

... antibiotics in chicken tissue. Abstract The animal meat samples were prepared by homogenization, organic solvent washes, hydrolysis and derivatization followed by liquid-liquid (L-L) extraction before analysis. The limits of detection were 0.05-0.15 ng/g animal tissue using a ...

Translate High Yields of Biologically Active Proteins

... 2 (CA2): Esterase activity of CA2 was monitored by the net increase in absorbance at 348 nm in a p-nitrophenyl acetate hydrolysis assay [3]. Caspase 3 (CASP3): Catalytic activity was determined by measuring the proteolytic cleavage of the fluorogenic substrate ...

Translate High Yields of Active Protein

... by Sorbo [2], Luciferase activity by luminescence, GFP activity by fluorescence, and -galactosidase by colorimetric ONGP hydrolysis assay Human Protein Expression ActivePro's versatility with a wide range of ...

The World's Best DNase Improved TURBO DNA-free

... the DNase inactivation resin also binds and removes divalent cations, such as Mg 2+ , that can induce RNA hydrolysis when the sample is heated. This is particularly important for researchers who need to heat denature RNA ...

Trouble-shooting: Low yield

... Suggestions: Always store nucleotide stock solutions at a concentration of at least 10 mM, 100 mM is best. Significant hydrolysis occurs after storing at 1 mM for 2 months. Dissolve NTPs or dNTPs in water at an expected concentration of 10 mM. Using 0.05 M ...

Transfection Reagent Selector Kit Handbook

... The prokaryotic enzyme b-galactosidase can be assayed colorimetrically using the substrate o-nitrophenyl-b-D-galactopyranoside (ONPG). The hydrolysis of ONPG by b-galactosidase yields a yellow-colored product, o-nitrophenol, which can be measured photometrically. ...

TripleMaster PCR SystemThe Ideal Choice for Long-Range PCR

... degradation during exposure to high temperatures in buffers that are unable to maintain pH. This is due to depurination, the hydrolysis of the N-glycosidic bond between the base and the sugar of a DNA molecule. Although depurination is a rare event, occurring ...

Genetic Reporter Systems

... of the CAT enzyme which has been expressed. -Galactosidase The prokaryotic -galactosidase naturally catalyzes the hydrolysis of -galactosides (e.g. lactose). However, the use of non-physiological substrates also enables the quantification of ...

Genetic Reporter Systems

... of the CAT enzyme which has been expressed. -Galactosidase The prokaryotic -galactosidase naturally catalyzes the hydrolysis of -galactosides (e.g. lactose). However, the use of non-physiological substrates also enables the quantification of ...